Tacaribe virus (TACV) is an arenavirus that is genetically and antigenically closely related to J... more Tacaribe virus (TACV) is an arenavirus that is genetically and antigenically closely related to Junin virus (JUNV), the aetiological agent of Argentine haemorrhagic fever (AHF). It is well established that TACV protects experimental animals fully against an otherwise lethal challenge with JUNV. To gain information on the nature of the antigens involved in cross-protection, recombinant vaccinia viruses were constructed that express the glycoprotein precursor (VV–GTac) or the nucleocapsid protein (VV–N) of TACV. TACV proteins expressed by vaccinia virus were indistinguishable from authentic virus proteins by gel electrophoresis. Guinea pigs inoculated with VV–GTac or VV–N elicited antibodies that immunoprecipitated authentic TACV proteins. Antibodies generated by VV–GTac neutralized TACV infectivity. Levels of antibodies after priming and boosting with recombinant vaccinia virus were comparable to those elicited in TACV infection. To evaluate the ability of recombinant vaccinia virus ...
Hantavirus pulmonary syndrome (HPS) with high mortality rate has been reported in five countries ... more Hantavirus pulmonary syndrome (HPS) with high mortality rate has been reported in five countries in South America. Rapid accurate methods are important both for monitoring acute infections and for epidemiological studies. The Andes virus nucleoprotein amino acid sequence has a high identity percentage compared with other sequences of this region and has been chosen for the development of diagnostic reagents. Andes nucleoprotein expressed in Escherichia coli was applied as antigen in IgG, IgA and mu-capture IgM enzyme-linked inmunosorbent assays (ELISAs). An evaluation of this reagent was conducted to establish its usefulness for differential diagnosis of HPS and seroprevalence studies. Samples from 135 reverse transcription (RT)-PCR-confirmed HPS cases, 77 individuals with other respiratory infections and 957 healthy inhabitants from endemic and non-endemic areas were analysed. The hantavirus-infected patients had an early and strong IgM, IgG and IgA serum antibody response, in most...
An increase of Hantavirus Pulmonary Syndrome (HPS) cases around a southwestern Argentina town and... more An increase of Hantavirus Pulmonary Syndrome (HPS) cases around a southwestern Argentina town and in persons living 1400 km away but in contact with those cases was detected during the spring of 1996. In order to evaluate person-to-person transmission we compared the homology of PCR-amplified viral sequences of 26 Argentine and Chilean cases. Sixteen of them were epidemiologically linked cases and had the same sequence (Epilink/96) in the S segment 3Ј noncoding region and in the M segment partial G1 and G2 region (a total of 1075 nucleotides). Contrarily, two geographical and contemporary but nonepidemiologically related cases differed from Epilink/96 in the compared regions. No significant differences, such as glycosylation or hydrophilic pattern, were found between Epilink/96 and the other sequences. Nucleotide and deduced amino acid sequence homologies between samples from southern Argentina and Chile ranged from 90.9 to 100% and 96.4 to 100%, respectively. Phylogenetic analysis revealed that all the analyzed southwestern viruses belong to the Andes lineage. Although human infection principally occurs via inhalation of contaminated rodent excreta, our results with Andes virus show the first direct genetic evidence of person-to-person transmission of a hantavirus.
Las tecnologías de la información y las comunicaciones constituyen un campo muy activo para ofrec... more Las tecnologías de la información y las comunicaciones constituyen un campo muy activo para ofrecer un valor añadido al turista. Los destinos deben proporcionar herramientas tecnológicas capaces de ofrecer información personalizada, interactiva, etc. Actualmente es muy común el uso de herramientas como guías o páginas Web que cubren las necesidades básicas de los turistas. Se tratan de recursos insuficientes, pues la evolución de los dispositivos móviles ha provocado un cambio en la forma en que los turistas interactúan y obtienen información. Un caso concreto es la realidad aumentada, que permite desarrollar aplicaciones que enriquecen la experiencia turística. En este ámbito, resulta de especial interés la personalización de la información a través de sistemas de recomendación. Se presenta en este trabajo un enfoque de sistema de realidad aumentada aplicada al turismo basada en el contexto que utiliza técnicas de recomendación para visitas en grupo en un sistema integrado de gestión de destinos (SIGD). PALABRAS CLAVE: realidad aumentada, calidad turística, sistemas de recomendación, tecnología móvil, sistemas de gestión de destinos.
