Papers by Caren Nádia Soares De Sousa
Brazilian Journal of Medical and Biological Research, 2021
Ketamine (KET) is an N-methyl-D-aspartate (NMDA) antagonist with rapid and long-lasting antidepre... more Ketamine (KET) is an N-methyl-D-aspartate (NMDA) antagonist with rapid and long-lasting antidepressant effects, but how the drug shows its sustained effects is still a matter of controversy. The objectives were to evaluate the mechanisms for KET rapid (30 min) and long-lasting (15 and 30 days after) antidepressant effects in mice. A single dose of KET (2, 5, or 10 mg/kg, po) was administered to male Swiss mice and the forced swim test (FST) was performed 30 min, 15, or 30 days later. Imipramine (IMI, 30 mg/kg, ip), a tricyclic antidepressant drug, was used as reference. The mice were euthanized, separated into two time-point groups (D1, first day after KET injection; D30, 30 days later), and brain sections were processed for glycogen synthase kinase-3 (GSK-3), histone deacetylase (HDAC), brain-derived neurotrophic factor (BDNF), and glial fibrillary acidic protein (GFAP) immunohistochemical assays. KET (5 and 10 mg/kg) presented rapid and long-lasting antidepressant-like effects. As expected, the immunoreactivities for brain GSK-3 and HDAC decreased compared to control groups in all areas (striatum, DG, CA1, CA3, and mainly pre-frontal cortex, PFC) after KET injection. Increases in BDNF immunostaining were demonstrated in the PFC, DG, CA1, and CA3 areas at D1 and D30 time-points. GFAP immunoreactivity was also increased in the PFC and striatum at both time-points. In conclusion, KET changed brain BDNF and GFAP expressions 30 days after a single administration. Although neuroplasticity could be involved in the observed effects of KET, more studies are needed to explain the mechanisms for the drug's sustained antidepressant-like effects.
A investigação de paternidade no direito brasileiro
Revista Do Centro Academico Afonso Pena, 1999
P.4.C.005 Effect of flavonoids isolated from Erythrina velutina on oxidative stress in mouse brains
European Neuropsychopharmacology, Oct 1, 2014
036 — (LIM0129) Anticonvulsant activity of acute treatment with manidipine in the electroshock-induced seizure model in mice
Epilepsy & Behavior, Sep 1, 2014
Brazilian Journal of Medical and Biological Research, 2022
The use of bladder antimuscarinics is very common in the elderly. However, recent population-base... more The use of bladder antimuscarinics is very common in the elderly. However, recent population-based studies that assessed the use of anticholinergics or bladder antimuscarinics showed an increased risk of dementia when these drugs were used for a prolonged period. Several of these population-based studies included patients who used solifenacin, which is a bladder antimuscarinic released in 2005 with the prospect of being a more selective antimuscarinic for M3 receptors (M3R), which could make it a safer drug when trying to avoid unwanted effects of older bladder antimuscarinics such as oxybutynin, especially with regard to changes in cognition. Since the various bladder antimuscarinics have distinct pharmacological characteristics, such as in the ability to penetrate the blood-brain barrier, in selectivity for muscarinic receptors, and in brain efflux mechanisms, their effects on the central nervous system (CNS) may vary. Solifenacin was the drug selected in this review, which aims to describe the results of several articles published in recent years reporting the effects of solifenacin on cognition or the risk of dementia development. Although preclinical studies show that solifenacin can also act on brain M1 receptors (M1R), short-term clinical studies have shown it to be safe for cognition. However, there are no long-term randomized studies that prove the safety of this drug for the CNS. Thus, until the safety of solifenacin has been established by long-term studies, it seems advisable to avoid prolonged use of this drug in elderly patients.
040 — (MAI0020) Investigation of NO–cGMP pathway involvement in an animal model of seizures induced by pilocarpine
Epilepsy & Behavior, 2014
036 — (LIM0129) Anticonvulsant activity of acute treatment with manidipine in the electroshock-induced seizure model in mice
Epilepsy & Behavior, 2014
P.4.C.005 Effect of flavonoids isolated from Erythrina velutina on oxidative stress in mouse brains
European Neuropsychopharmacology, 2014
European Neuropsychopharmacology, 2014
50 mM for U251 and U87 cell lines and 100 mM for C6 cell line). When applied in its IC50 concentr... more 50 mM for U251 and U87 cell lines and 100 mM for C6 cell line). When applied in its IC50 concentration, desloratadine increased production of ROS in U251 (1.4 times, compared to the control) as well as in other cell lines. It was also noted an increased caspase activity which implies to caspase-dependent apoptosis. Using double staining with Annexin V (An) and propidium iodide (PI), other apoptotic marker (extravasation of phosphatidyl serine) was noted after the tretment (33% of apoptotic cells in comparison to control that had 1.7%). Cell cycle analysis showed higher percent of apoptotic cells in sub G 0 phase in C6 cell line (increased FL2 fluorescence). Increased presence of the acid cytoplasmic vesicles (quantified as an increase of the orange-FL3 vs. green-FL1 fluorescence, compared to the control) indicated a presence of autophagy in all investigated cell lines. Immunoblot method revealed the increased expression of LC3II after 18 h and 24 h of treatment, in dose-dependent manner. Conclusion: Desloratadine exerts cytotoxic effects on exammined cell lines, inducing apoptosis and autophagy. On view of the results showen above, we can conclude that its cytotoxic effect is not cell type speciffic.
