A series of experiments were conducted to characterize a novel restorative material. We explored ... more A series of experiments were conducted to characterize a novel restorative material. We explored the effect on biological, physical and chemical properties of glass ionomer cement (GIC) adding-the naturally occurring tt-farnesol (900 mM). Two groups were accomplished for all assays: GIC+tt-farnesol and GIC (control). Biological assays: 1) agar diffusion against some cariogenic bacteria; 2) S. mutans biofilm formation and confocal laser scanning microscopy-CLSM. 3) gtfB, gtfC, gtfD, gbpB, vicR, and covR expression; 4) MTT and microscopic morphology. Physical properties assays: 1) roughness; 2) hardness; 3) compressive strength and 4) diametral tensile strength. Chemical assay: Raman spectroscopy. The adding of tt-farnesol to GIC led to larger zones of inhibition (p<0.05), biofilms with a short-term reduction in bacterial viability but similar biomass (p>0.05). Polysaccharides levels increased over time, similarly over groups (p>0.05). Viable and non-viable S. mutans were seen on the specimens' surface by CLSM but their virulence was not modulated by tt-farne-sol. The tt-farnesol increased the HaCaT cell viability without impact on compressive and diametral tensile strength and roughness although the hardness was positively affected (p<0.05). Raman confirmed the presence of tt-farnesol. The incorporation of tt-farnesol into GIC inhibited the growth of cariogenic bacteria but had a little effect on the composition, structure and physiology of the biofilm matrices. Also, the tt-farnesol increased the hardness and the biocompatibility of the GIC, not influencing negatively other physical properties of the restorative material.
In vitro and in vivo evaluations of glass-ionomer cement containing chlorhexidine for Atraumatic ... more In vitro and in vivo evaluations of glass-ionomer cement containing chlorhexidine for Atraumatic Restorative Treatment Objectives: Addition of chlorhexidine has enhanced the antimicrobial effect of glass ionomer cement (GIC) indicated to Atraumatic Restorative Treatment (ART); however, the impact of this mixture on the properties of these materials and on the longevity of restorations must be investigated. The aim of this study was to evaluate the effects of incorporating chlorhexidine (CHX) in the in vitro biological and chemical-mechanical properties of GIC and in vivo clinical/ microbiological follow-up of the ART with GIC containing or not CHX. Material and Methods: For in vitro studies, groups were divided into GIC, GIC with 1.25% CHX, and GIC with 2.5% CHX. Antimicrobial activity of GIC was analyzed A randomized controlled trial was conducted on 36 children that received ART mutans streptococci (MS) counts and the survival rate of restorations was checked after 7 days, 3 months and one year after ART. ANOVA/Tukey or Kruskal-Wallis/ Mann-Whitney tests were performed for in vitro tests and in vivo microbiological analysis. The Kaplan-Meier method and Log rank tests were applied to estimate survival percentages of restorations (p<0.05). Results: Incorporation of 1.25% affecting F release and mechanical characteristics, but 2.5% CHX was cytotoxic. Survival rate of restorations using GIC with 1.25% CHX was similar to GIC. A 1.25% CHX increased the in vitro antimicrobial activity, without changing chemical-mechanical properties of GIC and odontoblast-like cell viability. This combination improved the in vivo short-term microbiological effect without affecting clinical performance of ART restorations. 2017;25(5):541-50 542 J Appl Oral Sci.
Background: Streptococcus mutans orchestrates the development of a biofilm that causes dental car... more Background: Streptococcus mutans orchestrates the development of a biofilm that causes dental caries in the presence of dietary sucrose, and, in the bloodstream, S. mutans can cause systemic infections. The development of a cariogenic biofilm is dependent on the formation of an extracellular matrix rich in exopolysaccharides, which contains extracellular DNA (eDNA) and lipoteichoic acids (LTAs). While the exopolysaccharides are virulence markers, the involvement of genes linked to eDNA and LTAs metabolism in the pathogenicity of S. mutans remains unclear. Objective and Design: In this study, a parental strain S. mutans UA159 and derivative strains carrying single gene deletions were used to investigate the role of eDNA (ΔlytS and ΔlytT), LTA (ΔdltA and ΔdltD), and insoluble exopolysaccharides (ΔgtfB) in virulence in a rodent model of dental caries (rats) and a systemic infection model (Galleria mellonella larvae). Results: Fewer carious lesions were observed on smooth and sulcal surfaces of enamel and dentin of the rats infected with ΔlytS, ΔdltD, and ΔgtfB (vs. the parental strain). Moreover, strains carrying gene deletions prevented the killing of larvae (vs. the parental strain). Conclusions: Altogether, these findings indicate that inactivation of lytST and dltAD impaired S. mutans cariogenicity and virulence in vivo.
