Papers by Juçara Paranhos
Iheringia, Série Botânica, 2018
Germinação in vitro de sementes e parâmetros morfofi siológicos de microestacas de Tabernaemontan... more Germinação in vitro de sementes e parâmetros morfofi siológicos de microestacas de Tabernaemontana catharinensis A. DC.
Análise da viabilidade polínica em acessos de Alternanthera tenella Colla
Anais Do Salao Internacional De Ensino Pesquisa E Extensao, 2011
Alternanthera tenella Colla (apaga-fogo), pertence a familia Amaranthaceae e e conhecida como uma... more Alternanthera tenella Colla (apaga-fogo), pertence a familia Amaranthaceae e e conhecida como uma especie medicinal, sendo sua parte aerea utilizada contra infeccoes bacterianas, como diuretica e antiinflamatoria. O presente trabalho objetivou analisar a estimativa da viabilidade polinica atraves de 2 tipos de corantes: orceina acetica 2% e reativo de Alexander. Foram utilizados botoes florais de 4 acessos de A. tenella, coletados em area urbana da cidade de Uruguaiana, RS. Para o estudo da viabilidade polinica, os botoes florais foram fixados em etanol-acido acetico (3:1) e mantidos em temperatura ambiente por 72 horas. Posteriormente, o material foi transferido para alcool 70% e conservado na geladeira. O preparo das lâminas foi realizado atraves da tecnica de esmagamento das anteras. Foram feitas 2 lâminas de cada acesso e utilizado o metodo de varredura contando 300 polens para cada tipo de coloracao por lâmina, estes foram analisados com auxilio de um microscopio com a objetiva no aumento de 40x. Quando corados com orceina acetica 2%, foram considerados viaveis os graos de polen bem corados e inviaveis os de coloracao fraca e/ou ausente. Quando corados com reativo de Alexander, foram considerados viaveis os graos de polen de coloracao arroxeada e inviaveis os de coloracao verde, verde azulado e parcialmente corados. Os resultados obtidos mostraram que acima de 92% dos graos de polen sao inviaveis para todos os acessos estudados, o que indica a baixa viabilidade polinica dos mesmos, nao havendo diferenca significativa entre os corantes testados.
Ciência Rural, 1994
RESUMO Estudou-se a indução de calos a partir de embriões maturos de arroz (Oryza sativa L.), cul... more RESUMO Estudou-se a indução de calos a partir de embriões maturos de arroz (Oryza sativa L.), cultivar BR-IRGA 414, cultivados em meio MS completo com diferentes concentrações de ANA e 2,4-D (0,5; 1,0; 5,0 e 10,0mg/l), acrescida ou não de cinetina (0,2mg/l), na presença e ausência de luz, os quais constituíram-se nos tratamentos. Nos dois estudos, o delineamento experimental foi inteiramente casualizado com cinco repetições e os tratamentos arranjados num trifatorial. Tanto ANA como 2,4-D foram efetivos na formação de calos. Não houve influência dos tratamentos adicionais no efeito das concentrações de ANA sobre o acúmulo da massa dos calos, embora tenha ocorrido aumento de 358% na massa quando se testou cinetina no escuro. Para 2,4-D, a massa dos calos decresceu com a presença de cinetina e luz.
Ciência e Natura, 2013
O objetivo deste trabalho foi selecionar isolados de Trichoderma spp. visando à sobrevivência de ... more O objetivo deste trabalho foi selecionar isolados de Trichoderma spp. visando à sobrevivência de mudas de feijoeiro (Phaseolus vulgaris L.) cv. carioca na presença de Sclerotinia sclerotiorum (Lib.) De Bary, bem como a promoção de crescimento desse cultivo. No experimento ex vitro, visando à sobrevivência de plantas de feijão, foram utilizados quatro tratamentos (presença dos isolados IT2, IT3 e IT4 de Trichoderma viride + controle sem Trichoderma spp.) em substrato Plantmax® inoculado com S. sclerotiorum. O experimento da promoção do crescimento de plantas de feijão foi conduzido na ausência e presença de pó biológico da mistura dos três isolados de T. viride (IT2, IT3 e IT4) em substrato esterilizado e não esterilizado, totalizando quatro tratamentos. Nos dois estudos, os trata-47-58, 2012/13 respectively). The misture of the three isolates of T. viride promoted the highest plants, either using sterilized substrate or not (23,69 cm and 20,42 cm, respectively) if compared to the treatments without isolates (13,78 cm and 16,375 cm, respectively). In the presence of biological powder the avarages of dry mass were higher (6,42 and 6,579 g) and different from the sterilized substrate without biological powder (3,98 g). We conclude that the isolates tested T. viride are effective in controlling S. sclerotiorum, presenting higher percentages of bean plants survivors. The mixture of isolates also promotes the growth of seedlings of bean cv. Carioca.
