In this work, an evaluation of the biological activities of Globularia alypum L. extracts and the... more In this work, an evaluation of the biological activities of Globularia alypum L. extracts and their global chemical composition was realized. Extracts from G. alypum were obtained by two extraction methods. The composition of polyphenols (8.5-139.95 g gallic acid equivalent/Kg of dry mass), tannins (1.39-18.65 g catechin equivalent/Kg of dry mass), anthocyanins (8.17-70.69 mg cyanidin equivalent/Kg of dry mass) and flavonoids (0.31-19.28 g quercetin equivalent/Kg of dry mass) was evaluated. The samples were subjected to a screening for their antioxidant activities using the DPPH and ABTS assays. For the first time, the anti-tuberculosis activity (H 37 Rv) for G. alypum was tested against Mycobacterium tuberculosis. The strongest antioxidant activity was obtained for the methanol extract (IC 50 = 15.58 ± 0.168 mg/L) and the best anti-tuberculosis activity was obtained for the petroleum ether extract (IC 50 = 77 mg/L). We have found a positive correlation between the total phenolics content and the antioxidant activity R 2 = 0.88 (DPPH ) and R 2 = 0.97 (ABTS ). We have found also a positive correlation OPEN ACCESS between the flavonoid content and the antioxidant activity R 2 = 0.91 (DPPH ) and R 2 = 0.91 (ABTS ).
ABSTRACT Extracts (petroleum ether, dichloromethane, acetone, methanol/water (3/1) and water) fro... more ABSTRACT Extracts (petroleum ether, dichloromethane, acetone, methanol/water (3/1) and water) from Cynodon dactylon L. were obtained by sequential method. The chemical composition of phenolics (6.9–57.41 g gallic acid equivalents/kg of dry mass), tannins (2.49–12.32 g catechin equivalent/kg of dry mass), anthocyanins (0–5.98 g cyanidin equivalent/kg of dry mass) and flavonoids (0–5.02 g quercetin equivalent/kg of dry mass) was evaluated. The extracts were evaluated for their antioxidant (DPPH and ABTS assays), antimalarial (Plasmodium falciparum) and anticancer (MCF-7) activities; the strongest activities were obtained by the water extract (IC50 = 57.21 ± 1.47 mg/L), the acetonic extract (IC50 = 38 mg/L) and the petroleum ether extract (IC50 = 39 mg/L), respectively. The results indicate a good correlations between anthocyanins quantity and the antimalarial activity (R2 = 0.79) and also to anticancer activity (R2 = 0.78). The LC–MS analysis of the extracts having the good activities anticancer and antimalarial, has revealed the presence of seven anthocyanins (delphinidin-3-O-acetylglucoside, petunidin-3-O-caffeoylglucoside-5-O-glucoside, petunidin-3-O-coumarylglucoside-5-O-glucoside, malvidin-3-O-monoglucoside, delphinidin-3-O-acetylglucoside-pyruvic acid, petunidin-3-O-acetylglucoside-5-O-glucoside and cyanidin-3,5-O-diglucoside), identified for the first time in this plant.
In this study, biological activities of methanolic extracts from Artemisia herba-alba, Ruta chalp... more In this study, biological activities of methanolic extracts from Artemisia herba-alba, Ruta chalpensis L. and Peganum harmala L. plants, collected in Centre of Tunisia, were investigated. Results showed an important phenolic composition of Artemisia herba-alba (123.95 ± 4.3 g GAE/kg of dry mass). The extract of this plant showed, using different antioxidant assays (DPPH, ABTS and AAPH/linoleic acid methods) and an IFN-c/ LPS induced RAW 264.7 murine macrophages' assay, the highest antioxidant (IC50 (DPPH assay) 20.64 ± 0.84 mg/L) and anti-inflammatory (72% inhibition at 150 mg/L) activities, respectively. Excepting Peganum harmala L. extract, the two other extracts showed a high anticancer activity against several cell lines (human bladder carcinoma RT112, human laryngeal carcinoma Hep2 and human myelogenous leukemia K562), for A. herba-laba IC50 = 81.59 ± 4.4, 59.05 ± 3.66 and 90.96 mg/L respectively, but not on normal peripheral blood mononuclear cells. All these biological activities are well correlated with the phenolic contents of these extracts. These findings demonstrate the remarkable potential of these plants as valuable source of antioxidants with exhibit original and interesting anti-inflammatory and anticancer capacities.
Abstract-The Globularia alypum methanolic extract (GAME) was evaluated for the anticholinestera... more Abstract-The Globularia alypum methanolic extract (GAME) was evaluated for the anticholinesterase, anti 5lipoxygenase, NO production inhibitory activities and the transcriptional regulation pathway. Interestingly, GAME showed an important anti-inflammatory activity in a dose dependant manner, and inhibited nitric oxide (NO) production via transcriptional regulation of iNOS gene by (66%) at 150mg/L in IFN-γ/LPS stimulated RAW 264.7 macrophages. In addition, both Globularia alypum showed a 5-Lipoxygenase inhibitory activity with IC 50 value of 79±0.8mg/L. Acetyl-cholinesterase inhibition was assessed by modification of the Ellman's method. Globularia alypum exhibited a strong activity against cholinesterase with IC 50 value 9.33±0.47mg/L. The data suggest that Globularia alypum extract could be used as a natural inhibitor of oxidation and alzheimer disease, and since GAME induced a potent anti-inflammatory suggest its potent use for the treatment of inflammatory diseases.