This study evaluated gloss and color changes of esthetic restorative materials subjected to diffe... more This study evaluated gloss and color changes of esthetic restorative materials subjected to different acidic beverages. Specimens of resin composites (Z350XT (Z350), IPS Empress Direct (ED), Charisma Diamond (CD)) were prepared and the initial surface gloss and color (ΔE) were measured (n=10). Then, the specimens were immersed in 4 mL of each of the different beverages (cranberry juice; Coca-Cola; coffee or artificial saliva) during 15 min, 3x/day for 14 days and new gloss and color readings were obtained. Color change was evaluated with the ΔE formula and gloss change values were obtained by the formula: (final gloss-initial gloss). Data was submitted to two-way ANOVA followed by Tukey's post hoc test (α=0.05). CD showed the lowest color change among resin composites. The highest ΔE values were obtained after immersion in coffee and cranberry juice. Coffee promoted the highest gloss change (worst gloss retention), followed by cranberry juice, Coca-cola and artificial saliva (p<0.05). The type of beverage significantly influenced the gloss of resin composites. Coca-cola reduced gloss of the three resin composites in a similar manner. Coffee affected the ED gloss more than that of Z350 and CD, while cranberry juice affected Z350 more than ED and CD. Saliva had a more pronounced effect on the gloss retention of CD than ED. The beverages used in this study influenced the optical surface properties of the composites studied.
Objectives: The present study aimed to assess the antibacterial activity against bacteria with ca... more Objectives: The present study aimed to assess the antibacterial activity against bacteria with cariogenic relevance , toxic and genotoxic potential of the plants Anacardium occidentale L. and Anadenanthera macrocarpa (Benth.) Bernam. Design: Using a microdilution technique, the extracts were submitted to minimum inhibitory concentration (MIC) testing against Streptococcus mitis (ATCC 903), Streptococcus mutans (ATCC 25175), Streptococcus oralis (ATCC 10557), Streptococcus salivarius (ATCC 7073), Streptococcus sanguinis (ATCC 15300) and Streptococcus sobrinus (ATCC 27609). The toxicity of the extracts was then verified against eukaryotic cells. Additionally, a micronucleus assay was performed to investigate the potential mutagenic effects of the extracts on rat ery-throcytes. The Student's t-test, Bonferroni test, and one-way ANOVA followed by Tukey's tests were used for statistical analysis, at a significance level of 5%. Results: While the A. occidentale extract was able to inhibit all of the tested strains, with S. mutans and S. mitis being the most susceptible to that extract́sextract́s action, the A. macrocarpa did not show antimicrobial activity. Interestingly, the hemolytic, oxidant and antioxidant activities were slightly observed for either extract, even at high concentrations (1000 mg/mL). The micronucleus assay showed no significant changes in the cells exposed to the extracts. Conclusion: The A. occidentale extract has potential as an antimicrobial agent with low eukaryotic cell toxicity or mutagenic activity. The A. macrocarpa extract, although absent of antibacterial activity might as well be a safe and effective phytotherapeutic alternative.
www.scielo.br/jaos http://dx. Objective: This in vitro study assessed the anti-erosive effect of ... more www.scielo.br/jaoshttp://dx. Objective: This in vitro study assessed the anti-erosive effect of experimental mouthrinses containing TiF 4 and NaF on dentin erosive loss. Material and Methods: Bovine dentin specimens were randomly allocated into the groups (n=15): 1) SnCl 2 /NaF/AmF (Erosion Protection ® /GABA, pH 4.5, positive control); 2) experimental solution with 0.0815% TiF 4 (pH 2.5); 3) 0.105% NaF (pH 4.5); 4) 0.042% NaF+0.049% TiF 4 (pH 4.4); 5) 0.063% NaF+0.036% TiF 4 (pH 4.5); 6) no treatment (negative control). Each specimen was cyclically demineralied (Sprite ero, pH 2.6, 4x90 s/day) and exposed to articial saliva between the erosive challenges for days. The treatment with the uoride solutions was done 2x60 s/day, immediately after the rst and the last erosive challenges of the day. entin erosive loss was measured by prolometry (m). The data were analyed using rusal allis/ Dunn tests (p<0.05). Results: Mouthrinses containing TiF 4 or Sn/F were able to show some protective effect against dentin erosive loss compared to negative control. The best anti-erosive effect was found for experimental solution containing 0.0815% TiF 4 (100% reduction in dentin loss), followed by 0.042% NaF+0.049% TiF 4 (58.3%), SnCl 2 /NaF/AmF (52%) and 0.063% NaF+0.036% TiF 4 (40%). NaF solution (13.3%) did not signicantly differ from control. Conclusion: The daily application of experimental mouthrinse containing TiF 4 and NaF has the ability to reduce dentin erosion, as well as Erosion Protection ® and TiF 4 alone.
Bonding to demineralized dentin of a diseased tooth has shown to be a significant clinical issue.... more Bonding to demineralized dentin of a diseased tooth has shown to be a significant clinical issue. This study evaluated the effect of 0.2% NaF-(NaF), MI Paste™-(CPP-ACP) and the self-assembling peptide 'P 11−4 ′ (Ace-QQRFEWEFEQQ-NH 2) contained in Curodont™ Repair, have on microtensile bond strength-(µTBS) of two different adhesive systems (Adper™ Single Bond-(SB) or Clearfil™ SE Bond (CSE)) and wettability of deminer-alized dentin slices after remineralising agents were applied. The highest µTBS were found for the demineralized dentin-(DD) treated with CPP-ACP; both adhesives systems (p < 0.05) did not significantly difference from P 11-4 treatment associated with SB, and also presented higher values than sound dentin-(SD/SB) (p < 0.01). DD treated with P 11−4 associated with CSE did not differ from DD/CSE (p > 0.05). The NaF treatment associated with CSE recovered the bond strength values of SD/CSE and associated with CSE demonstrated lower µTBS than other groups, although significantly higher than DD (p < 0.05). P 11−4 and CPP-ACP increased significantly the wettability of demineralized dentin (p < 0.05); etching acid improved wettability for all groups (p < 0.05), whilst NaF did not affect the wettability of demineralized dentin (p > 0.05). Morphological analysis of the dentin surface and dentin-resin interface revealed unique features of the applied remineralizing agent. The results indicated that self-assembling peptide P 11−4 associated with SB and CPP-ACP associated with SB or CSE significantly enhanced the bond strength to demineralized dentin (p < 0.05). We conclude that by modifying the dentine surface and restoring conditions found on sound dentin, this can enhance the interfacial bonding.
