Faculdade De Engenharia

During semen cryopreservation, sperm cells were submitted to several deleterious events leading to membrane damage which result in fertility decrease. This study was designed to compare the effects of two freezing techniques (conventional and automated), and the use of two commercial extenders as cryoprotectants (FR-5® and Botu-Crio®) on total and progressive motility, integrity and functionality of spermatic membranes during the cryopreservation of equine semen. Twenty ejaculates from two stallions were analyzed. The total and progressive motility of fresh and post-thawing semen samples were evaluated by patterns assays. Function of plasmatic membrane was measured by the hipoosmotic swelling test. Integrity of plasmatic membrane was evaluated using carboxifluorescein diacatate and iodidium propide fluorescent probes. There were significant differences between the two freezing techniques and/or between cryoprotectants for all assessed parameters. The combination of Botu-Crio® and automated curves showed better results on total and progressive post-thawing motility. The extender Botu-Crio®, alone, showed to better preserve the membrane integrity and function.

Ruído Cansaço, irritação, dores de cabeça, surdez temporária ou permante, redução da concentração, desconforto Vibrações Cansaço, irritação, dores nos membros, dores na coluna, redução da concentração, desconforto Radiações Alterações celulares, câncer, fadiga, queimaduras, lesões nos olhos e na pele . Humidade Doenças no aparelho respiratório, quedas Elevadas/baixas temperaturas Desidratação, queimaduras, fadiga, doenças no aparelho respiratório Agentes Físicos