The aim of this work was to evaluate the potential of grape stalks, an agroindustrial waste, for ... more The aim of this work was to evaluate the potential of grape stalks, an agroindustrial waste, for growth and lignocellulolytic enzyme production via solid-state fermentation, using the following three white rot fungi: Trametes trogii, Stereum hirsutum and Coriolus antarcticus. The decolorization of several dyes by the above mentioned cultures was also investigated. Similar values of dry weight loss of the substrate were measured after 60 days (33-43 %). C. antarcticus produced the highest laccase and Mn-peroxldase activities (33.0 and 1.6 U/g dry solid). The maximum endoglucanase production was measured in S. hirsutum cultures (10.4 U/g), while the endoxylanase peak corresponded to T. trogii (14.6 U/g). The C. antarcticus/grape stalk system seems potentially competitive in bioremediation of textile processing effluents, attaining percentages of decolorization of 93, 86, 82, 82, 77, and 58 % for indigo carmine, malachite green, azure B, remazol brilliant blue R, crystal violet and xyl...
En Argentina existen actualmente bases de datos de aves, mamíferos y plantas entre otros, pero ex... more En Argentina existen actualmente bases de datos de aves, mamíferos y plantas entre otros, pero existe un vacío en lo que respecta a la diversidad fúngica. Esto puede deberse a la limitada cultura micofaga en el país. En otros países como España, Finlandia, Noruega y Suecia, existen sitios online que albergan descripciones, imágenes, mapas de distribución y listados con especies en peligro de extinción, satisfaciendo las necesidades tanto de la comunidad científica como también de aquellos que simplemente tienen interés por la flora fúngica. Dada la inmensa variedad de ambientes y escenarios que reúne la Argentina, es necesaria una base de datos online que reúna información de los hongos nativos, es por esto que en 2011 se comenzó el proyecto hongosdeargentina.com.ar con el objetivo de reunir imágenes de hongos encontradas en la Argentina, descripciones relevantes de las especies, comentarios de especialistas, región de distribución geográfica, importancia culinaria y tóxica, entre o...
The aim of this work was to evaluate the potential of grape stalks, an agroindustrial waste, for ... more The aim of this work was to evaluate the potential of grape stalks, an agroindustrial waste, for growth and lignocellulolytic enzyme production via solid-state fermentation, using the following three white rot fungi: Trametes trogii, Stereum hirsutum and Coriolus antarcticus. The decolorization of several dyes by the above mentioned cultures was also investigated. Similar values of dry weight loss of the substrate were measured after 60 days (33-43 %). C. antarcticus produced the highest laccase and Mn-peroxidase activities (33.0 and 1.6 U/g dry solid). The maximum endoglucanase production was measured in S. hirsutum cultures (10.4 U/g), while the endoxylanase peak corresponded to T. trogii (14.6 U/g). The C. antarcticus/grape stalk system seems potentially competitive in bioremediation of textile processing effluents, attaining percentages of decolorization of 93, 86, 82, 82, 77, and 58% for indigo carmine, malachite green, azure B, remazol brilliant blue R, crystal violet and xyli...
International Biodeterioration & Biodegradation, 2011
Trametes trogii BAFC 463 culture fluids (containing 110 U ml À1 laccase; 0.94 U ml À1 manganese p... more Trametes trogii BAFC 463 culture fluids (containing 110 U ml À1 laccase; 0.94 U ml À1 manganese peroxidase), as well as its purified laccase were capable of decolorizing azoic, indigoid, triphenylmethane, anthraquinonic and heterocyclic dyes, in the absence of redox mediators. Six dyes: RBBR, Indigo Carmine, Xylidine, Malachite Green, Gentian Violet and Bromophenol Blue were almost completely degraded (more than 85% decolorization after 1 d) by either laccase or T. trogii itself in culture, proving the role of the enzyme in dye decolorization. The purified laccase also decolorized 65% of Fast Blue RR and 30% of Azure B and Methylene Blue after 24 h. The use of redox mediators significantly increased the decolorization rates (90% decolorization of Azure B after 1 h). 1-hydroxybenzotriazole resulted the best redox mediator, but the natural mediator p-hydroxybenzoic acid also demonstrated its efficiency for dye decolorization. Due to their ability to decolorize recalcitrant dyes without addition of redox mediators, high laccase activities, high thermostability and efficient decolorization at 70 C and pH 7.0, even in the presence of high concentrations of heavy metals (100 mM Cu þ2 , Pb þ2 or Cd þ2 ) or in a synthetic dyebath, T. trogii culture fluids could be effectively used to decolorize synthetic dyes from effluents.
