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2018, Seibutsu Butsuri
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3 pages
1 file
Seibutsu Butsuri, 2007
Progress of Theoretical Physics, 2009
2016 18th International Conference on Transparent Optical Networks (ICTON), 2016
Hyōmen kagaku, 2001
Proteins and polypeptides were first covalently immobilized on a solid surface and then extended by a tensile force applied at the two ends of the polymer chain. The method consisted of introducing cysteine residues at N-and C-termini of protein molecules and covalently immobiling them on an amino-silanized surface of a crystalline silicon wafer. The other end of the protein molecule was cross-linked to a functionalized AFM (atomic force microscope) tip with covalent cross-linkers. The relationship between the tensile force and the extension length of the molecule was measured using the force curve mode of AFM. Results obtained with a polyglutamic acid that takes helical conformation in acidic and random coil state in neutral and alkaline media is described. Also the relationships measured with β-sheet globular protein, carbonic dehydratase are given.
The Japan Society of Applied Physics, 2017
Hyomen Kagaku, 1998
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Seibutsu Butsuri, 2003
Seibutsu Butsuri, 2001
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