Poly(acryloyl-L-proline methyl ester)-based hydrogels containing 1 and 5% of a crosslinking agent... more Poly(acryloyl-L-proline methyl ester)-based hydrogels containing 1 and 5% of a crosslinking agent were studied as drug delivery systems. The drug loading properties were investigated by matrix incubation into solutions containing biomolecules with molecular weight ranging between 300 and 65 000 Da. The loading yield was found to depend on both the crosslinking degree and the molecular weight of the drug. In vitro release studies were carried out with both swollen and dry matrices loaded with gentamicin, isoniazid and insulin. Gentamicin and isoniazid were released by a bimodal Fickian diffusion with a remarkable burst that was found to depend on both matrix crosslinking degree and physical state. In vivo, the subcutaneous implantation into mice of the isoniazid loaded matrices allowed for an efficient drug release for 800 h. In vitro insulin was released from the swollen matrices for 1500 h by diffusional Fickian mechanism while the dry ones displayed a lag time followed by Fickian diffusion release. The subcutaneous implantation of the insulin-loaded matrices into diabetic mice induced a remarkable decrease in the glucose concentrations in blood. In particular, the dry 1% matrices were found to maintain a low glucose level for 700 h.
European Food Research and Technology, Nov 30, 2021
Grape pomace is a winery by-product that is more and more valorized as a source of healthy bioact... more Grape pomace is a winery by-product that is more and more valorized as a source of healthy bioactive molecules such as polyphenols. In addition, it can be used to produce some alcoholic beverages, such as grappa , which is a typical distilled Italian alcoholic product. The spent grape pomace after grappa elaboration is mainly considered a food waste. The aim of this study was to reconsider and valorize red and white pomaces obtained after the production of grappa. The total phenolic content of both samples, as well as the antioxidant activity had a decrease after the distillation; however, it was not significant in the case of red pomace. Regarding the phenolic profile, the behavior during the distillation was different, according to the type of pomace. After the grappa production, catechins and epicatechins were the most significant phenolics in white and red pomace, respectively, demonstrating the remaining bioactivity of this by-product, which could be useful within the food industry.
Article type : Research Impact of consumption of cooked red and black Chenopodium quinoa Willd. o... more Article type : Research Impact of consumption of cooked red and black Chenopodium quinoa Willd. over blood lipids, oxidative stress, and blood glucose levels in hypertension-induced rats
The present study was designed to investigate the antioxidant and antimicrobial activities of pre... more The present study was designed to investigate the antioxidant and antimicrobial activities of pressurized liquid extracts from Haberlea rhodopensis Friv. The total phenolic content was performed using the Folin-Ciocalteu phenol reagent. To determine the antioxidant activities of the extracts, several complementary tests were used: ABTS and DPPH radical scavenging activities, oxygen radical absorbance capacity, and ferric-reducing antioxidant power assay. The phenolic concentration was 15.98±0.09 and 9.42±0.06 mg GAE g-1 DW for 70 and 85% ethanol extracts, respectively. Of all the performed methods, the highest antioxidant activity values were measured by the ORAC assay-224.6±6.6 and 154.0±9.9 μM TE g-1 DW for 70 and 85% ethanol extracts, respectively. Results also showed that both extracts exhibited very weak antimicrobial activity against the examined microorganisms. However, the 70% ethanol extract possessed higher inhibition ability, which correlated with higher total phenolic content and antioxidant activity.
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Environmental Technology and Innovation, May 1, 2023
Sugarcane bagasse is most directly used as fuel; therefore, its economic value is not maximized. ... more Sugarcane bagasse is most directly used as fuel; therefore, its economic value is not maximized. Steam explosion and ultrasound-assisted extraction (UAE) was investigated to develop an effective process for the production of valuable phenolic compounds from sugarcane bagasse lignin. The influence of steam explosion (temperatures: 190, 200 and 210°C, and times: 5, 10, 15 and 20 min) as well as UAE (solvent types: 95% ethanol, 70% ethanol, distilled water and ethyl acetate, and conditionspowers: 20, 40 and 60 W, and times: 3, 6 and 9 min) was explored. The yield, total phenolic content (TPC), and trolox equivalent antioxidant capacity (TEAC) of the extract increased with increasing temperature and time, but beyond 10 min at 210°C, a negative impact was observed. The optimal UAE (40 W and 6 min) using 95% ethanol gave the highest TPC (29.11 mg gallic acid equivalent/g), the highest TEAC (82.54 mg TE/g), and the lowest minimal inhibitory concentration (1.77 mg/mL) against Staphylococcus aureus TISTR029 and (7.10 mg/mL) against Escherichia coli O157:H7. The free radical scavenging activity of the extract was comparable to that of ascorbic acid. Reversed-phase high-performance liquid chromatography analysis revealed the presence of gallic acid, hydroxybenzoic acid, vanillic acid, p-coumaric acid, ferulic acid, syringic acid, and sinapic acid. The advantage of UAE over maceration was 10 times shorter time. The results indicated that the phenolic compounds from sugarcane bagasse lignin had a potential use as a natural antioxidant and antibacterial, which caused a great impact on the sugar industry.
enzimatico nel settore agro-alimentare e cosmetico. Inoltre, questi estratti naturali, ricchi in ... more enzimatico nel settore agro-alimentare e cosmetico. Inoltre, questi estratti naturali, ricchi in composti bioattivi con forti proprietà inibitorie, suggeriscono un possibile impiego alternativo che potrebbe conferire un interessante valore aggiunto a questi sottoprodotti della filiera agroindustriale.
