Papers by Antônio Carlos Martins Guedes
Revista da Sociedade Brasileira de Medicina Tropical, 2002
A case of HIV/Leishmania co-infection presenting both visceral and cutaneous manifestations is re... more A case of HIV/Leishmania co-infection presenting both visceral and cutaneous manifestations is reported. Leishmania infection was confirmed by conventional methods (parasitological approach and serology) and by PCR. Leishmania chagasi isolated from the skin lesion was characterized by enzyme electrophoresis and by restriction fragment length polymorphism of the internal transcribed spacer of the ribosomal gene.
Anais Brasileiros De Dermatologia, 1994
Memórias do Instituto Oswaldo Cruz, 2020
BACKGROUND Leprosy is an infectious-contagious disease caused by Mycobacterium leprae that remain... more BACKGROUND Leprosy is an infectious-contagious disease caused by Mycobacterium leprae that remain endemic in 105 countries. This neglected disease has a wide range of clinical and histopathological manifestations that are related to the host inflammatory and immune responses. More recently, the inflammasome has assumed a relevant role in the inflammatory response against microbiological agents. However, the involvement of inflammasome in leprosy remains poorly understood. OBJECTIVES The aim is to associate biomarkers of inflammasome with the different immunopathological forms of leprosy. METHODS We performed an observational, cross-sectional, and comparative study of the immunophenotypic expression of inflammasome-associated proteins in immunopathological forms of leprosy of 99 skin lesion samples by immunohistochemistry. The intensity and percentage of NLRP3, Caspase-1, Caspases-4/5, interleukin-1β and interleukin-18 immunoreactivities in the inflammatory infiltrate of skin biopsies were evaluated. FINDINGS Strong expression of NLRP3 and inflammatory Caspases-4/5 were observed in lepromatous leprosy (lepromatous pole). In addition, were observed low expression of caspase-1, interleukin-1β, and interleukin-18 in tuberculoid and lepromatous leprosy. The interpolar or borderline form showed immunophenotype predominantly similar to the lepromatous pole. MAIN CONCLUSIONS Our results demonstrate that the NLRP3 inflammasome is inactive in leprosy, suggesting immune evasion of M. leprae.
Anais Brasileiros de Dermatologia, 2013
Langerhans cell histiocytosis is a rare, clinically heterogeneous desease. Since there is conside... more Langerhans cell histiocytosis is a rare, clinically heterogeneous desease. Since there is considerable clinical overlap among the four described variants (Hand-Schüller-Christian, eosinophilic granuloma, Letterer-Siwe and Hashimoto-Pritzker), the concept of spectral disease applies to this entity. The Hashimoto-Pritzker variant was first described in 1973. Characteristically, it is present at birth or during the first days of life, impairment is limited to the skin and prognosis is favorable with spontaneous resolution. We report a newborn male patient with Hashimoto-Pritzker disease presenting as a S100 + and CD1a + single congenital perianal lesion with rapid involution in two months.
Anais Brasileiros de Dermatologia, 2008
Relata-se caso de leishmaniose tegumentar americana com lesões proeminentes na face. Os critérios... more Relata-se caso de leishmaniose tegumentar americana com lesões proeminentes na face. Os critérios diagnósticos incluíram dados clínicos e epidemiológicos, intradermorreação de Montenegro, identificação de Leishmania pela reação em cadeia da polimerase e resposta clínica ao tratamento. A importância do relato se deve ao fato de se tratar de caso incomum de leishmaniose tegumentar americana.
Acta Cirúrgica Brasileira
To evaluate a biofilm model of Pseudomonas aeruginosa in excisional cutaneous wound in mice. Meth... more To evaluate a biofilm model of Pseudomonas aeruginosa in excisional cutaneous wound in mice. Methods: Preclinical, translational study conducted with 64 C57BL/6 mice randomly assigned to control and intervention groups. Evaluation was on days D0, D3, D5, D7 and D10 of wound making. The profile of biofilm formation and induction was evaluated using wound closure kinetics, quantitative culture, and evaluation of wounds using transmission electron microscopy (TEM). Clinical evaluation was performed by liver tissue culture, weight variation, and quantification of leukocytes in peripheral blood. Analyses were performed with GraphPad Prism software. Results: Bacterial load for induction of infection with P. aeruginosa and survival of animals was 10 4 UFC•mL-1. In D5 (p < 0.0001) and D7 (p < 0.01), animals in the intervention group showed a delay in the healing process and had their wounds covered by necrotic tissue until D10. Statistical differences were observed in wound cultures and weight at D5 and D7 (p < 0.01). Liver cultures and leukocyte quantification showed no statistical differences. No bacteria in planktonic or biofilm form were identified by TEM. Conclusion: The findings raise questions about the understanding of the ease of formation and high occurrence of biofilm in chronic wounds.
