Integrative and conjugative elements (ICEs) are self-transferable mobile genetic elements that pl... more Integrative and conjugative elements (ICEs) are self-transferable mobile genetic elements that play a significant role in disseminating antimicrobial resistance between bacteria via horizontal gene transfer (HGT). A recently identified ICE in a clinical isolate of Histophilus somni (ICEHs02) is 72,914 base-pairs in length and harbours seven predicted antimicrobial resistance genes (ARG) conferring resistance to tetracycline (tetR-tet(H)), florfenicol (floR), sulfonamide (Sul2), aminoglycoside (APH(3'')-Ib), (APH(6)-Id), (APH(3')-Ia), and copper (mco). This study investigated ICEHs02 host range, assessed effects of antimicrobial stressors on transfer frequency, and examined effects of ICEHs02 acquisition on hosts. Conjugation assays examined transfer frequency of ICEHs02 to Histophilus somni and Pasteurella multocida strains. PCR assays confirmed the presence of a circular intermediate, ICE-associated core genes, and cargo genes in recipient strains. Susceptibility testin...
Integrative and conjugative elements (ICEs) are autonomous-transferred mobile genetic elements th... more Integrative and conjugative elements (ICEs) are autonomous-transferred mobile genetic elements that play a significant role in disseminating antimicrobials between bacteria via horizontal gene transfer (HGT). One recently identified ICE in a clinical isolate of H. somni named ICEHs02 is 72,914 base pairs in length and harbours seven AMR genes which confer resistance to tetracycline (tetR-tet(H)), florfenicol (floR), sulfonamide (sul2), aminoglycoside (APH(3'')-Ib), (APH(6)-Id), and (APH(3')-Ia), and copper (mco). This study investigated the host range of ICEHs02, assessed the effect of antimicrobial stressors on transfer frequency of ICEHs02, and examined effects of ICE acquisition on the host. In vitro conjugation assays were conducted to examine the frequency of transfer of ICEHs02 into other bacteria. PCR and sequence analysis confirmed the presence of ICEHs02 and its circular intermediate in recipient strains. The susceptibility of ICEHs02-carrying recipients was con...
Integrative and conjugative elements (ICEs) are self-transferable mobile genetic elements that pl... more Integrative and conjugative elements (ICEs) are self-transferable mobile genetic elements that play a significant role in disseminating antimicrobial resistance between bacteria via horizontal gene transfer (HGT). A recently identified ICE in a clinical isolate of Histophilus somni (ICEHs02) is 72,914 base-pairs in length and harbours seven predicted antimicrobial resistance genes (ARG) conferring resistance to tetracycline (tetR-tet(H)), florfenicol (floR), sulfonamide (Sul2), aminoglycoside (APH(3'')-Ib), (APH(6)-Id), (APH(3')-Ia), and copper (mco). This study investigated ICEHs02 host range, assessed effects of antimicrobial stressors on transfer frequency, and examined effects of ICEHs02 acquisition on hosts. Conjugation assays examined transfer frequency of ICEHs02 to Histophilus somni and Pasteurella multocida strains. PCR assays confirmed the presence of a circular intermediate, ICE-associated core genes, and cargo genes in recipient strains. Susceptibility testin...
Integrative and conjugative elements (ICEs) are autonomous-transferred mobile genetic elements th... more Integrative and conjugative elements (ICEs) are autonomous-transferred mobile genetic elements that play a significant role in disseminating antimicrobials between bacteria via horizontal gene transfer (HGT). One recently identified ICE in a clinical isolate of H. somni named ICEHs02 is 72,914 base pairs in length and harbours seven AMR genes which confer resistance to tetracycline (tetR-tet(H)), florfenicol (floR), sulfonamide (sul2), aminoglycoside (APH(3'')-Ib), (APH(6)-Id), and (APH(3')-Ia), and copper (mco). This study investigated the host range of ICEHs02, assessed the effect of antimicrobial stressors on transfer frequency of ICEHs02, and examined effects of ICE acquisition on the host. In vitro conjugation assays were conducted to examine the frequency of transfer of ICEHs02 into other bacteria. PCR and sequence analysis confirmed the presence of ICEHs02 and its circular intermediate in recipient strains. The susceptibility of ICEHs02-carrying recipients was con...
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