Validation of Responsive Genes to Drought Stress in Two Contrasting Cultivars of Sugarcane
Sugarcane is a major crop worldwide, raw material for sugar and ethanol production. Drought is on... more Sugarcane is a major crop worldwide, raw material for sugar and ethanol production. Drought is on of the most limiting factor that affects sugarcane productivity. In order to understand the mechanisms of drought response, field and greenhouse essays were conducted with two drought-contrasting sugarcane genotypes (IACSP94-2094/tolerant and IACSP97-7065/sensitive). A microarray analysis, conducted with 14,522 genes using material grown on field, and a global gene expression analysis performed by RNAseq with plants grown in greenhouse have both identified several genes that are up/down-regulated under drought stress. Among these, nine genes (presented in both assays) were selected to be validated by qPCR using leaves. Four genes (Water Stress, Hormone Biosynthesis, Unknown1 and Unknown2) showed higher levels of transcripts in IACSP97-7065 in greenhouse, while levels in IACSP94-2094 were higher on field. Dehydrin and Cell Cycle genes showed the highest expression levels in sensitive gen...
Validation of Responsive Genes to Drought Stress in Two Contrasting Cultivars of Sugarcane
Sugarcane is a major crop worldwide, raw material for sugar and ethanol production. Drought is on... more Sugarcane is a major crop worldwide, raw material for sugar and ethanol production. Drought is on of the most limiting factor that affects sugarcane productivity. In order to understand the mechanisms of drought response, field and greenhouse essays were conducted with two drought-contrasting sugarcane genotypes (IACSP94-2094/tolerant and IACSP97-7065/sensitive). A microarray analysis, conducted with 14,522 genes using material grown on field, and a global gene expression analysis performed by RNAseq with plants grown in greenhouse have both identified several genes that are up/down-regulated under drought stress. Among these, nine genes (presented in both assays) were selected to be validated by qPCR using leaves. Four genes (Water Stress, Hormone Biosynthesis, Unknown1 and Unknown2) showed higher levels of transcripts in IACSP97-7065 in greenhouse, while levels in IACSP94-2094 were higher on field. Dehydrin and Cell Cycle genes showed the highest expression levels in sensitive gen...
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Papers by Rafael Junior