Comparability of results from two leakage models

International Endodontic Journal, 2002

Kont Çobankara F, Adanir N, Belli S, Pashley DH.

Journal of Endodontics, 2002

A fluid transport model study was used to compare the sealing ability of five root canal sealers (AH26, AH Plus, Apexit, Diaket, and Ketac-Endo) on 60 single-rooted teeth after 1 yr of storage. The root canals were prepared with Gates Glidden drills by using a step-back technique before lateral condensation of gutta-percha with the tested sealers. The specimens were stored in saline solution for 1 yr at 37°C. The leakage was measured by the movement of an air bubble in a capillary glass tube connected to the experimental root section. Apexit (0.490 l) leaked significantly more than AH Plus (0.378 l) and Ketac-Endo (0.357 l), whereas AH26 (0.390 l) and Diaket (0.429 l) showed no significant difference from either Apexit or from AH Plus and Keto-Endo.

Brazilian Dental Science, 2012

Objective: To evaluate the apical leakage exhibited by different endodontic sealers. Material and Methods: (maxillary molars) and distal roots (mandibular molars) sectioned, standardized and instrumented with Mtwo condensation technique and divided into three groups (n=10), according to the endodontic sealer employed: G1-AH Plus, G2-Fill Canal, G3-MTA Fillapex. All sealer. Gutta-percha points were immersed into sodium hypochlorite for 24 h to achieve disinfection. After the

International endodontic journal, 2012

De-Deus G, Brandão MC, Leal F, Reis C, Souza EM, Luna AS, Paciornik S, Fidel S. Lack of correlation between sealer penetration into dentinal tubules and sealability in nonbonded root fillings. International Endodontic Journal ABSTRACT: Aim To determine the correlation between leakage and sealer penetration into dentinal tubule. Methodology The root canal of 60 mandibular central incisor teeth were prepared and filled under standardized conditions. Teeth were placed into a device to assess glucose penetration using 15 psi pressure application. After 1 h, glucose concentrations in the lower chamber were measured using an enzymatic reaction. Each specimen was then sectioned horizontally at 3, 6 and 8 mm from the apex, and a standard metallographic preparation was performed. The coronally facing surface of each slice was examined in a high-resolution stereomicroscope and under Confocal Laser Scanning Microscope. For each slice, the external outline of the root canal, the internal circ...

Journal of Clinical and Experimental Dentistry, 2014

Objectives: The purpose of this in vitro study was to assess the effect of root canal preparation size and taper on the amounts of glucose penetration. Material and Methods: For conducting this experimental study, eighty mandibular premolars with single straight canals were divided randomly into 2 experimental groups of 30 samples each and 2 control groups. Using K-files and the balance force technique, canals in group 1 were prepared apically to size 25 and coronally to size 2 Peesoreamer. Group 2 were instrumented apically and coronally to size 40 and size 6 Peesoreamer, respectively. Rotary instrumentation was accomplished in group 1; using size 25 and .04 tapered and in group 2, size 35 and .06 tapered Flex Master files. Canals were then obturated by lateral compaction of cold gutta-percha. Glucose penetration through root canal fillings was measured at 1, 8, 15, 22 and 30 days. Data were recorded as mmol/L and statistically analyzed with Mann-Whitney U test (P value=. 05). Results: In comparison to group 1, group 2 showed significant glucose leakage during the experimental period (P value < .0001). Also, in each experimental group, the amount of micro-leakage was significantly increased at the end of the study. Conclusions: Under the condition of this study, the amounts of micro-leakage through root canal fillings are directly related to the size and taper of root canal preparation and reducing the preparation size may lead to less microleakage.

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2007

Objective. To compare the sealing ability of gutta-percha and thermoplastic synthetic polymer-based systems along the root canals using a recently introduced glucose penetration model. Study design. Premolars (n ϭ 156) instrumented to an apical size of .06/40 were divided into 12 experimental and 2 control groups. The root canals were filled with either gutta-percha (groups 1-6) or Resilon (groups 7-12) core materials combined with AH Plus (groups 1, 4, 7, 0), Ketac Endo (groups 1, 5, 8, 11), or Epiphany (groups 2, 6, 9, 12), using cold lateral compaction (groups 1-3, 7-9) or System B with Obtura II (groups 4-6, 10-12). The leaked glucose concentration was measured spectrophotometrically at 1, 8, 15, 22, and 30 days. Percentage leaking of each group was also calculated. Data were recorded as mmol/L and statistically analyzed with Kruskal-Wallis and Mann-Whitney U tests (P ϭ .05). Results. Throughout the experimental period Resilon/Ketac-Endo/cold lateral compaction (group 8) showed the highest mean cumulative glucose penetration (mmol/L) (17.27 Ϯ 10.32), whereas Resilon/AH Plus/cold lateral compaction (group 7) had the least (3.36 Ϯ 4.65) (P Ͻ .05). At the 30 th day, the least leaking percentage was observed in guttapercha, Epiphany/cold lateral compaction (group 3). Glucose penetration was affected by the obturation technique in some groups. Ketac-Endo either with gutta-percha (group 2) or Resilon (group 8) indicated significantly less glucose penetration in warm technique, whereas gutta-percha/Epiphany (group 3) had significantly less glucose penetration in cold technique (P Ͻ .05). Conclusions. All material/technique combinations allowed glucose penetration. Gutta-percha/AH Plus combinations allowed similar patterns of glucose penetration to Resilon/Epiphany combinations. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2007;104:e66-e73)

