Skin Structure and Wound Healing Phases

Background and aim: The effect of herbal medicine with antitoxidant activity on burn wound healing has been proven in various studies. Portulaca oleracea L (Purslane) is a rich source of antioxidants and fatty acids. This study was carried out to compare the effect of purslane and silver on the second-degree burn wound healing in Balb/c mice. Methods: In this preclinical study, burn wounds were made with a hot plate on the back of 85 Balb/c mice. Four groups of mice were treated topically for 21 days by vaseline, silver salfadiazin, 1% and 10% purslane. Wound size on days 0, 7, 14 and 21 were measured. Data were analyzed using Kruskal-Wallis and test. Results: Results showed that the recovery was observed in silver group, 10% purslane, 1% purslane and vaseline, respectively. There was no significant difference in burn wound healing between silver and 10% purslane groups on days 14 and 21 (P>0.05), but significant difference between vaseline and 10% purslane groups on days 14 and 21 (P<0.05). Conclusion: Regarding to the finding of this study, extracts of 10% purslane expedites the wound healing process in mice. Therefor, it might be beneficial in patients with burn wound.

Journal of Shahrekord University of Medical Sciences, 2011

Background and aim: Rat and human skin tumors have high similarity in physiology and show comparable biochemical responsiveness. In this study we have recorded numerical and structural chromosomal changes in metaphases chromosomes in induced-breast cancer in rats. Methods: In this experimental study, a carcinogenic substance (7,12-Dimethylbenz[α] anthracene, DMBA) (2.5 mg) was subcutaneously injected to SD rats. Since tumors were appeared in effected rats, they became subjects for histopathology and imunohistochemistry studies as well as cell culture, metaphase chromosomal preparations and finally G-banding staining. Results: The data showed some un-randomly numerical and structural changes in different number of chromosomes. We found a gain in chromosomes number 8, 9, 10, 18 and a loss of numerical chromosomal changes in chromosomes number 4, 6, 12, 16 ِ ِ ِ ِ ِ Deletion was seen in chromosomes number 1, 4, 5, 6, 17 and marker chromosomes in diploid and triploid model of analyzed chromosomal sets. Conclusion: According to our reported chromosomal changes and also published articles, we predict that SRD5A2, BCAM, SIRT2, AKT2, MLANA, RHOB, CANX, TERT are probably involved in development of skin cancer. In addition, we recommend that the genes located in these chromosomal segments, which have been reported in these studies, should be subjected to more investigations.

Koomesh, 2011

Materials and Methods: A retrospective multistage study in two phases (the first stage a historical cohort and the second stage a case control) was carried out over a 6 year period from 21 st March 2002 to 20 th Feb 2008 in Mahdieh Tertiary Care Hospital. In the first ...

Journal of Babol University of Medical Sciences, 2005

DOAJ (DOAJ: Directory of Open Access Journals), 2021

Research in Medicine, 2009

Journal of Mazandaran University of Medical Sciences, 2014

Background and purpose: To assess the effect of equilibration time on the vitrified-warmed two cell mouse embryos. Material and Methods: Two cell embryos were obtained from oviducts of super ovulated female NMRI mice. The embryos were randomly allocated in four groups and vitrified using cryotop and two-step mediaprotocol. The experimental groups were: 1) embryos were equilibrated in vitrification solution (v1) (7.5% EG, 7.5% DMSO, 20% BSA, Ham's F10) for 5 min (n=122), 2) 10 min (n=102), 3), 15 min (n=105), 4), 20 min (n=140). After two weeks, the vitrified embryos were thawed. The survived embryos were cultured in Ham, s F10 medium for 72 hours and the cleavage, blastocyst formation and hatching rates were assessed. The results demonstrated that survival, cleavage, blastocyst formation and hatching rates did not show any significant differences among the embryos in different groups which exposed in first vitrification solution for periods of 5 to 20 minutes. The range of 5 to 20 minutes for equilibration of mouse embryos in the first vitrification solution can be used in the two-step media plus cryotop method for embryo vitrification.

Background and purpose: Perineal lacerations are common complications of normal vaginal delivery which influence maternal health and quality of life of mothers. This study aimed at comparing perineal protection using hands on and hands off techniques on perineal lacerations during delivery. This clinical trial included 600 nulliparous women who were randomly divided into hands on and hands off groups (n=300 per group). In hands on technique when the head was crowning, a midwife placed her index, middle, ring and little fingers on the infant's occiput, simultaneously she placed her other hand (index finger and thumb) in a "u" shape exerting pressure on the posterior region of the perineum. In hands off technique during expulsive period the midwife's conduct was expectant, as she only observed the successive movement of external rotation, delivery of shoulder and remainder of the body. The data was analyzed using chi -square and independent T-Test in Results: The rate of Perineal lacerations was not different between the two groups (P=0.7). In both groups first degree lacerations were more dominant. Three degree lacerations (P=0.01) and episiotomy (P=0.003) were significantly lower in hands off group but the rate of periurethral lacerations was significantly higher in hands-off group (P=0.01). Second stage of labor and neonatal outcomes were similar in both groups. In present study the use of hands off technique reduced the rate of episiotomy and three degree lacerations of birth canal, therefore, it could be recommended as a safe method during labor..