Despite the proliferation of proteins that can form filaments or phase-separated condensates, it ... more Despite the proliferation of proteins that can form filaments or phase-separated condensates, it remains unclear how this behavior is distributed over biological networks. We have found that 60 of the 440 yeast metabolic enzymes robustly form structures, including 10 that assemble within mitochondria. Additionally, the ability to assemble is enriched at branch points on several metabolic pathways. The assembly of enzymes at the first branch point in de novo purine biosynthesis is coordinated, hierarchical, and based on their position within the pathway, while the enzymes at the second branch point are recruited to RNA stress granules. Consistent with distinct classes of structures being deployed at different control points in a pathway, we find that the first enzyme in the pathway, PRPP synthetase, forms evolutionarily conserved filaments that are sequestered in the nucleus in higher eukaryotes. These findings provide a roadmap for identifying additional conserved features of metabo...
Recent work has found that many metabolic enzymes have the ability to polymerize in response to m... more Recent work has found that many metabolic enzymes have the ability to polymerize in response to metabolic changes or environmental stress. This ability to polymerize is well conserved for the few metabolic enzyme paralogs that have been studied in yeast. Here we describe the first set of paralogs, Asn1p and Asn2p, that have differential assembly behavior. Asn1p and Asn2p both co-assemble into filaments in response to nutrient limitation. However, the ability of Asn2p to form filaments is strictly dependent on the presence of Asn1p. Using mutations that block enzyme activity but have differential effects on Asn1p polymerization, we have found that Asn1p polymers are unlikely to have acquired a moonlighting function. Together these results provide a novel system for understanding the regulation and evolution of metabolic enzyme polymerization.
Moderate El Siyasi: Jurnal Pemikiran Politik Islam
Tulisan akan membahas pandangan Islam tentang Kementerian (al-wizarah), dimulai dari definisi, da... more Tulisan akan membahas pandangan Islam tentang Kementerian (al-wizarah), dimulai dari definisi, dalil-dalil pensyari’atannya, sejarah dan perkembangannya. Terkait sejarah dan perkembangannya, penulis hanya akan menyoroti dari masa Nabi Muhammad SAW hingga masa Dinasti Abbasiyah. Dari tulisan ini disimpulkan bahwa Alquran dan sejarah kenabian telah menunjukkan konsep seminal tentang kementerian (al-Wizarah). Konsepsi kementerian (al-wizarah) dalam Islam, sejatinya merujuk pada makna linguistik, dan pandangan Alquran (quranic worldview), yaitu sebagai penolong, atau pembantu pemimpin (imam atau khalifah) dalam mengurus kepemerintahan. Dalam catatan sejarah Islam (khusus dari zaman Nabi hingga Abbasiyah), terlihat kementerian telah mengalami pengembangan baik secara kelembagaan, fungsi dan perannya. Dilihat dari fungsi dan perannya, al-Wizarah dalam sejarah peradaban Islam memiliki dua bentuk yaitu Wizarah at-tafwid (Kementerian Eksekutif) dan Wizarah at-tanfidhi (Kementerian Mandataris...
Despite the proliferation of proteins that can form filaments or phase-separated condensates, it ... more Despite the proliferation of proteins that can form filaments or phase-separated condensates, it remains unclear how this behavior is distributed over biological networks. We have found that 60 of the 440 yeast metabolic enzymes robustly form structures, including 10 that assemble within mitochondria. Additionally, the ability to assemble is enriched at branch points on several metabolic pathways. The assembly of enzymes at the first branch point in de novo purine biosynthesis is coordinated, hierarchical, and based on their position within the pathway, while the enzymes at the second branch point are recruited to RNA stress granules. Consistent with distinct classes of structures being deployed at different control points in a pathway, we find that the first enzyme in the pathway, PRPP synthetase, forms evolutionarily conserved filaments that are sequestered in the nucleus in higher eukaryotes. These findings provide a roadmap for identifying additional conserved features of metabo...
Recent work has found that many metabolic enzymes have the ability to polymerize in response to m... more Recent work has found that many metabolic enzymes have the ability to polymerize in response to metabolic changes or environmental stress. This ability to polymerize is well conserved for the few metabolic enzyme paralogs that have been studied in yeast. Here we describe the first set of paralogs, Asn1p and Asn2p, that have differential assembly behavior. Asn1p and Asn2p both co-assemble into filaments in response to nutrient limitation. However, the ability of Asn2p to form filaments is strictly dependent on the presence of Asn1p. Using mutations that block enzyme activity but have differential effects on Asn1p polymerization, we have found that Asn1p polymers are unlikely to have acquired a moonlighting function. Together these results provide a novel system for understanding the regulation and evolution of metabolic enzyme polymerization.
Moderate El Siyasi: Jurnal Pemikiran Politik Islam
Tulisan akan membahas pandangan Islam tentang Kementerian (al-wizarah), dimulai dari definisi, da... more Tulisan akan membahas pandangan Islam tentang Kementerian (al-wizarah), dimulai dari definisi, dalil-dalil pensyari’atannya, sejarah dan perkembangannya. Terkait sejarah dan perkembangannya, penulis hanya akan menyoroti dari masa Nabi Muhammad SAW hingga masa Dinasti Abbasiyah. Dari tulisan ini disimpulkan bahwa Alquran dan sejarah kenabian telah menunjukkan konsep seminal tentang kementerian (al-Wizarah). Konsepsi kementerian (al-wizarah) dalam Islam, sejatinya merujuk pada makna linguistik, dan pandangan Alquran (quranic worldview), yaitu sebagai penolong, atau pembantu pemimpin (imam atau khalifah) dalam mengurus kepemerintahan. Dalam catatan sejarah Islam (khusus dari zaman Nabi hingga Abbasiyah), terlihat kementerian telah mengalami pengembangan baik secara kelembagaan, fungsi dan perannya. Dilihat dari fungsi dan perannya, al-Wizarah dalam sejarah peradaban Islam memiliki dua bentuk yaitu Wizarah at-tafwid (Kementerian Eksekutif) dan Wizarah at-tanfidhi (Kementerian Mandataris...
Uploads
Drafts by AHMAD ADHA
Papers by AHMAD ADHA