Journal of Endodontics, 1991

Previous studies have shown that lateral condensation of gutta-percha and sealer can provide an adequate apical seal. The purpose of this study was to compare the level of apical dye penetration when different sealers were used. One-hundred twentyfive teeth with single root canals were biomechanically prepared using the step-back technique and irrigation with EDTAC and 1% NaOCI solutions. The teeth were divided into five groups of 25 teeth each. The control group root canals were filled with laterally condensed gutta-percha and no sealer and the other four groups were filled with laterally condensed gutta-percha and either Apexit, Sealapex, Tubli-Seal, or AH26 sealer. After storage in 100% humidity at 37~ for 48 h, the root surfaces were coated with nail varnish (except at the apex) and placed in 2% methylene blue dye solution and centrifuged at 3 x g for 3 min. The roots were sectioned transversely at 1-mm intervals to determine the following mean levels of dye penetrations: Apexit, 1.67 mm; Sealapex, 2.28 mm; Tubli-Seal, 1.95 mm; AH26, 0.82 mm; and gutta-percha alone, 8.37 mm. This study demonstrated that a root canal sealer should be used in conjunction with laterally condensed gutta-percha and that AH26 sealer provides a significantly better apical seal than the other sealers.

2020

The objective of this study was the evaluation and comparison of coronal bacterial leakage of two different instrumentation techniques and two endodontic sealers Apexit Plus and EndoRez. Sixty-eight human teeth caries free were tested in this study. Cleaning and shaping was carried out with step-back and crown-down technique and irrigated with 2% NaOCl and 17% EDTA. After instrumentation roots were randomly divided into four groups, two control groups (positive and negative) and two experimental groups according to root canal sealer: thirty-two teeth of first group were obturated with Apexit Plus and thirty-two teeth of second group were obturated with EndoRez. These groups (1st and 2nd) were further subdivided into two subgroups (a and b) according to the root canal instrumentation technique. After setting time, the roots were incorporated in a leakage model a two chamber method and research on microleakage was conducted for 33 days. The minimum microleakage values were obtained fr...

Journal of Endodontics, 2006

The present study investigated the sealing ability of four root canal sealers at two different thicknesses. There were 82 maxillary incisors roots prepared with Gates Glidden drills up to size 6 and divided into four groups: Pulp Canal Sealer, EndoREZ, Sealapex, and AH Plus. Each group was divided in two subgroups and gutta-percha cylinders 1.5 or 1 mm wide were used, respectively. The roots were mounted in a bacterial leakage model and the system was checked daily during the following 12 wk. Data were analyzed by Log-Rank test and Student t-test. In the thin layer samples, the sealers demonstrated similar results while, in the thick layer samples, AH Plus revealed the best performance. Generally, greater sealer thickness influenced negatively the sealing ability of the root canal filling, except in AH Plus samples. (J Endod 2006;32: 998 -1001)

2019

The aim of the study was to analyse the sealing ability of three root canal sealers: AH Plus, MTA Fillapex and Resilon/RealSeal SE. Methodology: Eighty freshly extracted human permanent mandibular first premolars were decoronated using a diamond disc at a level of 14 mm from the root tip in order to maintain uniformity of specimen length. Cleaning and shaping was done using Hyflex CM rotary instruments. The teeth were randomly divided according to the obturating material into three experimental groups. The coronal part of each root specimen was attached to an apparatus initially proposed by Xu., et al. to assess endodontic microleakage. All specimens were then incubated at 37°C during the observation period. Samples were then analyzed with a glucose kit using colorimetry, and were expressed in mMol/L. Conclusion: None of the sealers were efficient in preventing microleakage within the root canals. Under the experimental conditions, AH Plus had the least amount of endodontic leakage followed by RealSeal SE and MTA Fillapex.