The aim of this study was of twofold. One was to determine the genetic structure of Iraq populati... more The aim of this study was of twofold. One was to determine the genetic structure of Iraq population and the second objective of the study was to evaluate the importance of these loci for forensic genetic purposes. FTA® Technology (FTA™ paper DNA extraction) utilized to extract DNA. Twenty (20) STR loci and Amelogenin), including D3S1358, D13S317, and Amelogenin amplified by using power plex21® kit. Polymerase chain reaction (PCR) products detected by genetic analyzer 3730xL then data analyzed by PowerStatsV1.2. Based on the allelic frequencies, several statistical parameters of genetic and forensic efficiency have been estimated. This includes the homozygosity and heterozygosity, effective number of alleles (n), the polymorphism information content (PIC), the power of discrimination (DP) and the power of exclusion (PE). The power of discrimination values for all tested loci was from 75 to 96%; therefore, those loci can be safely used to establish a DNA-based database for Iraq population. The high PIC values of the selected markers confirm their usefulness for genetic polymorphism studies and linkage mapping programs in human as well. The mean heterozygosity observed, is expected to have mean PIC values across the 20 loci which were 0.77, 0.81 and 0.78, respectively, indicating high gene diversity.
The main objective of this study was to determine the phytochemical composition in the leaves of
... more The main objective of this study was to determine the phytochemical composition in the leaves of Coriandrum sativum, using methanolic extraction and report the main functional components by using IR technique. The phytochemical compounds in the extract were then screened by GC-MS method. Seven bioactive phytochemical compounds were identified in the methanolic extract of C. sativum: 1,6- octadien-3-ol, 3,7-dimethyl, 1,6-octadien-3-ol,3,7-dimethyl, 2-aminobenzoate, bicyclo[2.2.1]heptan-2- one,1,7,7-trimethyl., geranyl vinyl ether, 9,10-secocholesta-5,7,10(19)-triene-3,24,25-triol., ascorbic acid 2,6-dihexadecanoate and 7aH-cyclopenta[a] cyclopropa[f]cycloundecene. Thirteen bioactive phytochemical compounds were identified in the methanolic extract of Melia azedarach. In the present investigation, a variety of compounds have been detected in M. azedarach including trichloromethane, propanedioic acid, diethyl ester, 2-pyrrolidinyl-methylamine, butanedioic acid, diethyl ester, 2- piperidimethanamine, butanedioic acid, hydroxyl-, diethyl ester, 2,5-dimethylhexane-2,5- dihydroperoxide, dithiocarbamate, s-methyl-,n-(2-methyl-3-oxobutyl), triethyl citrate, y-sitosterol, ethyl 9,12,15-octadecatrienoate, hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, and octadecane, 3-ethyl-5-(2-ethylbutyl). It contains chemical constitutions which may be useful for various herbal formulation as anti-inflammatory, analgesic, antipyretic, cardiac tonic and antiasthamatic. C. sativum is highly active against Aspergillus terreus 6.01 ± 0.200. Bioactive compounds of C. sativum and M. azedarach were assayed for in vitro antibacterial activity against Staphylococcus aureus, Proteus mirabilis, Pseudomonas aerogenosa, Escherichia coli and Klebsiella pneumonia using the diffusion method in agar. The zone of inhibition was compared with different standard antibiotics. The diameters of inhibition zones ranged from 5.60 ± 0.320 to 1.96 ± 0.200 mm for all treatments.
