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Microscopy Research and Technique
This article presents a review after an exhaustive search that yielded 23 works carried out in the last decade for the availability of optical microscopes with open hardware as a low-cost alternative to commercial systems. These works were developed with the aim of covering needs within several areas such as: Bio Sciences research in institutions with limited resources, diagnosis of diseases and health screenings in large populations in developing countries, and training in educational contexts with a need for high availability of equipment and low replacement cost. The analysis of the selected works allows us to classify the analyzed solutions into two main categories, for which their essential characteristics are enumerated: portable field microscopes and multipurpose automated microscopes. Moreover, this work includes a discussion on the degree of maturity of the solutions in terms of the adoption of practices aligned with the development of Open Science. Research Highlights Concise review on low-cost microscopes for developing Open Science, exposing the role of smartphone-based microscopy. The work classifies microscopes in two main categories: (1) portable field microscopes, and (2) multipurpose automated microscopes.
Journal of Microscopy and Ultrastructure, 2020
A light microscope was upgraded to a telemedicine-ready microscope with nominal cost and moderate effort. It can also be used in medical teachings as it can project real-time images of a slide under the microscope. As it is equipped with LEDs, powered by the same smartphone, it can be operated without daylight or during a power outage.
PROCEEDINGS OF THE III INTERNATIONAL CONFERENCE ON ADVANCED TECHNOLOGIES IN MATERIALS SCIENCE, MECHANICAL AND AUTOMATION ENGINEERING: MIP: Engineering-III – 2021, 2021
One of the most widely used biological and medical instruments are the microscopes. Many new modalities have been developed by constant advancement in the field of microscopy, but their relative sizes and their complexity, and their costs often hinder the usefulness of these instruments in the wider general community and many field environments. In this research, we used a 3D printer and the smartphone camera to design and construct a microscopic prototype to create a relatively low-cost, solid structure and to get pictures that are economically viable and are necessary for recording, analysis, education, and publication to acquire and distribute digital photomicrographs. The two-dimensional program, computer-aided design (Auto CAD) and the three-dimensional program (3D MAX) for 3D printed parts have been used to model and print the necessary components for the microscope prototype. The optical elements include a smartphone camera, an eyepiece, and an objective lens. The use of a traditional eyepiece facilitates device two-way compatibility of smartphones and software with a conventional microscope. The prototype microscope examined several specimens of animal tissue such as skin, follicle hair, and connective tissue. The photos were really accurate, clear, and magnified enough to see the tiny details of the biological cells and tissues. The resulting magnification was comparable to 10x of conventional microscope.
2019
Optical microscopes are an essential tool for both the detection of disease in clinics, and for scientific analysis. However, in much of the world access to high-performance microscopy is limited by both the upfront cost and maintenance cost of the equipment. Here we present an open-source, 3D-printed, and fully-automated laboratory microscope, with motorised sample positioning and focus control. The microscope is highly customisable, with a number of options readily available including trans- and epi-illumination, polarisation contrast imaging, and epi-florescence imaging. The OpenFlexure Microscope has been designed to enable low-volume manufacturing and maintenance by local personnel, vastly increasing accessibility. We have produced over 100 microscopes in Tanzania and Kenya for educational, scientific, and clinical applications, demonstrating that local manufacturing can be a viable alternative to international supply chains that can often be costly, slow, and unreliable.
Microscopy Today
The smartphone microscope, or mobile microscope, is an interesting tool that is attracting attention in various fields. We report a model of mobile microscope that extends from the Leeuwenhoek-type of single microscope with a simple lens. The new mobile microscope is called the "L-eye mobile microscope" and is compact and easy to handle. The L-eye mobile microscope may become a hobby tool in everyday life for people of all ages but may also be a tool suitable in the education of medical or paramedical students. To test its potential as an educational tool, its performance was examined using samples such as permanent specimens of tissue sections from several organs used in histology and a standard target for a microscopy resolution test. As a control, we used a conventional education-use microscope that is mounted with a smartphone for image capture. From the performance comparison of the two kinds of microscope systems, the L-eye mobile microscope could take images with almost the same quality as those obtained with the conventional microscope at moderate magnifications. This result suggests the usefulness of the L-eye mobile microscope for education.
Optics Education and Outreach V, 2018
Microscopes play a central role in hands-on science exploration and communication. All too often, however, students do not have a good understanding of the optical principles that govern microscopy and lack access to instruments that could help them build that understanding. Here we present an open-source Do-It-Yourself (DIY) microscope kit developed by scientists and students at BioBus, a nonprofit organization for science outreach and education based in New York City (www.biobus.org). The DIY microscope uses 3D printing to make highly adaptable optical technology readily available to educators at a low cost. Its modular configuration makes it the perfect tool to teach optical design to students of all ages. At BioBus, Inc., over 230 school-age students and teachers learned basic optics and microscope building with the DIY microscope at our community and mobile laboratories. We further present examples of how the DIY microscope was used as a platform for student-driven projects, expanding the original design to include advanced optical features such as fluorescence and infrared imaging. The images, acquired with a low cost camera, were comparable in quality to those taken using professional grade laboratory microscopes. The use of the DIY microscope is not limited to applications in physical sciences, but can also be used as an interdisciplinary teaching tool. As an example, we showed how it was configured into a functional model of the eye, to explain the physics of vision and the pathophysiology of eye disorders, such as far and short-sightedness, and age-related macular degeneration.