In vitro and in vivo evaluations of glass-ionomer cement containing chlorhexidine for Atraumatic ... more In vitro and in vivo evaluations of glass-ionomer cement containing chlorhexidine for Atraumatic Restorative Treatment Objectives: Addition of chlorhexidine has enhanced the antimicrobial effect of glass ionomer cement (GIC) indicated to Atraumatic Restorative Treatment (ART); however, the impact of this mixture on the properties of these materials and on the longevity of restorations must be investigated. The aim of this study was to evaluate the effects of incorporating chlorhexidine (CHX) in the in vitro biological and chemical-mechanical properties of GIC and in vivo clinical/ microbiological follow-up of the ART with GIC containing or not CHX. Material and Methods: For in vitro studies, groups were divided into GIC, GIC with 1.25% CHX, and GIC with 2.5% CHX. Antimicrobial activity of GIC was analyzed A randomized controlled trial was conducted on 36 children that received ART mutans streptococci (MS) counts and the survival rate of restorations was checked after 7 days, 3 months and one year after ART. ANOVA/Tukey or Kruskal-Wallis/ Mann-Whitney tests were performed for in vitro tests and in vivo microbiological analysis. The Kaplan-Meier method and Log rank tests were applied to estimate survival percentages of restorations (p<0.05). Results: Incorporation of 1.25% affecting F release and mechanical characteristics, but 2.5% CHX was cytotoxic. Survival rate of restorations using GIC with 1.25% CHX was similar to GIC. A 1.25% CHX increased the in vitro antimicrobial activity, without changing chemical-mechanical properties of GIC and odontoblast-like cell viability. This combination improved the in vivo short-term microbiological effect without affecting clinical performance of ART restorations. 2017;25(5):541-50 542 J Appl Oral Sci.
The aim of this study was to determine the physicochemical characteristics of leaves of Syzygium ... more The aim of this study was to determine the physicochemical characteristics of leaves of Syzygium cumini L. Skeels plant and characterize the extract of this plant by analytical techniques. Pharmacopeial methods of physicochemical analysis were used, including morphological characterization of the particle, thermal analysis, infrared spectroscopy and X-ray diffraction. The plant drug was presented as a coarse powder, within the appropriate Brazilian Pharmacopoeia parameters. The X-ray diffrac-tion, optical microscopy and scanning electron microscopy suggested that the extract particles are amorphous and have irregular shapes, so that clusters of different sizes and morphologies are displayed. Thermal decomposition of the organic components in the sample started in a step that occurred between 151.64 and 209.27 °C with mass loss of 9.08 %, followed by another step with more significant mass loss (28.16 %). The infrared spectrum, in turn, showed many functional groups of compounds present in the lyophilized extract in different absorption bands. The results showed that the analytical techniques allowed us to characterize the physicochemical properties of the plant leaves, which may be useful in the production of new herbal medicines.
2016): Antifungal potential of Sideroxylon obtusifolium and Syzygium cumini and their mode of act... more 2016): Antifungal potential of Sideroxylon obtusifolium and Syzygium cumini and their mode of action against Candida albicans, Pharmaceutical Biology
4 Doutora em Patologia Bucal pela Faculdade de Odontologia de Bauru da USP e professora do Depart... more 4 Doutora em Patologia Bucal pela Faculdade de Odontologia de Bauru da USP e professora do Departamento de Odontologia da USC. Bauru, SP, Brasil 5 Graduado em Odontologia e aluno de especialização em Bucomaxilo Facial pela Faculdade de Odontologia de Bauru da USC. Bauru, SP, Brasil Resumo Objetivo: Apresentar as características clínicas, trata-mento cirúrgico e achado histológico de um caso de lipoi-doproteinose. Descrição do caso: Criança do sexo masculino, cinco anos de idade, branco, que procurou atendimento odontológico na Uni-versidade. A mãe da criança relatou presença de intensa halitose e dificuldade na alimentação e higienização bucal, decorrentes de crescimento gengival generalizado nos arcos dentários superior e inferior. No exame clínico, verificaram-se comprometimento funcional e estético generalizado (rouquidão, artralgia bilateral no joelho e tornozelo, lesões tumorais nas orelhas, entre outros), além de extensa hiperplasia gengival em ambos os arcos den-tários. Optou...
