Papers by MONICA MARILENA MIAZZI
Horticulturae, Apr 22, 2024
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Agriculture, Mar 18, 2024
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Frontiers in plant science, Apr 5, 2024
The growing demand for sustainable and environmentally friendly viticulture is leading to a multi... more The growing demand for sustainable and environmentally friendly viticulture is leading to a multiplication of breeding programs aimed at obtaining vines that are resistant to powdery mildew (PM) and downy mildew (DM), the two most damaging vine diseases. In Puglia, the most important Italian region for the production of table grapes, an extensive crossing program was launched in 2015 with 113 crosses, including elite table varieties, seedless varieties, and resistant varieties. The main seedling production parameters were measured for each cross. In particular, berries harvested as well as the number of seeds and seedlings obtained were considered. Approximately 103,119 seedlings were obtained and subjected to marker-assisted selection for seedlessness using the marker VvAGL11 and for resistance to PM and DM with appropriate markers. Approximately one third (32,638) of the progenies were selected as putative seedless and seventeen thousand five hundred-nine (17,509) were transferred to the field for phenotypic evaluation, including 527 seedless individuals putatively resistant, of which 208 confirmed to be resistant to DM, 22 resistant to PM, and 20 individuals that combined resistance and seedlessness traits. The work discusses the effects of parental combinations and other variables in obtaining surviving progeny and pyramiding genes in table grapes and provides useful information for selecting genotypes and increasing the efficiency of breeding programs for seedless disease-resistant grapes.
Genomics, Sep 1, 2021
Studying and understanding the genetic basis of polyphenol oxidases (PPO)-related traits plays a ... more Studying and understanding the genetic basis of polyphenol oxidases (PPO)-related traits plays a crucial role in genetic improvement of crops. A tetraploid wheat collection (T. turgidum ssp., TWC) was analyzed using the 90 K wheat SNP iSelect assay and phenotyped for PPO activity. A total of 21,347 polymorphic SNPs were used to perform genome-wide association analysis (GWA) in TWC and durum wheat sub-groups, detecting 23 and 85 marker-trait associations (MTA). In addition, candidate genes responsible for PPO activity were predicted. Based on the 23 MTA detected in TWC, two haplotypes associated with low and high PPO activity were identified. Four SNPs were developed and validated providing one reliable marker (IWB75732) for marker assisted selection. The 23 MTAs were used to evaluate the genetic divergence (FST > 0.25) between the T. turgidum sub-species, providing new information important for understanding the domestication process of Triticum turgidum ssp. and in particular of ssp. carthlicum.
In Vitro Cellular & Developmental Biology – Plant, May 5, 2010
Journal of Phytopathology, Apr 27, 2011
Powdery mildews, caused by Golovinomyces cichoracearum and Podosphaera xanthii, are the most comm... more Powdery mildews, caused by Golovinomyces cichoracearum and Podosphaera xanthii, are the most common and severe diseases of cucurbits in the Mediterranean basin. In southern Italy, only P. xanthii is apparently present. However, information on its distribution and population biology is scanty, although it would be crucial for a more rational disease control. Studies addressing physiological races, mating types and RAPD analysis were carried out on 82 isolates of P. xanthii sampled in 34 cucurbit fields from Apulia (southern Italy). A set of eight differential melon genotypes were used to discriminate physiological races of the fungus. In particular, 13% of the tested isolates belonged to physiological race 2 FR, 30% to race 5, 25% to race 1, 10% to race 3, 5% to race 4, 1% to race 0 and 16% to undetermined races, whereas only one of the two mating types (MAT1-2) of the fungus was detected, and RAPD analysis showed a quite broad variation within fungal isolates.
Journal of Plant Pathology, 2011
Genes, Feb 24, 2020
Cultivation of faba bean (Vicia faba L.) in Tunisia is largely based on improved varieties of the... more Cultivation of faba bean (Vicia faba L.) in Tunisia is largely based on improved varieties of the crop. However, a few farmers continue to produce local cultivars or landraces. The National Gene Bank of Tunisia (NGBT) recently launched a collection project for faba bean landraces, with special focus on the regions of the North West, traditionally devoted to cultivating grain legumes, and where around 80% of the total national faba bean cultivation area is located. The seed phenotypic features of the collected samples were studied, and the genetic diversity and population structure analyzed using simple sequence repeat markers. The genetic constitution of the present samples was compared to that of faba bean samples collected by teams of the International Center for Agricultural Research in the Dry Areas (ICARDA) in the 1970s in the same region, and stored at the ICARDA gene bank. The results of the diversity analysis demonstrate that the recently collected samples and those stored at ICARDA largely overlap, thus demonstrating that over the past 50 years, little genetic change has occurred to the local faba bean populations examined. These findings suggest that farmers serendipitously applied international best practices for in situ conservation of agricultural crops.
Journal of Phytopathology, Mar 8, 2018
Wilma Sabetta, Cinzia Montemurro and Monica Marilena Miazzi, (2019). Variation of Phomopsis vitic... more Wilma Sabetta, Cinzia Montemurro and Monica Marilena Miazzi, (2019). Variation of Phomopsis viticola isolates in the interaction with grapevine germplasm. Journal of Phytopathology, 167, 312. https://doi.org/10.1111/jph.12699The above article from Journal of Phytophathology, published as an Early View article online on 08 March 2018 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors, Wilma Sabetta, Cinzia Montemurro and Monica Marilena Miazzi, the journal Editor in Chief, Andreas von Tiedemann, and John Wiley and Sons Ltd. The retraction has been made as a consequence of a disagreement between the authors as to the conclusions of the study.