Se realizo un diseno multimetodo con el proposito de generar una aproximacion a la creatividad do... more Se realizo un diseno multimetodo con el proposito de generar una aproximacion a la creatividad docente en un contexto de masificacion estudiantil. En el momento cuantitativo se identificaron las variables utilizadas por los docentes: originalidad, flexibilidad, fluidez, divergencia, motivacion e innovacion; estableciendose un muestreo intencionado de seis sujetos, con base en el indice de respuestas positivas. Posteriormente en un momento cualitativo utilizando una entrevista apreciativa y observacion directa desde una postura hermeneutica-fenomenologica se exploro en detalle dichas variables. Emergieron categorias que generaron una aproximacion a un modelo teorico de creatividad docente; que en cuanto al accionar contempla: universalidad en las clases, identificacion del lider, trabajo compartido; y en aspectos a desarrollar: escuchar, usos de analogias-metaforas y libertad en clase. Las categorias y reflexiones generadas proporcionan al docente estrategias y herramientas para el espacio universitario masificado.
A fatal case of serologically confirmed hantavirus pulmonary syndrome (HPS) was recently reported... more A fatal case of serologically confirmed hantavirus pulmonary syndrome (HPS) was recently reported in southwestern Argentina. Nucleotide sequence analysis of PCR fragments from conserved regions of the S and M genomic segments of the virus, amplified from RNA extracted from autopsy lung and liver tissues, showed the virus (referred as Andes virus) to be novel. Comparisons between Andes virus genome sequences with the corresponding sequences of the more closely related hantaviruses revealed differences at the amino acid level from 13.6 to 23.9% for G2 protein regions and from 8.5 to 12.5% for the amino terminal region of the nucleocapsid protein. Phylogenetic analysis using the maximum parsimony and maximum likelihood methods showed that Andes virus maps within the clade containing the HPS-associated viruses from North America. Within this group, Andes virus represents a unique lineage. This is, to our knowledge, the first report on the genetic characterization of a hantavirus from South America.
Tacaribe virus (TACV) is an arenavirus that is genetically and antigenically closely related to J... more Tacaribe virus (TACV) is an arenavirus that is genetically and antigenically closely related to Junin virus (JUNV), the aetiological agent of Argentine haemorrhagic fever (AHF). It is well established that TACV protects experimental animals fully against an otherwise lethal challenge with JUNV. To gain information on the nature of the antigens involved in cross-protection, recombinant vaccinia viruses were constructed that express the glycoprotein precursor (VV–GTac) or the nucleocapsid protein (VV–N) of TACV. TACV proteins expressed by vaccinia virus were indistinguishable from authentic virus proteins by gel electrophoresis. Guinea pigs inoculated with VV–GTac or VV–N elicited antibodies that immunoprecipitated authentic TACV proteins. Antibodies generated by VV–GTac neutralized TACV infectivity. Levels of antibodies after priming and boosting with recombinant vaccinia virus were comparable to those elicited in TACV infection. To evaluate the ability of recombinant vaccinia virus ...
Hantavirus pulmonary syndrome (HPS) with high mortality rate has been reported in five countries ... more Hantavirus pulmonary syndrome (HPS) with high mortality rate has been reported in five countries in South America. Rapid accurate methods are important both for monitoring acute infections and for epidemiological studies. The Andes virus nucleoprotein amino acid sequence has a high identity percentage compared with other sequences of this region and has been chosen for the development of diagnostic reagents. Andes nucleoprotein expressed in Escherichia coli was applied as antigen in IgG, IgA and mu-capture IgM enzyme-linked inmunosorbent assays (ELISAs). An evaluation of this reagent was conducted to establish its usefulness for differential diagnosis of HPS and seroprevalence studies. Samples from 135 reverse transcription (RT)-PCR-confirmed HPS cases, 77 individuals with other respiratory infections and 957 healthy inhabitants from endemic and non-endemic areas were analysed. The hantavirus-infected patients had an early and strong IgM, IgG and IgA serum antibody response, in most...