ABSTRACT The objective of this work was to verify the incidence of dental caries by means of the ... more ABSTRACT The objective of this work was to verify the incidence of dental caries by means of the CPO-D, CPO-S, ceo-d and ceo-s indexes in patients with Down syndrome regularly enrolled in a preventive program. Twenty four Down syndrome patients of both sexes age range of one to 48 years were examined. The prevalence (initial experimental situation) and incidence (final experimental situation) of dental caries were verified using of the initial and final CPO-D, CPO-S, ceo-d and ceo-s indexes of the participants. From 24 individual examined, 10 (42.0%) were free of caries. The prevalence of dental caries showed values of CPO-D= 2.33; CPO-S= 3.60; ceo-d= 1.75 e ceo-s= 2.80; while the incidence of caries showed values of 2.33; 3.80; 1.10 e 1.90, respectively. Down syndrome individuals evaluated in this study presented low level of caries and small incidence of new lesions, emphasizing the importance of the maintenance of these patients at preventive programs.
ABSTRACT The objective of this work was to verify the incidence of dental caries by means of the ... more ABSTRACT The objective of this work was to verify the incidence of dental caries by means of the CPO-D, CPO-S, ceo-d and ceo-s indexes in patients with Down syndrome regularly enrolled in a preventive program. Twenty four Down syndrome patients of both sexes age range of one to 48 years were examined. The prevalence (initial experimental situation) and incidence (final experimental situation) of dental caries were verified using of the initial and final CPO-D, CPO-S, ceo-d and ceo-s indexes of the participants. From 24 individual examined, 10 (42.0%) were free of caries. The prevalence of dental caries showed values of CPO-D= 2.33; CPO-S= 3.60; ceo-d= 1.75 e ceo-s= 2.80; while the incidence of caries showed values of 2.33; 3.80; 1.10 e 1.90, respectively. Down syndrome individuals evaluated in this study presented low level of caries and small incidence of new lesions, emphasizing the importance of the maintenance of these patients at preventive programs.
... (Arnica montana L.), a flor de calêndula (Calendula officinalis L.), as folhas de hamamelis. ... more ... (Arnica montana L.), a flor de calêndula (Calendula officinalis L.), as folhas de hamamelis. (Hamamelis virginiana L.), o fruto de anis-estrelado (Illicium verum Hook.) e as folhas de erva-cidreira (Melissa officinalis L). Neste estudo, foram Page 4. Revista Saúde 14 ...
j o u r n a l o f d e n t i s t r y 4 2 ( 2 0 1 4 ) 1 4 9 5 -1 5 0 1 a b s t r a c t Objectives: ... more j o u r n a l o f d e n t i s t r y 4 2 ( 2 0 1 4 ) 1 4 9 5 -1 5 0 1 a b s t r a c t Objectives: Analyse the effect of varnishes containing xylitol alone or combined with fluoride on the remineralization of artificial enamel caries lesions in vitro.
Glass ionomer cement Chlorhexidine digluconate Cell culture Antibacterial activity Mechanical pro... more Glass ionomer cement Chlorhexidine digluconate Cell culture Antibacterial activity Mechanical properties Indirect pulp therapy a b s t r a c t Objectives: To evaluate: (1) the in vitro antibacterial, cytotoxic and mechanical properties of a resin-modified glass ionomer cement (RMGIC) containing different concentrations of chlorhexidine (CHX) and (2) the in vivo microbiologic action of the best concentration of CHX associated with the RMGIC applied on remaining dentine after indirect pulp treatment (IPT).
a r c h i v e s o f o r a l b i o l o g y 5 7 ( 2 0 1 2 ) 1 3 1 -1 3 8 Glass-ionomer cement Antib... more a r c h i v e s o f o r a l b i o l o g y 5 7 ( 2 0 1 2 ) 1 3 1 -1 3 8 Glass-ionomer cement Antibacterial activity Cell culture Mechanical properties a b s t r a c t Objectives: To characterize the mechanical and biological properties of a resin-modified glass ionomer cement (RMGIC) containing doxycycline hyclate.
Purpose: To evaluate the influence of a 2% chlorhexidine solution (CHX) on the bond strength and ... more Purpose: To evaluate the influence of a 2% chlorhexidine solution (CHX) on the bond strength and nanoleakage of two self-etching adhesive systems on demineralized dentin over a 12-month period.
Objective: To review current models and scientific evidence on the influence of parents' oral hea... more Objective: To review current models and scientific evidence on the influence of parents' oral health behaviors on their children's dental caries. Sources: MEDLINE articles published between 1980 and June, 2012. Original research articles on parents' oral health behavior were reviewed. A total of 218 citations were retrieved, and 13 articles were included in the analysis. The studies were eligible for review if they matched the following inclusion criteria: (1) they evaluated a possible association between dental caries and parents' oral-health-related behaviors, and (2) the study methodology included oral clinical examination. The main search terms were "oral health", "parental attitudes", "parental knowledge", and "dental caries". Summary of the findings: 13 experimental studies contributed data to the synthesis. Original articles, reviews, and chapters in textbooks were also considered. Conclusion: Parents' dental health habits influence their children's oral health. Oral health education programs aimed at preventive actions are needed to provide children not only with adequate oral health, but better quality of life. Special attention should be given to the entire family, concerning their lifestyle and oral health habits.
