Nafion/copper particulates for H2O2 sensing

Indian journal of chemistry. Section A, …, 2009

Abstract: Nafion/copper particulates chemically modified glassy carbon electrode has been prepared by in situ electrochemical deposition. The electrode is found to to be successful for selective amperometric sensing of hydrogen peroxide at-0.2 V vs Ag/AgCl at physiological ...

Journal of the Brazilian Chemical Society, 2016

A nanosensor was developed for hydrogen peroxide determination based on nafion/graphene oxide/silver nanoparticles/tertiary butylhydroquinone (TBHQ) modified glassy carbon electrode (N-GO/AgNPs/TBHQ/GCE). Cyclic voltammetry was used to investigate the electrochemical behavior of this modified electrode and differential pulse voltammetry was used for the reduction of H 2 O 2. The limit of detection was 0.46 µmol L-1 and three linear calibration ranges were obtained for H 2 O 2 determination from 1.52-9.79 µmol L-1 for first linear segment, 9.79-231.0 µmol L-1 for second linear segment and 231.0-8330.0 µmol L-1 for third linear segment. Finally, the reliability of the nanosensor was confirmed in the real sample analysis in different beverages with satisfactory results.

Talanta, 2013

Electrochemical detection of H 2 O 2 was investigated on a Nafion/exfoliated graphene oxide/Co 3 O 4 nanocomposite (Nafion/EGO/Co 3 O 4) coated glassy carbon electrode. The morphological characterization was examined by scanning electron microscopy, X-ray diffraction, and electrochemical impedance spectroscopy. The modified electrode showed well defined and stable redox couples signal in both alkaline and natural aqueous solutions with excellent electrocatalytic activity for oxidation of hydrogen peroxide. The response of the modified electrode to H 2 O 2 was examined using amperometry (at 0.76 V vs. Ag/AgCl reference electrode) in a phosphate buffer solution (pH 7.4). The detection limit was 0.3 mmol L À 1 with a linearity of up to four orders of magnitude and a sensitivity of 560 mA mmol À 1 L cm À 2. The response time of the electrode to achieve 95% of the steady-state current was recorded at 4 s. The ability of the sensor for routine analyses was demonstrated by the detection of H 2 O 2 presents in milk samples with appreciable recovery values. In addition, the Nafion/EGO/Co 3 O 4-GCE showed good selectivity for H 2 O 2 detection in the presence of ascorbic acid, uric acid, and glucose. The attractive analytical performances such as remarkable catalytic activity, good reproducibility, long term stability, and facile preparation method made this novel nanocomposite electrode promising for the development of effective H 2 O 2 sensor.

European Food Research and Technology, 2008

Hydrogen peroxide (H2O2) residues are not permitted in food due to health concerns. An amperometric sensor based on a Nafion modified palladium (Pd) electrode was developed for the rapid determination of the H2O2 residue in aseptically packaged beverages. A prepared Pd electrode shows a slowly increasing reaction upon repeating the procedure of adding the same concentration of H2O2 into the

International Journal of Environmental Analytical Chemistry, 2020

A non-enzymatic amperometric sensor for hydrogen peroxide (H 2 O 2) was developed based on the screen-printed electrode (SPCE) modified with reduced graphene oxide (RGO), polyneutral red (PNR) and gold nanoparticles (AuNP). Electrochemical behaviour of hydrogen peroxide on SPCE/RGO/PNR/AuNP was investigated. The prepared sensor showed a high electrocatalytic effect on the reduction of hydrogen peroxide. The non-enzymatic sensor response for H 2 O 2 was investigated as a function of pH and working potential. Optimum values of these parameters in the H 2 O 2 determination were found as 7.0 for pH and −0.45 V for working potential. Amperometry was used successfully for the determination of H 2 O 2 in the linear response range from 21.16 to 2730 μM with a detection limit of 6.35 μM. The relative standard deviation (RSD) was calculated to be 1.70% (for n = 10). The operational stability studies have shown that the initial amperometric response of the sensor to H 2 O 2 decreased by 60.82% on the 60th day. Storage life studies have shown that the sensitivity of the sensor decreased by 40.31% at the end of 12 weeks. The non-enzymatic sensor has been tested for H 2 O 2 determination in human serum sample. The developed H 2 O 2 sensor is promising to be used for H 2 O 2 analysis in human serum samples as simple, practical and disposable device without requiring laborious sample pre-treatment producers.

