Dario Piombino-Mascali
Antropologo specializzato in mummiologia e paleopatologia all'Università di Vilnius, esploratore della National Geographic Society e docente a contratto di paleoantropologia e antropologia forense all'Università degli Studi di Messina, mi occupo prevalentemente dello studio scientifico di mummie di età medievale e moderna, di reliquie e di corpi santi, così come di museologia scientifica applicata alle collezioni anatomiche e patologiche. Collaboro da tempo con i Musei Vaticani, il Museo "Paolo Gorini", il Museo Nazionale delle Filippine e il Museo Archeologico dell'Alto Adige. Ho organizzato seminari sullo studio dei resti umani in vari atenei italiani e stranieri, tra cui quelli di Pisa, Bari, Oxford, Kaunas, Catania e Cagliari, e ho insegnato per le Università di Edimburgo, Cranfield e Tartu. Ho cooperato, come divulgatore, con numerosissime testate giornalistiche e televisive nazionali ed internazionali. Sono anche conservatore scientifico delle celebri Catacombe dei Cappuccini di Palermo e guida turistica nazionale.
Supervisors: Gino Fornaciari, Francesco Mallegni, and Roger Wilson
Phone: +37052398709
Address: c/o Anatomicum, Faculty of Medicine, Vilnius University, MK Čiurlionis street 21, LT-03101, Vilnius, Lithuania
Supervisors: Gino Fornaciari, Francesco Mallegni, and Roger Wilson
Phone: +37052398709
Address: c/o Anatomicum, Faculty of Medicine, Vilnius University, MK Čiurlionis street 21, LT-03101, Vilnius, Lithuania
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Peer-Reviewed Journal Articles by Dario Piombino-Mascali
In this study, we introduce an innovative new method using linear polyacrylamide (LPA) to efficiently precipitate and purify nucleic acids extracted from complex environmental and ancient tissue samples in one working step. The LPA method replaces the precipitation step in classic liquid-phase/organic extraction protocols or can be easily applied as an additional post-extraction step on impure DNA extracts. As a proof of concept, we experimented with this method on different ancient human mummy samples (bones, soft tissues and gut contents) from different time periods (5000 BC–1800 AD), as well as on complex environmental samples (e.g. soil, activated sludge and animal faeces) known to contain inhibitory compounds.
We demonstrated that LPA precipitates nucleic acids, even in an aqueous ethanol solution without the addition of chaotropic salts, resulting in the recovery of highly pure DNA from all tested samples that displayed inhibition with previously published extraction protocols. Compared to the current, most widely used silica-based extraction method for ancient and sedimentary DNA, our LPA method resulted in comparable DNA qualities and overall DNA compositions (human endogenous content and microbial diversity).
In conclusion, our LPA method with its high purifying capacity provides an important alternative to the commonly used DNA extraction protocols in the environmental and ancient DNA (aDNA) fields.
In this study, we introduce an innovative new method using linear polyacrylamide (LPA) to efficiently precipitate and purify nucleic acids extracted from complex environmental and ancient tissue samples in one working step. The LPA method replaces the precipitation step in classic liquid-phase/organic extraction protocols or can be easily applied as an additional post-extraction step on impure DNA extracts. As a proof of concept, we experimented with this method on different ancient human mummy samples (bones, soft tissues and gut contents) from different time periods (5000 BC–1800 AD), as well as on complex environmental samples (e.g. soil, activated sludge and animal faeces) known to contain inhibitory compounds.
We demonstrated that LPA precipitates nucleic acids, even in an aqueous ethanol solution without the addition of chaotropic salts, resulting in the recovery of highly pure DNA from all tested samples that displayed inhibition with previously published extraction protocols. Compared to the current, most widely used silica-based extraction method for ancient and sedimentary DNA, our LPA method resulted in comparable DNA qualities and overall DNA compositions (human endogenous content and microbial diversity).
In conclusion, our LPA method with its high purifying capacity provides an important alternative to the commonly used DNA extraction protocols in the environmental and ancient DNA (aDNA) fields.
"Ancient Ink," the first book dedicated to the archaeological study of tattooing, presents new research from across the globe examining tattooed human remains, tattoo tools, and ancient art. It contributes to our understanding of the antiquity, durability, and significance of tattooing and human body decoration and illuminates how different societies have used their skin to construct their identities. Ancient Ink connects ancient body art traditions to modern culture through Indigenous communities and the work of contemporary tattoo artists.
The contributors are Orlando V. Abinion, Analyn Salvador-Amores, Gemma Angel, Ronald G. Beckett, Tara Nicole Clark, Colin Dale, Aaron Deter-Wolf, Renée Friedman, Louise Furey, Lars Krutak, Svetlana Pankova, Dario Piombino-Mascali, Luc Renaut, Benoît Robitaille, Dong Hoon Shin, Isaac Walters, Leonid Yablonsky, and Petar N. Zidarov.
Progetto di studio dei resti mortali.