Papers by Isabelle Perraud Gaime
Journal of the International Society of Antioxidants in Nutrition & Health, 2016
Crude extracts of Pleurotus spp were tested for their capacity to degrade/modify coffee pulp poly... more Crude extracts of Pleurotus spp were tested for their capacity to degrade/modify coffee pulp polyphenols as well as to produce laccase during colonization, using solid state fermentation. All strains presented similar profiles of laccase production. Coffee pulp showed a reduction in phenolic content during the first two days of incubation. P.ostreatus CCEBI 3023 strain was able to transform 53% of total phenols after 24 h while P. ostreatus CCEBI 3024 strain transformed 45%, after 48 h. All strains tested were able to transform Procyanidin B2 to a great extent evaluated by HPLC. The antioxidant properties of the laccase fraction were determined by the DPPH reduction rate and the inhibition of lipid peroxidation. The antibacterial activity against strains of clinical interest was also evaluated. Until we know, this is the first report with Pleurotus laccases on “the double-edged sword” (pro-oxidative and antioxidant effects). The preliminary results obtained point out to the free rad...
Morocco is a country which produces olive oil extensively and this industry within the country is... more Morocco is a country which produces olive oil extensively and this industry within the country is currently under huge expansion. The olive mill wastewater OMW which is being dumped in the environment results in serious environmental pollution while the olive cake does have a proper disposaI. In the present work, a new integrated process is described to eliminate the OMW produced bya tri-phase system using the technique of pressing. This process consists of mixing olive cake with the OMW produced and then to dry the mixture in a greenhouse to get a final moisture content of 10% (w/w). The dried mixture was characterized by elemental analysis and spectroscopie methods (Fourier transform Infra-Red: FTIR and Nuclear Magnetic Resonance: 13C-NMR), in order to compare it with the crude olive cake and predict its future biotechnological valorization in the way existing as composting, lombricomposting and edible mushroom culture. The extractable polyphenols dynamics were determined by HPLC ...
The results of the cultivation of six strains of Pleurotus spp. (five P. ostreatus and one P. saj... more The results of the cultivation of six strains of Pleurotus spp. (five P. ostreatus and one P. sajor-caju) on coffee pulp by solid state fermentation system a...
Oleiculture and oleology have deeply progressed for these last decades. This progress has notably... more Oleiculture and oleology have deeply progressed for these last decades. This progress has notably induced important changes in ail olive chain levels. Also, in the agronomie level, olive tree culturing is directed towards biologie productions with a minimum of volumes (fertilizers and pesticides); in the technological level, harvests are mechanized and automatized enabling to reduce considerably the time between the harvest and tituration. The generalisation of continuous trituration systems has enabled to treat olives without time out. The conjugation of ail these evolutions has led to virgin olive oils of a high organoleptic quality, on the one hand, and to the obtaining of co-products, in the other hand (margins, pomace, leaves, wood... ) of good quality, enables to direct researches towards biotechnological valorisation of these raw materials. These new products constitute an SOS (Solid Olive Substrate) for the treatment of the margins used as substrate in biotechnology for the ...
Journal of the International Society of Antioxidants in Nutrition & Health, 2016
In this study we determined the total phenolic content as well as the in vitro antioxidant activi... more In this study we determined the total phenolic content as well as the in vitro antioxidant activity of extracts from fruiting bodies of Pleurotus sp obtained at low (LT-E) and high temperatures (HT-E). The heat treatment of cellular biomass favored the releasing of phenolic compounds with values of 16 and 58 μg/mg, for LT-E and HT-E, respectively. The results in the DPPH assay indicated that the percentage of inhibition for LT-E at concentrations between 0.75 and 3 mg/mL was not statistically significant compared to the decoction (HT-E) at 1 mg/mL (p<0.05). However, with respect to the ABTS assay, the LT-E at 0.15 and 3 mg/mL showed better results than the decoction as judged by the radical-scavenging ability. In the cytoprotective evaluation, the LT-E displayed a hemolytic activity and hemoglobin denaturation index of 39.9% and 81, 96%, respectively while the decoction HT-E showed values of 16.9% and 128%, respectively. Both extracts modulated favorably the cytoxicity exerted by...
