Occurrence of Bordetella Infection in Pigs in Northern India

Infection and immunity, 1987

Four strains of Bordetella bronchiseptica (CSU-P-1, 64-C-0406, 1120-A-83-013, and B205BT) with defined virulence for neonatal swine were examined, and an attempt was made to correlate the presence of certain in vitro phenotypic characteristics with the ability of a particular B. bronchiseptica strain to produce turbinate and lung lesions in piglets. All of the strains except CSU-P-1 colonized the nasal passages of the pigs heavily, and strains 1120-A-83-013 and B205BT produced moderate to severe nasal and lung lesions in experimentally infected piglets. All of the strains attached equally well to porcine tracheal ring explant cultures, and all of the strains except CSU-P-1 produced smooth, hemolytic colonies on Bordet-Gengou medium, agglutinated porcine erythrocytes, and possessed adenylate cyclase activity. Strains 1120-A-83-013 and B205BT produced considerably higher levels of dermonecrotic toxin activity than did strains CSU-P-1 and 64-C-0406. These results indicate that producti...

Vaccine, 1984

The guinea-pig skintest was used to test the pathogenic character of nine Bordetella bronchiseptica isolates derived from a number of different animals. One isolate was non-pathogenic, one showed a doubtful reaction whereas the others were pathogenic. Analysis of the cell envelope protein patterns by sodium dodecyl sulphate polyacrylamide gel electrophoresis revealed only minor differences. Two major protein bands and at least thirty five minor bands were observed. The major protein with an apparent molecular weight of 37000 has properties similar to those of pore proteins of Enterobacteriaceae. Lipopolysaccharides (LPS), analysed by the same technique, could be separated into two fractions, LPS-I and LPS-II. The electrophoretic mobilities of the LPS of the strains were indistinguishable from each other with the exception of that of the non-pathogenic strain, which also differed serologically from that of the other strains. All sera of animals which had been successfully vaccinated ...

Antimicrobial Agents and Chemotherapy, 2004

MICs for 349 Bordetella bronchiseptica isolates from respiratory tract infections of swine were determined by broth microdilution. The lowest MIC at which 90% of isolates tested are inhibited (MIC90) was that of tetracycline and enrofloxacin (0.5μ g/ml), whereas the highest MIC90s were those of tilmicosin and cephalothin (32 μg/ml) as well as streptomycin (256μ g/ml).

Diagnostic Microbiology and Infectious Disease, 2015

Bordetella bronchiseptica is a well-known veterinary pathogen, but its implication in human disease is probably not fully recognized. The purpose of this study was to determine the clinical significance of 36 B. bronchiseptica isolates from respiratory samples of 22 patients. Therefore, we describe microbiological characteristics, including phenotypic and genotypic identification as well as antimicrobial susceptibilities of the isolates. Clonal relatedness was evaluated using pulsed-field gel electrophoresis (PFGE). Most of the patients had some underlying immunosuppressive condition. Eighteen out of 22 (82%) patients had respiratory symptoms, and the death of 2 patients was associated with respiratory infection.All strains were correctly identified at species level by the simultaneous use of phenotypic methods and were confirmed by specific amplification of the upstream region of the fla gene. Tigecycline, minocycline, doxycycline, colistin, and meropenem were the most active agents tested. PFGE analysis revealed that repeated infections involving each patient had been caused by the same strain. Published by Elsevier Inc.

Research in Microbiology, 1999

Polymerase chain reaction (PCR) assays were developed that enabled not only discriminative detection of three Bordetella species, B. pertussis, B. parapertussis, and B. bronchiseptica (Bspp PCR), but also specific detection of B. bronchiseptica (Bb PCR). An upstream sequence of the flagellin gene was used as a target DNA region. This sequence contained differences in B. pertussis, B. parapertussis, and B. bronchiseptica DNA. These species could then be differentiated using two different sets of primers, Bspp and Bb. When oligonucleotide Bspp primers were used, PCR products were obtained from the three species of Bordetella. A fragment of the expected size (164 bp) was amplified using B. bronchiseptica and B. parapertussis DNA, but a fragment with a distinct molecular weight was amplified with B. pertussis DNA (195 bp). This Bspp PCR was specific and sensitive, but it could not differentiate between B. parapertussis and B. bronchiseptica. When Bb primers were used, a 237-bp PCR product was detected only from B. bronchiseptica DNA. No PCR products were identified after Bb PCR amplification of DNAs either from B. parapertussis isolates or B. pertussis isolates, nor from other respiratory pathogen DNAs tested. This second PCR assay had a sensitivity limit of less than 10 organisms of B. bronchiseptica after detection with a specific probe. The specificity and the sensitivity of the fla PCR assay were evaluated with purified DNA, as was its capacity for detecting the bacteria in human clinical samples and in lungs of infected mice. © Elsevier, Paris

