Tomographic imaging has been a widely used tool in medicine as it can provide three-dimensional (... more Tomographic imaging has been a widely used tool in medicine as it can provide three-dimensional (3D) structural information regarding objects of different size scales. In micrometer and millimeter scales, optical microscopy modalities find increasing use owing to the non-ionizing nature of visible light, and the availability of a rich set of illumination sources (such as lasers and light-emitting-diodes) and detection elements (such as large format CCD and CMOS detector-arrays).
Abstract Improving the access to effective and affordable healthcare has long been a global endea... more Abstract Improving the access to effective and affordable healthcare has long been a global endeavor. In this quest, the development of cost-effective and easy-to-use medical testing equipment that enable rapid and accurate diagnosis is essential to reduce the time and 10 costs associated with healthcare services. To this end, point-of-care (POC) diagnostics plays a crucial role in healthcare delivery in both the developed and developing countries by bringing medical testing to patients, or to sites near patients.
Abstract Lensfree on-chip holographic microscopy is an emerging technique that offers imaging of ... more Abstract Lensfree on-chip holographic microscopy is an emerging technique that offers imaging of biological specimens over a large field-of-view without using any lenses or bulky optical components. Lending itself to a compact, cost-effective, and mechanically robust architecture, lensfree on-chip holographic microscopy can offer an alternative toolset addressing some of the emerging needs of microscopic analysis and diagnostics in low-resource settings, especially for telemedicine applications.
Abstract: A system for three dimensional imaging of an object contained within a sample includes ... more Abstract: A system for three dimensional imaging of an object contained within a sample includes an image sensor, a sample holder configured to hold the sample, the sample holder disposed adjacent to the image sensor, and an illumination source comprising partially coherent light.
Abstract The first demonstration of optofluidic tomography is presented. Using partially coherent... more Abstract The first demonstration of optofluidic tomography is presented. Using partially coherent illumination, holograms of objects are recorded at multiple viewing angles, as they flow through a microfluidic channel placed directly on the top of an opto-electronic sensor array. These lensfree holograms are then digitally processed to compute pixel super-resolved tomograms of micro-objects to achieve sectional opto-fluidic imaging on a chip.
Computation is becoming an indispensible part of most microscopy systems. In particular, three-di... more Computation is becoming an indispensible part of most microscopy systems. In particular, three-dimensional (3D) imaging techniques heavily depend on efficient use of computation for both image reconstruction and visualization [1]-[5]. Similarly, recently emerging lensfree holographic microscopy techniques also rely on the use of digital processing to replace bulky optical components, such as objective lenses, found in conventional light microscopes, and achieve high-throughput microscopic imaging on a chip [6]-[10].
Abstract We demonstrate a telemedicine microscopy platform which operates based on lens free digi... more Abstract We demonstrate a telemedicine microscopy platform which operates based on lens free digital holography. It utilizes an incoherent light-source and an opto-electronic sensor-array to record lens free holograms of micro-objects within a sample, which are then rapidly processed using custom-developed algorithms to provide microscopic images of the samples without the use of any lenses, lasers or other bulky optical/mechanical components.
The recent revolution in digital technologies and information processing methods present importan... more The recent revolution in digital technologies and information processing methods present important opportunities to transform the way optical imaging is performed, particularly toward improving the throughput of microscopes while at the same time reducing their relative cost and complexity.
Abstract: A system for imaging a cytological sample includes a sample holder configured to hold a... more Abstract: A system for imaging a cytological sample includes a sample holder configured to hold a cytological sample. A spatial filter is disposed at a distance z 1 from the sample holder on first side of the sample holder, the spatial filter having an aperture disposed therein configured to allow the passage of illumination. An imaging sensor array is disposed at a distance z 2 from the sample holder on a second, opposite side of the sample holder.
An imaging device includes a scanning platform coupled to a fixed platform by flexible members. T... more An imaging device includes a scanning platform coupled to a fixed platform by flexible members. The scanning platform, fixed platform, and flexible members are made of a polymer such as is commonly used for printed circuit boards. The scanning platform has a laser light source, focusing lens, variable focus mechanism, photodetector, and light collection optic mounted thereto. The variable focus mechanism can sweep the outgoing laser light focus to determine the reflection distance.