Despite the worldwide effort to generate ethanol from lignocellulose, there is still no an optima... more Despite the worldwide effort to generate ethanol from lignocellulose, there is still no an optimal process. More research is needed in order to optimize the bioethanol production from organic matter (OM) wastes. The aim of this research was to evaluate the ethanol production process from sugarcane bagasse integrating the chemical pretreatment of OM; the enzyme hydrolysis (saccharification) and fermentation simultaneously (FSS), using Zymomonas mobilis and Saccharomyces cerevisiae immobilized in chitosanalginate beads. Sugarcane bagasse was treated with NaOH (2 % w/v) at 121 °C and 1.1 kg/cm at four different times (15, 30, 60, 120 and 240 min). After this chemical treatment, the bagasse solution was diluted with water (1:3, v/v) and cellulases (2.9 UI mL) from Trichoderma reesei were added to the batch reactor (5L). Saccharification was performed at 50°C, pH 4.9 and at 110 rpm. At the highest reducing sugars concentration, the bioreactor was adjusted at 50°C and the immobilized micr...
ChemistrySelect
HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific re... more HAL is a multidisciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.
Coffee Biotechnology and Quality, 2000
Micro-organisms are unique « cell factories » able to valorize agricultural by-products instead o... more Micro-organisms are unique « cell factories » able to valorize agricultural by-products instead of only degrading or mineralizing them for depollution or methanisation processes. The difficulties encountered in treating such compounds often result from high concentration of pollutants and/or high toxicity to the microflora. Liquid effluents with high chemical oxygen demand (COD) (> 100 g COD/litre) are often rich in aromatic compounds.
… : proceedings of the …, 2000
... Association Scientifique Internationale du Cafe (ASIC), 15eme Colloque, Montpellier, France D... more ... Association Scientifique Internationale du Cafe (ASIC), 15eme Colloque, Montpellier, France De Rozo M P., Velez J., Garcia L A.(1985) Efecto de ... D.(1985), Planta productora de hongos comestibles sobre pulpa de cafe\ Ciencia y Desarollo, 65: 41-48 Hakil M., Denis S., Viniegra ...
Waste and Biomass Valorization
Environmental Science and Pollution Research
The creation of pit lakes is usually an acceptable solution from the landscaping point of view fo... more The creation of pit lakes is usually an acceptable solution from the landscaping point of view for voids left by discontinued open-pit mines. However, without rehabilitation, these voids represent a potential environmental risk. The aim of the present work was to assess, for the first time, the water quality i.e. physico-chemical characteristics, metal and metalloid (MM) content and ecotoxicity of the waters of the El Cobre Blue Lagoon, a pit lake formed in an open-pit copper mine in Cuba. Potential effects of rainy season vs. dry season and spatial location (different depths) on water characteristics were considered. Results revealed that water contained was moderately acidic (pH = 4.6±0.2), with high electrical conductivity (EC = 3.02±0.03 mS.cm-1), whatever the season. Dissolved oxygen (DO = 9.9±2.0 mg.L-1), Total Dissolved Solid (TDS = 7003±245 mg.L-1) and sulfate concentration (6556±1410 mg.L-1) in the El Cobre Blue Lagoon water were above acceptable limits for sources of surface water as recommended by Cuban standard (NC 1021:2014). High copper (43.6±1.7 mg.L-1) and manganese (24.1±1.1 mg.L-1) contents were detected. Except for EC, sulfates, chlorides, TDS, nitrates and phosphates, other physico-chemical parameters were stable between dry and rainy seasons (p<0.05). El Cobre Blue Lagoon waters showed an ecotoxicological impact on Vibrio fischeri. No significant differences were detected between all sampling points in the lake for each parameter monitored for a given time. These first results show the spatial homogeneity but poor quality of waters from El Cobre Blue lagoon. Remediation processes need to be implemented in order to lessen the human and environmental health risk and favor potential water reuse. We suggest the use of constructed wetlands for water treatment. This preliminary research work can serve to alert Cuban local public authorities to the need to rehabilitate such sites.
