PHYTOCHEMICAL ANALYSIS OF THE WHOLE PLANT OF Mimosa pudica ( Linn . ) 1
Prosanta Pal2015
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Abstract
Chemical diversity in natural product is an immensely rich source of new pharmaceuticals, cosmetics, agrochemicals and other economically important chemicals. Therapeutic potentials of herbal drug ranges from parts of plants, through simple extracts to isolated active constituents. Phytochemical evaluation compresses of different chemical tests and chemical assay. The isolation, purification and identification of active constituents are chemical methods of evaluation. The phytochemical evaluation also covers phytochemical screening carried out for establishing chemical profile of crude drug. The purity of crude drugs is ascertained by quantitative estimation of active chemical constituents present in them. The method may be useful in determining single active constituents or the group of related constituents present in the same drug. The spectrophotometric analysis using UV/ visible/ IR/ Fluorescence/ NMR/ Mass/ X-ray diffraction are physical methods of assay. The modern techniques ...
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PHYTOCHEMICAL ANALYSIS OF THE WHOLE PLANT OF Mimosa pudica (Linn.)
Mimosa pudica (Linn.) is a creeping annual or perennial herb. It has been identified as lajjalu in Ayurveda and has been found to have antiasthmatic, aphrodisiac, analgesic, and antidepressant properties. M. pudica is known to possess sedative, emetic, and tonic properties, and has been used traditionally in the treatment of various ailments including alopecia, diarrhea, dysentery, insomnia, tumor, and various urogenital infections. As the whole plant being used to cure various diseases so we have extracted all the plant constituents from whole plant powder of Mimosa pudica (Linn.) using 50% ethanol following cold maceration technique. Preliminary phytochemical evaluation showed the presence ofcarbohydrates, alkaloids, proteins, amino acids, tannins, phenolics, flavonoids, steroids, fixed oil, mucilage and saponins. Among these compounds alkaloid was found to exhibit different pharmacological properties. So later we have separated and identified the alkaloids present in the 50% ethanolic whole plant extract and its fractions.
Qualitative and Quantitative Phytochemical Screening of Mimoso Pudica Plant Extracts (Touch Me Not)
2014
The present research was carried out to evaluate the phytochemical constituent of aqueous, ethanol and methanol extracts of the leaf, stem and the mixture of the leaf and stem of Mimosa pudica plant. The phytochemical screening which involves both the qualitative and quantitative analysis revealed the presence of secondary metabolites; alkaloids, tannins, glycosides, terpenes, flavonoids and volatile oils in all the three extracts evaluated. There was absence of steroids, anthaquinone and anthaquinone glycosides in the leaf extracts of M. pudica. The leaf extract of M. pudica in ethanol showed no presence of saponins and triterpenoids From the results of Quantitative Phytochemical screening, there were significant differences (P<0.05) in the tannins, glycosides and alkaloids content of the leaf, stem and mixture of leaf and stem. The content of flavonoid was higher in the mixture (10 g %) compared to the leaf (9g %) and stem (8g %), while values for saponin was higher in the stem...
ANALYSIS OF PHYTOCHEMICALS AND MIMOSINE FROM MIMOSA PUDICA
Mimosa pudica has been reported to contain mimosine & phytochemical components.The drug is also found to be rich in tannins. The root is also stated to have anti-convulsant activity. Successive extracts of the whole plant are reported to have antimicrobial activity. It was found that the root of Mimosa pudica have acetylcholine esterase inhibitory activity. It majorly possesses antibacterial,antifungal,antivenom, antifertility, anticonvulsant, antidepressant, aphrodisiac, Skin disease,respiratory infections and urinary tract infections .
Preliminary Phytochemical Screning and Antimicrobial Analysis of Mimosa pudica (Lin.)
