Papers by Wimpie Pangkahila
Nutrilite green trim® reduced body weight, waist circumference, and body fat percentage in obese men with moderate physical exercise and low-calorie diet
Jurnal Biomedik : JBM, Mar 30, 2017
This study was aimed to prove that oral administration of ALA and moderate physical exercise decr... more This study was aimed to prove that oral administration of ALA and moderate physical exercise decrease body weight and abdominal fat more than moderate physical exercise only in obese male Wistar rats. This was a true experimental study with a post-test only control group design. Subjects were 30 Wistar rats (Rattus norvegicus), male, healthy, aged 4-5 months, and obese with minimum weight of 250 g, divided into 3 groups, as follows: 1) P0, the control group with no treatment; 2) P1, given moderate physical exercise (swimming for 20 minutes/day, for 4 weeks); 3) P2, given moderate physical exercise and ALA per oral 15 mg/day for 4 weeks. The results showed that the average body weight after 4 weeks of treatment of P0 group was 279.10±5.84 g; of P1 group was 257.90±10.31 g; and of P2 group was 213.90±8.92 g (P < 0.01). The mean weight of subcutaneous abdominal fat of P0 group was 0.96±0.45 g; of P1 group was 0.63±0.31 g; and of P2 group was 0.40±0.23 g (P < 0.01). Moreover, the mean weight of visceral abdominal fat of P0 group was 1.23±0.37 g; of P1 group was 0.83±0.24 g; and of P2 group was 0.39±0.25 g (P < 0,01). Conclusion: Oral administration of ALA and moderate physical exercise decrease body weight, subcutaneous abdominal fat, and visceral abdominal fat more than moderate physical exercise only in obese male Wistar rats.
Indonesian Journal Of Global Health Research, Nov 28, 2019
As the population of elderly increases, the number of andropause also increases. Andropause is ch... more As the population of elderly increases, the number of andropause also increases. Andropause is characterized by the decrease of testosteron level which is accompanied by hypogonadism symptoms. Many studies mentioned that decreased testosterone level will contribute to inflammation state, one of the cytokine is TNF-α, which then lead to many chronic inflammation disorders and influence the quality of life. Understanding the significant correlation between androgen and inflammation allows accurate consideration making in using androgen replacement therapy, therefore the quality of life will be improved significantly.This research was an analytical cross-sectional study. Subjects were recruited through random sampling at Denpasar in 2019. TNF-α serum level was measured by human immunoassay method. Andropause was measured by ADAM questionnaire and confirmed by total decreased testosterone serum level. Quality of life was measured by WHOQOL-BREF questionnaire which was then continued by bivariate and multivariate analysis to control the confounding variables. It was considered statistically significant if P value is more than 0.05. There were 60 elderly involved as research subjects consisting of 43 andropause cases and 17 non andropause cases. There was significant correlation between TNF-α serum and quality of life. This study also found that there was significant difference in quality of life between andropause and non andropause group. This study shows that there was significant correlation between TNF-α serum level and andropause with quality of life in elderly.
International journal of scientific advances, 2023
Background: The leaf of sensitive plant (Mimosa pudica Linn) has a fairly high content of flavono... more Background: The leaf of sensitive plant (Mimosa pudica Linn) has a fairly high content of flavonoids, which work in inhibiting the tyrosinase enzyme. Changes in the formation of this enzyme will cause the melanin pigment to be disrupted, resulting in the appearance of skin pigmentation disorders (hyperpigmentation). The purpose of this study was to prove the effectiveness of 15% sensitive plant leaf extract cream on guinea pig skin exposed to ultraviolet B (UVB). Methods: This is true experimental research using randomized post-test only control group design. The subjects were 30 male guinea pigs randomly divided into 3 groups, namely the control group (K), treatment group 1 (P1), and treatment group 2 (P2). Control group was not given any treatment, P1 was smeared with basic cream and exposed to UVB and P2 was smeared with 15% sensitive plant leaf extract cream and exposed to UVB for 2 weeks. After 2 weeks, the tyrosinase enzyme level were examined using ELISA and melanin amount were examined using Masson Fontana staining. The result was analyzed using comparative test between groups. Results: The tyrosinase enzyme in P2 group was 5,440,63 ng/ml, which is lower than K group (8,600,16 ng/ml (p<0,001)) and P1 (12,96±2,21 ng/ml (p<0,001)). The mean melanin amount in P2 was 0,090,07 %, which was also lower than K (0,310,24 % (p=0,049)) and P1 (0,53±0,47 % (p=0,043)). Conclusion: Administration of sensitive plant leaf extract cream inhibited the increase in tyrosinase enzyme and melanin amount in guinea pig exposed to UVB and normal subjects.