Twenty five bioactive phytochemical compounds were identified in the methanolic extract of Equise... more Twenty five bioactive phytochemical compounds were identified in the methanolic extract of Equisetum arvense and Alchemila valgaris. The identification of phytochemical compounds is based on the peak area, retention time molecular weight and molecular formula. GC/MS analysis of Equisetum arvense revealed the existence of the Cyclohexene, 4
The aim of this study was of twofold. One was to determine the genetic structure of Iraq populati... more The aim of this study was of twofold. One was to determine the genetic structure of Iraq population and the second objective of the study was to evaluate the importance of these loci for forensic genetic purposes. FTA® Technology (FTA™ paper DNA extraction) utilized to extract DNA. Twenty (20) STR loci and Amelogenin), including D3S1358, D13S317, and Amelogenin amplified by using power plex21® kit. Polymerase chain reaction (PCR) products detected by genetic analyzer 3730xL then data analyzed by PowerStatsV1.2. Based on the allelic frequencies, several statistical parameters of genetic and forensic efficiency have been estimated. This includes the homozygosity and heterozygosity, effective number of alleles (n), the polymorphism information content (PIC), the power of discrimination (DP) and the power of exclusion (PE). The power of discrimination values for all tested loci was from 75 to 96%; therefore, those loci can be safely used to establish a DNA-based database for Iraq population. The high PIC values of the selected markers confirm their usefulness for genetic polymorphism studies and linkage mapping programs in human as well. The mean heterozygosity observed, is expected to have mean PIC values across the 20 loci which were 0.77, 0.81 and 0.78, respectively, indicating high gene diversity.
The main objective of this study was to determine the phytochemical composition in the leaves of
... more The main objective of this study was to determine the phytochemical composition in the leaves of Coriandrum sativum, using methanolic extraction and report the main functional components by using IR technique. The phytochemical compounds in the extract were then screened by GC-MS method. Seven bioactive phytochemical compounds were identified in the methanolic extract of C. sativum: 1,6- octadien-3-ol, 3,7-dimethyl, 1,6-octadien-3-ol,3,7-dimethyl, 2-aminobenzoate, bicyclo[2.2.1]heptan-2- one,1,7,7-trimethyl., geranyl vinyl ether, 9,10-secocholesta-5,7,10(19)-triene-3,24,25-triol., ascorbic acid 2,6-dihexadecanoate and 7aH-cyclopenta[a] cyclopropa[f]cycloundecene. Thirteen bioactive phytochemical compounds were identified in the methanolic extract of Melia azedarach. In the present investigation, a variety of compounds have been detected in M. azedarach including trichloromethane, propanedioic acid, diethyl ester, 2-pyrrolidinyl-methylamine, butanedioic acid, diethyl ester, 2- piperidimethanamine, butanedioic acid, hydroxyl-, diethyl ester, 2,5-dimethylhexane-2,5- dihydroperoxide, dithiocarbamate, s-methyl-,n-(2-methyl-3-oxobutyl), triethyl citrate, y-sitosterol, ethyl 9,12,15-octadecatrienoate, hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, and octadecane, 3-ethyl-5-(2-ethylbutyl). It contains chemical constitutions which may be useful for various herbal formulation as anti-inflammatory, analgesic, antipyretic, cardiac tonic and antiasthamatic. C. sativum is highly active against Aspergillus terreus 6.01 ± 0.200. Bioactive compounds of C. sativum and M. azedarach were assayed for in vitro antibacterial activity against Staphylococcus aureus, Proteus mirabilis, Pseudomonas aerogenosa, Escherichia coli and Klebsiella pneumonia using the diffusion method in agar. The zone of inhibition was compared with different standard antibiotics. The diameters of inhibition zones ranged from 5.60 ± 0.320 to 1.96 ± 0.200 mm for all treatments.
Twenty five bioactive phytochemical compounds were identified in the methanolic extract of Equise... more Twenty five bioactive phytochemical compounds were identified in the methanolic extract of Equisetum arvense and Alchemila valgaris. The identification of phytochemical compounds is based on the peak area, retention time molecular weight and molecular formula. GC/MS analysis of Equisetum arvense revealed the existence of the Cyclohexene, 4
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Papers by Imad Hadi
Coriandrum sativum, using methanolic extraction and report the main functional components by using
IR technique. The phytochemical compounds in the extract were then screened by GC-MS method.