A series of experiments were conducted to characterize a novel restorative material. We explored ... more A series of experiments were conducted to characterize a novel restorative material. We explored the effect on biological, physical and chemical properties of glass ionomer cement (GIC) adding-the naturally occurring tt-farnesol (900 mM). Two groups were accomplished for all assays: GIC+tt-farnesol and GIC (control). Biological assays: 1) agar diffusion against some cariogenic bacteria; 2) S. mutans biofilm formation and confocal laser scanning microscopy-CLSM. 3) gtfB, gtfC, gtfD, gbpB, vicR, and covR expression; 4) MTT and microscopic morphology. Physical properties assays: 1) roughness; 2) hardness; 3) compressive strength and 4) diametral tensile strength. Chemical assay: Raman spectroscopy. The adding of tt-farnesol to GIC led to larger zones of inhibition (p<0.05), biofilms with a short-term reduction in bacterial viability but similar biomass (p>0.05). Polysaccharides levels increased over time, similarly over groups (p>0.05). Viable and non-viable S. mutans were seen on the specimens' surface by CLSM but their virulence was not modulated by tt-farne-sol. The tt-farnesol increased the HaCaT cell viability without impact on compressive and diametral tensile strength and roughness although the hardness was positively affected (p<0.05). Raman confirmed the presence of tt-farnesol. The incorporation of tt-farnesol into GIC inhibited the growth of cariogenic bacteria but had a little effect on the composition, structure and physiology of the biofilm matrices. Also, the tt-farnesol increased the hardness and the biocompatibility of the GIC, not influencing negatively other physical properties of the restorative material.
In vitro and in vivo evaluations of glass-ionomer cement containing chlorhexidine for Atraumatic ... more In vitro and in vivo evaluations of glass-ionomer cement containing chlorhexidine for Atraumatic Restorative Treatment Objectives: Addition of chlorhexidine has enhanced the antimicrobial effect of glass ionomer cement (GIC) indicated to Atraumatic Restorative Treatment (ART); however, the impact of this mixture on the properties of these materials and on the longevity of restorations must be investigated. The aim of this study was to evaluate the effects of incorporating chlorhexidine (CHX) in the in vitro biological and chemical-mechanical properties of GIC and in vivo clinical/ microbiological follow-up of the ART with GIC containing or not CHX. Material and Methods: For in vitro studies, groups were divided into GIC, GIC with 1.25% CHX, and GIC with 2.5% CHX. Antimicrobial activity of GIC was analyzed A randomized controlled trial was conducted on 36 children that received ART mutans streptococci (MS) counts and the survival rate of restorations was checked after 7 days, 3 months and one year after ART. ANOVA/Tukey or Kruskal-Wallis/ Mann-Whitney tests were performed for in vitro tests and in vivo microbiological analysis. The Kaplan-Meier method and Log rank tests were applied to estimate survival percentages of restorations (p<0.05). Results: Incorporation of 1.25% affecting F release and mechanical characteristics, but 2.5% CHX was cytotoxic. Survival rate of restorations using GIC with 1.25% CHX was similar to GIC. A 1.25% CHX increased the in vitro antimicrobial activity, without changing chemical-mechanical properties of GIC and odontoblast-like cell viability. This combination improved the in vivo short-term microbiological effect without affecting clinical performance of ART restorations. 2017;25(5):541-50 542 J Appl Oral Sci.
Background: Streptococcus mutans orchestrates the development of a biofilm that causes dental car... more Background: Streptococcus mutans orchestrates the development of a biofilm that causes dental caries in the presence of dietary sucrose, and, in the bloodstream, S. mutans can cause systemic infections. The development of a cariogenic biofilm is dependent on the formation of an extracellular matrix rich in exopolysaccharides, which contains extracellular DNA (eDNA) and lipoteichoic acids (LTAs). While the exopolysaccharides are virulence markers, the involvement of genes linked to eDNA and LTAs metabolism in the pathogenicity of S. mutans remains unclear. Objective and Design: In this study, a parental strain S. mutans UA159 and derivative strains carrying single gene deletions were used to investigate the role of eDNA (ΔlytS and ΔlytT), LTA (ΔdltA and ΔdltD), and insoluble exopolysaccharides (ΔgtfB) in virulence in a rodent model of dental caries (rats) and a systemic infection model (Galleria mellonella larvae). Results: Fewer carious lesions were observed on smooth and sulcal surfaces of enamel and dentin of the rats infected with ΔlytS, ΔdltD, and ΔgtfB (vs. the parental strain). Moreover, strains carrying gene deletions prevented the killing of larvae (vs. the parental strain). Conclusions: Altogether, these findings indicate that inactivation of lytST and dltAD impaired S. mutans cariogenicity and virulence in vivo.
