Papers by David Lázaro-Gimeno
<p>Genes shown inside the circle are transcribed clockwise and genes outside are transcribe... more <p>Genes shown inside the circle are transcribed clockwise and genes outside are transcribed counter clockwise. Genes for tRNAs are represented by one letter code amino acids with anticodons. Asterisks indicate genes with introns. Pseudogenes are preceded by the Ψ symbol.</p
Plants
Among the most intriguing mysteries in the evolutionary biology of photosynthetic organisms are t... more Among the most intriguing mysteries in the evolutionary biology of photosynthetic organisms are the genesis and consequences of the dramatic increase in the mitochondrial and nuclear genome sizes, together with the concomitant evolution of the three genetic compartments, particularly during the transition from water to land. To clarify the evolutionary trends in the mitochondrial genome of Archaeplastida, we analyzed the sequences from 37 complete genomes. Therefore, we utilized mitochondrial, plastidial and nuclear ribosomal DNA molecular markers on 100 species of Streptophyta for each subunit. Hierarchical models of sequence evolution were fitted to test the heterogeneity in the base composition. The best resulting phylogenies were used for reconstructing the ancestral Guanine-Cytosine (GC) content and equilibrium GC frequency (GC*) using non-homogeneous and non-stationary models fitted with a maximum likelihood approach. The mitochondrial genome length was strongly related to rep...
Plant Cell, Tissue and Organ Culture (PCTOC), 2014
ABSTRACT The potential of haploid tissues for genetic studies in conifers is hampered by the lack... more ABSTRACT The potential of haploid tissues for genetic studies in conifers is hampered by the lack of abundant and homogeneous plant material suitable for DNA isolation. In this work we have determined factors promoting haploid callus induction and proliferation from megagametophytes of Oria 6, a genotype of Pinus pinaster Aiton (maritime pine) from the natural population Sierra de Oria (Almería, Spain), selected based on its response to extreme drought conditions. The generation of haploid callus was restricted to megagametophytes isolated from light-brown cones with no dehydrated seeds collected in September. Culture medium composition did not significantly affect callus induction, but a modified Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid and 6-benzyladenine favored further multiplication. The ploidy status of the callus lines was determined by flow cytometry and seven polymorphic microsatellites. A total of sixteen haploid callus lines were established and one of these is being used as a source of DNA for massive sequencing of maritime pine genome.
Los fotooxidantes, y especialmente el ozono, han sido considerados como dañinos para la vegetació... more Los fotooxidantes, y especialmente el ozono, han sido considerados como dañinos para la vegetación desde 1980 en Europa. Se habla del ozono como un contaminante secundario, debido a su origen, y sin embargo sus efectos sobre las masas forestales pueden ser considerados como de primer orden. Por ello continuamente se van mejorando los métodos Recibido el 26 de mayo de 2008, aceptado para su publicación el 11 de noviembre de 2008
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Papers by David Lázaro-Gimeno