Seven bioactive phytochemical compounds were identified in the methanolic extract of C. sativum: 1,6-
octadien-3-ol, 3,7-dimethyl, 1,6-octadien-3-ol,3,7-dimethyl, 2-aminobenzoate, bicyclo[2.2.1]heptan-2-
one,1,7,7-trimethyl., geranyl vinyl ether, 9,10-secocholesta-5,7,10(19)-triene-3,24,25-triol., ascorbic acid
2,6-dihexadecanoate and 7aH-cyclopenta[a] cyclopropa[f]cycloundecene. Thirteen bioactive
phytochemical compounds were identified in the methanolic extract of Melia azedarach. In the present
investigation, a variety of compounds have been detected in M. azedarach including trichloromethane,
propanedioic acid, diethyl ester, 2-pyrrolidinyl-methylamine, butanedioic acid, diethyl ester, 2-
piperidimethanamine, butanedioic acid, hydroxyl-, diethyl ester, 2,5-dimethylhexane-2,5-
dihydroperoxide, dithiocarbamate, s-methyl-,n-(2-methyl-3-oxobutyl), triethyl citrate, y-sitosterol, ethyl
9,12,15-octadecatrienoate, hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, and octadecane,
3-ethyl-5-(2-ethylbutyl). It contains chemical constitutions which may be useful for various herbal
formulation as anti-inflammatory, analgesic, antipyretic, cardiac tonic and antiasthamatic. C. sativum is
highly active against Aspergillus terreus 6.01 ± 0.200. Bioactive compounds of C. sativum and M.
azedarach were assayed for in vitro antibacterial activity against Staphylococcus aureus, Proteus
mirabilis, Pseudomonas aerogenosa, Escherichia coli and Klebsiella pneumonia using the diffusion
method in agar. The zone of inhibition was compared with different standard antibiotics. The diameters
of inhibition zones ranged from 5.60 ± 0.320 to 1.96 ± 0.200 mm for all treatments.
Coriandrum sativum, using methanolic extraction and report the main functional components by using
IR technique. The phytochemical compounds in the extract were then screened by GC-MS method.
Seven bioactive phytochemical compounds were identified in the methanolic extract of C. sativum: 1,6-
octadien-3-ol, 3,7-dimethyl, 1,6-octadien-3-ol,3,7-dimethyl, 2-aminobenzoate, bicyclo[2.2.1]heptan-2-
one,1,7,7-trimethyl., geranyl vinyl ether, 9,10-secocholesta-5,7,10(19)-triene-3,24,25-triol., ascorbic acid
2,6-dihexadecanoate and 7aH-cyclopenta[a] cyclopropa[f]cycloundecene. Thirteen bioactive
phytochemical compounds were identified in the methanolic extract of Melia azedarach. In the present
investigation, a variety of compounds have been detected in M. azedarach including trichloromethane,
propanedioic acid, diethyl ester, 2-pyrrolidinyl-methylamine, butanedioic acid, diethyl ester, 2-
piperidimethanamine, butanedioic acid, hydroxyl-, diethyl ester, 2,5-dimethylhexane-2,5-
dihydroperoxide, dithiocarbamate, s-methyl-,n-(2-methyl-3-oxobutyl), triethyl citrate, y-sitosterol, ethyl
9,12,15-octadecatrienoate, hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, and octadecane,
3-ethyl-5-(2-ethylbutyl). It contains chemical constitutions which may be useful for various herbal
formulation as anti-inflammatory, analgesic, antipyretic, cardiac tonic and antiasthamatic. C. sativum is
highly active against Aspergillus terreus 6.01 ± 0.200. Bioactive compounds of C. sativum and M.
azedarach were assayed for in vitro antibacterial activity against Staphylococcus aureus, Proteus
mirabilis, Pseudomonas aerogenosa, Escherichia coli and Klebsiella pneumonia using the diffusion
method in agar. The zone of inhibition was compared with different standard antibiotics. The diameters
of inhibition zones ranged from 5.60 ± 0.320 to 1.96 ± 0.200 mm for all treatments.