International journal of research publications, May 16, 2023
Background: Melanin is produced through melanogenesis which involves several molecular processes ... more Background: Melanin is produced through melanogenesis which involves several molecular processes with microphthalmia-associated transcription factor (MITF) as the main regulator of melanin synthesis. Hyperpigmentation is one of aging sign that happens due to melanin buildup in the skin. Hyperpigmentation can be caused by many factors, and one of the most frequent cause is ultraviolet (UV) ray exposure. Robusta coffee silverskin is a thin layer on a green coffee bean that is detached from the bean through roasting process, and is a waste product of coffee production. However, coffee silverskin contains high amount of phenolic and flavonoid compounds, and is categorized as strong antioxidant. The purpose of this study was to prove that administration of 30% Robusta coffee silverskin ethyl acetate fraction cream inhibited the increase of MITF expression and melanin amount in the skin of male adult guinea pig exposed to UVB ray. Methods: This study used randomized post-test only control group design. Subjects used in this study were healthy male guinea pigs with age ranging from 3-4 months and average weight 300-350g. Total samples used in this study were 36 guinea pigs divided evenly into two groups, Group K that were only exposed to UVB ray, and Group P that were exposed to UVB ray and 30% Robusta coffee silverskin ethyl acetate fraction cream. Total UV ray used during two weeks were 390mJ/cm 2. 30% Robusta coffee silverskin ethyl acetate fraction cream was applied twice a day on guinea pigs at the days they were not exposed to UVB ray, and at the days they were exposed to UVB ray, the cream was applied 20 minutes before and 4 hours after the exposure. MITF expression was examined under the microscope with MITF antibody kit, and melanin amount was examined with Masson-Fontana staining. Results: Result of the experiment showed higher MITF expression and melanin amount in the Group K.
Administration of Oral Sorghum Seed Extract (Sorghum bicolor L.) Increased Pancreatic Beta Cells Count but did not Reduce Glycated Albumin Levels in Male Wistar Rats (Rattus Norvegicus) with Diabetes Mellitus
Background: It is required a long time of diabetes mellitus treatment, such as doing general acti... more Background: It is required a long time of diabetes mellitus treatment, such as doing general activities or even insulin. Sorghum is a plant that contains antioxidants. The aim of this research was to prove the oral administration of Sorghum Seed Extract (Sorghum bicolor L.) in increasing the pancreatic beta cells count but did not reduce the levels of glycated albumin in male Wistar rats (Rattus norvegicus) with Diabetes Mellitus. Method: This study used experimental post-test only group design. 36 male Wistar rats were used and injected with the nicotinamide and streptozotocin intraperitoneally. Rats were divided into 2 groups, the control group (n=18) was administrated with distilled water at a dose of 2cc/200g body weight of rats. The the treatment group (n=18) was administrated with ethanol extract of sorghum seeds with a dose of 80 mg/kgBW of rats/ day. The post-test of glycated albumin levels and histopathology examination of pancreatic beta cells count were performed. Results: The mean of pancreatic β cells count in the control group was 64.31 ± 19.397. It was lower than the treatment group which was 119.44 ± 33.799 (p 0,05), therefore it was not proven. Conclusion: The administration of ethanol extract of sorghum seeds increased pancreatic β cells count, but it did not reduce glycated albumin levels in male Wistar rats (Rattus Norvegicus) with Diabetes Mellitus.
