Serum leptin concentrations in chronic hepatitis

BMC Gastroenterology, 2007

Background: The role of leptin in the course of liver disease due to chronic viral hepatitis (CVH) remains controversial. Our aims were to investigate the relationship between serum leptin concentrations and the severity of liver disease in a cohort of subjects with HBeAg negative chronic hepatitis B (CHB) and C (CHC) and to analyze the effect of body composition, the leptin system and insulin resistance together with viral factors on virologic response to antiviral treatment. Methods: We studied 50 (36 men) consecutive patients suffering from biopsy-proven CVH due to HBV (n = 25) or HCV (n = 25) infection. Thirty-two (17 men) healthy volunteers served as controls. Levels of serum leptin and insulin were determined by immunoassays at baseline and at the end of the treatment.

Journal of …, 2000

Background~Aim: Serum levels of leptin, the adipocyte-derived hormone regulating food intake and energy expenditure in mammals, have been found to be increased in cirrhotic patients. The aim of the present study was to investigate leptin serum level in relation to anthropometric features and fiver function in patients with viral chronic hepatitis or fiver cirrhosis. Methods: Serum leptin levels were determined by radioimmunoassay in 30 male and 10 female patients with chronic hepatitis, in 42 male and 10 female patients with fiver cirrhosis, and in four respective control groups. Liver function was evaluated by the monoethylglycinexylidide formation test. Body mass index and body fat mass were estimated by weight, height and skinfold thickness measurements. Results: Compared with controls, absolute serum leptin levels were significantly (p<0.01) lower in chronic hepatitis patients and similar in cirrhotic patients.

American Journal of Gastroenterology, 2000

Leptin is a peptide hormone that mainly regulates food intake and energy expenditure of human body. A close correlation between serum leptin levels and the percentage of body fat stores is well known. Nonalcoholic steatohepatitis (NASH) is a common disorder which causes serum liver enzyme elevation. In this study, the serum leptin levels were investigated in patients with NASH to determine a possible role in the pathogenesis and in patients with chronic viral hepatitis to ascertain the effect of hepatic inflammation on serum leptin level.

Journal of Hepatology, 2000

Background/Aims: Little information is available on the involvement of leptin in clinical conditions associated with malnutrition, such as liver cirrhosis. The behaviour of sermu leptin in patients with different Child-Pugh score, post-hepatitis liver cirrhosis and insulin sensitivity has therefore been investigated and compared with that in alcoholic Child C patients. Methods: Sixty-four patients, aged 51 to 62 years, with different degrees of post-hepatitis cirrhosis or Child C alcoholic cirrhosis were compared with 15 age-matched control subjects. Body composition was estimated by skinfold thickness. Serum leptin, glucose and insulin were assayed. Results: In post-hepatitis patients a significant reduction in leptin levels was observed as the Child-Pugh score worsened (men: 2.9421.61

The Endocrinologist, 2006

In liver cirrhosis, nutritional status is quite impaired, and the current data has shown conflicting results in serum leptin levels of cirrhotic patients. In this study, we aimed to evaluate serum leptin levels in nonalcoholic liver cirrhosis and determine its relationship with anthropometric parameters and liver function. Thirty-three patients (13 males, 20 females) with nonalcoholic cirrhosis and 16 (9 males, 7 females) healthy control subjects were recruited for this study. Serum leptin values were measured in morning samples of both groups by enzyme-linked immunosorbent assay. The mean levels of leptin were not different in nonalcoholic cirrhotic patients when compared with the control group (P ϭ 0.478). Serum leptin values were positively correlated with prothrombin (international normalized ratio) (P ϭ 0.008, r ϭ 0.64) and total bilirubin (P ϭ 0.047, r ϭ 0.488) and were negatively correlated with high-density lipoprotein (P ϭ 0.025, r ϭ 0.666) and albumin (P ϭ 0.009, r ϭ 0.615). No correlation was found between leptin levels and severity of the liver disease. These findings suggest leptin levels do not depend on the degree of liver damage in patients with nonalcoholic cirrhosis of the liver.