European Neuropsychopharmacology, 2014
Oxidative Medicine and Cellular Longevity
Anxiety disorders are the most prevalent psychiatric disorders being also a comorbid state of oth... more Anxiety disorders are the most prevalent psychiatric disorders being also a comorbid state of other diseases. We aimed to evaluate the anxiolytic-like effects of carvedilol (CVD), a drug used to treat high blood pressure and heart failure with potent antioxidant effects, in animals exposed to chronic unpredictable stress (CUS). To do this, female Swiss mice were exposed to different stressors for 21 days. Between days 15 and 21, the animals received oral CVD (5 or 10 mg/kg) or the antidepressant desvenlafaxine (DVS 10 mg/kg). On the 22nd day, behavioral tests were conducted to evaluate locomotor activity (open field) and anxiety-like alterations (elevated plus-maze—EPM and hole board—HB tests). After behavioral determinations, the animals were euthanized, and the adrenal gland, blood and brain areas, prefrontal cortex (PFC), and hippocampus were removed for biochemical analysis. CUS reduced the crossings while increased rearing and grooming, an effect reversed by both doses of CVD a...
Lectin isolated from the red marine alga Solieria filiformis (Kützing) P.W. Gabrielson: Secondary structure and antidepressant-like effect in mice submitted to the lipopolysaccharide-induced inflammatory model of depression
Algal Research
Cuidado integral em saúde, 2020
Copyright © do autor e da autora. Todos os direitos garantidos. Este é um livro publicado em aces... more Copyright © do autor e da autora. Todos os direitos garantidos. Este é um livro publicado em acesso aberto, que permite uso, distribuição e reprodução em qualquer meio, sem restrições desde que sem fins comerciais e que o trabalho original dos autores e autoras seja corretamente citado.
Involvement of oxidative pathways and BDNF in the antidepressant effect of carvedilol in a depression model induced by chronic unpredictable stress
Psychopharmacology, 2022
RATIONALE Depression is a severe psychiatric disorder with oxidative imbalance and neurotrophic d... more RATIONALE Depression is a severe psychiatric disorder with oxidative imbalance and neurotrophic deficits as underlying mechanisms. OBJECTIVES Based on the antioxidant effects of carvedilol (CARV), here, we aimed to evaluate CARV's effects against depression induced by the chronic unpredictable stress (CUS) model. METHODS Female Swiss mice were submitted to the CUS protocol for 21 days. Between days 15 and 22, the animals received CARV (5 or 10 mg/kg) or desvenlafaxine (DVS 10 mg/kg) orally. On the 22nd day, mice were subjected to behavioral tests to evaluate locomotion, depressive-like behavior (tail suspension test), motivation/self-care with the splash test (ST), social interaction, and working memory Y-maze test. The prefrontal cortex (PFC) and hippocampus were dissected to evaluate alterations of oxidative and brain-derived neurotrophic factor (BDNF). RESULTS The CUS model reduced locomotion and increased grooming latency, while it reduced the number of groomings in the ST. Both doses of CARV and DVS reverted these alterations. In addition, DVS and CARV reversed CUS model-induced working memory and social interaction deficits. The CUS model decreased hippocampal reduced glutathione (GSH), while DVS and CARV increased GSH in the PFC (CARV5) and hippocampus (CARV5 and 10). The CUS model increased nitrite and malondialdehyde (MDA) concentrations in both areas. All treatments reversed nitrite alterations, while CARV10 changed MDA levels in PFC and all treatments in the hippocampus. The CUS model reduced BDNF levels. CARV10 increased BDNF in the PFC, while both doses of CARV increased hippocampal levels of this neurotrophin. CONCLUSIONS CARV presents antidepressant-like effects comparable to those observed with DVS. In addition, it has an antioxidant effect and is capable of increasing BDNF brain concentrations. Further studies are needed to elucidate the mechanisms involved in the antidepressant effect of CARV.
Iconography : Effects of standard ethanolic extract from Erythrina velutina in acute cerebral ischemia in mice
Estratégia educativa como tecnologia facilitadora para promoçao da saúde do adolescente no âmbito escolar
A depressao e um transtorno psiquiatrico grave e incapacitante que atinge pessoas de todas as cla... more A depressao e um transtorno psiquiatrico grave e incapacitante que atinge pessoas de todas as classes socioeconomicas, etnias e idades. O estresse oxidativo, a neuroinflamacao e o deficit de fatores neurotroficos tem se destacado como justificativa para os sintomas e falha terapeutica na depressao. Baseado no pressuposto de que o carvedilol (CVD) apresenta propriedades antioxidantes e anti-inflamatorias, objetivou-se avaliar os efeitos comportamentais e neuroquimicos da administracao repetida de CVD, no modelo de depressao, induzido por estresse cronico imprevisivel (ECI). Para a inducao de depressao camundongos Swiss femeas (30-32g) foram submetidos, durante 21 dias, a diferentes estressores. Entre os dias 15 e 21 os animais receberam CVD (5 ou 10 mg/Kg) ou desvenlafaxina (DVS 10 mg/Kg) por via oral. No 22o dia os animais foram submetidos aos seguintes testes comportamentais: campo aberto, labirinto em cruz elevada, placa perfurada, suspensao de cauda, borrifagem de sacarose, inter...