Topical bromelain cream did not increase neovascularization, fibroblast and epithelialization in wound healing of diabetic-rats (rattus norvegicus)
Introduction: The diabetic-wound healing process is longer due to blood vessel ischemia. Bromelai... more Introduction: The diabetic-wound healing process is longer due to blood vessel ischemia. Bromelain is the enzyme that acts as debridement agent with anti-inflammatory effect. The aim of this study was to prove the topical administration of bromelain increase neovascularization, fibroblast, and epithelialization in wound healing of diabetic-rats. Methods: This study used post-test only control group design. Subjects were 32 male Wistar diabetic-rats (Rattus norvegicus. It was divided into 2 groups (n = 16) and given amoxiciline for 3 days. The control group consist of: O1 group treated with a base cream for 5 days and O2 group was treated for 12 days. While the treatment group consist of: O3 group was treated with 70% bromelain cream for 5 days and O4 group was treated for 12 days. The neovascularization, fibroblasts, and epithelialization were examined histopathologically. Results: The mean number of fibroblasts in O1 and O3 were 222.88±37.94 cells/field of view and 25.38±7.94 cells/field of view, while O2 and O4 were 99.63±82.33 cells/field of view and 32.25±12.453 cells/field of view (p 0,05). Conclusion: Topical bromelain cream 70% did not increase neovascularization, fibroblast, and epithelialization in wound healing of diabetic-rats.
Introduction: Dyslipidemia is one of the most prevalent health conditions and often leads to deve... more Introduction: Dyslipidemia is one of the most prevalent health conditions and often leads to development of many chronic diseases such as coronary artery disease, heart failure, and stroke. This study was conducted to investigate the effects of bitter gourd (Momordica Charantia) extract on lipid profiles in male wistar rats fed with high-fat diet. Methods: This study was an experimental research using randomized post-test only control group design. The subjects were 36 male Wistar rats (Rattus norvegicus) randomly divided into 2 groups. The first group was the control group (P0) and fed with placebo while the treatment group (P1) received bitter gourd (Momordica Charantia) ethanol extract at 1g/kg body weight/day. Both groups were fed with high fat diet. The experiment was lasted for 35 days and lipid profiles were measured at the end of the experiment. Results: Statistical analysis results revealed the mean of total cholesterol, triglyceride, and low-density lipoprotein (LDL) in the treatment group were significantly lower than control group while high-density lipoprotein (HDL) was significantly higher in the treatment group (p<0.05). This study concluded that bitter gourd extract significantly prevent dyslipidemia on male Wistar rats fed with high-fat diet. Bitter gourd,ethanol extract, lipid profiles, dyslipidemia
Neurologico Spinale Medico Chirurgico, Mar 31, 2021
Background: This study aimed to prove that administration of UltraMimi Kids® UHT milk by PT. Ultr... more Background: This study aimed to prove that administration of UltraMimi Kids® UHT milk by PT. Ultrajaya Milk Industry increased body weight and estrogen levels in female albino rat (Rattus norvegicus) Wistar strain. Methods: This research was a true experimental with randomized pretest-posttest control group design. The research subjects were 18-female-albino-rats aged 21-days, weight 25-28 grams, divided into 2-groups: control group which was given standard food and sugar solution (0.36 grams of sugar dissolved in 1 ml aquadest), and intervention group which was given standard food and UltraMimi Kids® UHT milk 1 ml for the first 14-days and upgraded to 2 ml for the rest 14-days, given three times a day for 28-days. The rats body weight and estrogen levels were measured using Tanita-KD-192-digital-scales and ELISA method. Results: The control group had an increased body weight (26.2±1.2-66.1±5.94 grams; p<0,001), but there was no change in estrogen levels (38.59±11.19-44.59±5.42 ng/L; p=0.217). Whereas, the intervention group had an increased body weight (25.77±0.83-85.33±10.24 grams; p=0.008), but there was no change in estrogen levels (39.91±3.73-42.66±2.24 ng/L; p=0.066). After 28-days of intervention (posttest), it was found that the mean of body weight in the intervention group was higher than the control group (p<0.001), there was no significant difference in estrogen levels (p=0.339) and food intake from the remaining feed in both groups (0.51±0.81-0.36±0.55 grams; p=0.823). Conclusion: The administration of UltraMimi Kids® UHT milk by PT. Ultrajaya Milk Industry increased body weight and did not increase estrogen hormone level in female albino rat (Rattus norvegicus) Wistar strain.
