Conference Presentations by Gladys Ilagan
Methicillin-resistant Staphylococcus aureus (MRSA) is the most prevalent nosocomial infection in ... more Methicillin-resistant Staphylococcus aureus (MRSA) is the most prevalent nosocomial infection in both developing and developed countries. Methicillin resistance is due to the presence of mecA gene which is highly conserved among MRSA strains. The study aims to develop loop-mediated isothermal amplification (LAMP) as a detection method in hospital-associated samples of MRSA (HA-MRSA). A set of four primers were designed based on the mecA gene of MRSA. LAMP parameters such as MgCl2 concentration, denaturation time, incubation time, incubation temperature and primer concentration were optimized. DNA of MRSA and methicillin sensitive Staphylococcus aureus (MSSA) were used as templates for amplification. LAMP products were visualized using agarose gel. Using the optimized LAMP conditions, the assay was used to detect MRSA from clinical samples obtained and authenticated from the Department of Pathology Laboratory in the Armed Forces of the Philippines Medical Center. The LAMP assay was able to detect the mecA gene of MRSA using the designed primers and the optimized conditions. Also, the assay showed a high degree of specificity to MRSA against the sensitive strains. LAMP method is a simple, fast, inexpensive and sensitive tool that can be used to rapidly detect MRSA for routine clinical diagnostics in resource-limited settings.
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Conference Presentations by Gladys Ilagan