Efeitos comportamentais e neuroquímicos de ácido alfa-lipóico e desvenlafaxina em modelo animal de depressão induzido por corticosterona
A depressao e um transtorno psiquiatrico grave e incapacitante que atinge pessoas de todas as cla... more A depressao e um transtorno psiquiatrico grave e incapacitante que atinge pessoas de todas as classes socioeconomicas, etnias e idades. Estudos recentes tem sugerido um possivel envolvimento do estresse oxidativo na patogenese da depressao e que substâncias com potencial antioxidante podem ser utilizadas no tratamento desse transtorno. Baseado neste pressuposto, o presente estudo investigou os efeitos comportamentais e neuroquimicos da administracao repetida de Acido α-Lipoico (ALA), sozinho ou associado a Desvenlafaxina (DVS), no modelo de depressao induzido pela administracao cronica de Corticosterona (CORT). Foram utilizados camundongos Swiss femeas (25-30g) e estes receberam, por via subcutânea, veiculo ou CORT 20 mg/kg durante 21 dias. Outros grupos receberam veiculo ou CORT durante 14 dias e, do 15o ao 21o dia de tratamento, DVS 10 ou 20 mg/kg, ALA 100 ou 200 mg/kg, ou as combinacoes DVS10 + ALA100, DVS20 + ALA100, DVS10 + ALA200 ou DVS20 + ALA200. Uma hora apos a ultima admin...
Early maternal separation enhances melanoma progression in adult female mice by immune mechanisms
Annals of the New York Academy of Sciences, 2021
Maternal separation (MS) is a risk factor for major depressive disorder. Both cancer and depressi... more Maternal separation (MS) is a risk factor for major depressive disorder. Both cancer and depression seem to share a common biological link. Here, we evaluated the progression of melanoma and the underlying mechanisms related to this progression, namely cell proliferation and apoptosis, in adult female mice exposed to MS. Female C57BL/6 mice were exposed to MS for 60 min/day during the first 2 postnatal weeks (here called MS mice) or left undisturbed (here called non‐MS mice). Melanoma cells were inoculated subcutaneously into the axillary region of adult animals, and tumor progression was evaluated for 25 days. Adult MS mice presented depressive‐like behavior and working memory deficits. MS accelerated murine melanoma growth by mechanisms related to decreased apoptosis and increased cell proliferation rate, such as increased expression of IL‐6 and mTOR. MS stimulated eukaryotic elongation factor 2 expression and increased the number of circulating monocytes and DNA damage in peripheral blood leukocytes, an effect associated with oxidative DNA damage. In conclusion, MS accelerated the progression of murine melanoma by mechanisms related to tumor proliferation and apoptosis, revealing a relationship between adverse childhood experiences and cancer progression, particularly melanoma.
Brazilian Journal of Health Review, 2021
Câncer infantil é todo e qualquer tipo de processo patológico que desencadeia um neoplasma malign... more Câncer infantil é todo e qualquer tipo de processo patológico que desencadeia um neoplasma maligno e que se manifesta em crianças menores de 15 anos. Além dos prejuízos acarretados ao público infanto-juvenil, o diagnóstico do câncer origina malefícios também aos cônjuges. Neste contexto, o diagnóstico Processos Familiares Interrompidos mostra-se uma importante ferramenta na assistência aos pais de crianças diagnosticadas com câncer. O presente estudo objetivou investigar a possível presença de tal diagnóstico em pais de crianças diagnosticadas com câncer e internadas para tratamento em uma unidade de referência. Estudo descritivo, com abordagem quantitativa e qualitativa, do tipo transversal. Os critérios de inclusão utilizados foram: consentimento das participações, maioridade e acompanhamento da rotina de tratamento do filho. 100% dos entrevistados apresentaram uma característica definidora ou um fator relacionado presentes no diagnóstico de enfermagem investigado. Ao longo do estudo, surgiram cinco categorias, a saber: mudanças no relacionamento conjugal; alterações nos processos de comunicação; mudanças na vida sexual do casal; modificações no convívio do casal; expressões de sentimentos. Dentre os achados observados ao longo deste estudo, destaca-se a quantidade de relatos da existência de mudanças abruptas na rotina dos pais das crianças hospitalizadas, evidenciando deste modo a presença do diagnóstico estudado em todos os participantes.
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Papers by Caren Nádia Soares De Sousa