Medicinal properties of plants are the most precious gift of Mother Nature to mankind. India has several traditonal medicinal systems, such as Ayurveda and Unani, which has survived through more than 300 years mainly using plant based drugs. The primary benefits of using plant derived medicines are that they are relatively safer than synthetic alternatives, ofering profound therapeutic benefits and afordable treatment. The Mimosa pudica invites atention of the researchers worldwide for its pharmacological activity such as anti diabetic, antioxin, antihepatotoxin, antioxidant and wound healing activity. Acording to diferent researches done, Mimosa Pudica is used to relax the mind and relieve depresion, mental distres, iritabilty, severe palpitations and amnesia. It is a mod enhancer and improves circulation of blod. The present investigation was caried out to identify the phytochemicals present in Mimosa pudica and to study the antimicrobial activity shown by various extracts of the plant. Phytochemical screning of various extracts of Mimosa pudica showed the presence of secondary metabolites such as alkaloids, tanins, saponins, flavonoids etc. The methanolic and ethanolic extracts showed beter antimicrobial activity. Keywords: Phytochemicals, Mimosa pudica, Secondary metabolites
Quantification of Phytochemicals and Metal Ions as well as the Determination of Volatile Compounds, Antioxidant, Antimicrobial and Antacid Activities of the Mimosa pudica L. Leaf: Exploration of Neglected and Under-Utilized Part
Wiley, 2023
Mimosa pudica L. (MP) is well-known plant in traditional medicinal system, especially in India. Unfortunately, leaves of MP are less explored. To determine the food and nutritional value of the neglected part of Mimosa pudica L. (MP), that is MP leaves, phytochemicals and metal ions of MP were quantified by newly developed HPLC and ICPOES-based methods. The content of phytochemicals observed using HPLC analysis for chlorogenic acid, catechin, and epicatechin was 141.823 (� 8.171), 666.621 (� 11.432), and 293.175 (� 12.743) μg/g, respectively. Using GC/MS/MS analysis, fatty acid like oleic acid were identified. In ICP-OES analysis, a significant content of Na, K, Ca, Cu, Fe, Mg, Mn, and Zn was observed. The observed TPC and TFC for MP leaf extracts was 44.327 (� 1.041) mg GAE/ g of wt. and 214.217 (� 4.372) mg QCE/ g of wt., respectively. The DPPH assay depicted a strong antioxidant activity of MP leaf extracts with IC 50 values of 0.796 (� 0.081) mg/mL and a TEAC value of 0.0356 (� 0.0003). A significant antacid activity (666 mg MP + 400 mg CaCO 3 > 400 mg CaCO 3 @ 666 mg Gelusil) of MP leaves was noticed. The methanolic extract of MP leaves demonstrated anti-microbial activity against Staphylococcus aureus (15 � 2mm), Pseudomonas aeruginosa (12 � 2mm) and Escherichia coli (10 � 2mm). In silico studies confirmed the in vitro results obtained for antioxidant, antiacid, and antimicrobial activities. In addition, in silico studies revealed the anti-cancerous and anti-inflammatory potential of the MP leaves. In summary, this study demonstrated the medicinal significance of MP leaves and the conversion of agro-waste or the under-utilized part of MP into pharmaceutical potent materials. Consequently, the present study highlighted that MP leaves alone have medicinal importance with good nutritional utility and possess large promise in the pharma industry along with improving bio-valorization and the environment.
A REVIEW ON THE PHARMACOLOGICAL AND TRADITIONAL PROPERTIES OF MIMOSA PUDICA Review Article
International Journal of Pharmacy and Pharmaceutical Sciences, 2019
Consumption of fruits and vegetables fight against oxidative damage by inhibiting or reducing free radicals and reactive oxygen species. Mimosa pudica is a perennial herb and belongs to the family Fabaceae. Epidemiological studies have shown that M. pudica contains metabolites such as phenols and flavonoid compounds which possess pharmacological properties such as antidiabetic, antimicrobial, antiulcer, antidepressants and anti-inflammatory. Ecological studies have shown that M. pudica grows in all types of soil which can survive in soil with low nutrient concentration. It usually requires disturbed soil to establish itself. It is commonly seen in the wastelands and along roadsides, which is an ethnomedical plant that may be used in managing various types of disease. Valuable information and literature on M. pudica are analysed and consulted using a different database such as Google Scholar, Google, Science Direct, Web of Science, Academic Journals and Pubmed. This review article summarises the pharmacological properties of M. pudica.
Preliminary Pharmacognostic Evaluations and Phytochemical Studies on Roots of Mimosa Pudica (Lajvanti)
2010
The roots of Mimosa pudica Linn (Mimosae) are reported to have great medicinal value. Pharmacognostic evaluation including examinations of morphological and microscopic characters, ash value, powder analysis, and extractive values were carried out. Phytochemical screening including qualitative chemical examinations was also carried out.