Neurologico Spinale Medico Chirurgico, Mar 31, 2021
Background: Oxidative stress plays a role in the obesity mechanism, thus leads to premature aging... more Background: Oxidative stress plays a role in the obesity mechanism, thus leads to premature aging. High antioxidant capacity in Bajakah tampala stem may effectively lessen oxidative stress and reduce fat mass and body weight accordingly. This study aimed to provide Bajakah tampala stem extract's effect in lowering ROS level, visceral fat weight, and overall weight of obese male Wistar rats. Method: A true experimental design was conducted on male Wistar rats aged 2-3 months with obesity. Thirty-two obese rats were evenly divided into a placebo group and a group given Bajakah tampala stem extract, with 16 rats in each group. For 28 days, both groups were fed a high-fat diet. The subject body weights were weighed every week. ROS levels and visceral fat weight were evaluated after the intervention was done. Comparative analysis between groups was performed. Results: The results showed mean levels of ROS (56.2 ± 7.4 U/ml vs. 400.9 ± 50.7 U/ml; p < 0.001), visceral fat weight (2.6 ± 0.2 g vs. 3.4 ± 0.9 g; p < 0.001), and the final body weight (241.5 ± 2.8 g vs. 261.5 ± 13.8 g; p < 0.001) were significantly lower in the study group than the control group. Conclusion: The study indicates Bajakah tampala stem extract administration effectively reduced ROS levels, visceral fat weight, and body weight in obese male Wistar rats.
Administrations of young betelnut fruit extract (Areca cathecu l.) increased testosteron concentration in old male wistar white rat (Rattus norvegicus)
Background: With increasing age, testosterone level is tend to and cause sexual dysfunction disor... more Background: With increasing age, testosterone level is tend to and cause sexual dysfunction disorders such as erectile dysfunction, sexual arousal disorders, ejaculatory disorders, orgasm disorders to infertility. Young betel nut extract (Areca catechu L.) is an herbal plant that can increase sexual arousal (libido) which has many nutrients including antioxidants. The purpose of this study was to prove that administration of young areca fruit extract could increase testosterone levels in old male wistar white rats (Rattus norvegicus). Method: This study was an experimental study using a pretest-posttest control group design that used 16 wistar male rats (Rattus norvegicus) aged 18 months old, weighing 225-250 grams divided into 2 (two) groups, namely the control group (Placebo ) and the treatment group who were given young betel nut extract 100 mg / kgBB / day (P1). The study was conducted for 28 days followed by blood sampling for testosterone levels. Results: The results showed significant increase in testosterone levels in treatment group compared to the control group, (p= <0.05)). The testosterone level increased from 14.813 ± 1.343 ng/ml before treatment to 19.602 ± 1.345 ng/ml after treatment. Conclusion: Based on the results of the analysis it can be concluded that the administration of Areca catechu L. extract can increase Testosterone levels in old male wistar white rats (Rattus norvegicus).
Introduction: Aging can cause decreased testosterone levels in men which is known as andropause. ... more Introduction: Aging can cause decreased testosterone levels in men which is known as andropause. Some herbs are known to increase the levels Testosterone. One of them is Klabet seed that contain phyto-testosterone. The purpose of this study was to prove that the administration of ethanol extract of Klabet seeds increased testosterone levels in old male Wistar rats. Methods: A pretest-posttest control group design study was conducted using 20 male white wistar rats that were divided into 2 (two) groups: control group that received 2 cc of distilled water and treatment group that received 2 cc ethanol extract of Klabet seeds 250mg/250gBB rats/day by sonde for 14 days. The testosterone level was measured on day 1 and 15. Results: The analysis showed that there was no significant difference in the pre-test and post-test testosterone level P0 (Pre-test:16.4±0.553 ng/ml; posttest: 15.9±1.51ng/ml) while increased level of testosterone could be observed in treatment group (P1) (pre-test: 16.6±0.476ng/ml; post-test: 19.4±0.828ng/ml. Comparative analysis between post-test results (P0 vs P1) showed that the difference was statistically significant (p <0.001). Conclusion: Oral administration of Klabet seeds ethanol extract can significantly increase testosterone level in old male wistar mice.