Open-source technology not only has facilitated the expansion of the greater research community, but by lowering costs it has encouraged innovation and customizable design. The field of automated microscopy has continued to be a challenge in accessibility due the expense and inflexible, non-interchangeable stages. This paper presents a low-cost, open source microscope 3-D stage. A RepRap 3-D printer was converted to an optical microscope equipped with a customized, 3-D printed holder for a USB microscope. Precision measurements were determined to have an average error of 10 μm at the maximum speed and 27 μm at the minimum recorded speed. Accuracy tests yielded an error of 0.15%. The machine is a true 3-D stage and thus able to operate with USB microscopes or conventional desktop microscopes. It is larger than all commercial alternatives, and is thus capable of high depth images over unprecedented areas and complex geometries. The repeatibility is below 2-D microscope stages, but testing shows that it is adequate for the majority of scientific applications. The open source microscope stage costs less than 3% to 9% of the closest proprietary commercial stages. This extreme affordability vastly improves accessibility for 3-D microscopy throughout the world.
ABSTRACT We present a lensfree digital microscopy platform implemented on a cell-phone. It operates based on digital in-line holography and provides a compact and light-weight alternative to conventional microscopes, such that the cell-phone is modified with an inexpensive attachment weighing only~ 38 grams. This lensfree cell-phone microscope captures holographic images of the objects which are then rapidly processed by a custom-developed reconstruction algorithm to provide microscopic images of the sample.
Journal of Biological Education
ABSTRACT A Foldscope is an ultra-affordable paper microscope with a magnification up to 2000X and 2 micron resolution. It is economic, durable and easy to handle tool for scientific studies. Many govt. schools of Kamrup district, Assam found it difficult to use microscope in their regular practical classes for its complexity and high cost. For visualization of specimen under Foldscope, light source used is either natural light (during day time) or artificial light source operating on batteries. Being extremely light weight and portable, it could be easily carried along to field for various studies. In the present study, practical sessions and awareness on this instrument was conducted in the govt. and private schools of Kamrup (M), Assam. Students and school authority’s feedback were collected for analyzing the scope of Foldscope in future use as a part of their syllabus. The hands on training of the instrument helped the student’s to understand microscopic world of biology. Hence, it could be concluded that Foldscope could be incorporated in every school’s syllabus for better understanding of biology by reducing school experimental expenses and overcoming the facility constraints.
PLoS ONE, 2009
Light microscopy provides a simple, cost-effective, and vital method for the diagnosis and screening of hematologic and infectious diseases. In many regions of the world, however, the required equipment is either unavailable or insufficiently portable, and operators may not possess adequate training to make full use of the images obtained. Counterintuitively, these same regions are often well served by mobile phone networks, suggesting the possibility of leveraging portable, camera-enabled mobile phones for diagnostic imaging and telemedicine. Toward this end we have built a mobile phonemounted light microscope and demonstrated its potential for clinical use by imaging P. falciparum-infected and sickle red blood cells in brightfield and M. tuberculosis-infected sputum samples in fluorescence with LED excitation. In all cases resolution exceeded that necessary to detect blood cell and microorganism morphology, and with the tuberculosis samples we took further advantage of the digitized images to demonstrate automated bacillus counting via image analysis software. We expect such a telemedicine system for global healthcare via mobile phone -offering inexpensive brightfield and fluorescence microscopy integrated with automated image analysis -to provide an important tool for disease diagnosis and screening, particularly in the developing world and rural areas where laboratory facilities are scarce but mobile phone infrastructure is extensive.
Researchgate, 2020
A. Microscope (Compound or stereo): The primary need. B. Camera (on head or eyepiece of microscope): To capture the microorganism. C. Computer or laptop: To observe in big screen and store.
Revista de Monografias Ambientais, 2020
The teaching of life sciences in elementary school faces a major problem: the lack of materials for laboratory practice. This lack of equipment results in many cases in the transformation of the class into theoretical information routines, discouraging the student to care for nature. The sense of admiration that should be worked with at this level of education is now neglected and the trainee is enchanted with technology and not dedicated to caring for nature. Taking the student to a set of activities that return his taste for nature, especially with the use of alternative microscopy was the objective of the present work. Using alternative materials, especially magnifying glasses from various equipment, three alternative microscopes were assembled, which were tested and worked with teachers of a school to verify their potential use in class. The assembled equipment proved to be efficient for microscopic studies, especially to arouse the student's interest in the subject, with the possibility of even having his own equipment at home and continuing to develop a sense of charm for nature. The increase achieved was 300 times. Possible activity kits were assembled, which are presented in the form of routine work with a microscopy laboratory. The evaluation of the equipment allows the possibility of working disciplines such as biology, chemistry, physics and mathematics in an interdisciplinary manner.