HAL (Le Centre pour la Communication Scientifique Directe), Jun 23, 2013
KN.2.1 An overview of Botrytis cinerea populations causing gray mold in table grapes, kiwifruits ... more KN.2.1 An overview of Botrytis cinerea populations causing gray mold in table grapes, kiwifruits and blueberries in Chile.
Zenodo (CERN European Organization for Nuclear Research), Nov 22, 2021
The olive tree is one of the most important economic, cultural, and environmental resources for t... more The olive tree is one of the most important economic, cultural, and environmental resources for the Mediterranean basin. The increasing attention to the continuous loss of plant genetic diversity due to social, economic and climatic changes, has favoured a renewed interest in strategies aimed at the recovery and conservation of these genetic resources. In the frame of a project for the valorization of the olive Apulian biodiversity (Re.Ger.O.P. project), 177 minor genotypes were recovered and submitted to morphological, molecular, technological and phytosanitary status analysis in comparison with reference cultivars, then they were propagated and transferred in an ex situ field. In the meantime, nine minor Italian olive genotypes cultivated in three Italian regions has been molecularly fingerprinted with 12 nuclear microsatellites (SSRs), that were able to unequivocally identify all genotypes. Moreover, some of the principal phenolic compounds were determined and quantified in monovarietal oils and the expression levels of related genes were also investigated at different fruit developmental stages. Genotypes differed to the greatest extent in the content of oleacein (3,4-DHPEA-EDA) and total phenols. Thereby, minor local genotypes, characterized by stable production and resilience in a low-input agro-system, can provide a remarkable contribution to the improvement of the Italian olive production chain and can become very profitable from a socio-economic point of view.
HAL (Le Centre pour la Communication Scientifique Directe), Mar 14, 2017
Agronomy, Apr 14, 2020
The investigation on the genetic diversity of grapevine germplasm is crucial for a more efficient... more The investigation on the genetic diversity of grapevine germplasm is crucial for a more efficient use of grapevine genetic resources in light of changing environmental conditions. Here, we used simple sequence repeats (SSRs) coupled with single nucleotide polymorphism (SNP) markers to disclose grapevine genetic diversity of a collection of Apulian minor/neglected genotypes. Their relationships with national or international cultivars were also examined. Genetic diversity was investigated using 10 SSR markers and 1,178 SNPs generated by genotyping by sequencing (GBS). Based on the SSR data, the 128 genotypes were classified into six main genetic clusters. Twenty-four putative cases of synonymy and 2 of misnamings were detected. Ten "unknown" autochthonous genotypes did not show high similarity to Apulian, national, or international varieties. We took advantage of available GBS-derived SNP data points for only forty genotypes to better investigate the genetic distance among them, identify private SNP alleles, and divergent loci putatively under selection. Based on SNP alleles, two interesting gene pools of minor/neglected Apulian samples were identified. Genetic divergence was investigated by F ST and allowed the detection of loci capable of differentiating the gene pools. Overall, this work emphasizes the need for recovering the untapped genetic variability that characterizes minor/neglected grapevine Apulian genotypes and the requirement to preserve and use more efficiently grapevine genetic resources in breeding programs.
Frontiers in Plant Science
Sexual reproduction has contributed to a significant degree of variability in cultivated grapevin... more Sexual reproduction has contributed to a significant degree of variability in cultivated grapevine populations. However, the additional influence of spontaneous somatic mutations has played a pivotal role in shaping the diverse landscape of grapevine agrobiodiversity. These naturally occurring selections, termed 'clones,' represent a vast reservoir of potentially valuable traits and alleles that hold promise for enhancing grape quality and bolstering plant resilience against environmental and biotic challenges. Despite their potential, many of these clones remain largely untapped.In light of this context, this study aims to delve into the population structure, genetic diversity, and distinctive genetic loci within a collection of 138 clones derived from six Campanian and Apulian grapevine varieties, known for their desirable attributes in viticulture and winemaking. Employing two reduced representation sequencing methods, we extracted Single-Nucleotide Polymorphism (SNP) mar...
Plants
Genetic structure and distinctive features of landraces, such as adaptability to local agro-ecosy... more Genetic structure and distinctive features of landraces, such as adaptability to local agro-ecosystems and specific qualitative profiles, can be substantially altered by the massive introduction of allochthonous germplasm. The landrace known as “Cipolla rossa di Acquaviva” (Acquaviva red onion, further referred to as ARO) is traditionally cultivated and propagated in a small area of the Apulia region (southern Italy). However, the recent rise of its market value and cultivation area is possibly causing genetic contamination with foreign propagating material. In this work, genotyping-by-sequencing (GBS) was used to characterize genetic variation of seven onion populations commercialized as ARO, as well as one population of the landrace “Montoro” (M), which is phenotypically similar, but originates from another cultivation area and displays different qualitative features. A panel of 5,011 SNP markers was used to perform parametric and non-parametric genetic structure analyses, which s...
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Papers by MONICA MARILENA MIAZZI