An increase of Hantavirus Pulmonary Syndrome (HPS) cases around a southwestern Argentina town and... more An increase of Hantavirus Pulmonary Syndrome (HPS) cases around a southwestern Argentina town and in persons living 1400 km away but in contact with those cases was detected during the spring of 1996. In order to evaluate person-to-person transmission we compared the homology of PCR-amplified viral sequences of 26 Argentine and Chilean cases. Sixteen of them were epidemiologically linked cases and had the same sequence (Epilink/96) in the S segment 3Ј noncoding region and in the M segment partial G1 and G2 region (a total of 1075 nucleotides). Contrarily, two geographical and contemporary but nonepidemiologically related cases differed from Epilink/96 in the compared regions. No significant differences, such as glycosylation or hydrophilic pattern, were found between Epilink/96 and the other sequences. Nucleotide and deduced amino acid sequence homologies between samples from southern Argentina and Chile ranged from 90.9 to 100% and 96.4 to 100%, respectively. Phylogenetic analysis revealed that all the analyzed southwestern viruses belong to the Andes lineage. Although human infection principally occurs via inhalation of contaminated rodent excreta, our results with Andes virus show the first direct genetic evidence of person-to-person transmission of a hantavirus.
Las tecnologías de la información y las comunicaciones constituyen un campo muy activo para ofrec... more Las tecnologías de la información y las comunicaciones constituyen un campo muy activo para ofrecer un valor añadido al turista. Los destinos deben proporcionar herramientas tecnológicas capaces de ofrecer información personalizada, interactiva, etc. Actualmente es muy común el uso de herramientas como guías o páginas Web que cubren las necesidades básicas de los turistas. Se tratan de recursos insuficientes, pues la evolución de los dispositivos móviles ha provocado un cambio en la forma en que los turistas interactúan y obtienen información. Un caso concreto es la realidad aumentada, que permite desarrollar aplicaciones que enriquecen la experiencia turística. En este ámbito, resulta de especial interés la personalización de la información a través de sistemas de recomendación. Se presenta en este trabajo un enfoque de sistema de realidad aumentada aplicada al turismo basada en el contexto que utiliza técnicas de recomendación para visitas en grupo en un sistema integrado de gestión de destinos (SIGD). PALABRAS CLAVE: realidad aumentada, calidad turística, sistemas de recomendación, tecnología móvil, sistemas de gestión de destinos.
Se realizo un diseno multimetodo con el proposito de generar una aproximacion a la creatividad do... more Se realizo un diseno multimetodo con el proposito de generar una aproximacion a la creatividad docente en un contexto de masificacion estudiantil. En el momento cuantitativo se identificaron las variables utilizadas por los docentes: originalidad, flexibilidad, fluidez, divergencia, motivacion e innovacion; estableciendose un muestreo intencionado de seis sujetos, con base en el indice de respuestas positivas. Posteriormente en un momento cualitativo utilizando una entrevista apreciativa y observacion directa desde una postura hermeneutica-fenomenologica se exploro en detalle dichas variables. Emergieron categorias que generaron una aproximacion a un modelo teorico de creatividad docente; que en cuanto al accionar contempla: universalidad en las clases, identificacion del lider, trabajo compartido; y en aspectos a desarrollar: escuchar, usos de analogias-metaforas y libertad en clase. Las categorias y reflexiones generadas proporcionan al docente estrategias y herramientas para el espacio universitario masificado.
A fatal case of serologically confirmed hantavirus pulmonary syndrome (HPS) was recently reported... more A fatal case of serologically confirmed hantavirus pulmonary syndrome (HPS) was recently reported in southwestern Argentina. Nucleotide sequence analysis of PCR fragments from conserved regions of the S and M genomic segments of the virus, amplified from RNA extracted from autopsy lung and liver tissues, showed the virus (referred as Andes virus) to be novel. Comparisons between Andes virus genome sequences with the corresponding sequences of the more closely related hantaviruses revealed differences at the amino acid level from 13.6 to 23.9% for G2 protein regions and from 8.5 to 12.5% for the amino terminal region of the nucleocapsid protein. Phylogenetic analysis using the maximum parsimony and maximum likelihood methods showed that Andes virus maps within the clade containing the HPS-associated viruses from North America. Within this group, Andes virus represents a unique lineage. This is, to our knowledge, the first report on the genetic characterization of a hantavirus from South America.
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