Papers by Catiana Zampini
Natural product communications
The effect of seasonality on Argentine propolis collected during one year on its phenolic and fla... more The effect of seasonality on Argentine propolis collected during one year on its phenolic and flavonoid content and on the growth of Gram-positive and Gram-negative antibiotic resistant bacteria and Candida species was evaluated. Extracts of propolis samples collected in the summer and spring showed higher phenolic and flavonoid contents than the samples collected in other seasons (5.86 to 6.06 mg GAE/mL and 3.77 to 4.23 mg QE/mL, respectively). The propolis collected in summer and autumn showed higher antibacterial activity (30 microg/mL) than the other samples (MIC values between 30 and 120 microg/mL). No antibacterial activity was detected against Gram-negative bacteria. Also, these extracts were able to inhibit the development of five Candida species, with MFC values of 15-120 microg/mL. Pharmaceutical formulations containing the more active propolis extract were prepared. The hydrogel of acrylic acid polymer containing summer propolis extract as an antimicrobial agent showed mi...
Actividad de extractos alcohólicos de Nardophyllum armatum (Wedd.) Reiche (Asteraceae) de dos áre... more Actividad de extractos alcohólicos de Nardophyllum armatum (Wedd.) Reiche (Asteraceae) de dos áreas geográficas de la Puna argentina sobre microorganismos fitopatógenos R e s u m e n — D'Almeida, Romina E.; Alberto, María R.; Zampini, Iris C.; Ordoñez Roxa-na M.; Isla, María I. 2011. "Actividad de extractos alcohólicos de Nardophyllum armatum (Wedd.) Reiche (Asteraceae) de dos áreas geográficas de la Puna argentina sobre microor-ganismos fitopatógenos". Lilloa 48 (1). El objetivo del presente trabajo fue evaluar la capaci-dad antibacteriana y antifúngica de extractos de Nardophyllum armatum (Wedd.) Reiche (As-teraceae) de diferentes áreas geográficas de la Puna Argentina sobre microorganismos fito-patógenos de interés en la industria agrícola. Ambos extractos fueron eficientes para inhibir el crecimiento de hongos miceliales fitopatógenos tales como Penicillium digitatum y Geotri-chum candidum por ensayos de macrodilución en agar. A 96 h de incubación el crecimiento de...
Plant Foods for Human Nutrition, 2015
Copao (Eulychnia acida Phil., Cactaceae) is an endemic species occurring in northern Chile. The e... more Copao (Eulychnia acida Phil., Cactaceae) is an endemic species occurring in northern Chile. The edible fruits of this plant are valued for its acidic and refreshing taste. Phenolic-enriched extracts from copao fruit pulp and epicarp, collected in the Elqui and Limari river valleys, were assessed by its in vitro ability to inhibit the pro-inflammatory enzymes lipoxygenase (LOX) and cyclooxygenases (COX-1 and COX-2). At 100 μg/mL, pulp extracts showed better effect towards LOX than epicarp extract, while COX-2 inhibition was observed for both epicarp and pulp samples. In general, the extracts were inactive towards COX-1. A positive correlation was observed between the anti-inflammatory activity and the main phenolic compounds found in this fruit. Copao fruits from the Limari valley, a main place of collection and commercialization, showed major activity, adding evidence on the possible health-beneficial effects of this native Chilean fruit.
LWT - Food Science and Technology, 2011
... Author , Ana Craig a , Roxana Ordoñez a , b , Catiana Zampini a , b , Jorge Sayago a , b , En... more ... Author , Ana Craig a , Roxana Ordoñez a , b , Catiana Zampini a , b , Jorge Sayago a , b , Enrique Bedascarrasbure c , Alejandro Alvarez c , Virginia Salomón c ... A method described by Popova, Silici, Kaftanoglu, and Bankova (2005) was used for total flavonoids determination. ...
Natural Product Research, 2013
Hydroalcoholic and aqueous extracts of some climber species from the Bignoniaceae family that gro... more Hydroalcoholic and aqueous extracts of some climber species from the Bignoniaceae family that grow in the north of Argentina were evaluated for in vitro antibacterial activity against Gram-positive and Gram-negative strains. By means of bioautography and disc diffusion methods, it could be determined that all infusions were not active, whereas the hydroalcoholic extracts of seven species were able to inhibit bacterial growth. The minimum inhibitory concentration and minimum bactericidal concentration observed were between 62.5 and 1000 μg gallic acid equivalent (GAE)/mL and between 125 and 1000 μg GAE/mL, respectively. The tested extracts were more active against Gram-positive microorganisms. Time-kill experiments indicated that all extracts have bacteriostatic activity. Phytochemical screening showed the presence of terpenoids, phenols and flavonoids. The amount of phenolic compounds and flavonoids was higher in tinctures when compared with infusions. These results suggest the presence of antibacterial substances in the hydroalcoholic extracts, which could be used for the treatment of infections.