In this work, an evaluation of the biological activities of Globularia alypum L. extracts and the... more In this work, an evaluation of the biological activities of Globularia alypum L. extracts and their global chemical composition was realized. Extracts from G. alypum were obtained by two extraction methods. The composition of polyphenols (8.5-139.95 g gallic acid equivalent/Kg of dry mass), tannins (1.39-18.65 g catechin equivalent/Kg of dry mass), anthocyanins (8.17-70.69 mg cyanidin equivalent/Kg of dry mass) and flavonoids (0.31-19.28 g quercetin equivalent/Kg of dry mass) was evaluated. The samples were subjected to a screening for their antioxidant activities using the DPPH and ABTS assays. For the first time, the anti-tuberculosis activity (H 37 Rv) for G. alypum was tested against Mycobacterium tuberculosis. The strongest antioxidant activity was obtained for the methanol extract (IC 50 = 15.58 ± 0.168 mg/L) and the best anti-tuberculosis activity was obtained for the petroleum ether extract (IC 50 = 77 mg/L). We have found a positive correlation between the total phenolics content and the antioxidant activity R 2 = 0.88 (DPPH ) and R 2 = 0.97 (ABTS ). We have found also a positive correlation OPEN ACCESS between the flavonoid content and the antioxidant activity R 2 = 0.91 (DPPH ) and R 2 = 0.91 (ABTS ).
ABSTRACT Extracts (petroleum ether, dichloromethane, acetone, methanol/water (3/1) and water) fro... more ABSTRACT Extracts (petroleum ether, dichloromethane, acetone, methanol/water (3/1) and water) from Cynodon dactylon L. were obtained by sequential method. The chemical composition of phenolics (6.9–57.41 g gallic acid equivalents/kg of dry mass), tannins (2.49–12.32 g catechin equivalent/kg of dry mass), anthocyanins (0–5.98 g cyanidin equivalent/kg of dry mass) and flavonoids (0–5.02 g quercetin equivalent/kg of dry mass) was evaluated. The extracts were evaluated for their antioxidant (DPPH and ABTS assays), antimalarial (Plasmodium falciparum) and anticancer (MCF-7) activities; the strongest activities were obtained by the water extract (IC50 = 57.21 ± 1.47 mg/L), the acetonic extract (IC50 = 38 mg/L) and the petroleum ether extract (IC50 = 39 mg/L), respectively. The results indicate a good correlations between anthocyanins quantity and the antimalarial activity (R2 = 0.79) and also to anticancer activity (R2 = 0.78). The LC–MS analysis of the extracts having the good activities anticancer and antimalarial, has revealed the presence of seven anthocyanins (delphinidin-3-O-acetylglucoside, petunidin-3-O-caffeoylglucoside-5-O-glucoside, petunidin-3-O-coumarylglucoside-5-O-glucoside, malvidin-3-O-monoglucoside, delphinidin-3-O-acetylglucoside-pyruvic acid, petunidin-3-O-acetylglucoside-5-O-glucoside and cyanidin-3,5-O-diglucoside), identified for the first time in this plant.
In this study, biological activities of methanolic extracts from Artemisia herba-alba, Ruta chalp... more In this study, biological activities of methanolic extracts from Artemisia herba-alba, Ruta chalpensis L. and Peganum harmala L. plants, collected in Centre of Tunisia, were investigated. Results showed an important phenolic composition of Artemisia herba-alba (123.95 ± 4.3 g GAE/kg of dry mass). The extract of this plant showed, using different antioxidant assays (DPPH, ABTS and AAPH/linoleic acid methods) and an IFN-c/ LPS induced RAW 264.7 murine macrophages' assay, the highest antioxidant (IC50 (DPPH assay) 20.64 ± 0.84 mg/L) and anti-inflammatory (72% inhibition at 150 mg/L) activities, respectively. Excepting Peganum harmala L. extract, the two other extracts showed a high anticancer activity against several cell lines (human bladder carcinoma RT112, human laryngeal carcinoma Hep2 and human myelogenous leukemia K562), for A. herba-laba IC50 = 81.59 ± 4.4, 59.05 ± 3.66 and 90.96 mg/L respectively, but not on normal peripheral blood mononuclear cells. All these biological activities are well correlated with the phenolic contents of these extracts. These findings demonstrate the remarkable potential of these plants as valuable source of antioxidants with exhibit original and interesting anti-inflammatory and anticancer capacities.
Abstract-The Globularia alypum methanolic extract (GAME) was evaluated for the anticholinestera... more Abstract-The Globularia alypum methanolic extract (GAME) was evaluated for the anticholinesterase, anti 5lipoxygenase, NO production inhibitory activities and the transcriptional regulation pathway. Interestingly, GAME showed an important anti-inflammatory activity in a dose dependant manner, and inhibited nitric oxide (NO) production via transcriptional regulation of iNOS gene by (66%) at 150mg/L in IFN-γ/LPS stimulated RAW 264.7 macrophages. In addition, both Globularia alypum showed a 5-Lipoxygenase inhibitory activity with IC 50 value of 79±0.8mg/L. Acetyl-cholinesterase inhibition was assessed by modification of the Ellman's method. Globularia alypum exhibited a strong activity against cholinesterase with IC 50 value 9.33±0.47mg/L. The data suggest that Globularia alypum extract could be used as a natural inhibitor of oxidation and alzheimer disease, and since GAME induced a potent anti-inflammatory suggest its potent use for the treatment of inflammatory diseases.
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