Anais Brasileiros de Dermatologia, 2021
Infection with human papilloma virus (HPV) is related to a great number of cutaneous and mucosal ... more Infection with human papilloma virus (HPV) is related to a great number of cutaneous and mucosal manifestations. The spectrum of HPV ranges from inapparent infections, through various clinical benign presentations including cutaneous and mucosal disease, to malignant and premalignant conditions. New HPV types are currently described in the literature; many of them are characterized as high-risk types due to their oncogenic potential. Knowledge regarding their epidemiology and pathogenesis is important to understand not only infection and disease processes, but also to formulate the clinical and laboratory basis for diagnosis, therapeutics, and prophylactic measures. This non-systematic review aims to discuss and to update those aspects, with an emphasis on relevant topics for dermatologists. HPV infection and related diseases in the Brazilian scenario are highlighted, including common dermatologic conditions seen at clinics as well as the condition of a public health problem as a sexually transmitted infection. The oncogenicity of the virus and the variety of clinical outcomes-especially in the immunocompromised individuals-are addressed.
Anais Brasileiros De Dermatologia, 2021
PALAVRAS-CHAVE Infecções por papilomavírus; Infecções por papilomavírus, diagnóstico; Infecções p... more PALAVRAS-CHAVE Infecções por papilomavírus; Infecções por papilomavírus, diagnóstico; Infecções por papilomavírus, patologia; Infecções por papilomavírus, prevenção e controle; Infecções por papilomavírus, tratamento farmacológico; Papillomaviridae Resumo Nesta revisão não sistemática sobre o papiloma vírus humano (HPV) são abordados o diagnóstico complementar, o tratamento e as medidas de prevenção. Entre os métodos disponíveis para o diagnóstico complementar, discute-se a histopatologia, método consagrado na prática dermatológica, suas indicações, principais achados e limitações. Sobre a microscopia eletrônica, discute-se brevemente sua importância para os conhecimentos acumulados sobre o HPV e em pesquisas que buscam respostas que possam ser aplicadas na prática médica. São revistas as aplicações práticas do conhecimento molecular sobre o vírus em relação à sorologia, tipagem genômica e suas indicações. O enfoque na terapêutica é colocado nas opções disponíveis para tratamento da...
Anais Brasileiros De Dermatologia, 2021
Anais Brasileiros de Dermatologia, 2021
In this nonsystematic review, the complementary diagnosis, treatment, prevention, and control of ... more In this nonsystematic review, the complementary diagnosis, treatment, prevention, and control of human papillomavirus are discussed. The histopathology is addressed regarding its indications, main findings and limitations, as a complementary diagnostic method largely used by dermatologists. Electron microscopy is briefly reviewed, along with its contribution to the accumulated knowledge on HPV, as well as the relevance of research in using this technology for future advances in diagnosis and treatment. Molecular information about the virus is continuously increasing, and the practical applications of HPV serology, molecular identification and genotyping are discussed. Vaccines are a valuable tool in primary HPV infection prevention and are now available in many countries; their composition, indications, and adverse effects are revisited. Local and systemic treatment options are reviewed and off-label prescriptions are discussed. Finally, health education focusing on HPV infection as a sexually transmitted infection of worldwide relevance and the many barriers to improve primary and secondary prevention are addressed.
Arquivos Brasileiros de Oftalmologia, 1990
Leishmaniose cutânea disseminadairidociclite bilateral não granulomatosa. Isolamento de leishmani... more Leishmaniose cutânea disseminadairidociclite bilateral não granulomatosa. Isolamento de leishmania no humor aquoso. Disseminated cutaneous leishmaniasis-nongranulomatous bilateral iridocyclitis. Detection of leischmania in aqueous humor Fernando Oréfice(1) Sônia Regina A.A. Pinheiro(2) Teresa Cristina de A. Ferrari(3) Antônio Carlos M. Guedes(4) Odair Genaro(5) Marisa A. Marra(6) lara Lúcia N. Silveira(6) Tra baJJw realiwdo no Serviço de Uve(tes (Hos pital São Geraldo) e ClInica Médica do Faculda de deMedicina do U.F ,M.G. (1) Prof. Adj unto do ClInica Ofta lmo16gica (Hospital São Geraldo) do Faculdade de Me dicina do U.F,M.G. (2) Assistente voluntária do Serviço de Uve(te do Hospital São Geraldo, Faculdade de Medici na do U.F ,M.G. e Mestra em Oftalmo logia.
Journal of Wound, Ostomy & Continence Nursing, 2018
The purpose of this study was to investigate the effect of polyhexamethylene biguanide (PHMB) sol... more The purpose of this study was to investigate the effect of polyhexamethylene biguanide (PHMB) solution as a wound cleanser on bacterial load and bacterial biofi lm in venous leg ulcers. DESIGN: Randomized controlled trial. SUBJECTS AND SETTING: The target population was adults attending the dermatology outpatient clinic of the Clinical Hospital of the Federal University of Minas Gerais, Brazil. The sample comprised 44 patients with venous leg ulcers recruited over a 6-month period. METHODS: Participants were divided into 2 groups: the intervention group had their wounds cleansed with PHMB, and the control group had their wound cleansed with a 0.9% saline solution. Tissue fragments of the wounds were collected for bacteriological analysis; transmission electron microscopy was used to identify the presence of biofi lm. RESULTS: The bacterial load was reduced in both groups compared to baseline values; no signifi cant difference was found when groups were compared. Correlation analysis between wound duration (months), wound area (cm²), necrosis (%), variables, and bacterial count (colony forming units [CFUs]/g) after cleansing showed a signifi cant relationship between area of the wound and CFUs/g (P = .0070, r = 0.51). Transmission electron microscopy revealed the presence of bacterial biofi lm in the wounds after cleansing with both solutions. CONCLUSIONS: The results of this study indicate that both PHMB and saline solution are effective in reducing the bacterial load in venous leg ulcers. However, bacterial biofi lm was present after cleansing with both solutions. These fi ndings provide important evidence regarding effectiveness of 2 common wound cleansers on bacterial presence in wounds.