Journal of the Science of Food and Agriculture, 2012
BACKGROUND: In the last few years, agricultural uses of waste waters from olive processing-relate... more BACKGROUND: In the last few years, agricultural uses of waste waters from olive processing-related industries have been gaining interest mainly with a view to composting or bio-fertilizers. The present work examines physico-chemical, toxicological and geno-toxicological properties of three liquid wastes, namely olive mill wastewater (OMWW), olive wet husk and olive brine. The effect of OMWW spreading on soil microbial activity and biomass was also evaluated.
Journal of the Chilean Chemical Society, 2009
In the traditional medicine of the South American highlands, the infusion and decoction of aerial... more In the traditional medicine of the South American highlands, the infusion and decoction of aerial parts of "tola", Baccharis incarum (Wedd.) Perkins (Asteraceae) is orally taken as an antiseptic, antipyretic and digestive or externally applied to relieve pain and inflammation.
Journal of Hazardous Materials, 2008
Assessment of water pollution and its effect upon river biotic communities and human health is in... more Assessment of water pollution and its effect upon river biotic communities and human health is indispensable to develop control and management strategies. The aim of this work was to ascertain the biotoxicity of water pollution in samples from industrial effluent discharge areas of Tucumán, Argentina by means of biological tests. Chemical characterization of the water pollution was verified by measuring dissolved oxygen concentration or levels of suspended matter and salts. Genotoxic/mutagenic potential was determined using Allium anaphase-telophase and Ames/Salmonella tests. All samples were phytotoxic and genotoxic for Allium roots. Micronucleus and anaphase aberrations were observed, but they did not show mutagenic effects on Salmonella typhimurium, TA98 and TA100 strains with and without metabolic activation (S9). Our results show the importance of testing industrial effluents by chemical methods and complementary biological tests to optimize the control policy on these environmental samples.
Journal of Ethnopharmacology, 2009
a b s t r a c t Aim: The plant species reported here are traditionally used in the "Puna" or "Alt... more a b s t r a c t Aim: The plant species reported here are traditionally used in the "Puna" or "Altiplano" of Argentina for ailments related to bacterial infections. The aim of this study was to evaluate their antimicrobial properties against a panel of sensitive and multi-resistant Gram-positive and Gram-negative bacteria. Materials and methods: The antimicrobial activity of tinctures and aqueous extracts (Baccharis boliviensis, Chiliotrichiopsis keidelii, Chuquiraga atacamensis, Fabiana bryoides, Fabiana densa, Fabiana punensis, Frankenia triandra, Parastrephia lucida, Parastrephia lepidophylla, Parastrephia phyliciformis, Tetraglochin cristatum) was determined using the agar macrodilution and broth microdilution methods recommended by the Clinical and Laboratory Standards Institute (CLSI, formerly NCCLS). The antibiotic resistant clinical strains were isolated from nosocomial infection in human lesions of skin and soft parts. Results: The ethanolic extracts of 11 plant species inhibited the growth of one or more of the following strains: Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Enterobacter cloacae, Morganella morganii, Pseudomonas aeruginosa. Ethanol extracts (tinctures) of aerial parts of Baccharis, Fabiana and Parastrephia showed the highest levels of antibacterial activity on methicillin, oxacillin and gentamicin resistant Staphylococcus with MIC values from 20 to 150 g/ml.
Journal of Agricultural and Food Chemistry, 2010
Cyphomandra betacea ripe fruits can be a source of value-added byproducts and products such as an... more Cyphomandra betacea ripe fruits can be a source of value-added byproducts and products such as antioxidant supplements, ingredients for food processing or alternative medical products. The aims of the present study were to obtain different preparations of C. betacea fruits, such as juice, decoction, and maceration and to characterize them in terms of microbiological stability, sensorial and chemical parameters, antioxidant potential (DPPH and ABTS*+ radical scavenging, beta-carotene bleaching, nitrite scavenging activities), capacity to prevent oxidative stress-induced cell death, and genotoxicity. The best antioxidant activity was found in C. betacea fruit maceration, probably as a consequence of the high flavonoid and anthocyanin content. Nevertheless, all preparations analyzed proved to be good as free radical scavengers (SC50 values between 1.88 and 44 microg/mL) and exerted protection against beta-carotene oxidation. Total phenolic compounds and flavonoids showed a better correlation than anthocyanins with the free radical scavenging effect of the assayed foods. The insoluble matters (pomace) obtained after juice preparation showed antioxidant activity by quenching free radicals. Furthermore, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) reduction assay showed that C. betacea preparations prevent oxidative stress-induced cell death in HepG2 cells in a dose-dependent manner. Salmonella microsome assays show no mutagenic effect. The data presented in this study demonstrate that C. betacea ripe fruits, aqueous and ethanolic preparations, and pomace may be a good source of antioxidant compounds in nutraceutical or functional-food products.