Abstract Microfluidic devices aim at miniaturizing, automating, and lowering the cost of chemical... more Abstract Microfluidic devices aim at miniaturizing, automating, and lowering the cost of chemical and biological sample manipulation and detection, hence creating new opportunities for lab-on-a-chip platforms. Recently, optofluidic devices have also emerged where optics is used to enhance the functionality and the performance of microfluidic components in general.
Abstract FR4 is a well-engineered material and widely used in the PCB industry and lend itself to... more Abstract FR4 is a well-engineered material and widely used in the PCB industry and lend itself to high degree of integration of optoelectronic, micro-optic, and electronic devices. FR4 is used for the first time as an actuated mechanical device that integrates several functions on the same device. Two different approaches to 2D laser scanning using a single electromagnetic actuation coil and application to Fourier Transform spectroscopy are presented; many other applications can be envisioned.
A barcode scanner includes a scanning platform coupled to a fixed platform by flexible members. T... more A barcode scanner includes a scanning platform coupled to a fixed platform by flexible members. The scanning platform, fixed platform, and flexible members are made of a polymer such as is commonly used for printed circuit boards. The scanning platform has a laser light source, focusing lens, photodetector, and light collection optic mounted thereto. The polymer qualities and the moment of inertia of the scanning platform can be controlled to achieve a desired mechanical resonance.
Abstract The use of optical imaging for medical diagnostics at the point of care (POC) has great ... more Abstract The use of optical imaging for medical diagnostics at the point of care (POC) has great potential, but is limited by cost and the need for highly trained personnel. To this end, the cost, complexity, and size of optical microscopy devices can be reduced through the use of computation. These techniques can perform particularly well at specific tasks such as cytometry, water quality management, and disease diagnostics.
Abstract We demonstrate an on-chip polarization microscope based on incoherent lensfree holograph... more Abstract We demonstrate an on-chip polarization microscope based on incoherent lensfree holography. Placed between two polarizers, a birefringent sample creates in-line holograms that can be digitally reconstructed to create lensfree polarization images over a large field-of-view.
We present a field-portable lensfree tomographic microscope, which can achieve sectional imaging ... more We present a field-portable lensfree tomographic microscope, which can achieve sectional imaging of a large volume ($20 mm3) on a chip with an axial resolution of< 7 μm.
We demonstrate a field-portable upright and inverted microscope that can image specimens in both ... more We demonstrate a field-portable upright and inverted microscope that can image specimens in both reflection and transmission modes. This compact and cost-effective dual-mode microscope weighs only $135 grams (< 4.8 ounces) and utilizes a simple light emitting diode (LED) to illuminate the sample of interest using a beam-splitter cube that is positioned above the object plane.
Three-dimensional microscopy techniques have found increasing use in the life sciences because th... more Three-dimensional microscopy techniques have found increasing use in the life sciences because they provide high-resolution structural information of biological specimens. 1 Numerous approaches to achieve 3D optical imaging have been demonstrated, for example, optical coherence tomography, confocal microscopy, diffraction tomography, 2 optical projection tomography, 3 and light-sheet microscopy.
ABSTRACT We present a lensfree digital microscopy platform implemented on a cell-phone. It operat... more ABSTRACT We present a lensfree digital microscopy platform implemented on a cell-phone. It operates based on digital in-line holography and provides a compact and light-weight alternative to conventional microscopes, such that the cell-phone is modified with an inexpensive attachment weighing only~ 38 grams. This lensfree cell-phone microscope captures holographic images of the objects which are then rapidly processed by a custom-developed reconstruction algorithm to provide microscopic images of the sample.
Tomographic imaging has been a widely used tool in medicine as it can provide three-dimensional (... more Tomographic imaging has been a widely used tool in medicine as it can provide three-dimensional (3D) structural information regarding objects of different size scales. In micrometer and millimeter scales, optical microscopy modalities find increasing use owing to the non-ionizing nature of visible light, and the availability of a rich set of illumination sources (such as lasers and light-emitting-diodes) and detection elements (such as large format CCD and CMOS detector-arrays).
