IOP Conference Series: Materials Science and Engineering, 2021
We report the potential antibiofilm properties of a chemically modified biopolymer on the formati... more We report the potential antibiofilm properties of a chemically modified biopolymer on the formation of biofilms by Sphingomonas paucimobilis MG6 an opportunist pathogen isolated from distributed drinking water systems. The biopolymer obtained from the producer bacteria K. terrigena was quarternized and examined for safety using RAW 264.7 cell lines. The modified biopolymer (N-methyl biopolymer: NMB) at 1mg/ml inactivated AHL homologs of S. paucimobilis at ambient temperature. GC-MS and C. violaceum biosensor assays were used to evaluate loss of AHL molecules. On NMB coated coupons loss of biofilm formation under static conditions, was visualized by a rapid calcofluor assays and scanning electron micrographs (SEM). Applicability was further investigated in a flow through apparatus afforded with different plumbing materials, coated with NMB. Leaching of NMB was not observed and a complete absence of biofilms was noted on coated materials, especially in PVC. SEM and viable counts corro...
This study reports the applicability of a capacitance-based technique for evaluating the biofilm ... more This study reports the applicability of a capacitance-based technique for evaluating the biofilm progression of Sphingomonas sp. One hundred and forty isolates of Sphingomonas were screened from public drinking water sites, and one potential strain with biofilm-forming ability was used for the study. The biofilm production by this strain was established in microtiter plates and aluminum coupons. The standard biofilm-forming strain Sphingomonas terrae MTCC 7766 was used for comparison. Changes in biofilm were analyzed by energy-dispersive X-ray spectroscopy (EDX) and scanning electron microscope (SEM). Capacitance values were measured at 1, 100 and 200 kHz frequency; however, 1 kHz was selected since resulted in reproducible values, which could be correlated to biofilm age measured as dry weight over a time of 96 h (4 days) depicting the biofilm growth/progression over time. The EDX, SEM and capacitance values obtained in parallel indicated the related physiological profile usually d...
IOP Conference Series: Materials Science and Engineering, 2021
We report the potential antibiofilm properties of a chemically modified biopolymer on the formati... more We report the potential antibiofilm properties of a chemically modified biopolymer on the formation of biofilms by Sphingomonas paucimobilis MG6 an opportunist pathogen isolated from distributed drinking water systems. The biopolymer obtained from the producer bacteria K. terrigena was quarternized and examined for safety using RAW 264.7 cell lines. The modified biopolymer (N-methyl biopolymer: NMB) at 1mg/ml inactivated AHL homologs of S. paucimobilis at ambient temperature. GC-MS and C. violaceum biosensor assays were used to evaluate loss of AHL molecules. On NMB coated coupons loss of biofilm formation under static conditions, was visualized by a rapid calcofluor assays and scanning electron micrographs (SEM). Applicability was further investigated in a flow through apparatus afforded with different plumbing materials, coated with NMB. Leaching of NMB was not observed and a complete absence of biofilms was noted on coated materials, especially in PVC. SEM and viable counts corro...
This study reports the applicability of a capacitance-based technique for evaluating the biofilm ... more This study reports the applicability of a capacitance-based technique for evaluating the biofilm progression of Sphingomonas sp. One hundred and forty isolates of Sphingomonas were screened from public drinking water sites, and one potential strain with biofilm-forming ability was used for the study. The biofilm production by this strain was established in microtiter plates and aluminum coupons. The standard biofilm-forming strain Sphingomonas terrae MTCC 7766 was used for comparison. Changes in biofilm were analyzed by energy-dispersive X-ray spectroscopy (EDX) and scanning electron microscope (SEM). Capacitance values were measured at 1, 100 and 200 kHz frequency; however, 1 kHz was selected since resulted in reproducible values, which could be correlated to biofilm age measured as dry weight over a time of 96 h (4 days) depicting the biofilm growth/progression over time. The EDX, SEM and capacitance values obtained in parallel indicated the related physiological profile usually d...
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Papers by parul gulati