Food Research International, 2010
Fruits such as Prosopis pod have been food sources (patay, arrope, chicha or aloja) of inhabitant... more Fruits such as Prosopis pod have been food sources (patay, arrope, chicha or aloja) of inhabitants of arid and semi-arid lands in South America. The aims of the present study were determine some nutritional and functional properties as well as genotoxicity of flour obtained from Prosopis ripe pods that were submitted to different processing. Sucrose constituted the main sugar for flours obtained from Prosopis alba and Prosopis nigra. Decoctions and macerations showed around 2.9% and 1.4% of soluble proteins, respectively. The highest free phenolics, flavonoids and condensed tannins contents were observed in aqueous extractions with heating. None of the samples presented phytic acid levels high enough to constitute a nutritional problem. Antioxidant activity (AA) was evaluated by DPPH, ABTS and b-carotene bleaching assays. Results showed that the antioxidant potential was significantly higher in flour obtained from P. nigra pods than in that from P. alba pods, and it was also higher in aqueous extracts than in alcoholic ones. Data obtained suggests that compounds responsible for AA are thermostable; therefore, Prosopis flour might be capable of retaining a significant amount of antioxidant capacity after heating. Prosopis extracts did not show any mutagenic effect with and without metabolic activation. Prosopis flour proved to be a non conventional, novel and rich source of antioxidant compounds that could help to prevent pathologies associated with oxidative stress.
LWT-Food Science and …, 2011
... Author , Ana Craig a , Roxana Ordoñez a , b , Catiana Zampini a , b , Jorge Sayago a , b , En... more ... Author , Ana Craig a , Roxana Ordoñez a , b , Catiana Zampini a , b , Jorge Sayago a , b , Enrique Bedascarrasbure c , Alejandro Alvarez c , Virginia Salomón c ... A method described by Popova, Silici, Kaftanoglu, and Bankova (2005) was used for total flavonoids determination. ...
El objetivo de este trabajo fue realizar un screening fitoquímico y biológico de extractos alcohó... more El objetivo de este trabajo fue realizar un screening fitoquímico y biológico de extractos alcohólicos y acuosos de Nardophyllum armatum Wedd o Dolichogyne armata Wedd (Asteraceae), recolectada a diferentes niveles altitudinales (3650 y 4200 msnm). Se demostró mediante técnicas ...
Boletín Latinoamericano y …
El objetivo de este trabajo fue realizar un estudio comparativo de la capacidad antioxidante de e... more El objetivo de este trabajo fue realizar un estudio comparativo de la capacidad antioxidante de extractos de Baccharis incarum obtenidos a través de diferentes técnicas de extracción a presión normal ya presiones criticas (Pc) comprendidas entre 85 a 95 atm. Se realizaron ...
Food Chemistry, 2015
Geoffroea decorticans (chañar), is widely distributed throughout Northwestern Argentina. Its frui... more Geoffroea decorticans (chañar), is widely distributed throughout Northwestern Argentina. Its fruit is consumed as flour, arrope or hydroalcoholic beverage. The chañar fruits flour was obtained and 39 phenolic compounds were tentatively identified by HPLC-MS/MS n . The compounds comprised caffeic acid glycosides, simple phenolics (protocatechuic acid and vanillic acid), a glycoside of vanillic acid, p-coumaric acid and its phenethyl ester as well as free and glycosylated flavonoids. The polyphenols enriched extract with and without gastroduodenal digestion inhibited enzymes associated with metabolic syndrome, including a-amylase, a-glucosidase, lipase and hydroxyl methyl glutaryl CoA reductase. The polyphenolic extract exhibited antioxidant activity by different mechanisms and inhibited the pro-inflammatory enzymes (ciclooxygenase, lipoxygenase and phospholipase A 2 ). The polyphenolic extract did not showed mutagenic effect by Ames test against Salmonella typhimurium TA98 and TA100 strains.
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Papers by Catiana Zampini