Abstract Improving the access to effective and affordable healthcare has long been a global endea... more Abstract Improving the access to effective and affordable healthcare has long been a global endeavor. In this quest, the development of cost-effective and easy-to-use medical testing equipment that enable rapid and accurate diagnosis is essential to reduce the time and 10 costs associated with healthcare services. To this end, point-of-care (POC) diagnostics plays a crucial role in healthcare delivery in both the developed and developing countries by bringing medical testing to patients, or to sites near patients.
Abstract Lensfree on-chip holographic microscopy is an emerging technique that offers imaging of ... more Abstract Lensfree on-chip holographic microscopy is an emerging technique that offers imaging of biological specimens over a large field-of-view without using any lenses or bulky optical components. Lending itself to a compact, cost-effective, and mechanically robust architecture, lensfree on-chip holographic microscopy can offer an alternative toolset addressing some of the emerging needs of microscopic analysis and diagnostics in low-resource settings, especially for telemedicine applications.
Abstract: A system for three dimensional imaging of an object contained within a sample includes ... more Abstract: A system for three dimensional imaging of an object contained within a sample includes an image sensor, a sample holder configured to hold the sample, the sample holder disposed adjacent to the image sensor, and an illumination source comprising partially coherent light.
Abstract The first demonstration of optofluidic tomography is presented. Using partially coherent... more Abstract The first demonstration of optofluidic tomography is presented. Using partially coherent illumination, holograms of objects are recorded at multiple viewing angles, as they flow through a microfluidic channel placed directly on the top of an opto-electronic sensor array. These lensfree holograms are then digitally processed to compute pixel super-resolved tomograms of micro-objects to achieve sectional opto-fluidic imaging on a chip.
Computation is becoming an indispensible part of most microscopy systems. In particular, three-di... more Computation is becoming an indispensible part of most microscopy systems. In particular, three-dimensional (3D) imaging techniques heavily depend on efficient use of computation for both image reconstruction and visualization [1]-[5]. Similarly, recently emerging lensfree holographic microscopy techniques also rely on the use of digital processing to replace bulky optical components, such as objective lenses, found in conventional light microscopes, and achieve high-throughput microscopic imaging on a chip [6]-[10].
Abstract We demonstrate a telemedicine microscopy platform which operates based on lens free digi... more Abstract We demonstrate a telemedicine microscopy platform which operates based on lens free digital holography. It utilizes an incoherent light-source and an opto-electronic sensor-array to record lens free holograms of micro-objects within a sample, which are then rapidly processed using custom-developed algorithms to provide microscopic images of the samples without the use of any lenses, lasers or other bulky optical/mechanical components.
The recent revolution in digital technologies and information processing methods present importan... more The recent revolution in digital technologies and information processing methods present important opportunities to transform the way optical imaging is performed, particularly toward improving the throughput of microscopes while at the same time reducing their relative cost and complexity.
Abstract: A system for imaging a cytological sample includes a sample holder configured to hold a... more Abstract: A system for imaging a cytological sample includes a sample holder configured to hold a cytological sample. A spatial filter is disposed at a distance z 1 from the sample holder on first side of the sample holder, the spatial filter having an aperture disposed therein configured to allow the passage of illumination. An imaging sensor array is disposed at a distance z 2 from the sample holder on a second, opposite side of the sample holder.
An imaging device includes a scanning platform coupled to a fixed platform by flexible members. T... more An imaging device includes a scanning platform coupled to a fixed platform by flexible members. The scanning platform, fixed platform, and flexible members are made of a polymer such as is commonly used for printed circuit boards. The scanning platform has a laser light source, focusing lens, variable focus mechanism, photodetector, and light collection optic mounted thereto. The variable focus mechanism can sweep the outgoing laser light focus to determine the reflection distance.
Abstract Microfluidic devices aim at miniaturizing, automating, and lowering the cost of chemical... more Abstract Microfluidic devices aim at miniaturizing, automating, and lowering the cost of chemical and biological sample manipulation and detection, hence creating new opportunities for lab-on-a-chip platforms. Recently, optofluidic devices have also emerged where optics is used to enhance the functionality and the performance of microfluidic components in general.