International Biodeterioration & Biodegradation, 2012
Culture filtrates from the white rot fungus Trametes trogii were used for the degradation of two ... more Culture filtrates from the white rot fungus Trametes trogii were used for the degradation of two azo dyes: Xylidine and Methyl Orange. These culture filtrates showed a great potential for decolorizing even high concentrations of both azo dyes, without the addition of redox mediators, possibly due to the synergistic action of their high contents of laccase (104 U ml À1 ), accompanied by Mn-peroxidase (0.54 U ml À1 ), cellobiose dehydrogenase (0.45 U ml À1 ) and glioxal oxidase (0.38 U ml À1 ) activities. 75% of Methyl Orange (980 ppm) and 96% of Xylidine (480 ppm) were degraded after 24 h, degradation occurred after 6 days with a 94% of Xylidine removed (1440 ppm). Biodegradation products from Xylidine were determined by capillary electrophoresis followed by MALDI-TOF mass spectra. Neither aromatic amines nor colored quinones were detected, major metabolites being naphtalene sulphonate and xylene.
La mejor forma de entender lo que tenemos y aprovecharlo, es conociendo lo que nos rodea, estudia... more La mejor forma de entender lo que tenemos y aprovecharlo, es conociendo lo que nos rodea, estudiarlo y aplicarlo. Bajo estas premisas, los hongos son un eslabón muy importante de la biodiversidad, que en este número nos impactan no sólo con sus formas y colores sino también desde el punto de vista de su aplicación en procesos productivos. El estudio de enzimas que producen los hongos para poder llevar adelante procesos productivos que se vinculan directamente con el desarrollo sustentable aprovechando los recursos biológicos de forma inteligente, nos muestra cómo es posible ir más allá del monte aprovechando una de sus infinitas herramientas escondida en la biodiversidad. El camino a seguir para lograr un aprovechamiento de los recursos existentes en nuestros bosques, con el agregado de valor de nuestros investigadores está marcado. Los aportes de la Dra. Laura Villalba y del Lic. Emanuel Grassi sobre sus experiencias en el CIAR y en el desarrollo de biotecnologías en base a estos o...
The aim of this work was to evaluate the potential of grape stalks, an agroindustrial waste, for ... more The aim of this work was to evaluate the potential of grape stalks, an agroindustrial waste, for growth and lignocellulolytic enzyme production via solid-state fermentation, using the following three white rot fungi: Trametes trogii, Stereum hirsutum and Coriolus antarcticus. The decolorization of several dyes by the above mentioned cultures was also investigated. Similar values of dry weight loss of the substrate were measured after 60 days (33-43 %). C. antarcticus produced the highest laccase and Mn-peroxldase activities (33.0 and 1.6 U/g dry solid). The maximum endoglucanase production was measured in S. hirsutum cultures (10.4 U/g), while the endoxylanase peak corresponded to T. trogii (14.6 U/g). The C. antarcticus/grape stalk system seems potentially competitive in bioremediation of textile processing effluents, attaining percentages of decolorization of 93, 86, 82, 82, 77, and 58 % for indigo carmine, malachite green, azure B, remazol brilliant blue R, crystal violet and xyl...
En Argentina existen actualmente bases de datos de aves, mamíferos y plantas entre otros, pero ex... more En Argentina existen actualmente bases de datos de aves, mamíferos y plantas entre otros, pero existe un vacío en lo que respecta a la diversidad fúngica. Esto puede deberse a la limitada cultura micofaga en el país. En otros países como España, Finlandia, Noruega y Suecia, existen sitios online que albergan descripciones, imágenes, mapas de distribución y listados con especies en peligro de extinción, satisfaciendo las necesidades tanto de la comunidad científica como también de aquellos que simplemente tienen interés por la flora fúngica. Dada la inmensa variedad de ambientes y escenarios que reúne la Argentina, es necesaria una base de datos online que reúna información de los hongos nativos, es por esto que en 2011 se comenzó el proyecto hongosdeargentina.com.ar con el objetivo de reunir imágenes de hongos encontradas en la Argentina, descripciones relevantes de las especies, comentarios de especialistas, región de distribución geográfica, importancia culinaria y tóxica, entre o...