OBTENÇÃO DE BROTAÇÕES AÉREAS IN VITRO DE Rollinia salicifolia (SCHELCHT)
ufpel.tche.br
Page 1. R. bras. Agrociência, v. 10, n. 1, p. 131-133, jan-mar, 2004 131 OBTENÇÃO DE BROTAÇÕES AÉ... more Page 1. R. bras. Agrociência, v. 10, n. 1, p. 131-133, jan-mar, 2004 131 OBTENÇÃO DE BROTAÇÕES AÉREAS IN VITRO DE Rollinia salicifolia (SCHELCHT) OBTAINING IN VITRO AERIAL SPROUTINGS OF Rollinia salicifolia (SCHELCHT) ...
Biocell : official journal of the Sociedades Latinoamericanas de Microscopía Electronica ... et. al, 2013
Species of the genus Psychotria are used for multiple purposes in Brazilian folk medicine, either... more Species of the genus Psychotria are used for multiple purposes in Brazilian folk medicine, either as water infusions, baths or poultices. This study was aimed to evaluate the genotoxic and antiproliferative effects of infusions of Psychotria brachypoda and P. birotula on the Allium cepa test. Exposure to distilled water was used as a negative control, while exposure to glyphosate was used as a positive control. The interaction of extracts (as a post-treatment) with the effects of glyphosate was also studied. Results showed that glyphosate and the extracts of both P. brachypoda and P. birotula reduced the mitotic index as compared with the negative control (distilled water). Surprisingly, however, both extracts from P. brachypoda and P. birotula caused a partial reversal of the antiproliferative effect of glyphosate when used as a post-treatment. Glyphosate also induced the highest number of cells with chromosomal alterations, which was followed by that of P. birotula extracts. Howev...
Tree physiology, 2005
Psychotria umbellata Vell. (Rubiaceae), a Brazilian coastal woody species, produces umbellatine (... more Psychotria umbellata Vell. (Rubiaceae), a Brazilian coastal woody species, produces umbellatine (also known as psychollatine), an analgesic indole alkaloid. An in vitro embryogenic regeneration protocol capable of yielding alkaloid-accumulating plants was developed. Rhizogenic calli, which were obtained from stem segments derived from rooted apical cuttings, were cultured on Murashige and Skoog's (MS) medium containing either 1 mg l(-1) NAA (naphthalene acetic acid) and no kinetin, or 5 mg l(-1) NAA + 1 mg l(-1) kinetin. Calli did not accumulate umbellatine. Segments of rhizogenic callus were cultured on complete MS medium with various concentrations of kinetin and sucrose. Plant regeneration was best in the light with 0.25 mg l(-1) of kinetin and 1.5% sucrose. After 3 months of acclimatization in soil mixture, plant survival was 81%. Leaves of 10-month-old regenerated plants yielded umbellatine concentrations equivalent to those of adult forest-grown plants.
Phytochemistry, 2004
From leaves of Psychotria leiocarpa, an indole alkaloid was isolated to which the structure N,b-d... more From leaves of Psychotria leiocarpa, an indole alkaloid was isolated to which the structure N,b-d-glucopyranosyl vincosamide (1) was assigned. This represents the first report of an N-glycosylated monoterpenoid indole alkaloid. In field-grown plants highest amounts of 1 were found in the leaves (2.5% of dry wt) and fruit pulp (1.5% dry wt). Lower amounts were found in the stems (0.2% dry wt) and the seeds (0.1% of dry wt), whereas the alkaloid was not detected in the roots. The accumulation of 1 in aseptic seedlings was also restricted to the shoots and increased with plant age and light exposure, independent of the supply of sucrose in the culture medium.