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Poly(acryloyl-L-proline methyl ester)-based hydrogels containing 1 and 5% of a crosslinking agent... more Poly(acryloyl-L-proline methyl ester)-based hydrogels containing 1 and 5% of a crosslinking agent were studied as drug delivery systems. The drug loading properties were investigated by matrix incubation into solutions containing biomolecules with molecular weight ranging between 300 and 65 000 Da. The loading yield was found to depend on both the crosslinking degree and the molecular weight of the drug. In vitro release studies were carried out with both swollen and dry matrices loaded with gentamicin, isoniazid and insulin. Gentamicin and isoniazid were released by a bimodal Fickian diffusion with a remarkable burst that was found to depend on both matrix crosslinking degree and physical state. In vivo, the subcutaneous implantation into mice of the isoniazid loaded matrices allowed for an efficient drug release for 800 h. In vitro insulin was released from the swollen matrices for 1500 h by diffusional Fickian mechanism while the dry ones displayed a lag time followed by Fickian diffusion release. The subcutaneous implantation of the insulin-loaded matrices into diabetic mice induced a remarkable decrease in the glucose concentrations in blood. In particular, the dry 1% matrices were found to maintain a low glucose level for 700 h.
European Food Research and Technology, Nov 30, 2021
Grape pomace is a winery by-product that is more and more valorized as a source of healthy bioact... more Grape pomace is a winery by-product that is more and more valorized as a source of healthy bioactive molecules such as polyphenols. In addition, it can be used to produce some alcoholic beverages, such as grappa , which is a typical distilled Italian alcoholic product. The spent grape pomace after grappa elaboration is mainly considered a food waste. The aim of this study was to reconsider and valorize red and white pomaces obtained after the production of grappa. The total phenolic content of both samples, as well as the antioxidant activity had a decrease after the distillation; however, it was not significant in the case of red pomace. Regarding the phenolic profile, the behavior during the distillation was different, according to the type of pomace. After the grappa production, catechins and epicatechins were the most significant phenolics in white and red pomace, respectively, demonstrating the remaining bioactivity of this by-product, which could be useful within the food industry.
Article type : Research Impact of consumption of cooked red and black Chenopodium quinoa Willd. o... more Article type : Research Impact of consumption of cooked red and black Chenopodium quinoa Willd. over blood lipids, oxidative stress, and blood glucose levels in hypertension-induced rats
The present study was designed to investigate the antioxidant and antimicrobial activities of pre... more The present study was designed to investigate the antioxidant and antimicrobial activities of pressurized liquid extracts from Haberlea rhodopensis Friv. The total phenolic content was performed using the Folin-Ciocalteu phenol reagent. To determine the antioxidant activities of the extracts, several complementary tests were used: ABTS and DPPH radical scavenging activities, oxygen radical absorbance capacity, and ferric-reducing antioxidant power assay. The phenolic concentration was 15.98±0.09 and 9.42±0.06 mg GAE g-1 DW for 70 and 85% ethanol extracts, respectively. Of all the performed methods, the highest antioxidant activity values were measured by the ORAC assay-224.6±6.6 and 154.0±9.9 μM TE g-1 DW for 70 and 85% ethanol extracts, respectively. Results also showed that both extracts exhibited very weak antimicrobial activity against the examined microorganisms. However, the 70% ethanol extract possessed higher inhibition ability, which correlated with higher total phenolic content and antioxidant activity.
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Environmental Technology and Innovation, May 1, 2023
Sugarcane bagasse is most directly used as fuel; therefore, its economic value is not maximized. ... more Sugarcane bagasse is most directly used as fuel; therefore, its economic value is not maximized. Steam explosion and ultrasound-assisted extraction (UAE) was investigated to develop an effective process for the production of valuable phenolic compounds from sugarcane bagasse lignin. The influence of steam explosion (temperatures: 190, 200 and 210°C, and times: 5, 10, 15 and 20 min) as well as UAE (solvent types: 95% ethanol, 70% ethanol, distilled water and ethyl acetate, and conditionspowers: 20, 40 and 60 W, and times: 3, 6 and 9 min) was explored. The yield, total phenolic content (TPC), and trolox equivalent antioxidant capacity (TEAC) of the extract increased with increasing temperature and time, but beyond 10 min at 210°C, a negative impact was observed. The optimal UAE (40 W and 6 min) using 95% ethanol gave the highest TPC (29.11 mg gallic acid equivalent/g), the highest TEAC (82.54 mg TE/g), and the lowest minimal inhibitory concentration (1.77 mg/mL) against Staphylococcus aureus TISTR029 and (7.10 mg/mL) against Escherichia coli O157:H7. The free radical scavenging activity of the extract was comparable to that of ascorbic acid. Reversed-phase high-performance liquid chromatography analysis revealed the presence of gallic acid, hydroxybenzoic acid, vanillic acid, p-coumaric acid, ferulic acid, syringic acid, and sinapic acid. The advantage of UAE over maceration was 10 times shorter time. The results indicated that the phenolic compounds from sugarcane bagasse lignin had a potential use as a natural antioxidant and antibacterial, which caused a great impact on the sugar industry.
enzimatico nel settore agro-alimentare e cosmetico. Inoltre, questi estratti naturali, ricchi in ... more enzimatico nel settore agro-alimentare e cosmetico. Inoltre, questi estratti naturali, ricchi in composti bioattivi con forti proprietà inibitorie, suggeriscono un possibile impiego alternativo che potrebbe conferire un interessante valore aggiunto a questi sottoprodotti della filiera agroindustriale.
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
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