ラット上顎臼歯におけるBMP-石灰藻由来ハイドロキシアパタイト複合体の修復象牙質誘導

Japanese Journal of Oral & Maxillofacial Surgery, 1987

We experienced an advanced oral cancer with a total resection of upper lip and partial maxillectomy, including nasal floor. Local flap was found to be inadequate to cover the large defect of lip and maxilla. The free forearm flap which contained brachioradialis muscle was used,and this offered an ideal solution for improving thickness and muscular function in the reconstructed lip. Postoperative condition of the flap was excellent, and healing of donor site was satisfactory. The follow up study of nearly 25 months found no recurrence or metastasis.

日本プランクトン学会報, 2010

B u l l . P l a n k t o n S o c . ] a p a n 5 7 ( 2 ) : 7 3 -7 8 ， 2 0 1 0 日本プランクトン 学会報 。 T h eP l a n k t o n S o c i e t y o f ] a p a n 2 0 1 0 藍藻 M i c r o c y s t i sS p p .に対するアカシア属樹木抽出物質の増殖抑制効果 3 ) 滋賀県立大学環境科学部，干 5 2 2 -8 5 3 3滋賀県彦根市八坂町 2 5 0 0 I n h i b i t o r y e 町e c t so f A c a c i a e x t r a c t o n g r o w t h o f b l u e -g r e e n a l g a e M i c r o c y s t i s r s i t y 0 1 S h i g a P r e l e c t u r e ， S c h o o l 0 1 E n v i r o n m e n t ， α l S c i e n c e s ， 2 5 0 0 H a s s a k a -c h o ， H i k o n e ， S h i g a 5 2 2 -8 5 3 3 ， ] a p a n * C o r r e s ρ o n d i n g a u t h o r . E -m a i l : b α n @ s e s . u s p . α c . j j う A b s t r a c t E 妊e c t so f A c a c i a e x t r a c t ( A M E ) f r o m A c a c i αd e c u r r e n s Wi 1 1 d e n o w o n g r o w t h o f t w o s p e c i e s o f c y a n o b a c t e r i a ， M i c r o c y s t i s i c h t h y o b l α b e K u t z i n g ( M 9 5 ) a n d M i c r o c y s t i s a e r u g i n o s α ( K u t z i n g ) K u t z i n g ( N E I S 2 9 8 ) w e r e e x a m i n e d . AME s h o w e d a n i n h i b i t o r y e 妊e c to n c y a n o b a c t e r i a l g r o w t h i n b o t h s p e c i e s a t 6 mg L -1 ， a n d i n d u c e d n e g a t i v e g r o w t h a t 1 2 mg L -1 . On t h e c o n t r a r y ， t h e r e w a s n o e 百e c to f AME o n t h e g r o w t h o f g r e e n a l g a ， C h l a m y d o m o n a s r e i n h a r d t i i D a n g e a r d ( I A M C -9 ) e v e n a t 1 2 mg L -1 . A n a l y s i s u s i n g s i l i c a g e l t h i n -l a y e r c h r o m a t o g r a p h y s e p a r a t e d f o u r b a n d s ， b a n d 1 -4 ， f r o m t h e AME w i t h a m o b i l e p h a s e s o l v e n t ( l -B u t a n o l : A c e t i c a c i d : H 2 0=4: 1・ 2 ( v j v j v ) ) ， a n d b a n d 2 i n d u c e d t h e m o s t i n h i b i t o r y e f f e c t o n b o t h M . i c h t h y o b l a b e a n d M α e r u g i n o s a g r o w t h . L i q u i d c h r o m a t o g r a p h y -m a s s s p e c t r o m e t r y s u g g e s t e d t h a t b a n d 2 i n c l u d e d g a l l i c a c i d ， e p i c a t e c h i n ， a n d o l i g o m e r ( d i m e r ， t r i m e r ， t e t r a m e r a n d p e n t a m e r ) c a t e c h i n s . T h e s e r e s u l t s s u g g e s t e d t h a t t h e c a t e c h i n s w e r e t h e m a i n i n h i b i t o r s i n b a n d 2 s e p a r a t e d f r o m AME o n c y a n o b a c e r i a l g r o w t h . K e y w o r d s : A c a c i a e x t r a c t ， M i c r o c y s t i s ， i n h i b i t o r y e f f e c t s ， o l i g o m e r c a t e c h i n s ， g a l l i c a c i d は じ め に これまでにアオコ(すなわち，藍藻フールーム)の抑制 については，湖水の曝気などによる物理的方法

The Journal of the Geological Society of Japan, 2006

Journal of Hard Tissue Biology, 2005

The ability of osteoblasts to form bone greatly diminishes with aging, which may be due to the rise of oxygen-derived free radical formation. However, changes that occur in the mineral crystallinity of bone nodules formed by osteoblasts under conditions of oxidative stress have not been sufficiently clarified. The aim of this study was to elucidate the effects of oxidative stress on osteogenesis. We investigated bone nodule formation in an osteoblast-like cell line, MC3T3-E1, using von Kossa staining and micro-X-ray diffraction (micro-XRD). For the micro-XRD examinations, the bone nodules were analyzed after removal from the culture plate and placed on a slide glass or a silver membrane filter. We found that nodule formation was lower on day 30 following treatment with H 2 O 2 and mineral crystallinity in H 2 O 2-treated cells was also lower than in the controls. These results suggest that osteoblasts damaged by oxygen free radicals produced during the aging process show a decline in their bone nodule formation ability.

Journal of Hard Tissue Biology, 2007

The purpose of this study was to evaluate synthetic calcium phosphates and animal bones using Raman spectroscopy, and explore the possibility of its application in characterizing newly formed bone around implants. Synthetic calcium phosphates (monobasic calcium phosphate, dibasic calcium phosphate and tribasic calcium phosphate), hydroxyapatite and rabbit bones with and without implants were analyzed in this study. Slight differences in the Raman bands among the 3 types of synthetic calcium phosphate and hydroxyapatite were observed. Furthermore, a 3 cm-1 difference was noted in the bands of the main PO 4 3in rabbit's bone formed around the implant compared to the existing bone, suggesting a difference in the molecular structure between the existing and newly formed bone.