Stem cell-based approaches in dentistry

Frontiers in Physiology, 2016

Frontiers in physiology, 2014

Stem cells are capable of renewing themselves through cell division and have the remarkable ability to differentiate into many different types of cells. They therefore have the potential to become a central tool in regenerative medicine. During the last decade, advances in tissue engineering and stem cell-based tooth regeneration have provided realistic and attractive means of replacing lost or damaged teeth. Investigation of embryonic and adult (tissue) stem cells as potential cell sources for tooth regeneration has led to many promising results. However, technical and ethical issues have hindered the availability of these cells for clinical application. The recent discovery of induced pluripotent stem (iPS) cells has provided the possibility to revolutionize the field of regenerative medicine (dentistry) by offering the option of autologous transplantation. In this article, we review the current progress in the field of stem cell-based tooth regeneration and discuss the possibilit...

Excli Journal, 2021

Recently, a growing attention has been observed toward potential advantages of stem cell (SC)-based therapies in regenerative treatments. Mesenchymal stem/stromal cells (MSCs) are now considered excellent candidates for tissue replacement therapies and tissue engineering. Autologous MSCs importantly contribute to the state-of-theart clinical strategies for SC-based alveolar bone regeneration. The donor cells and immune cells play a prominent role in determining the clinical success of MSCs therapy. In line with the promising future that stem cell therapy has shown for tissue engineering applications, dental stem cells have also attracted the attention of the relevant researchers in recent years. The current literature review aims to survey the variety and extension of SC-application in tissue-regenerative dentistry. In this regard, the relevant English written literature was searched using keywords: "tissue engineering", "stem cells", "dental stem cells", and "dentistry strategies". According to the available database, SCs application has become increasingly widespread because of its accessibility, plasticity, and high proliferative ability. Among the growing recognized niches and tissues containing higher SCs, dental tissues are evidenced to be rich sources of MSCs. According to the literature, dental SCs are mostly present in the dental pulp, periodontal ligament, and dental follicle tissues. In this regard, the present review has described the recent findings on the potential of dental stem cells to be used in tissue regeneration.

Odontology, 2011

In recent years, stem cell research has grown exponentially due to the recognition that stem cell-based therapies have the potential to improve the life of patients with wide range of conditions, they provides promising methods in vitro as well as in vivo in animal models which make speculation about a future application in human dentistry reasonable. Based on their ability to repair and/or rescue injured tissue and restore organ function even partially, multiple types of stem/progenitor cells have been speculated. Some dental tissues are rich source of mesenchymal stem cells which are suitable for tissue engineering applications. Because they have the potential to differentiate into several cell types, including odontoblasts, neural progenitors, osteoblasts, chondrocytes, and adipocytes. Based on that, an innovativation for generation of clinical material and/or tissue regeneration carried out. Mesenchymal stem cells were founded in dental tissues, including dental pulp, dental papilla, and dental follicle, exfoliated deciduous teeth and periodontal ligament. They can be isolated and grown under defined tissue culture conditions, and used in tissue engineering, including, dental tissue, nerves and bone regeneration.

The Open Dentistry Journal, 2013

Tooth loss or absence is a common condition that can be caused by various pathological circumstances. The replacement of the missing tooth is important for medical and aesthetic reasons. Recently, scientists focus on tooth tissue engineering, as a potential treatment, beyond the existing prosthetic methods. Tooth engineering is a promising new therapeutic approach that seeks to replace the missing tooth with a bioengineered one or to restore the damaged dental tissue. Its main tool is the stem cells that are seeded on the surface of biomaterials (scaffolds), in order to create a biocomplex. Several populations of mesenchymal stem cells are found in the tooth. These different cell types are categorized according to their location in the tooth and they demonstrate slightly different features. It appears that the dental stem cells isolated from the dental pulp and the periodontal ligament are the most powerful cells for tooth engineering. Additional research needs to be performed in or...