This study evaluated gloss and color changes of esthetic restorative materials subjected to diffe... more This study evaluated gloss and color changes of esthetic restorative materials subjected to different acidic beverages. Specimens of resin composites (Z350XT (Z350), IPS Empress Direct (ED), Charisma Diamond (CD)) were prepared and the initial surface gloss and color (ΔE) were measured (n=10). Then, the specimens were immersed in 4 mL of each of the different beverages (cranberry juice; Coca-Cola; coffee or artificial saliva) during 15 min, 3x/day for 14 days and new gloss and color readings were obtained. Color change was evaluated with the ΔE formula and gloss change values were obtained by the formula: (final gloss-initial gloss). Data was submitted to two-way ANOVA followed by Tukey's post hoc test (α=0.05). CD showed the lowest color change among resin composites. The highest ΔE values were obtained after immersion in coffee and cranberry juice. Coffee promoted the highest gloss change (worst gloss retention), followed by cranberry juice, Coca-cola and artificial saliva (p<0.05). The type of beverage significantly influenced the gloss of resin composites. Coca-cola reduced gloss of the three resin composites in a similar manner. Coffee affected the ED gloss more than that of Z350 and CD, while cranberry juice affected Z350 more than ED and CD. Saliva had a more pronounced effect on the gloss retention of CD than ED. The beverages used in this study influenced the optical surface properties of the composites studied.
Objectives: The present study aimed to assess the antibacterial activity against bacteria with ca... more Objectives: The present study aimed to assess the antibacterial activity against bacteria with cariogenic relevance , toxic and genotoxic potential of the plants Anacardium occidentale L. and Anadenanthera macrocarpa (Benth.) Bernam. Design: Using a microdilution technique, the extracts were submitted to minimum inhibitory concentration (MIC) testing against Streptococcus mitis (ATCC 903), Streptococcus mutans (ATCC 25175), Streptococcus oralis (ATCC 10557), Streptococcus salivarius (ATCC 7073), Streptococcus sanguinis (ATCC 15300) and Streptococcus sobrinus (ATCC 27609). The toxicity of the extracts was then verified against eukaryotic cells. Additionally, a micronucleus assay was performed to investigate the potential mutagenic effects of the extracts on rat ery-throcytes. The Student's t-test, Bonferroni test, and one-way ANOVA followed by Tukey's tests were used for statistical analysis, at a significance level of 5%. Results: While the A. occidentale extract was able to inhibit all of the tested strains, with S. mutans and S. mitis being the most susceptible to that extract́sextract́s action, the A. macrocarpa did not show antimicrobial activity. Interestingly, the hemolytic, oxidant and antioxidant activities were slightly observed for either extract, even at high concentrations (1000 mg/mL). The micronucleus assay showed no significant changes in the cells exposed to the extracts. Conclusion: The A. occidentale extract has potential as an antimicrobial agent with low eukaryotic cell toxicity or mutagenic activity. The A. macrocarpa extract, although absent of antibacterial activity might as well be a safe and effective phytotherapeutic alternative.
www.scielo.br/jaos http://dx. Objective: This in vitro study assessed the anti-erosive effect of ... more www.scielo.br/jaoshttp://dx. Objective: This in vitro study assessed the anti-erosive effect of experimental mouthrinses containing TiF 4 and NaF on dentin erosive loss. Material and Methods: Bovine dentin specimens were randomly allocated into the groups (n=15): 1) SnCl 2 /NaF/AmF (Erosion Protection ® /GABA, pH 4.5, positive control); 2) experimental solution with 0.0815% TiF 4 (pH 2.5); 3) 0.105% NaF (pH 4.5); 4) 0.042% NaF+0.049% TiF 4 (pH 4.4); 5) 0.063% NaF+0.036% TiF 4 (pH 4.5); 6) no treatment (negative control). Each specimen was cyclically demineralied (Sprite ero, pH 2.6, 4x90 s/day) and exposed to articial saliva between the erosive challenges for days. The treatment with the uoride solutions was done 2x60 s/day, immediately after the rst and the last erosive challenges of the day. entin erosive loss was measured by prolometry (m). The data were analyed using rusal allis/ Dunn tests (p<0.05). Results: Mouthrinses containing TiF 4 or Sn/F were able to show some protective effect against dentin erosive loss compared to negative control. The best anti-erosive effect was found for experimental solution containing 0.0815% TiF 4 (100% reduction in dentin loss), followed by 0.042% NaF+0.049% TiF 4 (58.3%), SnCl 2 /NaF/AmF (52%) and 0.063% NaF+0.036% TiF 4 (40%). NaF solution (13.3%) did not signicantly differ from control. Conclusion: The daily application of experimental mouthrinse containing TiF 4 and NaF has the ability to reduce dentin erosion, as well as Erosion Protection ® and TiF 4 alone.
Bonding to demineralized dentin of a diseased tooth has shown to be a significant clinical issue.... more Bonding to demineralized dentin of a diseased tooth has shown to be a significant clinical issue. This study evaluated the effect of 0.2% NaF-(NaF), MI Paste™-(CPP-ACP) and the self-assembling peptide 'P 11−4 ′ (Ace-QQRFEWEFEQQ-NH 2) contained in Curodont™ Repair, have on microtensile bond strength-(µTBS) of two different adhesive systems (Adper™ Single Bond-(SB) or Clearfil™ SE Bond (CSE)) and wettability of deminer-alized dentin slices after remineralising agents were applied. The highest µTBS were found for the demineralized dentin-(DD) treated with CPP-ACP; both adhesives systems (p < 0.05) did not significantly difference from P 11-4 treatment associated with SB, and also presented higher values than sound dentin-(SD/SB) (p < 0.01). DD treated with P 11−4 associated with CSE did not differ from DD/CSE (p > 0.05). The NaF treatment associated with CSE recovered the bond strength values of SD/CSE and associated with CSE demonstrated lower µTBS than other groups, although significantly higher than DD (p < 0.05). P 11−4 and CPP-ACP increased significantly the wettability of demineralized dentin (p < 0.05); etching acid improved wettability for all groups (p < 0.05), whilst NaF did not affect the wettability of demineralized dentin (p > 0.05). Morphological analysis of the dentin surface and dentin-resin interface revealed unique features of the applied remineralizing agent. The results indicated that self-assembling peptide P 11−4 associated with SB and CPP-ACP associated with SB or CSE significantly enhanced the bond strength to demineralized dentin (p < 0.05). We conclude that by modifying the dentine surface and restoring conditions found on sound dentin, this can enhance the interfacial bonding.