Applied Microbiology and Biotechnology, 2016
The use of synthetic dyes for laccase induction in vivo has been scarcely explored. We characteri... more The use of synthetic dyes for laccase induction in vivo has been scarcely explored. We characterized the effect of adding different synthetic dyes to liquid cultures of Pycnoporus sanguineus on laccase production. We found that carminic acid (CA) can induce 722 % and alizarin yellow 317 % more laccase than control does, and they promoted better fungal biomass development in liquid cultures. Aniline blue and crystal violet did not show such positive effect. CA and alizarin yellow were degraded up to 95 % during P. sanguineus culturing (12 days). With this basis, CA was selected as the best inducer and used to evaluate the induction of laccase on solid-state fermentation (SSF), using sugarcane bagasse (SCB) as substrate, in an attempt to reach selective delignification. We found that laccase induction occurred in SSF, and a slight inhibition of cellulase production was observed when CA was added to the substrate; also, a transformation of SCB under SSF was followed by the 13 C cross polarization magic angle spinning (CPMAS) solid-state nuclear magnetic resonance (NMR). Results showed that P. sanguineus can selectively delignify SCB, decreasing aromatic C compounds by 32.67 % in 16 days; O-alkyl C region (polysaccharides) was degraded less than 2 %; delignification values were not correlated with laccase activities. Cellulosecrystallinity index was increased by 27.24 % in absence of CA and 15.94 % when 0.01 mM of CA was added to SCB; this dye also inhibits the production of fungal biomass in SSF (measured as alkyl C gain). We conclude that CA is a good inducer of laccase in liquid media, and that P. sanguineus is a fungus with high potential for biomass delignification.
Conidia of the entomopathogenic fungus Isaria fumosorosea are used to control insect pests in cro... more Conidia of the entomopathogenic fungus Isaria fumosorosea are used to control insect pests in crops. Commercially available mycoinsecticides manufactured with this fungus are produced on a large scale via solid-state cultures (SSC). In order to favour gaseous exchange in SCC, texturizers can be added to increase porosity fraction (ε). This work presents results of water hyacinth (Eichhornia crassipes) as a novel texturizer. A mixture of parboiled rice (PR), with a ε=0.23, was used as a substrate, which was then mixed with water hyacinth (WH amendment) as a texturizer at different proportions affecting ε. Strains CNRCB1 and ARSEF3302 of I. fumosorosea yielded 1.6 (1.49–1.71)×109 and 7.3 (7.02–7.58)×109 conidia per gram of initial dry rice after 8 days, at ε values of 0.34 and 0.36, respectively. Improvement of conidial yields corresponded to 1.33 and 1.55 times, respectively, compared to rice alone using WH amendment in the mixtures PR:WH (%) at 90–10 and 80–20. In addition, infectivity against Galleria mellonella larvae was maintained. This is the first report of the use of water hyacinth as a texturizer in SSC, affecting ε, which is proposed a key parameter in conidia production by I. fumosorosea, without affecting conidial infectivity.
Electronic Journal of Biotechnology, 2015
ABSTRACT Background: Ethanol has been pointed out as a laccase inducer. However, there are contro... more ABSTRACT Background: Ethanol has been pointed out as a laccase inducer. However, there are controversial reports about its efficiency with some fungi. In this study, we hypothesized that ethanol laccase induced in Pycnoporus sanguineus depends on nitrogen nutriment conditions. To prove this, we assessed laccase production in submerged cultures of P. sanguineus, with different nitrogen concentrations and with, or without ethanol added in a factorial designed experiment. Results: In order to analyze the effects of factors on the response variables, a factorial ANOVA, and response-surface models were performed. It was found that the nitrogen source was the main factor that affected laccase production in P. sanguineus. The treatments with yeast extract (2 g/L) and ethanol (3 g/L) induced the highest laccase activity (31.01 ± 4.9 U/L), while the treatments with urea reached the lowest activity (less than 1.6 U/L). Ethanol had positive and synergic effects on laccase production, in accordance with the surface response model, as long as simple nitrogen sources (urea) were not available. Conclusions: We suggest that laccase in P. sanguineus is regulated by a catabolic nitrogen repression mechanism; laccase activity is strongly inhibited by urea used as nitrogen source and it decreases when the amount of urea increases; contrarily, a synergic positive effect was observed between yeast extract and ethanol on laccase production.
In Morocco, the olive industry produces great amounts of olive mill wastewater (OMW) yearly in a ... more In Morocco, the olive industry produces great amounts of olive mill wastewater (OMW) yearly in a short period (250 000 m3 of liquid wastes in four months, November–February). Phenolic compounds are largely responsible for the phytotoxicity and antimicrobial effects of OMW. Several studies have been carried out on biological and enzymatic treatments of OMW. However, the use of OMW to produce value-added
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Papers by Isabelle Perraud Gaime