Platlet-Rich Plasma – Review of Literature

Cell and Tissue Banking

Rise in the incidences of chronic degenerative diseases with aging makes wound care a socioeconomic burden and unceasingly necessitates a novel, economical, and efficient wound healing treatment. Platelets have a crucial role in hemostasis and thrombosis by modulating distinct mechanistic phases of wound healing, such as promoting and stabilizing the clot. Platelet-rich plasma (PRP) contains a high concentration of platelets than naïve plasma and has an autologous origin with no immunogenic adverse reactions. As a consequence, PRP has gained significant attention as a therapeutic to augment the healing process. Since the past few decades, a robust volume of research and clinical trials have been performed to exploit extensive role of PRP in wound healing/tissue regeneration. Despite these rigorous studies and their application in diversified medical fields, efficacy of PRP-based therapies is continuously questioned owing to the paucity of large samplesizes, controlled clinical trials, and standard protocols. This review systematically delineates the process of wound healing and involvement of platelets in tissue repair mechanisms. Additionally, emphasis is laid on PRP, its preparation methods, handling,

IOSR Journals , 2019

Introduction: wound management is a challenging task to the surgeon and a debilitating issue to the patients. The general treatment including the expert care in dressings, meticulous use of antibiotics, Negative pressure vacuum therapy dressings, surgical interventions and reconstructions using a lot of resources, expenses and expert hands. But the results are still unpredictable and unassured. Platelet-rich plasma (PRP) has a numerous number of growth factors, chemokines, cytokines and many other proteins which have a numerous number of properties out of which one is its role in wound healing. Materials and Methods: The PRP therapy consists of self-activated autologous platelet-rich plasma derived from centrifugation of whole blood by Double centrifugation method. It is given as a local subcutaneous infiltrate along the wound margins on every fourth day till the wound is completely healed with regeneration of skin or red healthy granulation tissue. The study consisted of 30 patients, ages ranging from 20 to 60, both males and femaleswho were studied from January 2017 to december 2018.Hb per cent below 9 was a contraindication. The wounds consisted of both acute and chronic, including compound fractures, infected wounds, non-healing ulcers. Results: 28 wounds were healed by this technique which did not require any further procedures and two of them required split skin grafting. The average number of sittings required were seven. Antibiotic cultures showed no bacterial growth during the course of treatment. Conclusion: platelet-rich therapy is a novel mode of treating wounds by their growth factor properties and antibacterial properties giving satisfying results without wasting resources nor need of any other complex and expert techniques in wound

USE OF PLATELET-RICH PLASMA FOR DERMATOLOGICAL WOUND HEALING: A LITERATURE REVIEW (Atena Editora), 2024

This article discusses the use of platelet-rich plasma (PRP) as a promising tool in dermatology to accelerate the healing of skin wounds. PRP is a blood derivative that contains high concentrations of platelets and growth factors, which play a crucial role in tissue regeneration. It stimulates angiogenesis, fibroblast proliferation and collagen synthesis, significantly accelerating the healing process. The article also highlights the effectiveness of PRP in chronic wounds, which are often slow to heal, emphasizing its relevance as an innovative treatment in the aesthetic field.

2017

Background: Wound healing follows a predictable course of overlapping phases, which involve cellular and biochemical events: (a) hemostasis and inflammation, (b) proliferation and (c) maturation and remodeling. Platelet Rich Plasma (PRP) is obtained by the blood from the patient being concentrated using different techniques. In the light of this basic information, platelet rich plasma (PRP) is used in many areas, especially orthopedic, oral and maxillofacial, dental, ophthalmic, plastic and reconstructive surgeries. In this experimental study performed on rats, the effects of PRP in wound healing were investigated. Methods: The study included 24 male Wistar Albino rats. The animals were divided into three groups, each consisting of 8 animals. Any treatment was not given for defects created in Group 1 and it formed the control group. PRP was applied topically on lesion in Group 2. PRP was injected on the lesion in Group 3. Results: Angiogenesis increased with the topical application,...

Acta Bio Medica : Atenei Parmensis, 2017

Platelet Rich Plasma (PRP) represents a relatively new approach in regenerative medicine. Its use is increasing in the treatment of a variety of orthopedic conditions, such as the management of acute wounds, chronic non-healing lesions, acceleration of bone formation, tendinopathy and chondropathy. The Orthopaedic Unit at Oglio Po Hospital uses PRP Gel in wound care since 2008. More than 70 patients have been treated with this procedure. The aim of this study is to show our patients outcome. We present 3 cases of severe traumatic wound treated with our PRP protocol. The patients had complete healing of the lesion with good aesthetic results.

Platelet-rich plasma (PRP) is an autologous product derived from whole blood through the process of gradient density centrifugation. Autologous PRP has been shown to be safe and effective in promoting the natural processes of wound healing, soft tissue reconstruction, and bone reconstruction and augmentation. The potential value of PRP lies in its ability to incorporate high concentrations of platelet-derived growth factors, as well as fi brin, into the graft mixture. Recently published studies have demonstrated benefi cial results with PRP used in a broad range of clinical healing applications. PRP has been shown to increase the rate of bone maturation and to improve bone density when added to small bony defects, or to larger defects in combination with grafting material. Moreover, PRP can be exogenously applied to soft tissues to promote wound healing and tissue sealing. In patients undergoing certain surgical procedures, perioperative use of PRP may decrease the length of hospitalization and the need for allogeneic blood products. PRP is a promising biotechnology that is fueling growing interest in tissue engineering and cellular therapeutics.