Preliminary Pharmacognostic and Phytochemical Evaluation of Stems of Mimosa hamata (Willd)
International Journal of Scientific Research in Science and Technology, 2019
"Objective The aim of the present study is to investigate the pharmacognostic and phytochemical investigation of the Mimosa hamata (Willd.) is a flowering shrub of Mimosaceae family which is used in various traditional medicines to cure various diseases. Mimosa hamata (Willd.) and Mimosa pudica are also known as Touch-me-not plant. A wide range of chemical compounds including 4-ethyl-gallic acid; triterpinicsaponin A, B; ethylgallate; mimonoside A, B, C; etc have been isolated from this plant. Methods Morphological and microscopic characters, powder analysis, and extractive values of ethanolic extract of stem of Mimosa hamata and qualitative estimation of phytochemicals were determined. The pharmacognostical parameters such as total ash value, acid insoluble ash value and water soluble ash value, alcohol soluble extractive and water soluble extractive were also determined. Results The results of pharmacognostic analysis of stem of Mimosa hamata (Willd) have revealed the total ash 8.5 % , water soluble ash 0.5 %, water insoluble ash 1.5%, Moisture content 2.5 %, alcohol soluble extractive value 14.29 % and water soluble extractive value 9.75%. The preliminary phytochemical analysis of stem of showed the presence of flavonoids, carbohydrates, tannins etc. Conclusions It signifies that results revealed the presence of various bioactive constituents which could be exploited for their biopotential for medicinal purposes. "
A Review on the Pharmacological and Traditional Properties of Mimosa Pudica
International Journal of Pharmacy and Pharmaceutical Sciences
Consumption of fruits and vegetables fight against oxidative damage by inhibiting or reducing free radicals and reactive oxygen species. Mimosa pudica is a perennial herb and belongs to the family Fabaceae. Epidemiological studies have shown that M. pudica contains metabolites such as phenols and flavonoid compounds which possess pharmacological properties such as antidiabetic, antimicrobial, antiulcer, antidepressants and anti-inflammatory. Ecological studies have shown that M. pudica grows in all types of soil which can survive in soil with low nutrient concentration. It usually requires disturbed soil to establish itself. It is commonly seen in the wastelands and along roadsides, which is an ethnomedical plant that may be used in managing various types of disease. Valuable information and literature on M. pudica are analysed and consulted using a different database such as Google Scholar, Google, Science Direct, Web of Science, Academic Journals and Pubmed. This review article su...
QUALITATIVE AND QUANTITATIVE ESTIMATION OF BIOACTIVE COMPOUNDS IN MIMOSA HAMATA
The present study aimed to investigate the qualitative and quantitative analysis of the major bioactive constituents of medicinally important plant Mimosa hamata in its ethanolic and methanolic extract of whole parts of plant.
Research Article IN VIVO PHARMACOLOGICAL INVESTIGATION OF MIMOSA PUDICA L
2013
Objective: To evaluate the anti-nociceptive, acute toxicity, gastro intestinal motility, anti-pyretic investigations of leaf extract of Mimosa pudica L. leaves in Swiss albino mice following oral administration. Methods: In-vivo anti-nociceptive activity test was evaluated by tail immersion test. In-vivo acute toxicity test was conducted using acute toxic class method. In-vivo gastrointestinal motility was determined by charcoal feces defecation time. In-vivo antipyretic activity test was evaluated by brewer’s yeast induced pyrexia. Results: In-vivo anti-nociceptive activity test shows that methanol & ethanol extracts (250 & 500 mg/kg b.w.) performed significant activity (p<0.05) in mice comparing to the standard drug diclofenac Na. In-vivo acute toxicity test was done on mice with methanol, ethanol and chloroform extracts (2000, 1000, 500 mg/kg b.w.) of Mimosa pudica leaf and no reaction or death occurred in mice during two weeks of observation. In-vivo gastrointestinal motility...
A Recent Review on Phytochemical Constituents and Medicinal Properties of Mimosa rubicaulis Lam
International Journal of Scientific Research in Science and Technology, 2019
In the developing world the trend has been changed from synthetic to natural medicine. Trees and plants are sources of many medicines. The use of medicinal plants was compiled in Ayurveda which listed more than 8000 herbal remedies. Mimosa rubicaulis Lam belonging to the family Fabaceae and subfamily Mimosoideae. It is bipinnately compound, each leaf having 8–12 pairs of pinnae, each with 16–20 pairs of pinnules, unlike this plant is having good potential activity in plant due to it has been attributed to their flavanoid and sterol contents. Several flavanoids isolated from these plants have been discovered to possess significant Various Pharmacological activity. Literature review reports the presence of flavanoids, triterpenoids, phytosterols and alkaloids in Mimosa rubicaulis Lam.
