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The identification of microorganisms is crucial in various fields, including clinical diagnostics and food safety. This study focuses on the identification of an unknown bacterium through differential tests, ultimately concluding that the bacterium is Serratia marcescens. Multiple assays were conducted to analyze structural, culture, and biochemical characteristics, which confirmed the opportunistic pathogenic nature of S. marcescens, particularly in hospitalized patients.
2015
In many scientific studies, researchers have used many unknown bacteria. The time required for reporting and identifying these bacteria must be evaluated. Biochemical tests are a conventional and inexpensive means of identifying bacteria. That is why many researchers prefer to apply these techniques in identifying microorganisms, instead of opting for newer techniques like polymerase chain reaction (PCR). Although identification of bacteria via biochemical tests is a well-known procedure among microbiologists, it consists of many complex procedures, making it very difficult for experts in other fields of study , such as environmentalists, chemists, or even biotechnologists and biologists, to evaluate given the lack of a quickly available, short, and simple reference manual. The goal of this paper is to present a systematic procedure that is completely understandable and applicable for researchers who are not familiar with microbiology or its relevant techniques.
2015
PURPOSE: To provide a forum for discussion of microbiology issues in the pharmaceutical and related industry. The information contained in this newsletter includes the professional opinions of individuals and does not represent the policies or operations of any corporation or government agency to which they may be associated. PMF Newsletter is intended to serve as an open forum. The information in PMF Newsletter is solely for informational purposes and is developed from sources believed to be reliable. Statements expressed constitute current opinions derived through analysis of available information and professional networking. Articles or opinions are for infor-mation only to stimulate discussion and are not necessarily the views of the PMF board or regulatory agencies. The PMF Newsletter cannot make any representations as to the accuracy or completeness of the information presented and the publisher cannot be held liable for errors.
International Journal of Research Publications
Bacteria are pathogenic microorganisms causing a number of diseases in humans from light to life threatening conditions. For proper treatment of patients infected with these diseases require proper diagnosis of diseases causing bacterial agent. As the bacteria are divided into two main as Gram Positive and Gram Negative Bacteria. Both type of bacteria exhibit a number of inherited biochemical properties by which we can differentiate, can check there presence and absence, can check their gram negative and gram positive nature. Therefore, the present review is focused to describe different biochemical tests in one article.
2019
Objective: In this study, three different methods were compared for the identification of some Gram positive and Gram negative reference bacteria. Materials and Methods: For this purpose the identification accuracy rates of Enterococcus faecalis, Enterococcus faecium, Streptococcus agalactiae, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Serratia marcescens were analysed by conventional bacteriological methods, commercial bacterial identification test kit (Microgen™ ID) and automated bacteria identification system (BD Phoenix 100™). Results: As a result of analyses, the identification accuracy rates of examined cultures were 94.5%, 95.2%, 94.6%, 89.6%, 91.1%, 92.5%, 86.9%, 96.4% by conventional bacteriological methods, 93.84%, 89.2%, 98.86%, 96.55%, 97.98%, 95.43%, 86.69%, 92.39% by commercial bacterial identification test kit and 98%, 99%, 99%, 96%, 96%, 97%, 99%, 98% by automated bacteria identification system, respectively. Conclusion: ...
Bacterium, 2022
To identify bacteria via gram staining procuedure and their shape whether it be bacillus, coccus, or spirochete.
2021
Identification of unknown microorganisms in a mixed population through various lab techniques.
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