Evaluation of transurethral application of alprostadil for erectile dysfunction in Indonesians
PubMed, Sep 1, 2000
Aim: To evaluate the efficacy and safety of transurethral application of alprostadil (MUSE) for t... more Aim: To evaluate the efficacy and safety of transurethral application of alprostadil (MUSE) for the treatment of erectile dysfunction in Indonesians. Methods: Twenty erectile dysfunction patients aged between 32-74 years old were recruited in this study. The inclusion criteria were as follows: 1) adult males 18 years or older with a subjective complaint or erectile dysfunction, 2) to provide written informed consent, 3) to agree not to use other forms of treatment for erectile dysfunction, 4) fulfill the screening laboratory values. Part 1, eligible patients were titrated in the clinic starting with a dose of 250 microg and proceed in a stepwise manner to 500 microg and 1,000 microg on separate clinic visits until they identified a dose that produced a satisfactory response. The interval between each in-clinic titration was 2-3 days. Each in-clinic titration dose was evaluated at 15 min intervals over a one hour period for erection assessment, blood pressure and pulse. Part 2, patients used MUSE at home for three months at the dose identified during the in-clinic titration. Monthly interim visits were required for patient follow-up and drug distribution. At the end of the study, patients had another laboratory (except testosterone, only assayed in screening procedure) and physical examination. Results: The etiology of erectile dysfunction was psychological in 5 patients and organic in 15 patients. The 65% of the patients achieved the erection scale of 4 or 5 either in the clinic or at home, 10% achieved the scale of 4 at home, but not in the clinic, and 25% only achieved the scale of 2 or 3 with the highest dose of 1,000 microg either in the clinic or at home. No significant differences were found in biochemical examination before and after the study. The 60% of the patients who achieved erection scale 4 or 5 continued to use MUSE until the end of the study, while 40% of them complained of pain at the time of MUSE application, during erection and/or during intercourse. They withdrew from the study. Conclusion: Transurethral application of alprostadil (MUSE) is effective and safe to produce erection sufficient for intercourse in erectile dysfunction of various etiologies. Pain during application, erection and intercourse is a common side effect and a cause of withdrawal.
International Journal of Impotence Research, Jun 16, 2011
The Asia-Pacific Sexual Health and Overall Wellness (AP SHOW) survey assessed sexual satisfaction... more The Asia-Pacific Sexual Health and Overall Wellness (AP SHOW) survey assessed sexual satisfaction and health and the association with erection hardness in 13 Asia-Pacific countries/ regions. Adults aged 25-74 years who had sexual intercourse X1 time in the past 12 months answered Internet surveys or participated in street-intercept interviews. Data were weighted by demographics and country/region size. Of 3957 (men, n ¼ 2016 and women, n ¼ 1941) respondents, 41% of men and 34% of women were completely or very satisfied with sex. Satisfaction with sex was linked to satisfaction with life priorities and overall health. Few respondents (men, 38% and women, 26%) were very or completely satisfied with erection hardness. Optimal erection hardness was reported by 45% of men (48% of women regarding their partners' erections). Erection hardness was associated with increased frequency of sex and importance of and satisfaction with erectionrelated elements of men's sexual performance. Approximately half of respondents (men, 57% and women, 47%) were at least moderately interested in improving the sexual experience. Most Asia-Pacific respondents were less than very satisfied with sex. Satisfaction with sex was associated with satisfaction with life priorities. Erection hardness was associated with sexual satisfaction and activity, satisfaction with life priorities and overall health.
IJAAM (Indonesian Journal of Anti Aging Medicine), Nov 5, 2019
Introduction: Diabetes mellitus is a complex and progressive disease which often lead to several ... more Introduction: Diabetes mellitus is a complex and progressive disease which often lead to several debilitating complications that partly caused by by free radicals which can be overcome with antioxidants. Bay leaves contain essential oils, tannins, flavonoids and terpenoids which have considerable antioxidant properties. Therefore, this study aims to determine the effect combination treatment of vildagliptin and bay leaf (Syzygium polyanthum) extract toward pancreatic beta cells density and glycated albumin levels in diabetic male Wistar rats (Rattus norvegicus). Methods: An experimental posttest only control group study was conducted using 36 albino male rats, aged 2-3 months, weighing 180-200 grams. The rats were divided into 2 groups (n= 18) with control group treated with 1.8mg/200g body weight vildagliptin and 2cc placebo (aquabidest) while the treatment group received 1.8mg/200g BW vildagliptin and 250 mg/200g BW bay leaves extract. All treatment lasted for 21 days. Results: The results showed that pancreatic beta cell counts in the treatment group was significantly higher than the control group (109.07 ± 20.47 cells/field of view vs 90.87 ± 13.91 cells/field of view; p<0.01). However, the levels of Glycated albumin between two groups were not significantly different (treatment vs control: 17.33 ± 4.51 vs 20.18 ± 4.57; p=0.068). Conclusion: This study suggested that combination treatment of vildagliptin and bay leaf extract increased pancreatic beta cells but did not reduce glycated albumin levels in diabetic male Wistar rats.