Secondary Metabolites from Mimosa Pudica: Isolation, Purification and NMR Characterization
Aim: isolation, purification and NMR characterization of secondary metabolites in Mimosa pudica. Study design: extraction, isolation and identification of the secondary metabolties from the petroleum ether and metabolites extracts from Mimosa pudica. Methodology: In the course of our study on Mimosa pudica, a compound was isolated and purified using different chromatographic techniques and the structure of the compound determined on the basis of their spectroscopic data (IR, HNMR, COSY, DEPT, MS). Place and Duration of Study: this work was carried out in the chemistry Department Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria within the month June 2016 to December 2016. Result: The entire plant parts of Mimosa was extracted using absolute ethanol, the TLC of the crude extract indicate the many compounds with Rf values 0.41,0.43,0.56,0.68,0.89 and 0.90. Purification: The results of several chromatographic techniques on the isolated gave rise to a triterpenoid-glycoside with Rf value 0.55. The spectral analyses involving HNMR, NMR, DEPT, 135 COSY, HMBC and IR, MS were useful in proposing the structure of the compound MP11.
Phytochemical Analysis and Anti Microbial Activity of Mimosa pudica Linn.
Ethanolic extracts of Mimosa pudica leaves were screened for phytochemical constituents and antimicrobial activity towards pathogens i.e. bacteria and fungi. The activity was tested against Bacillus subtilis, Pseudomonas aeruginosa, Klebsiella pneumonia, Aspergillus flavus and Trycophyton rubrum at different concentrations of 25, 50, 75 and 100 μl/ disc and the results have been illustrated. Phytochemical analysis of the extract revealed that the antimicrobial activity of the plant materials is due to the presence of active constituents like alkaloids or tannins. Key words: Mimosa pudica, antimicrobial activity, phytochemical.
IN VIVO PHARMACOLOGICAL INVESTIGATION OF MIMOSA PUDICA L.
Objective: To evaluate the anti-nociceptive, acute toxicity, gastro intestinal motility, anti-pyretic investigations of leaf extract of Mimosa pudica L. leaves in Swiss albino mice following oral administration. Methods: In-vivo anti-nociceptive activity test was evaluated by tail immersion test. In-vivo acute toxicity test was conducted using acute toxic class method. In-vivo gastrointestinal motility was determined by charcoal feces defecation time. In-vivo antipyretic activity test was evaluated by brewer’s yeast induced pyrexia. Results: In-vivo anti-nociceptive activity test shows that methanol & ethanol extracts (250 & 500 mg/kg b.w.) performed significant activity (p<0.05) in mice comparing to the standard drug diclofenac Na. In-vivo acute toxicity test was done on mice with methanol, ethanol and chloroform extracts (2000, 1000, 500 mg/kg b.w.) of Mimosa pudica leaf and no reaction or death occurred in mice during two weeks of observation. In-vivo gastrointestinal motility test indicates significant (p<0.01) increase in gastrointestinal motility by ethanol extracts of (250 & 500 mg/kg b.w.) comparing to the standard drug loperamide. In-vivo antipyretic activity test shows that methanol (250 & 500 mg/kg b.w.), ethanol (250 & 500 mg/kg b.w.) and chloroform (250mg/kg b.w.) extracts showed significant (p<0.05) reduction in temperature of mice comparing to the standard drug paracetamol. Conclusion: The result of the study indicates analgesic, antipyretic properties along with gastrointestinal motility stimulating effects. According to the acute toxicity study, the leaf extracts are safe up to 2000 mg/kg in-vivo concentration.
Evaluation of antioxidant and cytotoxic activity of methanolic extract of Mimosa pudica leaves
The Pharma Innovation Journal, 2014
1. Department of Pharmacy, Primeasia University Dhaka, Bangladesh, HBR Tower, Kamal Ataturk Avenue, 9 Banani C/A, Dhaka-1213, Bangladesh. 2. Department of Pharmacy, School of Science, Primeasia University, HBR Tower, Kamal Ataturk Avenue, 9 Banani C/A, Dhaka-1213, Bangladesh. 3. Department of Pharmacy, School of Science, Primeasia University, HBR Tower, Kamal Ataturk Avenue, 9 Banani C/A, Dhaka-1213, Bangladesh. 4. Department of Pharmacy, School of Science, Primeasia University, HBR Tower, Kamal Ataturk Avenue, 9 Banani C/A, Dhaka-1213, Bangladesh. 5. Department of Pharmacy, School of Science, Primeasia University, HBR Tower, Kamal Ataturk Avenue, 9 Banani C/A, Dhaka-1213, Bangladesh.
