Biomedical Engineering Society Annual meeting, 2013
Introduction: Microfluidic cell sorting using dielectrophoresis (DEP) has emerged as a popular to... more Introduction: Microfluidic cell sorting using dielectrophoresis (DEP) has emerged as a popular tool to isolate cell subpopulations in engineering research laboratories. However, adoption of this technique by biologists and medical scientists has been hindered by significant issues, including the requirement for complex fabrication or instrumentation schemes as well as insufficient numbers of isolated cells for studies such as cell transplantation. Using Large Capacity Electrode Arrays (LCEAs), here we demonstrate a technique to enable isolation of larger numbers of cell subpopulations of interest using DEP. In addition, the system requires no pumps, valves, or complex circuitry, enabling adoption of the technique by scientists with little experience in microfabrication or electrical engineering concepts.
Using matching and regression analyses, we measure the difference in citations between articles p... more Using matching and regression analyses, we measure the difference in citations between articles posted to Academia.edu and other articles from similar journals, controlling for field, impact factor, and other variables. Based on a sample size of 31,216 papers, we find that a paper in a median impact factor journal uploaded to Academia.edu receives 16% more citations after one year than a similar article not available online, 51% more citations after three years, and 69% after five years. We also found that articles also posted to Academia.edu had 58% more citations than articles only posted to other online venues, such as personal and departmental home pages, after five years.
Dielectrophoresis (DEP) has proven an invaluable tool for the enrichment of populations of stem a... more Dielectrophoresis (DEP) has proven an invaluable tool for the enrichment of populations of stem and progenitor cells owing to its ability to sort cells in a label- free manner and its biological safety. However, DEP separation devices have suf- fered from a low throughput preventing researchers from undertaking studies requiring large numbers of cells, such as needed for cell transplantation. We devel- oped a microfluidic device designed for the enrichment of stem and progenitor cell populations that sorts cells at a rate of 150,000 cells/h, corresponding to an improvement in the throughput achieved with our previous device designs by over an order of magnitude. This advancement, coupled with data showing the DEP- sorted cells retain their enrichment and differentiation capacity when expanded in culture for periods of up to 2 weeks, provides sufficient throughput and cell num- bers to enable a wider variety of experiments with enriched stem and progenitor cell populations. Furthermore, the sorting devices presented here provide ease of setup and operation, a simple fabrication process, and a low associated cost to use that makes them more amenable for use in common biological research laborato- ries. To our knowledge, this work represents the first to enrich stem cells and expand them in culture to generate transplantation-scale numbers of differentiation-competent cells using DEP. VC 2014 AIP Publishing LLC. [http://dx.doi.org/10.1063/1.4902371]
Introduction: Microfluidic cell sorting using dielectrophoresis (DEP) has emerged as a popular to... more Introduction: Microfluidic cell sorting using dielectrophoresis (DEP) has emerged as a popular tool to isolate cell subpopulations in engineering research laboratories. However, adoption of this technique by biologists and medical scientists has been hindered by significant issues, including the requirement for complex fabrication or instrumentation schemes as well as insufficient numbers of isolated cells for studies such as cell transplantation. Using Large Capacity Electrode Arrays (LCEAs), here we demonstrate a technique to enable isolation of larger numbers of cell subpopulations of interest using DEP. In addition, the system requires no pumps, valves, or complex circuitry, enabling adoption of the technique by scientists with little experience in microfabrication or electrical engineering concepts.
Biomedical Engineering Society Annual meeting, 2013
Introduction: Microfluidic cell sorting using dielectrophoresis (DEP) has emerged as a popular to... more Introduction: Microfluidic cell sorting using dielectrophoresis (DEP) has emerged as a popular tool to isolate cell subpopulations in engineering research laboratories. However, adoption of this technique by biologists and medical scientists has been hindered by significant issues, including the requirement for complex fabrication or instrumentation schemes as well as insufficient numbers of isolated cells for studies such as cell transplantation. Using Large Capacity Electrode Arrays (LCEAs), here we demonstrate a technique to enable isolation of larger numbers of cell subpopulations of interest using DEP. In addition, the system requires no pumps, valves, or complex circuitry, enabling adoption of the technique by scientists with little experience in microfabrication or electrical engineering concepts.
Using matching and regression analyses, we measure the difference in citations between articles p... more Using matching and regression analyses, we measure the difference in citations between articles posted to Academia.edu and other articles from similar journals, controlling for field, impact factor, and other variables. Based on a sample size of 31,216 papers, we find that a paper in a median impact factor journal uploaded to Academia.edu receives 16% more citations after one year than a similar article not available online, 51% more citations after three years, and 69% after five years. We also found that articles also posted to Academia.edu had 58% more citations than articles only posted to other online venues, such as personal and departmental home pages, after five years.
Dielectrophoresis (DEP) has proven an invaluable tool for the enrichment of populations of stem a... more Dielectrophoresis (DEP) has proven an invaluable tool for the enrichment of populations of stem and progenitor cells owing to its ability to sort cells in a label- free manner and its biological safety. However, DEP separation devices have suf- fered from a low throughput preventing researchers from undertaking studies requiring large numbers of cells, such as needed for cell transplantation. We devel- oped a microfluidic device designed for the enrichment of stem and progenitor cell populations that sorts cells at a rate of 150,000 cells/h, corresponding to an improvement in the throughput achieved with our previous device designs by over an order of magnitude. This advancement, coupled with data showing the DEP- sorted cells retain their enrichment and differentiation capacity when expanded in culture for periods of up to 2 weeks, provides sufficient throughput and cell num- bers to enable a wider variety of experiments with enriched stem and progenitor cell populations. Furthermore, the sorting devices presented here provide ease of setup and operation, a simple fabrication process, and a low associated cost to use that makes them more amenable for use in common biological research laborato- ries. To our knowledge, this work represents the first to enrich stem cells and expand them in culture to generate transplantation-scale numbers of differentiation-competent cells using DEP. VC 2014 AIP Publishing LLC. [http://dx.doi.org/10.1063/1.4902371]
Introduction: Microfluidic cell sorting using dielectrophoresis (DEP) has emerged as a popular to... more Introduction: Microfluidic cell sorting using dielectrophoresis (DEP) has emerged as a popular tool to isolate cell subpopulations in engineering research laboratories. However, adoption of this technique by biologists and medical scientists has been hindered by significant issues, including the requirement for complex fabrication or instrumentation schemes as well as insufficient numbers of isolated cells for studies such as cell transplantation. Using Large Capacity Electrode Arrays (LCEAs), here we demonstrate a technique to enable isolation of larger numbers of cell subpopulations of interest using DEP. In addition, the system requires no pumps, valves, or complex circuitry, enabling adoption of the technique by scientists with little experience in microfabrication or electrical engineering concepts.
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Papers by Adnan Akil
This is a revised version that has been accepted for publication in PLOS ONE. The updated paper, dataset, and code, as well as previous versions of each, are available on the Github repo: https://github.com/polynumeral/academia-citations
This is a revised version that has been accepted for publication in PLOS ONE. The updated paper, dataset, and code, as well as previous versions of each, are available on the Github repo: https://github.com/polynumeral/academia-citations