In vitro and in vivo evaluations of glass-ionomer cement containing chlorhexidine for Atraumatic ... more In vitro and in vivo evaluations of glass-ionomer cement containing chlorhexidine for Atraumatic Restorative Treatment Objectives: Addition of chlorhexidine has enhanced the antimicrobial effect of glass ionomer cement (GIC) indicated to Atraumatic Restorative Treatment (ART); however, the impact of this mixture on the properties of these materials and on the longevity of restorations must be investigated. The aim of this study was to evaluate the effects of incorporating chlorhexidine (CHX) in the in vitro biological and chemical-mechanical properties of GIC and in vivo clinical/ microbiological follow-up of the ART with GIC containing or not CHX. Material and Methods: For in vitro studies, groups were divided into GIC, GIC with 1.25% CHX, and GIC with 2.5% CHX. Antimicrobial activity of GIC was analyzed A randomized controlled trial was conducted on 36 children that received ART mutans streptococci (MS) counts and the survival rate of restorations was checked after 7 days, 3 months and one year after ART. ANOVA/Tukey or Kruskal-Wallis/ Mann-Whitney tests were performed for in vitro tests and in vivo microbiological analysis. The Kaplan-Meier method and Log rank tests were applied to estimate survival percentages of restorations (p<0.05). Results: Incorporation of 1.25% affecting F release and mechanical characteristics, but 2.5% CHX was cytotoxic. Survival rate of restorations using GIC with 1.25% CHX was similar to GIC. A 1.25% CHX increased the in vitro antimicrobial activity, without changing chemical-mechanical properties of GIC and odontoblast-like cell viability. This combination improved the in vivo short-term microbiological effect without affecting clinical performance of ART restorations. 2017;25(5):541-50 542 J Appl Oral Sci.
The aim of this study was to determine the physicochemical characteristics of leaves of Syzygium ... more The aim of this study was to determine the physicochemical characteristics of leaves of Syzygium cumini L. Skeels plant and characterize the extract of this plant by analytical techniques. Pharmacopeial methods of physicochemical analysis were used, including morphological characterization of the particle, thermal analysis, infrared spectroscopy and X-ray diffraction. The plant drug was presented as a coarse powder, within the appropriate Brazilian Pharmacopoeia parameters. The X-ray diffrac-tion, optical microscopy and scanning electron microscopy suggested that the extract particles are amorphous and have irregular shapes, so that clusters of different sizes and morphologies are displayed. Thermal decomposition of the organic components in the sample started in a step that occurred between 151.64 and 209.27 °C with mass loss of 9.08 %, followed by another step with more significant mass loss (28.16 %). The infrared spectrum, in turn, showed many functional groups of compounds present in the lyophilized extract in different absorption bands. The results showed that the analytical techniques allowed us to characterize the physicochemical properties of the plant leaves, which may be useful in the production of new herbal medicines.
2016): Antifungal potential of Sideroxylon obtusifolium and Syzygium cumini and their mode of act... more 2016): Antifungal potential of Sideroxylon obtusifolium and Syzygium cumini and their mode of action against Candida albicans, Pharmaceutical Biology
4 Doutora em Patologia Bucal pela Faculdade de Odontologia de Bauru da USP e professora do Depart... more 4 Doutora em Patologia Bucal pela Faculdade de Odontologia de Bauru da USP e professora do Departamento de Odontologia da USC. Bauru, SP, Brasil 5 Graduado em Odontologia e aluno de especialização em Bucomaxilo Facial pela Faculdade de Odontologia de Bauru da USC. Bauru, SP, Brasil Resumo Objetivo: Apresentar as características clínicas, trata-mento cirúrgico e achado histológico de um caso de lipoi-doproteinose. Descrição do caso: Criança do sexo masculino, cinco anos de idade, branco, que procurou atendimento odontológico na Uni-versidade. A mãe da criança relatou presença de intensa halitose e dificuldade na alimentação e higienização bucal, decorrentes de crescimento gengival generalizado nos arcos dentários superior e inferior. No exame clínico, verificaram-se comprometimento funcional e estético generalizado (rouquidão, artralgia bilateral no joelho e tornozelo, lesões tumorais nas orelhas, entre outros), além de extensa hiperplasia gengival em ambos os arcos den-tários. Optou...