OBTENÇÃO in vitro DE BROTOS DE Begonia rieger COM THIDIAZURON, CINETINA E ÁCIDO NAFTALENO ACÉTICO
Ciência Rural, 1994
Regeneração in vitro de Parapiptadenia rigida
Ciência Rural, 2009
ABSTRACT
Acúmulo de massa seca em três cultivares de arroz irrigado com diferentes arquiteturas de plantas
Ciência Rural, 1995
Biologia, 2008
Some species of Psychotria (Rubiaceae) are important in herbal medicine, where their extracts are... more Some species of Psychotria (Rubiaceae) are important in herbal medicine, where their extracts are used internally for infections of the female reproductive system, bronchitis, gastrointestinal disturbances, skin irritations, tumors, ulcers, and eye disturbances. The antiproliferative and genotoxic effects of Psychotria myriantha Mull. Arg. and P. leiocarpa Cham. et Schlecht infusions on the Allium cepa L. cell cycle were evaluated. The teas were prepared by infusing the leaves in distilled water, in two concentrations: 1.13 mg/mL and 6.78 mg/mL. Three groups of four bulbs were used for each Psychotria species. After the bulbs rooted in distilled water, they were transferred to the teas for 24 hours, except for the control that stayed in water. The rootlets were then collected, fixed in ethanol-acetic acid (3:1) for 24 hours, and stored in 70% ethanol. For each group of bulbs, 4000 cells were analyzed, calculating the mitotic indexes, submitting them to statistic analysis, using the χ 2 test (p = 0.05). The results showed a decrease in mitotic index with an increase in tea concentration in both species. In P. leiocarpa, the mitotic index values differed significantly between the control and concentration of 6.78 mg/mL (χ 2 = 9.863). For P. myriantha, the values referring to the mitotic index differed greatly between the control and the treatments (χ 2 = 124.8). With this study, it was determined that P. myriantha and P. leiocarpa infusions possess antiproliferative effects on the A. cepa cell cycle, and teas of P. myriantha also have genotoxic activity.
Biochemical Systematics and Ecology, 2009
Psychollatine is an unusual indole alkaloid produced by Psychotria umbellata Vell, an Atlantic Fo... more Psychollatine is an unusual indole alkaloid produced by Psychotria umbellata Vell, an Atlantic Forest understorey tree. Leaves, young inflorescences and fruit pulp displayed high amounts of psychollatine (from 2.5 to 4.5% dry wt). Seeds accumulated lower quantities (0.2% dry wt), and roots, trace amounts (0.06% dry wt). Alkaloid accumulation was not seasonally affected and leaf content was not significantly enhanced by wounding, exposure to salicylic acid, hydrogen peroxide, or UV. The alkaloid content in leaves decreased upon cutting exposure to auxin. Under strong UV, the amount of chlorophylls did not decrease compared to control contents at 48 and 72 h. Post-harvest psychollatine content in leaves was stable in different temperatures, except at 65 C. Psychollatine was an effective singlet oxygen, superoxide, and peroxide quencher. These antioxidant properties and the constitutively high amount of psychollatine indicate a role for the alkaloid in oxidative stress responses.
Journal of Natural Products, 2001
Brachycerine (1), an unusual alkaloid from the leaves of Psychotria brachyceras, was characterize... more Brachycerine (1), an unusual alkaloid from the leaves of Psychotria brachyceras, was characterized through spectroscopic data interpretation and its stereochemistry established by NOE difference techniques. Brachycerine (1) was found to be restricted to shoots in rooted cuttings of P. brachyceras (0.018 ( 0.004% dry weight), and accumulation was unaffected by root induction treatment with auxin.
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Papers by Juçara Paranhos