The relationship between the levels of adiponectin HMW and the metabolic syndrome component in menopausal women
Introduction: In menopausal women, there increasing evidence of adipocyte dysfunction which resul... more Introduction: In menopausal women, there increasing evidence of adipocyte dysfunction which result in a decrease in adiponectin HMW. The decrease in adiponectin HMW causes various pathological conditions related to aging such as metabolic syndrome. Simple adiponectin HMW measurements can evaluate early insulin resistance, metabolic disorders, or vascular health. The purpose of this study was to prove the relationship between adiponectin HMW levels and metabolic syndrome components in menopausal women. Methods: In this study, 45 postmenopausal women were used who declared physically healthy, met the criteria of metabolic syndrome based on IDF (International Diabetes Federation), willing to participate in the study and willing to fill out research forms as research subjects. This research was conducted at Panti Werdha Theodora in Makassar while the examination of HMW Adiponectin, fasting blood glucose, triglycerides, and High Density Lipoprotein was carried out in Prodia Laboratory. Result: The results showed a strong correlation between adiponectin HMW and metabolic syndrome components which included waist circumference, HDL levels, triglyceride levels, systolic blood pressure, diastolic and fasting blood glucose (p <0.05). Conclusion: It can be concluded that there was a relationship between adiponectin HMW levels and the metabolic syndrome component in menopausal women. HDL and waist circumference were the most significant factors compared to other metabolic factors associated with Adiponectin HMW.
International Journal of Andrology, Aug 1, 1991
The suppression of spermatogenesis by a combination of depot medroxyprogesterone acetate (DMPA) a... more The suppression of spermatogenesis by a combination of depot medroxyprogesterone acetate (DMPA) and testosterone enanthate (TE) was studied in Indonesian men. Twenty healthy, fertile volunteers were allocated randomly to either of two treatments each consisting of four intramuscular injections at monthly intervals. Group I ( n =10 men) received 100 mg DMPA plus 100 mg TE monthly while group I1 ( n = 10 men) received 200 mg DMPA plus 250 mg TE monthly. Sperm concentration was suppressed markedly, with all men attaining azoospermia between the third and fourth month after the start of treatment. There was no significant difference in the suppression of spermatogenesis between the two dosage regimens. The median time to reaching azoospermia was 2.5 months from the onset of injections and the median time to recovery of sperm in the ejaculate was 2.0 months after cessation of treatment. Both steroid regimens were equally effective in suppressing LH, FSH and testosterone levels. Testosterone levels returned to baseline by the fourth post-treatment month while LH and FSH demonstrated significant rebound above baseline levels from 3 to 5 months after cessation of treatment. No serious clinical side effects were observed. Weight gain and increases in libido were reported during treatment by most volunteers. A transient decrease in libido was noted in 5/20 (25%) men between 1-2 months after cessation of injections, presumably due to the prolonged effects of DMPA relative to TE. These results indicate that uniform induction of reversible azoospermia with minimal side effects can be achieved in a non-Causasian population. This study therefore supports the feasibility of an effective and reversible hormonal malecontraceptive in some populations even if this requires the consistent induction of azoospermia. The explanation for the higher frequency of azoospermia induced in Indonesian compared with Caucasian men remains to be clarified and is strategically critical to the future development of male contraception.