Materials Science and Engineering: C, 2015

In this work, copper nanoparticles (CuNPs) decorated on two new anchored type ligands were utilized to prepare two electrochemical sensors. These ligands are made from bonding amine chains to silica support including SiO 2pro-NH 2 (compound I) and SiO 2-pro-NH-cyanuric-NH 2 (compound II). The morphology of synthesized CuNPs was characterized by transmission electron microscopy (TEM). The nano-particles were in the range of 13-37 nm with the average size of 23 nm. These materials were used to modify carbon paste electrode. Different electrochemical techniques, including cyclic voltammetry, electrochemical impedance spectroscopy and hydrodynamic chronoamperometry, were used to study the sensor behavior. These electrochemical sensors were used as a model for non-enzymatic detection of hydrogen peroxide (H 2 O 2). To evaluate the abilities of the modified electrodes for H 2 O 2 detection, the electrochemical signals were compared in the absence and presence of H 2 O 2. From them, two modified electrodes showed significant responses vs. H 2 O 2 addition. The amperograms illustrated that the sensors were selective for H 2 O 2 sensing with linear ranges of 5.14-1250 μmol L −1 and 1.14-1120 μmol L −1 with detection limits of 0.85 and 0.27 μmol L −1 H 2 O 2 , sensitivities of 3545 and 11,293 μA mmol −1 L and with response times less than 5 s for I/CPE and II/CPE, respectively. As further verification of the selected sensor, H 2 O 2 contained in milk sample was analyzed and the obtained results were comparable with the ones from classical control titration method.

Journal of Food and Drug Analysis, 2016

A new hydrogen peroxide (H 2 O 2) sensor is fabricated based on a multiwalled carbon nanotube-modified glassy carbon electrode (MWCNT-GCE) and reactive blue 19 (RB). The charge transfer coefficient, a, and the charge transfer rate constant, k s , of RB adsorbed on MWCNT-GCE were calculated and found to be 0.44 ± 0.01 Hz and 1.9 ± 0.05 Hz, respectively. The catalysis of the electroreduction of H 2 O 2 by RB-MWCNT-GCE is described. The RB-MWCNT-GCE shows a dramatic increase in the peak current and a decrease in the overvoltage of H 2 O 2 electroreduction in comparison with that seen at an RB modified GCE, MWCNT modified GCE, and activated GCE. The kinetic parameters such as a and the heterogeneous rate constant, k', for the reduction of H 2 O 2 at RB-MWCNT-GCE surface were determined using cyclic voltammetry. The detection limit of 0.27mM and three linear calibration ranges were obtained for H 2 O 2 determination at the RB-MWCNT-GCE surface using an amperometry method. In addition, using the newly developed sensor, H 2 O 2 was determined in real samples with satisfactory results.

Electroanalysis, 2016

The potentiometric response to hydrogen peroxide of a platinum electrode coated with a layer of Nafion is presented. The results show that the Nafion membrane acts as an effective permselective barrier, thus significantly reducing the response to some redox active species, such as ascorbate. Even more interesting, these coated electrodes show a significantly enhanced sensitivity to hydrogen peroxide (H2O2) when the measurements are performed in solutions of high ionic strength. The influence of pH, ionic strength and supporting electrolyte on this enhancement are presented. Under optimized conditions these coated electrodes show a linear dependence with the logarithm of the concentration of H2O2, with sensitivities of −125.1±5.9 mV decade−1 (several times higher than the bare electrodes) and a linear range that spans from 10−5 M to 10−3 M of H2O2. Preliminary studies suggest that the coupling between the redox potential on the Pt electrode and the Donnan potential of the membrane pl...

Current Applied Science and Technology

A rapid, precise, low cost, selective and sensitive paper-based electrochemical device for the determination of H2O2 in milk is described here. Commercially available varnish and a simple hand drawing method were used to develop the hydrophobic pattern to generate a hydrophilic detection zone on the filter paper. The electrode system was fabricated on the detection zone in order to detect H2O2 electrochemically. A commercially available graphite pencil and conductive silver ink were used to fabricate the counter electrode and pseudo-reference electrode, respectively. A paste of Prussian blue (PB) modified graphite, unmodified graphite and phenol-formaldehyde polymer were used to fabricate a PB modified graphite working electrode on paper. This modified electrode showed electrocatalytic activity towards the reduction of H2O2 and it was successfully used for the chronoamperometric detection of H2O2 at 0 V vs Ag reference electrode in 0.1 mol l-1 phosphate buffer, buffered at pH 6.0 in...

Electrochimica Acta, 1998

AbstractÐAn enzymatic biosensor for hydrogen peroxide based on a horseradish peroxidase (HRP)-ferrocene carbon paste modi®ed electrode and coated with a layer of electrochemically generated poly(o-aminophenol) is reported. A linear calibration curve is obtained over the range 1 Â 10 À8 M to 1 Â 10 À5 M. The biosensor responds to hydrogen peroxide in a few seconds and has a detection limit of 8.5 Â 10 À9 M. The response of the biosensor is diusion controlled at low substrate concentrations. Flow injection assays of hydrogen peroxide at a sampling rate of 150 injections per hour with a relative standard deviation of 0.8% (50 samples) are possible. Applicability of the sensor for measurement of hydrogen peroxide in real samples (milk) was demonstrated. #