Preliminary Phytochemical Screening, Total Phenol and Flavonoid Content of Mimosa Rubicaulis and Reinwardita Indica
International Journal of Pharmacy and Pharmaceutical Sciences
Objective: Phytochemicals as phenol and flavonoid have a powerful biological activity. So, this study aimed to carry out phytochemical screening, total phenol and flavonoid content in two plant species i.e. M. rubicaulis and R. indica. Methods: The extraction of different parts of two plant species was done by maceration using ethanol. Phytochemical screening was done to confirm the presence of phytochemicals. Total phenol content was done by Folin ciocalteu method and total flavonoid content was done by Aluminium chloride colorimetric method. Results: Phytochemical analysis revealed the presence of flavonoid, phenol, terpenoids in both plant species. The highest concentration of phenol content was observed in the root and stem of an extract of M. rubicaulis i.e. 281.83±1.98 mg GAE/g dry extract weight and 225.37±0.60 mg GAE/g dry extract weight. The highest concentration of flavonoid contents was observed in the leaves of R. indica i.e. 462.21±4.67 mg QE/g dry extract weight follow...
PRELIMINARY PHYTOCHEMICAL SCREENING, TOTAL PHENOL AND FLAVONOID CONTENT OF MIMOSA RUBICAULIS AND REINWARDITA INDICA Original Article
International Journal of Pharmacy and Pharmaceutical Sciences, 2020
Objective: Phytochemicals as phenol and flavonoid have a powerful biological activity. So, this study aimed to carry out phytochemical screening, total phenol and flavonoid content in two plant species i.e. M. rubicaulis and R. indica. Methods: The extraction of different parts of two plant species was done by maceration using ethanol. Phytochemical screening was done to confirm the presence of phytochemicals. Total phenol content was done by Folin ciocalteu method and total flavonoid content was done by Aluminium chloride colorimetric method. Results: Phytochemical analysis revealed the presence of flavonoid, phenol, terpenoids in both plant species. The highest concentration of phenol content was observed in the root and stem of an extract of M. rubicaulis i.e. 281.83±1.98 mg GAE/g dry extract weight and 225.37±0.60 mg GAE/g dry extract weight. The highest concentration of flavonoid contents was observed in the leaves of R. indica i.e. 462.21±4.67 mg QE/g dry extract weight followed by stem and root of M. rubicaulis i.e. 381.06±5.23 mg QE/g dry extract weight and 337.43±1.39 mg QE/g dry extract weight. Conclusion: Phytochemical analysis concluded the presence of biologically important phytoconstituents like flavonoid and phenol in both plant species. Further studies, should be carried out to isolate specific chemical constituents and should be used in different studies to explore their biological effects.
Cytotoxicity, Antimicrobial and Antioxidant Studies of the Different Plant Parts of Mimosa Pudica
Stamford Journal of …, 2009
The petroleum ether, chloroform and methanol crude extracts of the two different plant parts (aerial part and root) of Mimosa pudica (Mimosaceae) were screened in vitro for cytotoxicity studies by brine shrimp lethality bioassay and antimicrobial screening by disc diffusion ...
Non-clinical and pre-clinical pharmacological investigations of Mimosa diplotricha
2015
The present study aims to screen pharmacological and phytochemical screenings of the Mimosa diplotricha . For this the ethanolic crude extract of M. diplotricha (EMd) was undergone for checking antioxidant, anti-inflammatory and membrane stabilizing, anti-diarrheal and clotlysis activities followed by a preliminary phytochemical screening. Results suggest that, the EMd concentration-dependently exhibited antiradical (DPPH: 1,1-diphenyl-2-picrylhydrazyl) and anti-inflammatory activities. The EMd also exhibited promising anti-diarrheal effect in Swiss mice, while a negligible clotlysis activity was observed in clotted human blood. Additionally, the phytochemical screening of the crude plant extract revealed the presence of alkaloids, glycosides, flavonoids and reducing sugars. In conclusion, M. diplotricha may be a good source of antioxidant and anti-diarrheal agents. Further researches are recommended for the isolation and characterization of its phytochemicals.
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