Revista Latino-Americana de Enfermagem, 2018
Objective: to identify factors associated with complex surgical wounds in the breasts and abdomen... more Objective: to identify factors associated with complex surgical wounds in the breasts and abdomen in outpatients. Method: observational case-control study involving 327 patients, distributed into 160 cases (complex surgical wound) and 167 controls (simple surgical wound). Data were extracted from the medical records and a binary logistic regression model was used for analysis, considering a significance level of 5%. Results: the factors associated with greater chance of occurrence of complex surgical wound were 18 to 59 years of age (p = 0.003), schooling < 8 years (p = 0.049), radiotherapy (p < 0.001), hysterectomy (p = 0.003), glycemia (≤ 99 mg/dL) and arterial hypertension (p = 0.033), while quadrantectomy (p = 0.025) served as a protective factor. Conclusion: radiotherapy was the most significant factor for surgical wound complications. Glycemic alteration was an unexpected result and shows the need for further studies related to this topic.
Anais brasileiros de dermatologia, 2018
Lobomycosis or lacaziosis is a chronic granulomatous fungal infection caused by Lacazia loboi. Mo... more Lobomycosis or lacaziosis is a chronic granulomatous fungal infection caused by Lacazia loboi. Most cases are restricted to tropical regions. Transmission is believed to occur through traumatic inoculation in the skin, mainly in exposed areas. It is characterized by keloid-like nodules. There are only a few hundred cases reported. The differential diagnoses include many skin conditions, and treatment is difficult. The reported case, initially diagnosed as keloid, proved to be refractory to surgical treatment alone. It was subsequently approached with extensive surgery, cryotherapy every three months and a combination of itraconazole and clofazimine for two years. No signs of clinical and histopathological activity were detected during follow-up.
Anais Brasileiros de Dermatologia, 2017
CASE REPORT This study is a case report of a 23-year-old woman with a medical condition of a diff... more CASE REPORT This study is a case report of a 23-year-old woman with a medical condition of a difficult to control eczema, which she has had since childhood, and a medical history of skin sepsis caused by Staphylococcus aureus (SA) in 2011. She presented erythematous-exudative plaques on the outer ear, armpits, groin, and inframammary region; flaky scalp; erythematous papules spread throughout the trunk and limbs; in addition to nasopharyngitis and scabs on the nostrils (Figures 1 and 2). The exams revealed an increased erythrocyte sedimentation rate (ESR). The culture of the secretion of the lesions revealed: Staphylococcus aureus, Klebsiella pneumoniae, and Acinetobacter baumannii multissensíveis; seropositive status for HTLV-1 (confirmed by Polymerase chain reaction-PCR). The skin biopsy showed hyperkeratosis, acanthosis, foci of spongiosis, corneal microabscesses, and perivascular and perifollicular lymphocyte infiltrate (Figure 3). The patient received cephalexin and a topical corticoid, presenting a significant improvement (Figure 4). After many recurrences, the chosen treatment was with an oral intake
Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, 1999
Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, 1999
... Gonçalves, DU; Guedes, AC; Soares, BC; Pinheiro, SR; Martins, ML; Murao, M; Cioffi, JM; Ribas... more ... Gonçalves, DU; Guedes, AC; Soares, BC; Pinheiro, SR; Martins, ML; Murao, M; Cioffi, JM; Ribas, JG; Proietti, FA; Carneiro-Proietti, AB; Lambertucci, JR; GIPH. Article Outline. Collapse Box ... April 5-9, 1999, Kagoshima, Japan. © 1999 Lippincott Williams &amp; Wilkins, Inc. ...
Jornal Brasileiro de Nefrologia, 2015
Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, 1999
International journal of rheumatology, 2011
Objective. Systemic sclerosis (SSc) is a complex autoimmune disease in which interferons (IFNs) m... more Objective. Systemic sclerosis (SSc) is a complex autoimmune disease in which interferons (IFNs) may play an essential role. We hypothesized that type I and III IFNs may be found in increased levels in patients and be responsible for SSc autoimmune status. Methods. Type I and III IFN and ISG basal expression profiles were measured by qPCR using RNA from PBMCs of patients and controls . Results. Type I IFNs are increased in SSc patients, while no induction of type III IFNs was detected. This induction cannot be related to IRF7, since no upregulation of this gene was seen on patients. Of the ISGs tested, 2'5'OAS levels were increased in patients, while 6-16 and MxA levels were not. Conclusions.…
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Papers by Antônio Carlos Martins Guedes