International Journal of Contemporary Medical Research [IJCMR], 2018

Introduction: Chronic leg ulcers pose a formidable challenge to the surgeon necessitating various treatment modalities. Due to recurrence in wounds with conventional therapy, newer modalities of treatment with PRP showed promising results in various branches of medicine like plastic and reconstructive surgery, orthopedics, and dermatology, cardiovascular and thoracic surgery. Study aimed to record the effect of platelet rich plasma in the treatment of chronic ulcers particularly venous and diabetic leg ulcers. Material and methods: A prospective study of 10 cases of chronic leg ulcers. Chronic leg diabetic ulcers, venous foot ulcers of more than 3 months duration both males and females with normal platelet count and activity are selected. Age group between 18ys and 60 yrs with ulcers between size varying 2x2cm 2 to 10 x10 cm2 were included. Results: Wounds were measured on day O along their greater dimensions and were recorded, which ranged from 3x3 cm2 to 10x8. At the end of 2nd week, ulcer size had reduced by an average of 21.87% (range 0%-60%) in 10 wounds. Healing of the ulcers with full re-epithelialization was seen in 4 cases (40%). Of the remaining ulcers, 6 (60%) were taken up for skin grafting once the ulcer has granulated well. In these cases of skin grafting, 5 ulcers have healed completely with 1 case of graft failure Time taken for the wounds to heal completely by re-epithelization ranged from 6 weeks (1 venous ulcer) to 16 weeks (1 diabetic foot ulcer). Of the ulcers (n=6, 60%) which underwent skin grafting, 5 (83.33%),healed completely, with 1 case of graft fail. Conclusion: The use of platelet-rich plasma can be an option when treating recalcitrant wounds of differing etiologies.

Journal of IMAB - Annual Proceeding (Scientific Papers), 2016

OBJECTIVE: To show analysis of the results of applying the method platelet-rich plasma (PRP) for the treatment of problematic skin wounds. The paper's objective is to prove its reliability and relevance, to evaluate its efficiency and applicability to Bulgarian patients. MATERIAL AND METHODS: Out of a total of 154 hospitalized patients with problematic skin wounds 83 have been treated with platelet-rich plasma, comprising the Experimental group (EG), and 71 patients with similar wounds have been treated by using traditional methods for the respective pathology at the Department of Orthopedics and Traumatology, Vessel and Plastic Surgery Department, comprising the Control Group (CG). Data on the condition of monitored symptoms of researched cases was collected for the research. A variable is defined for every symptom and its value and weight is correspondingly introduced. Scores introduced by Cancela AM are used for the assessment of the respective wound. Each of these scores is used for assessing specific wound parameters: Total Wound Score (TWS); total anatomic score (TAS) of wound and Total Score of Wound data (TSWD). RESULTS: Analysis of variance was performed to achieve the objectives and solve the research tasks; variables containing information on initial and final data from each series of experiments have been analyzed in order to determine basic numerical characteristics of variables, and by comparative analysis to check how they tend to vary in experimental (EG) and control (CG) group. Statistical survey shows that the platelet-rich plasma method gives significant results when treating problematic skin wounds leading to full recovery as compared to traditional methods typical for the pathology. The graphical interpretation allows to identify forecast relations between assessed aspects of problematic wounds and weeks of treatment. CONCLUSION: By analyzing the results of our study we can conclude that the use of platelet-rich plasma to treat problematic skin wounds is a safe and effective treatment method. It is not universal for every wound, but follows the principles of biological wound treatment and leads to full recovery of high percentage of problematic skin wounds. We support proponents of the application of platelet-rich plasma to treat problematic skin wounds.

Annals of burns and fire disasters, 2012

Platelet-rich plasma (PRP) is a volume of plasma fraction of autologous blood having platelet concentrations above baseline whole-blood values due to processing and concentration. PRP is used in various surgical fields to enhance soft-tissue and bone healing by delivering supra-physiological concentrations of autologous platelets at the site of tissue damage. These preparations may provide a good cellular source of various growth factors and cytokines, and modulate tissue response to injury. Common clinically available materials for blood preparations combined with a two-step centrifugation protocol at 280g each, to ensure cellular component integrity, provided platelet preparations which were concentrated 2-3 fold over total blood values. Costs were shown to be lower than those of other methods which require specific equipment and high-cost disposables, while safety and traceability can be increased. PRP can be used for the treatment of wounds of all types including burns and also ...

Plastic and reconstructive …, 2006

Learning Objectives: After studying this article, the reader should be able to: 1. Define the role of platelets in hemostasis and wound healing. 2. Describe the technologies for platelet concentration and application. 3. Characterize the platelet concentration and growth factor components of platelet-rich plasma. 4. List the potential applications of platelet-rich plasma in plastic surgery and how it may be applied intraoperatively. 5. Discuss the limitations of the use of platelet-rich plasma and its potential complications.