There is accumulating evidence that leptin has a pleiotropic role in hematopoiesis, immune response, fibrogenesis, and hepatocarcinogenesis. We investigated the expression of leptin and leptin receptor (OB-R) at the protein level by flow cytometry and also quantified by real-time reverse transcriptase-polymerase chain reaction (RT-PCR) the two major leptin receptor isoforms (OB-Rl, OB-Rs) in peripheral blood mononuclear cells (PBMCs) of patients with hepatitis B (HBV; n ¼ 31), hepatitis C (HCV; n ¼ 34), and nonviral liver disease (n ¼ 25), and healthy controls (n ¼ 36), as well as in liver tissues of HBV (n ¼ 8), HCV (n ¼ 7), and healthy individuals (n ¼ 6). Serum leptin levels were measured in all participants (N ¼ 126). We observed significantly lower OB-Rl and OB-Rs mRNA levels in PBMCs of HBV and HCV patients compared with healthy controls and nonviral liver disease patients (P , 0.05). Flow cytometry analysis confirmed the real-time RT-PCR results. Expression of leptin and OB-Rl was significantly increased in viral hepatitis liver tissues compared with healthy tissues (P , 0.01). OB-Rl mRNA levels were not associated with hepatitis patients' clinical status (inactive, chronic hepatitis, or cirrhosis). We also found decreased serum leptin in HBV and HCV patients compared with healthy individuals and the nonviral liver disease group. Leptin was expressed in 3 of 34 HCV (8.8%) and 19 of 25 (76%) nonviral liver disease patients. Moreover, expression of OB-Rl and OB-Rs were associated when all individuals were grouped together (r ¼ 0.78, P , 0.001). In conclusion, our findings may suggest the involvement of the leptin system in the immunopathology of chronic viral hepatitis.

Journal of thee Medical Sciences (Berkala Ilmu Kedokteran), 2014

Malnutrition is a common condition in liver cirrhotic patients. Leptin regulates body weight physiologically by suppressing appetite and increasing energy expenditure. Leptin is higher in female than male. Studies have shown correlation between leptin with metabolic factors like body mass index (BMI) and lipid profile in cirrhotic patients. This study was conducted to investigate the correlation between serum leptin levels with lipid profile and free fatty acid in male patients with liver cirrhosis. This was a cross sectional study that conducted at Gastroentero-Hepatology Clinic and Internal Ward at Dr. Sardjito General Hospital, Yogyakarta. The inclusion criteria were patients with liver cirrhosis > 18 years old, male, with Child-Pugh classification B and C, and provided informed consent. The exclusion criteria were liver cirrhotic patients with comorbidity chronic kidney disease, chronic heart failure, diabetic, cancer, infection/septic, pregnancy, breast feeding, and steroid use. Data collecting was performed by anamnesis, physical examination, abdominal ultrasonography examination, and blood chemistry test. Pearson test was used to evaluate the correlation between the serum leptin level with the lipid profile and free fatty acid. The results showed that no significant negative correlation was observed between the serum leptin level with the total cholesterol (r=-0.052; p=0.766), high-density lipoprotein/HDL (r=-0.078; p=0.658) and triglyceride (r=-0.170; p=0.328) in male patients with liver cirrhosis. Furthermore, no significant positive correlation was observed between the serum leptin levels with the low-density lipoprotein/LDL (r=-0.013; p= 0.942) and free fatty acid/FFA (r= 0.007; p=0.968). In conclusion, there was no correlation between serum leptin levels with lipid profile and FFA in male patients with liver cirrhosis.

2011

2013

Background: Hepatitis B and C virus is a leading cause of chronic liver disease. Elevated serum alpha� fetoprotein (AFP) has been used as a marker for hepatic regeneration after the destruction of hopatocyte in viral hepatitis. Also Leptin level may be important to determined in patients with chronic hepatitis B and C. Aims: This study aimed to examine the association between serum AFP, serum leptin levels and some other biochemical parameters in patients with chronic liver disease (B and C). Materials and methods: Serum AFP, leptin, total serum protein TSP ,lipid profile, transaminases, alkaline phophatase ALP, total serum bilirubin levels TSB were measured in 61 patients (31 HBV, 30 HCV) and compared with 30 control subject. Results: In comparison to controls, a significant increase in the serum AFP , Leptin, TSP, AST, ALT,ALP & TSB levels (p &lt; 0.00) and a significant decrease in Albumin, cholesterol , Triglyceride, (HDL), (VLDL) & (LDL) levels (p &lt; 0.00) compared to control...

The Southeast Asian journal of tropical medicine and public health, 2000

The weight, height and body mass index (BMI), including waist/hip ratio, serum leptin and hematological parameters of 48 male and 166 female overweight (BMI &gt; or = 25.00) Thai volunteers who came for a physical check-up at the Out-patient Department, General Practice Section, Rajvithi Hospital, Bangkok during the period March-October 1998, were investigated. There were statistically significantly higher levels of serum leptin, mean corpuscular mean corpuscular hemoglobin concentration (MCHC) and mean corpuscular volume (MCV) in the overweight than in the control subjects. The median serum leptin concentration in overweight subjects was 19.6 (2.0-60.0 ng/ml) compared with 9.0 (range 1.0-30.0 ng/ml) in the control subjects (p &lt; 0.001). The medians of leptin in overweight and obese males were significantly higher than those of overweight and obese females. 66.7% (32 out of 48) of overweight and obese males were found to have elevated leptin levels, while 87.3% (145 out of 166) we...