9th International Conference on Remote Engineering and Virtual Instrumentation (REV), 2012, 2012
We have engineered an optical microscope that can be operated via the internet using most of the internet and networking capable devices including laptops and desktop computers. The system offers a user the ability to conduct visual inspection of samples, microelectronic devices and manipulation experiments in the field of biology. The remote controlled microscope is programmed to perform acquisition of images. The instrument is currently being used to perform thin film characterization experiments in microelectronics laboratory. Such a system may find a wide range of new applications for science experiments.
PLoS ONE, 2011
Background: The emerging market of mobile phone technology and its use in the health sector is rapidly expanding and connecting even the most remote areas of world. Distributing diagnostic images over the mobile network for knowledge sharing, feedback or quality control is a logical innovation. Objective: To determine the feasibility of using mobile phones for capturing microscopy images and transferring these to a central database for assessment, feedback and educational purposes. Methods: A feasibility study was carried out in Uganda. Images of microscopy samples were taken using a prototype connector that could fix a variety of mobile phones to a microscope. An Information Technology (IT) platform was set up for data transfer from a mobile phone to a website, including feedback by text messaging to the end user. Results: Clear images were captured using mobile phone cameras of 2 megapixels (MP) up to 5MP. Images were sent by mobile Internet to a website where they were visualized and feedback could be provided to the sender by means of text message. Conclusion: The process of capturing microscopy images on mobile phones, relaying them to a central review website and feeding back to the sender is feasible and of potential benefit in resource poor settings. Even though the system needs further optimization, it became evident from discussions with stakeholders that there is a demand for this type of technology.
Optics in the Life Sciences, 2011
In this paper we report the development of two attachments to a commercial cell phone that transform the phone's integrated lens and image sensor into a 3506 microscope and visible-light spectrometer. The microscope is capable of transmission and polarized microscopy modes and is shown to have 1.5 micron resolution and a usable field-of-view of *1506150 mm with no image processing, and approximately 3506350 mm when post-processing is applied. The spectrometer has a 300 nm bandwidth with a limiting spectral resolution of close to 5 nm. We show applications of the devices to medically relevant problems. In the case of the microscope, we image both stained and unstained blood-smears showing the ability to acquire images of similar quality to commercial microscope platforms, thus allowing diagnosis of clinical pathologies. With the spectrometer we demonstrate acquisition of a white-light transmission spectrum through diffuse tissue as well as the acquisition of a fluorescence spectrum. We also envision the devices to have immediate relevance in the educational field.
Proceedings of the 38th Annual Hawaii International Conference on System Sciences
We evaluated the diagnostic accuracy of a virtual microscopy setup using surgical pathology specimens commonly encountered in a university hospital setting. The high quality images, Internet sharing and collaborative capability, interactivity, and ease of use suggested to us that this might have applications in countries with developing economies. We discuss the development process and its potential applications in medical education and telemedicine in countries with developing economies.
2012
Abstract Within the last few years, cellphone subscriptions have widely spread and now cover even the remotest parts of the planet. Adequate access to healthcare, however, is not widely available, especially in developing countries. We propose a new approach to converting cellphones into low-cost scientific devices for microscopy. Cellphone microscopes have the potential to revolutionize health-related screening and analysis for a variety of applications, including blood and water tests.
Tenth International Topical Meeting on Education and Training in Optics and Photonics, 2015
In schools, scientific education with an optical microscope is popularly used. However, scanning apparatus for the microscope is very expensive such that the price is several times higher than the microscope itself. In order to activate children's interest in science, a low-price scanning and imaging function unit compatible to conventional optical microscopes used in schools was designed and manufactured using a personal computer (PC) used in all elementally and middle school education. The designing of imaging apparatus includes two choices: (i) using imaging device (reflection-type), or (ii) using photo-sensor and scanning device (transmission-type). In this paper, the latter method is adopted, considering the educational effect using "Lambert-Beer's law". This apparatus measures optical transmittance of modulated visible light with a photo-detector, and uses audio-input unit of PC as an A/D converter. Scanning unit with a pair of pulse motor drives was also used. Control software was built on Knoppix (an operating system based on freeware Linux), however it is very easy to rewrite to Windows application. By these reasons, this apparatus is low-price (less than microscope price) so that it is one of the best candidates for science education application in schools. As a biological specimen, a wing of spider wasp (Pompilidae) was used. Measured region was 10mm×10mm and the resolution was 100×100 pixels. The photograph of original specimen and the obtained image were shown in Figures (a) and (b), respectively. The obtained image showed a well-resolved detailed structure of the wing. Scanning was done by an external scanning apparatus. However, feeding of scanning pulses through printer port to stepping motor will be available based on the same method.
—the use of high accuracy positioning systems provides endless possibilities for the development of remote laboratories. The remote laboratory presented in this paper allows full control of a microscope over a surface by the use of a Cartesian positioning system. The arrangement of multiple samples over the accessible surface by the lens so far as the provision of a rich graphical user interface will allow secondary school students to carry multiple experiments in biology, physics and chemistry through internet.
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