2009

Lung, trachea, and serum samples obtained from turkeys showing clinical signs of respiratory disease were examined for the presence of Bordetella avium infection (bordetellosis) using culture, enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR). The material of this study consisted of 2 live turkeys at the age of 24 weeks with respiratory distress, nasal discharge, and cough that had been brought to the Microbiology Laboratory of the Faculty of Veterinary Medicine, Adnan Menderes University. The results of the study show that B. avium, the causative agent of bordetellosis, was not isolated from either the lung or trachea, while both trachea and lung samples were positive in PCR. The presence of antibodies against B. avium was detected in all serum samples by ELISA. However, sera samples were negative in PCR. In conclusion, it was thought that ELISA is a practical assay while the results of ELISA and PCR were found complementary. This study reported the pre...

Veterinary Microbiology, 2014

Bordetella bronchiseptica is a widespread Gram-negative pathogen occurring in different mammal species. It is known to play a role in the aetiology of infectious atrophic rhinitis of swine, canine kennel cough, respiratory syndromes of cats, rabbits and guinea pigs, and sporadic human cases have also been reported. In this study, ninety-three B. bronchiseptica strains were examined from a broad range of host species and different geographical regions using restriction fragment length polymorphism analysis of polymerase chain reaction products of flaA to reveal the possible host-specificity of the flagellin. Eight types (A-H) of flaA were identified, including five newly described ones (D-H). All but one of the twentytwo B. bronchiseptica strains from swine showed type B fragment pattern. The eighteen Hungarian isolates of canine origin were uniform (type A) while in other countries type B and D were also present in dogs. The sequence and phylogenetic analysis of 36 representative strains of flaA types revealed four clusters. These clusters correlated with flaA PCR-RFLP types and host species, especially in pigs and dogs. The revealed diversity of the strains isolated from human cases indicated possible zoonotic transmissions from various animal sources.

International Journal of Systematic Bacteriology

Amplified ribosomal DNA restriction analysis (ARDRA) in which the almost complete 16s rRNA gene was used as the target of the PCR assay and randomly and repetitive element-primed PCR were used to characterize 67 strains belonging to the family Alcaligenaceae. Particular emphasis was placed on strains of BordetelZa pertussis (13 strains), Bordeteh parapertussis (10 strains), and BordeteZZa bronchiseptica (10 strains) (these organisms were referred to as the B. bronchiseptica group), as well as BordetelZa avium (19 strains). Our data indicate that strains belonging to the B. bronchiseptica group behave as a single species comparable to strains of B. avium. ARDRA allowed us to differentiate among all other species as well but was not useful for infraspecific typing. Randomly and repetitive element-primed PCR could be used to distinguish several infraspecific types within B. avium and the B. bronchiseptica group and allowed us to differentiate these two taxa.

Journal of Comparative Pathology, 1997

Emerging Infectious Diseases, 2009

Bordetella avium is thought to be strictly an avian pathogen. However, 16S rRNA gene sequencing identifi ed 2 isolates from 2 humans with respiratory disease as B. avium and a novel B. avium-like strain. Thus, B. avium and B. avium-like organisms are rare opportunistic human pathogens. S everal Bordetella species have been associated with respiratory disease in humans. Although B. avium is thought to be strictly an animal pathogen that causes tracheobronchitis in wild and domesticated birds (1,2), infections in birds share many of the clinical and histopathologic features of disease in mammals caused by B. pertussis and B. bronchiseptica (3). Human cases of respiratory disease associated with B. avium have only recently been reported in patients with cystic fi brosis (4). We describe 2 isolates, B. avium and a novel strain resembling B. avium, isolated from 2 patients with pneumonia, thereby demonstrating that B. avium and B. avium-like organisms are opportunistic human pathogens.