Papers by Alwan Al Qushawi
One sampling station was selected to conduct the study in Al-Gharraf River in Kut-Al-Haay city. T... more One sampling station was selected to conduct the study in Al-Gharraf River in Kut-Al-Haay city. The samples were taken from the study station at once every month from June of 2017 until May 2018 for year. The study results showed that the mean concentrations heavy metals are vary seasonally in their levels and analyses of muscles indicate that Ni occupied the first position and revealed the highest levels, in general. The minimal recorded accumulative value (0.187ppm) was in spring and the highest (0.251 ppm) in summer. Zinc occupied second accumulation levels and showed the minimal (0.039 ppm) in spring, whereas Cd, by no means, exhibited the minimal concentrations and the lowest value (0.0053 ppm) was in winter.
Article in Iranian journal of pharmaceutical research (IJPR) · December 2016 CITATION 1 7 authors... more Article in Iranian journal of pharmaceutical research (IJPR) · December 2016 CITATION 1 7 authors, including: Some of the authors of this publication are also working on these related projects:
Tilmicosin (TLM) is an important antibiotic in veterinary medicine with low bioavailability and s... more Tilmicosin (TLM) is an important antibiotic in veterinary medicine with low bioavailability and safety. This study aimed to formulate and evaluate physicochemical properties, storage stability after lyophilization, and antibacterial activity of three TLM-loaded lipid nanoparticles (TLM-LNPs) including solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs), and lipid-core nanocapsules (LNCs). Physicochemical parameters such as particle size-mean diameter, polydispersity index, zeta potential, drug encapsulation efficiency (EE), loading capacity, and morphology of the formulations were evaluated and the effects of various cryoprotectants during lyophilization and storage for 8 weeks were also studied. The profiles of TLM release and the antibacterial activities of these TLM-LNPs suspensions (against Escherichia coli and Staphylococcus aureus) were tested in comparison with their corresponding powders. TLM-LNPs suspensions were in nano-scale range with mean diameters of 186.3 ± 1.5, 149.6 ± 3.0, and 85.0 ± 1.0nm, and also EE, 69.1, 86.3, and 94.3% for TLM-SLNs, TLM-NLCs, and TLM-LNCs respectively. TLM-LNCs gave the best results with significantly low particle size and high EE (p<0.05). Mannitol was the most effective cryoprotectant for lyophilization and storage of TLM-LNPs. The drug release profiles were biphasic and the release times were longer at pH 7.4 where TLM-NLCs and TLM-LNCs powders showed longer release times. In microbiological tests, S. aureus was about 4 times more sensitive than E. coli to TLM-LNPs with minimum inhibitory concentration ranges of 0.5-1.0 and 2-4 µg/mL respectively, and TLM-LNCs exhibited the best antibacterial activities. In conclusion, TLM-LNP formulations especially TLM-LNCs and TLM-NLCs are promising carriers for TLM with better drug encapsulation capacity, release behavior, and antibacterial activity.
The study was carried out to investigate some toxicological effects and invitro antibacterial act... more The study was carried out to investigate some toxicological effects and invitro antibacterial activity of Datura innoxia dried leaves.The yield percentage of Datura innoxia 95% ethyl alcohol leaves extract was 19%. Phytochemicals analysis of both Datura innoxia leaves powder and alcoholic extract, indicated the presence of alkaloids, phenols, glycosides, tannins, resins, saponins, flavonoids and steroids which are responsible for toxic and therapeutic effects. The acute toxicological effect of Datura innoxia ethanolic extract in male rats was determined by estimation the oral median lethal dose (LD 50 ) by up and down method was 1214 mg/kg. BW. The in-vitro antibacterial activity of Datura innoxia ethanolic extract and its minimum inhibitory concentration activity (MIC) showed that E. coli was more sensitive to D.I ethanolic extract than Staph. aureus, the MIC for D.I extract against E. coli was less than MIC against Staph. aureus which was 5 and 10 mg/ml respectively, also E. coli was more sensitive to ciprofloxacin .
Tilmicosin (TLM) is a semisynthetic antimicrobial agent used mainly in poultry and cattle, but it... more Tilmicosin (TLM) is a semisynthetic antimicrobial agent used mainly in poultry and cattle, but it has relatively poor oral bioavailability. This study was conducted to compare the bioavailability (BA) and main pharmacokinetic (PK) parameters of TLM after oral administration of tilmicosin phosphate (TLM-PH) and three newly prepared lipid nanoparticles (LNPs) of TLM including solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs), and lipid-core nanocapsules (LNCs). Sixty broiler chickens were divided into eight groups. In four treatment groups (n = 10), each bird was given a single oral dose (20 mg/kg) of a TLM formulation after overnight fasting, and in four control groups (n = 5), the vehicles of those formulations or distilled water were given. Plasma TLM concentrations were analyzed using an HPLC method and the related PK parameters (C max , T max , AUC 0-∞ , t 1/2 , k el , Cl B /F, MRT, and V d /F) were obtained by noncompartmental analysis. The relative bioavailability of TLM-SLNs, TLM-NLCs, and TLM-LNCs were 1.7, 2.7, and 3.6 times, respectively, more than the BA of TLM-PH. Mean C max values were 1.21, 1.58, 1.76, and 2.17 µg/mL for TLM-PH, TLM-SLN, TLM-NLC, and TLM-LNC, respectively. In conclusion, TLM-LNPs improved drug BA and PK parameters, especially the TLM-LNC formulation, which suggests an efficient delivery system for TLM.
Keywords Abbreviations www.IJVST.um.ac.ir Despite the development of novel drugs and technologies... more Keywords Abbreviations www.IJVST.um.ac.ir Despite the development of novel drugs and technologies in combating the infectious diseases, they remain as a global health challenge. The use of conventional antimicrobial drugs are always associated with problems such as antimicrobial resistance, adverse effects, and inefficient drug delivery. In this regard, the unique physiochemical properties of the nanoparticles have led to increase in the researches on nanoparticles and their application as promising antimicrobial products. Lipid nanoparticles (LNPs) are new carrier systems developed as an alternative to traditional nanoparticle vehicles. The solid lipid nanoparticles (SLN), nanostructured lipid carriers (NLCs), lipid-drug conjugates (LDCs), Lipid-core nanocapsules (LNCs) and lipid-polymer nanoparticles (LPNs) are carriers with a lipid matrix showing advantages for different applications due to the use of biodegradable, and biocompatible lipids. LNPs have exclusive properties owing to their ultra-small size and high surface area, thereby, they are able to increase drug targeting, treatment efficiency and reduce the toxicity of antimicrobial drugs. LNPs are often used as sustained release systems, and they also used for improving drug bio-availability. It has been found that the composition and structure of LNPs are two critical factors that may influence their pharmaceutical performance in different body organs. This review focuses on the development of LNP systems for antimicrobial drugs delivery and gives an overview on the modern LNP-based therapeutic strategies against the infections. The mechanism of action and advantages of these nanoparticles as antibacterial, antifungal, antiviral and anti-parasitic agents are highlighted in this review.
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Papers by Alwan Al Qushawi