Ingenieria

The laboratory rat (Rattus norvegicus) is an indispensable tool in experimental medicine and drug development, having made inestimable contributions to human health. We report here the genome sequence of the Brown Norway (BN) rat strain. The sequence represents a high-quality 'draft' covering over 90% of the genome. The BN rat sequence is the third complete mammalian genome to be deciphered, and three-way comparisons with the human and mouse genomes resolve details of mammalian evolution. This first comprehensive analysis includes genes and proteins and their relation to human disease, repeated sequences, comparative genome-wide studies of mammalian orthologous chromosomal regions and rearrangement breakpoints, reconstruction of ancestral karyotypes and the events leading to existing species, rates of variation, and lineage-specific and lineageindependent evolutionary events such as expansion of gene families, orthology relations and protein evolution.

Biósfera La biosfera es la delgada capa de la tierra y su atmósfera que cubre la superficie del planeta, y en la que viven todos los seres vivos. Es una zona relativamente delgada que está formada por los océanos, lagos y ríos, la tierra firme y la parte inferior de la atmósfera, que es capaz de mantener la vida en el planeta.

In the course of a study conducted to isolate genes upregulated by plant cell wall sugars, we identified an arabinose-inducible locus from a transcriptional fusion library of Rhizobium leguminosarum VF39, carrying random insertions of the lacZ transposon Tn5B22. Sequence analysis of the locus disrupted by the transposon revealed a high similarity to uncharacterized malate synthase G genes from Sinorhizobium meliloti, Agrobacterium tumefaciens, and Mesorhizobium loti. This enzyme catalyzes the condensation of glyoxylate and acetyl-CoA to yield malate and CoA and is thought to be a component of the glyoxylate cycle, which allows microorganisms to grow on two carbon compounds. Enzyme assays showed that a functional malate synthase is encoded in the glcB gene of R. leguminosarum and that its expression is induced by arabinose, glycolate, and glyoxylate. An Escherichia coli aceB glcB mutant, complemented with the R. leguminosarum PCR-amplified gene, recovered malate synthase activity. A very similar genome organization of the loci containing malate synthase and flanking genes was observed in R. leguminosarum, S. meliloti, and A. tumefaciens. Pea plants inoculated with the glcB mutant or the wild-type strain showed no significant differences in nitrogen fixation. This is the first report regarding the characterization of a mutant in one of the glyoxylate cycle enzymes in the rhizobia.

During 15 August through 30 September 2006, ground and aircraft measurements were obtained from a multi-national group of students and scientists in Senegal. Key measurements were aimed at investigating and understanding precipitation processes, thermodynamic and dynamic environmental conditions, cloud, aerosol and microphysical processes and spaceborne sensors (TRMM, CloudSat/Calipso) validation. Ground and aircraft instruments include: ground based polarimetric radar, disdrometer measurements, a course and a high-density rain gauge network, surface chemical measurements, a 10 m flux tower, broadband IR, solar and microwave measurements, rawinsonde and radiosonde measurements, FA-20 dropsonde, in situ microphysics and cloud radar measurements. Highlights during SOP3 include ground and aircraft measurements of squall lines, African Easterly Waves (AEWs), Saharan Air Layer advances into Senegal, and aircraft measurements of AEWs-including the perturbation that became Hurricane Isaac.

Eclipse 1. ¿Qué decisión tomaría usted como gerente de mercadotecnia de Eclipse, para darla a conocer al gerente general, de asignar o no marca a sus productos, de forma que garantice una alta probabilidad de éxito? Dar la propuesta de nuevas alternativas para darle un nombre a la marca e iniciar a trabajar en promociones para darla a conocer, aunque las ventas sean elevadas, se necesita abrir y/o expandir el mercado en el interior y en el exterior. Lanzar promociones y publicidad por medios informáticos así como redes sociales entre páginas de interés del consumidor así como por medios de telecomunicaciones En el supuesto de que fuera beneficio no poner ahora marca a los productos, ¿recomendaría que a largo plazo se hiciera? Si, es necesario dar a conocer la marca en el exterior y si es posible tener una visión para que no sea reconocida en el país también sea en el extranjero.