The aim of this work was to evaluate the potential of grape stalks, an agroindustrial waste, for ... more The aim of this work was to evaluate the potential of grape stalks, an agroindustrial waste, for growth and lignocellulolytic enzyme production via solid-state fermentation, using the following three white rot fungi: Trametes trogii, Stereum hirsutum and Coriolus antarcticus. The decolorization of several dyes by the above mentioned cultures was also investigated. Similar values of dry weight loss of the substrate were measured after 60 days (33-43 %). C. antarcticus produced the highest laccase and Mn-peroxidase activities (33.0 and 1.6 U/g dry solid). The maximum endoglucanase production was measured in S. hirsutum cultures (10.4 U/g), while the endoxylanase peak corresponded to T. trogii (14.6 U/g). The C. antarcticus/grape stalk system seems potentially competitive in bioremediation of textile processing effluents, attaining percentages of decolorization of 93, 86, 82, 82, 77, and 58% for indigo carmine, malachite green, azure B, remazol brilliant blue R, crystal violet and xyli...
International Biodeterioration & Biodegradation, 2011
Trametes trogii BAFC 463 culture fluids (containing 110 U ml À1 laccase; 0.94 U ml À1 manganese p... more Trametes trogii BAFC 463 culture fluids (containing 110 U ml À1 laccase; 0.94 U ml À1 manganese peroxidase), as well as its purified laccase were capable of decolorizing azoic, indigoid, triphenylmethane, anthraquinonic and heterocyclic dyes, in the absence of redox mediators. Six dyes: RBBR, Indigo Carmine, Xylidine, Malachite Green, Gentian Violet and Bromophenol Blue were almost completely degraded (more than 85% decolorization after 1 d) by either laccase or T. trogii itself in culture, proving the role of the enzyme in dye decolorization. The purified laccase also decolorized 65% of Fast Blue RR and 30% of Azure B and Methylene Blue after 24 h. The use of redox mediators significantly increased the decolorization rates (90% decolorization of Azure B after 1 h). 1-hydroxybenzotriazole resulted the best redox mediator, but the natural mediator p-hydroxybenzoic acid also demonstrated its efficiency for dye decolorization. Due to their ability to decolorize recalcitrant dyes without addition of redox mediators, high laccase activities, high thermostability and efficient decolorization at 70 C and pH 7.0, even in the presence of high concentrations of heavy metals (100 mM Cu þ2 , Pb þ2 or Cd þ2 ) or in a synthetic dyebath, T. trogii culture fluids could be effectively used to decolorize synthetic dyes from effluents.
International Biodeterioration & Biodegradation, 2012
Culture filtrates from the white rot fungus Trametes trogii were used for the degradation of two ... more Culture filtrates from the white rot fungus Trametes trogii were used for the degradation of two azo dyes: Xylidine and Methyl Orange. These culture filtrates showed a great potential for decolorizing even high concentrations of both azo dyes, without the addition of redox mediators, possibly due to the synergistic action of their high contents of laccase (104 U ml À1 ), accompanied by Mn-peroxidase (0.54 U ml À1 ), cellobiose dehydrogenase (0.45 U ml À1 ) and glioxal oxidase (0.38 U ml À1 ) activities. 75% of Methyl Orange (980 ppm) and 96% of Xylidine (480 ppm) were degraded after 24 h, degradation occurred after 6 days with a 94% of Xylidine removed (1440 ppm). Biodegradation products from Xylidine were determined by capillary electrophoresis followed by MALDI-TOF mass spectra. Neither aromatic amines nor colored quinones were detected, major metabolites being naphtalene sulphonate and xylene.
La mejor forma de entender lo que tenemos y aprovecharlo, es conociendo lo que nos rodea, estudia... more La mejor forma de entender lo que tenemos y aprovecharlo, es conociendo lo que nos rodea, estudiarlo y aplicarlo. Bajo estas premisas, los hongos son un eslabón muy importante de la biodiversidad, que en este número nos impactan no sólo con sus formas y colores sino también desde el punto de vista de su aplicación en procesos productivos. El estudio de enzimas que producen los hongos para poder llevar adelante procesos productivos que se vinculan directamente con el desarrollo sustentable aprovechando los recursos biológicos de forma inteligente, nos muestra cómo es posible ir más allá del monte aprovechando una de sus infinitas herramientas escondida en la biodiversidad. El camino a seguir para lograr un aprovechamiento de los recursos existentes en nuestros bosques, con el agregado de valor de nuestros investigadores está marcado. Los aportes de la Dra. Laura Villalba y del Lic. Emanuel Grassi sobre sus experiencias en el CIAR y en el desarrollo de biotecnologías en base a estos o...
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