Abstract FR4 is a well-engineered material and widely used in the PCB industry and lend itself to... more Abstract FR4 is a well-engineered material and widely used in the PCB industry and lend itself to high degree of integration of optoelectronic, micro-optic, and electronic devices. FR4 is used for the first time as an actuated mechanical device that integrates several functions on the same device. Two different approaches to 2D laser scanning using a single electromagnetic actuation coil and application to Fourier Transform spectroscopy are presented; many other applications can be envisioned.
A barcode scanner includes a scanning platform coupled to a fixed platform by flexible members. T... more A barcode scanner includes a scanning platform coupled to a fixed platform by flexible members. The scanning platform, fixed platform, and flexible members are made of a polymer such as is commonly used for printed circuit boards. The scanning platform has a laser light source, focusing lens, photodetector, and light collection optic mounted thereto. The polymer qualities and the moment of inertia of the scanning platform can be controlled to achieve a desired mechanical resonance.
Abstract The use of optical imaging for medical diagnostics at the point of care (POC) has great ... more Abstract The use of optical imaging for medical diagnostics at the point of care (POC) has great potential, but is limited by cost and the need for highly trained personnel. To this end, the cost, complexity, and size of optical microscopy devices can be reduced through the use of computation. These techniques can perform particularly well at specific tasks such as cytometry, water quality management, and disease diagnostics.
Abstract We demonstrate an on-chip polarization microscope based on incoherent lensfree holograph... more Abstract We demonstrate an on-chip polarization microscope based on incoherent lensfree holography. Placed between two polarizers, a birefringent sample creates in-line holograms that can be digitally reconstructed to create lensfree polarization images over a large field-of-view.
We present a field-portable lensfree tomographic microscope, which can achieve sectional imaging ... more We present a field-portable lensfree tomographic microscope, which can achieve sectional imaging of a large volume ($20 mm3) on a chip with an axial resolution of< 7 μm.
We demonstrate a field-portable upright and inverted microscope that can image specimens in both ... more We demonstrate a field-portable upright and inverted microscope that can image specimens in both reflection and transmission modes. This compact and cost-effective dual-mode microscope weighs only $135 grams (< 4.8 ounces) and utilizes a simple light emitting diode (LED) to illuminate the sample of interest using a beam-splitter cube that is positioned above the object plane.
Three-dimensional microscopy techniques have found increasing use in the life sciences because th... more Three-dimensional microscopy techniques have found increasing use in the life sciences because they provide high-resolution structural information of biological specimens. 1 Numerous approaches to achieve 3D optical imaging have been demonstrated, for example, optical coherence tomography, confocal microscopy, diffraction tomography, 2 optical projection tomography, 3 and light-sheet microscopy.
ABSTRACT We present a lensfree digital microscopy platform implemented on a cell-phone. It operat... more ABSTRACT We present a lensfree digital microscopy platform implemented on a cell-phone. It operates based on digital in-line holography and provides a compact and light-weight alternative to conventional microscopes, such that the cell-phone is modified with an inexpensive attachment weighing only~ 38 grams. This lensfree cell-phone microscope captures holographic images of the objects which are then rapidly processed by a custom-developed reconstruction algorithm to provide microscopic images of the sample.
Lensfree imaging is utilized to perform cytometry on a chip (http://innovate.ee.ucla.edu)
This work constitutes the first demonstration of lensfree optical tomography, which is an optical... more This work constitutes the first demonstration of lensfree optical tomography, which is an optical analog of X-Ray CT, implemented in the visible part of the electromagnetic spectrum.
We also demonstrate a field-portable tomographic microscope, as well as an optofluidic tomographic microscope for on-chip imaging applications.
Printed Circuits Boards (PCBs) are presented for the first time as actuated opto-mechatronic plat... more Printed Circuits Boards (PCBs) are presented for the first time as actuated opto-mechatronic platforms. The high degree of integration offered by scanning systems made of PCBs (main material: FR4 polymer) enables integrating lenses, lasers, circuits, ICs, actuation magnets and coils all on the same platform for highly miniaturized, compact optical devices, especially for bar-code readers and laser projection displays.
Uploads
Papers by Serhan Isikman
We also demonstrate a field-portable tomographic microscope, as well as an optofluidic tomographic microscope for on-chip imaging applications.