Papers by Kaat Verplanken
Surgical castration of male pigs will soon be abandoned, turning a major advantage of this practi... more Surgical castration of male pigs will soon be abandoned, turning a major advantage of this practice, the elimination of boar taint, into the biggest challenge for pig industry when raising intact male pigs becomes common practice. To map the (economical) consequences in relation to boar-taint consumer acceptance, as well as offer a processing strategy for tainted carcasses to stockholders, the current study investigated not only back fat boar taint levels, but additionally generated information on the levels of boar taint compounds recovered after the production of commercially relevant meat products using UHPLC-HRMS laboratory analysis. Our results demonstrate that levels of androstenone, skatole and indole in back fat and meat products tend to correlate strongly, particularly in fatty meat products (generally r > 0.80). Concentration values in the edible (lean) meat fraction were significantly lower compared to back fat and fat sampled from fresh or processed meat (p < 0.05).
Boar taint is an off-odour occurring while heating meat or fat from boars. A method detecting the... more Boar taint is an off-odour occurring while heating meat or fat from boars. A method detecting the three compounds (androstenone, skatole and indole) simultaneously in blood would offer substantial advan- tages since it would allow monitoring the impact of rearing strategies.
Therefore, a UHPLC-HR-Orbitrap-MS analysis method is optimized and validated for the quantification of these compounds in plasma or serum. Sample pre-treatment involved an extraction with diethylether followed by a centrifugal filtration (30 kDa).
Limits of detection and quantification varied between 0.5 and 1 lg L1 and 2 and 3 lg L1 for the three compounds, respectively. Besides, an excellent repeatability (RSD < 7.6%), within-laboratory repro- ducibility (RSD < 10.5%), recovery (87–97%) and linearity (R2 > 0.99) were recorded.
Correlations between serum/plasma and fat levels of the boar taint compounds were positive for ska- tole (rserum = 0.39 and rplasma = 0.84) and androstenone (rserum = 0.73–0.78 and rplasma = 0.32–0.80).
Boar taint is an off-odour that entails negative consumer reactions. In this study two extraction... more Boar taint is an off-odour that entails negative consumer reactions. In this study two extraction and UHPLC-HRMS analysis methods, valuable for evaluation of consumer acceptance towards boar meat, were developed for quantification of indole, skatole, and androstenone in different meat products. Sample pretreatment consisted of extraction with methanol and a homogenising step (cooked ham, minced meat, tenderloin, bacon, cutlets, blade loin, uncooked ham) or a melting step (salami sausage and liver paste). Both methods were validated according to CD 2002/657/EC and ISO 17025 guidelines. Good performance characteristics were obtained. Good linearity (R 2 P 0.99) and no lack of fit was observed (95% confidence interval; F-test, p > 0.05). Also good recovery (89–110%) and satisfactory precision: repeatability (RSD 6 14.9%) and within-laboratory reproducibility (RSD 6 17.2%) were obtained. Analysis of cooked ham and salami sausage samples proved the applicability of both methods for routine analysis.
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Papers by Kaat Verplanken
Therefore, a UHPLC-HR-Orbitrap-MS analysis method is optimized and validated for the quantification of these compounds in plasma or serum. Sample pre-treatment involved an extraction with diethylether followed by a centrifugal filtration (30 kDa).
Limits of detection and quantification varied between 0.5 and 1 lg L1 and 2 and 3 lg L1 for the three compounds, respectively. Besides, an excellent repeatability (RSD < 7.6%), within-laboratory repro- ducibility (RSD < 10.5%), recovery (87–97%) and linearity (R2 > 0.99) were recorded.
Correlations between serum/plasma and fat levels of the boar taint compounds were positive for ska- tole (rserum = 0.39 and rplasma = 0.84) and androstenone (rserum = 0.73–0.78 and rplasma = 0.32–0.80).
Therefore, a UHPLC-HR-Orbitrap-MS analysis method is optimized and validated for the quantification of these compounds in plasma or serum. Sample pre-treatment involved an extraction with diethylether followed by a centrifugal filtration (30 kDa).
Limits of detection and quantification varied between 0.5 and 1 lg L1 and 2 and 3 lg L1 for the three compounds, respectively. Besides, an excellent repeatability (RSD < 7.6%), within-laboratory repro- ducibility (RSD < 10.5%), recovery (87–97%) and linearity (R2 > 0.99) were recorded.
Correlations between serum/plasma and fat levels of the boar taint compounds were positive for ska- tole (rserum = 0.39 and rplasma = 0.84) and androstenone (rserum = 0.73–0.78 and rplasma = 0.32–0.80).