A life-cycle energy consumption analysis of a Bridgeport manual mill and a Mori Seiki DuraVertical 5060 has been conducted. The use phase incorporated three manufacturing environments: a community shop, a job shop, and a commercial facility. The CO2-equivalent emissions were presented per machined part. While the use phase comprised the majority of the overall emissions, the manufacturing phase emissions were significant especially for the job shop, which is not as efficient as the other facilities due to its inherent need for flexibility. Since the Mori Seiki is heavier, the manufacturing phase of this machine tool had a greater impact on emissions than the Bridgeport. Transportation was small relative to the use phase, which was dominated by cutting, HVAC, and lighting. These results highlight areas for energy reductions in machine tool design as well as the importance of facility type to the manufacture of any product.

Since machine tools are used extensively throughout their functional life and consequently consuming valuable natural resources and emitting harmful pollutants during this time, this study reviews strategies for characterizing and reducing the energy consumption of milling machine tools during their use. The power demanded by a micromachining center while cutting low carbon steel under varied material removal rates was measured to model the specific energy of the machine tool. Thereafter the power demanded was studied for cutting aluminum and polycarbonate work pieces for the purpose of comparing the difference in cutting power demand relative to that of steel.

Background: Drugs can induce different immunologic reactions; T-cell mediated responses produce the most severe reactions. Although in vitro studies show that T cells recognize drugs or their metabolites and induce an effector cytotoxic response, direct in vivo evidence of involvement is lacking. T lymphocytes produce cytotoxic markers that are responsible for 2 major pathways to cell death: granule-mediated exocytosis (perforin and granzyme B) and Fas/FasL interaction. Objective: The purpose of this investigation was to establish the role of proinflammatory TNF-α and cytotoxic markers in subjects with delayed responses to drugs.

Background: In nonimmediate cutaneous reactions to drugs, the skin is the organ most frequently involved, and T cells may play a relevant role. T cells related to skin immune responses express the cutaneous lymphocyte-associated antigen (CLA), the skin-homing receptor.Methods: We studied the expression of the CLA in peripheral blood T cells from nine subjects with exanthematous reactions induced by β-lactams ( 4), phenytoin ( 2), propyphenazone ( 1), spiramycin plus metronidazol ( 1), and captopril plus tiazide ( 1). The cutaneous symptoms appeared at least 6 h after drug intake. CLA expression was evaluated by flow cytometry at the time of the reaction (T1) and 1 month later (T2). HLA-DR activation marker expression was also evaluated at T1. In four patients, it was necessary to readminister the culprit drug to establish a causal relationship, and sequential estimation of the markers was performed. Two control groups were included: healthy controls and subjects exposed to the culprit drugs with good tolerance. Values were compared by nonparametric statistics.Results: The expression of circulating CLA+ T cells at T1 was increased compared to healthy controls (median=20.4 vs 9.4) (P<0.001), and the patients also expressed increased levels of HLA-DR (median=3.8) (P<0.005). Comparison between T1 and T2 (median=11.2) also showed differences in levels of CLA+ T cells (P<0.01). The patients re-exposed to the culprit drug showed an increase followed by a decrease of circulating CLA+ T cells (P<0.05) and CLA+ HLA-DR+(P<0.05) paralleling the symptoms.Conclusions: These data support the immunologic nature of delayed skin reactions to drugs, and suggest that these CLA+ T cells parallel the disease evolution and may participate in the pathophysiologic mechanisms.

Background: Penicillin is no longer the most commonly prescribed b-lactam, and the pattern of reactions has changed. We studied the diagnostic value of skin testing in penicillin-allergic subjects from a population where benzylpenicillin is not now the most frequently used b-lactam. Methods: Patients with a history of immediate allergic reactions to penicillins were studied with: skin tests with major and minor determinants of benzylpenicillin (BPO/MDM), amoxicillin, and ampicillin; in vitro determination of specific IgE; and controlled administration for those with a positive history but negative skin and in vitro tests. A reaction was considered immediate when symptoms appeared within a maximum of 1 h after drug intake. Results: After testing, 290 patients (71% having anaphylaxis and 29% having urticaria) proved to be allergic. Amoxicillin was involved in 64.8% and benzylpenicillin in 2.8% of the patients. Skin test positivity to at least one determinant appeared in 70% of cases, amoxicillin being the most frequent. The overall sensitivity decreased markedly when only BPO and MDM were considered. In 13.1% of patients, the diagnosis was established by in vitro test and in 16.9% by controlled administration. Of the 290 patients, 42.1% were positive to determinants generated from benzylpenicillin and 57.9% were selective responders. Conclusions: Sensitivity of skin tests to BPO was lower than reported, being partly replaced by minor determinants, mostly amoxicillin. The incorporation of additional reagents and the development of new tests are required, and these will probably change as the patterns of consumption vary.