ABSTRACT The objective of this work was to verify the incidence of dental caries by means of the ... more ABSTRACT The objective of this work was to verify the incidence of dental caries by means of the CPO-D, CPO-S, ceo-d and ceo-s indexes in patients with Down syndrome regularly enrolled in a preventive program. Twenty four Down syndrome patients of both sexes age range of one to 48 years were examined. The prevalence (initial experimental situation) and incidence (final experimental situation) of dental caries were verified using of the initial and final CPO-D, CPO-S, ceo-d and ceo-s indexes of the participants. From 24 individual examined, 10 (42.0%) were free of caries. The prevalence of dental caries showed values of CPO-D= 2.33; CPO-S= 3.60; ceo-d= 1.75 e ceo-s= 2.80; while the incidence of caries showed values of 2.33; 3.80; 1.10 e 1.90, respectively. Down syndrome individuals evaluated in this study presented low level of caries and small incidence of new lesions, emphasizing the importance of the maintenance of these patients at preventive programs.
ABSTRACT The objective of this work was to verify the incidence of dental caries by means of the ... more ABSTRACT The objective of this work was to verify the incidence of dental caries by means of the CPO-D, CPO-S, ceo-d and ceo-s indexes in patients with Down syndrome regularly enrolled in a preventive program. Twenty four Down syndrome patients of both sexes age range of one to 48 years were examined. The prevalence (initial experimental situation) and incidence (final experimental situation) of dental caries were verified using of the initial and final CPO-D, CPO-S, ceo-d and ceo-s indexes of the participants. From 24 individual examined, 10 (42.0%) were free of caries. The prevalence of dental caries showed values of CPO-D= 2.33; CPO-S= 3.60; ceo-d= 1.75 e ceo-s= 2.80; while the incidence of caries showed values of 2.33; 3.80; 1.10 e 1.90, respectively. Down syndrome individuals evaluated in this study presented low level of caries and small incidence of new lesions, emphasizing the importance of the maintenance of these patients at preventive programs.
... (Arnica montana L.), a flor de calêndula (Calendula officinalis L.), as folhas de hamamelis. ... more ... (Arnica montana L.), a flor de calêndula (Calendula officinalis L.), as folhas de hamamelis. (Hamamelis virginiana L.), o fruto de anis-estrelado (Illicium verum Hook.) e as folhas de erva-cidreira (Melissa officinalis L). Neste estudo, foram Page 4. Revista Saúde 14 ...
j o u r n a l o f d e n t i s t r y 4 2 ( 2 0 1 4 ) 1 4 9 5 -1 5 0 1 a b s t r a c t Objectives: ... more j o u r n a l o f d e n t i s t r y 4 2 ( 2 0 1 4 ) 1 4 9 5 -1 5 0 1 a b s t r a c t Objectives: Analyse the effect of varnishes containing xylitol alone or combined with fluoride on the remineralization of artificial enamel caries lesions in vitro.
Glass ionomer cement Chlorhexidine digluconate Cell culture Antibacterial activity Mechanical pro... more Glass ionomer cement Chlorhexidine digluconate Cell culture Antibacterial activity Mechanical properties Indirect pulp therapy a b s t r a c t Objectives: To evaluate: (1) the in vitro antibacterial, cytotoxic and mechanical properties of a resin-modified glass ionomer cement (RMGIC) containing different concentrations of chlorhexidine (CHX) and (2) the in vivo microbiologic action of the best concentration of CHX associated with the RMGIC applied on remaining dentine after indirect pulp treatment (IPT).
a r c h i v e s o f o r a l b i o l o g y 5 7 ( 2 0 1 2 ) 1 3 1 -1 3 8 Glass-ionomer cement Antib... more a r c h i v e s o f o r a l b i o l o g y 5 7 ( 2 0 1 2 ) 1 3 1 -1 3 8 Glass-ionomer cement Antibacterial activity Cell culture Mechanical properties a b s t r a c t Objectives: To characterize the mechanical and biological properties of a resin-modified glass ionomer cement (RMGIC) containing doxycycline hyclate.
Purpose: To evaluate the influence of a 2% chlorhexidine solution (CHX) on the bond strength and ... more Purpose: To evaluate the influence of a 2% chlorhexidine solution (CHX) on the bond strength and nanoleakage of two self-etching adhesive systems on demineralized dentin over a 12-month period.
Objective: To review current models and scientific evidence on the influence of parents' oral hea... more Objective: To review current models and scientific evidence on the influence of parents' oral health behaviors on their children's dental caries. Sources: MEDLINE articles published between 1980 and June, 2012. Original research articles on parents' oral health behavior were reviewed. A total of 218 citations were retrieved, and 13 articles were included in the analysis. The studies were eligible for review if they matched the following inclusion criteria: (1) they evaluated a possible association between dental caries and parents' oral-health-related behaviors, and (2) the study methodology included oral clinical examination. The main search terms were "oral health", "parental attitudes", "parental knowledge", and "dental caries". Summary of the findings: 13 experimental studies contributed data to the synthesis. Original articles, reviews, and chapters in textbooks were also considered. Conclusion: Parents' dental health habits influence their children's oral health. Oral health education programs aimed at preventive actions are needed to provide children not only with adequate oral health, but better quality of life. Special attention should be given to the entire family, concerning their lifestyle and oral health habits.
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