Introduction: Aging is a complex multifactorial process and leads to functional changes one of wh... more Introduction: Aging is a complex multifactorial process and leads to functional changes one of which is the decline in reproductive function. Decrease in reproductive function in women in in the form of decreased ovarian reserve can be detected by low levels of Anti_mullerian Hormone (AMH). This study aims to prove the effect of administration of Gallus domesticus boiled chicken white egg increased AMH levels of premenopausal female wistar rats. Methods: This research was experimental animal with Pretest-Posttest Control Group Design. The study conducted on 16 premenopausal female wistar mices, aged 17-18 months, weighing 250-260 grams. The mice were divided into 2 goups: the control group (placebo), which was given gelatin flour by 7.3 gram 3 times daily for 4 weeks and the group treted with Gallus domesticus boiled chicken white egg by 7.3 gram 3 times daily for 4 weeks by oral. Blood AMH level were measured before and after treatment for both group. Results: The result showed that before treatment, mean AMH level both in control group and treatment group were not significantly different (p > 0,05). After treatment, mean AMH level both in control group and treatment group were significantly different (p < 0,05). The administration of Gallus domesticus boiled chicken white egg increased AMH levels by 0.804 ng/ml. Conclusion: It can be concluded that administration of Gallus domesticus boiled chicken white egg increased AMH level. The mean AMH level after treatment had a significant difference between control group (placebo) and treatment group with p = 0.001.
Introduction: Seventy-five percent of menopausal women have menopausal syndrome, one of which is ... more Introduction: Seventy-five percent of menopausal women have menopausal syndrome, one of which is vaginal atrophy. DHEA is the only source of postmenopausal sex steroid hormones that provides estrogen and androgens according to cell needs to function normally. The aim of this study is to prove that DHEA supplementation orally can increase epithelium thickness, smooth muscle tissue, and the number of vaginal blood vessels of adult white mice (Rattus norvegicus) after bilateral ovarectomy. Method: This study used true experimental post-test only control group design using 36 white mice aged 10-12 weeks with a body weight of 180-200 grams after bilateral ovarectomy. The mice were divided into two groups with 18 mice each. The first group (controls) was given glycerin orally at a dose of 1 ml per day while the second group (treatment) was given 7.2 mg DHEA dissolved in 1 ml glycerin orally per day. On day 43, vaginal tissue was taken and histopathological examination was performed. Result: The results showed that the mean of epithelial thickness of treatment group was significantly higher than control group (51.397 µm vs. 29.755 µm; p: < 0.05). The smooth muscle thickness was also significantly higher in treatment group compared to control (74.136 µm vs. 47.428 µm; p: <0.05). Finally, the number of blood vessels was also significantly higher in treatment group (18.39 vs. 9.50; p:<0.05). Conclusion: It can be concluded that DHEA supplementation increases epithelium thickness, smooth muscle tissue thickness, and the number of vaginal blood vessels in adult rats after bilateral ovarectomy.
International Journal of Research Publication and Reviews, Jul 1, 2023
Introduction: The decrease of estrogen in the aging process causes various complaints in women. T... more Introduction: The decrease of estrogen in the aging process causes various complaints in women. This causes limitations in physical activity which ultimately reduces the quality of life. The use of phytopharmaca that has phytoestrogen activity is expected to help reduce the symptoms and complaints caused by a decrease in estrogen. This is due to its ability to bind to estrogen receptors. Basil leaves have phytoestrogen activity so that they can be an alternative in dealing with premenopausal symptoms. This study aims to prove that the ethanol extract of basil leaves can inhibit the decrease in the number of ovarian follicles and increase estradiol levels in premenopausal female Wistar rats. Methods: This research was an experimental post-test only control group design using female rats as the object of research, aged 10-18 months (human equivalent aged 40-45 years). The sample was divided into 2 groups, the treatment group which was given ethanol extract of basil leaf at a dosage of 0.50 g/kg BW and the control group which was given aquabidest every day orally for 21 days. After 21 days, blood was taken through the medial canthus of the orbital sinus to check estradiol levels using the ELISA method and the ovarian organs were taken for histological follicle counting. Results: The mean of follicles in the control group was 5.39 ± 1.14/LPB while the mean in the treatment group was 13.88 ± 2.87/LBP. These data indicate that the mean of the treatment group is higher than the control group. Statistical tests using the Mann Whitney test showed that there was a significant difference between the control and treatment groups (p=0.001). In examining estrogen levels, the mean of estrogen level for the control group was 207.02 ± 7.13 ng/L, while the treatment group was 229.56 ± 10.02 ng/L. Statistical tests using the Mann Whitney test showed that there was increased of estrogen level in the treatment groups (p=0.001). Conclusion: Oral administration of 0.5 g/kg BW ethanol extract of basil leaves (Ocimum basilicum L) inhibited the decrease of ovarian follicles number and increase estrogen levels in premenopausal female Wistar rats.
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Papers by Wimpie Pangkahila