Texila International Journal of Academic Research, 2023

Stem cells, also known as progenitor/precursor cells, have the unique trait of self-renewal and multi-lineage differentiation. Dental stem cells (DSCs) are holding a pivotal role during recent times as they thrive as the cornerstone for the development of cell transplantation therapies that correct periodontal disorders and damaged dentin. DSCs are used therapeutically for different organ systems and numerous diseases, including neurological disorders, diabetes, liver disease, bone tissue engineering, and dentistry. In dentistry, the focus is on predominantly regenerating the pulp and damaged dentin, repairing perforations, and periodontal regenerations. Above all, whole tooth regeneration has been constantly under research. The next decade could be a crucial junction where huge leaps in stem cell-based regenerative therapies could become a reality with successful tissue engineering therapies this could be a biological alternative to synthetic materials that are in use currently. But dental stem cells have their share of challenges for which the research must happen effectively adhering to social responsibilities at all levels. Keywords: Stem cells, Regeneration, Regenerative therapy, SHED.

2016

Stem cell (SC) therapy has a promising future for tissue regenerative medicine. However, because SC technology is still in its infancy, interdisciplinary cooperation is needed to achieve successful clinical applications. Dental SCs have drawn attention in recent years because of their accessibility, plasticity, and high proliferative ability. Based on their ability to rescue and/or repair injured tissue and partially restore organ function, multiple types of stem/progenitor cells have been speculated. Growing evidence demonstrates that stem cells are primarily found in niches and that certain tissues contain more stem cells than others. Among these tissues, the dental tissues are considered a rich source of mesenchymal stem cells that are suitable for tissue engineering applications.Several types of dental SCs have been identified, including dental pulp SCs from adult human dental pulp, SCs from human primary exfoliated deciduous teeth, periodontal ligament SCs, and dental follicle ...

Stem Cell Reviews and Reports, 2011

The accelerated pace of research in the stem cell field in recent decades and the accumulated body of knowledge has spurred the interest in potential clinical applications of stem cells in all branches of medicine including regenerative dentistry. In humans, embryonic and adult stem cells are two major groups of cells that can serve as a donor source in tissue engineering strategies based on ex-vivo cellular expansion. It has been shown that adult stem cell populations are present in all examined living tissues of the organism, thus being a crucial source of tissue homeostasis and regeneration, and offering a target population for in situ stimulation of extensive tissue regeneration. Experimental findings indicate that in the complex structure of the tooth organ, both periodontal and endodontic tissues harbour adult stem cells with characteristics peculiar to early stages of cellular differentiation. Myriad of strategies incorporating both embryonic and adult stem cells for the regeneration of a particular tooth structure or the whole teeth were proposed; however their successful application to solve real problems encountered in the clinical practice of dentistry remains an elusive and challenging objective.

The Journal of Contemporary Dental Practice, 2009

Aim The aim of this paper is to present a review and discussion of the current status of stem cell research with regard to tooth generation. Background Stem cells have been isolated from the pulp tissue of both deciduous and permanent teeth as well as from the periodontal ligament. Dental pulp stem cells demonstrate the capacity to form a dentin pulp-like complex in immunocompromised mice. A tooth-like structure was successfully formed, using a heterogeneous mixture of dental enamel epithelium, pulp mesenchymal cells, and scaffolds. Conclusion The scientific community understands the need for more investigations to completely understand the conditions that would best favor the creation of a tooth substitute. Recent gains in the understanding of the molecular regulation of tooth morphogenesis, stem cell biology, and biotechnology offers the opportunity to realize this goal. Clinical Significance These findings, combined with the recent progress in stem cell research and tissue engine...

2018

DOI: 10.21276/sjbr.2018.3.3.2 Abstract: Periodontitis are multifactorial infections that begin with a period of inflammation of the supporting tissues of the teeth and then progress destroying the tissues until loss of the teeth. The goal of periodontal treatment is to stop the progression of the disease, restore functions and regenerate the damaged tissue. However, regeneration of different tissues, by conventional treatments, still remains great challenge. To address this, there are several approaches to tissue engineering for regenerative dentistry, among them, the use of stem cells by including dental pulp stem cells (DPSCS), periodontal ligament stem cells (PDLSCS), stem cells from the dental apical papilla (SCAPS), stem cells from human exfoliated deciduous teeth (SHEDS), dental follicle stem cells (DFSCS), dental epithelial stem cells (DESCS), bone marrow mesenchymal stem cells (BMMSCS), adipose-derived stem cells (ADSCS), embryonicstem cells (ESCS) and induced pluripotent st...