Sanjai Saxena

Thapar University, Patiala, BIOTECHNOLOGY, Faculty Member

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Phone: +91 9888219815
Address: Professor,
Department of Biotechnology,
Thapar University, Thapar University Campus
Bhadson Road, Patiala 147004 INDIA

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Anna M . Czarnecka

Kumar Saurav

University of Haifa

M. Shakeel Khan

University of Karachi

Neelam Jain

Amity University Rajasthan

Hanaa Ahmed

Animal Health Research Institute

Prof. Tofazzal Islam

Bangabandhu Sheikh Mujibur Rahman Agricultural University

Professor Dr. Loutfy H . Madkour

Al-Baha University, Kingdom of Saudi Arabia

Alaa Al-Charrakh

University of Babylon

El hassan Mayad

Ibn Zohr University, Agadir, Morocco

Nicolas Biais

Brooklyn College of CUNY

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Papers by Sanjai Saxena

Microbial Clot Busters: An Overview of Source, Production, Properties and Fibrinolytic Activity

Heart-related diseases such as high blood pressure, myocardial infarction and ischaemic heart dis... more Heart-related diseases such as high blood pressure, myocardial infarction and ischaemic heart diseases are primarily caused by accumulation of fibrin in the walls of the blood vessels resulting in the formation of a haemostatic plug or a clot. In such conditions, thrombolytic therapy is considered as the gold standard to obtain recanalisation of blood flow in partially occluded veins and arteries. Thrombolytic drugs like streptokinase, alteplase, reteplase and tenecteplase are intravenously administered to recanalise the occluded arteries and veins. Streptokinase is the oldest and most commonly used fibrinolytic agent worldwide. The isolation of streptokinase from β-haemolytic streptococci of Lancefield groups A, C and G is considered as a landmark discovery in thrombolytic therapy which has paved the way for finding novel thrombolytic molecules from microbial resources. Over the years, many novel thrombolytic agents have been discovered from a variety of microbial entities like bac...

Muscodor camphora, a new endophytic species from Cinnamomum camphora

Mycosphere, 2017

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Potential fibrinolytic activity of an endophytic Lasiodiplodia pseudotheobromae species

3 Biotech, 2016

Cost-effective, fibrinolytic agents possessing least or no antigenic properties are much in deman... more Cost-effective, fibrinolytic agents possessing least or no antigenic properties are much in demand for their prospective use as clot busters in thrombolytic therapy. The present communication explores the potential of 22 endophytic Botryosphaeria species isolated from stem of Aegle marmelos for their fibrinolytic potential. Only nine fungal isolates exhibited proteolytic activity out of which only four possessed plasminogen-independent fibrinolytic activity. The endophytic fungal isolate #1088 AMSTITYEL showed maximum in vitro proteolytic and fibrinolytic activity with a halo formation of 153.86 and 113.04 mm 2 , respectively. The partially purified protein exhibited a fibrinolytic activity equivalent to 6.51 U/ml of plasmin. SDS-PAGE of the dialysed fraction of #1088 AMSTITYEL resolved into six bands ranging from 26 to 80 kDa. Fibrin zymogram exhibited that a single band of molecular size *80 KDa possessing the fibrinolytic activity. Furthermore, the bioactive isolate #1088 AMSTITYEL was identified as Lasiodiplodia pseudotheobromae using classical and molecular taxonomic tools.

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Potential pancreatic lipase inhibitory activity of an endophyticPenicilliumspecies

Journal of Enzyme Inhibition and Medicinal Chemistry, 2014

Detailed phytochemical investigation from the root bark of Morus alba resulted in the isolation o... more Detailed phytochemical investigation from the root bark of Morus alba resulted in the isolation of eleven new compounds, including seven 2-arylbenzofuran derivatives (morusalfurans A-G), three flavonoids (morusalnols A-C), and one geranylated stilbene (morusibene A), as well as 22 known compounds. The structures of the identified compounds were elucidated based on a comprehensive analysis of spectroscopic data and Mosher's method. Compounds 2, 3, 6-8, 11, 23, 24, and 29 showed potent inhibition of PL in comparison with the positive control treatment (orlistat, IC 50 = 0.012 lM), with IC 50 values ranging from 0.09 to 0.92 lM.

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Preliminary evaluation of fungal metabolites as natural herbicides for the management of Lantana camara

Indian Phytopathology, 2000

Secondary metabolites of Fusarium oxysporum, Alternaria alternata, Sclerotium rolfsii [Corticium ... more

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Erratum to: Potential fibrinolytic activity of an endophytic Lasiodiplodia pseudotheobromae species

3 Biotech, 2016

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Anti-Staphylococcal potential of Callistemon rigidus

The last decade witnessed the emergence of Staphylococcus aureus- a versatile human pathogen, as ... more The last decade witnessed the emergence of Staphylococcus aureus- a versatile human pathogen, as a deadly superbug. The enormous genetic plasticity of the organism assists it to endlessly evolve resistance mechanisms against existing anti-infectives thus necessitating the need to control the spread of resistant staphylococcal isolates in hospitals and health care settings. This in turn demands the incessant exploration of newer biological matrices in search of diverse chemical entities with novel drug targets. Since time immemorial higher plants continue to be the best source of newer compounds with high therapeutic potential. Lead fractions from same or different plants can be developed into effective antibacterial polyherbal formulations. A lead fraction from methanolic extract of leaves of Callistemon rigidus exhibited a dose dependent antistaphylococcal activity during in vitro agar well assay against a panel of twenty seven clinical multidrug resistant S. aureus isolates. Furth...

Assessment of Phytotoxic Spent Broth and Spores of Alternaria Alternata (LC# 508) As a Formulation with Enhanced Mycoherbicidal Activity to Control Lantana …

Journal of Plant Protection Research, Mar 1, 2009

Abstract: Enhanced social concern towards the ill effects of synthetic agrochemicals, their resid... more

Mycoherbicidal potential of Alternaria alternata ITCC 4896 for the control of Parthenium hysterophorus

Mycoherbicides are special biotechnology products which contain fungi or fungal metabolites as no... more Mycoherbicides are special biotechnology products which contain fungi or fungal metabolites as nonchemical alternatives thereby reducing the input of harmful chemicals to control noxious weeds. The present communication emphasizes on the potential of an indigenous isolate of Alternaria alternata ITCC 4896 as a mycoherbicide for the global weed – Parthenium hysterophorus. Of the various spore concentrations tested by in vitro Detached Leaf Bioassay, 1x106 spores/ml was the most effective inducing 89.2% leaf area damage on the 7th day and was further tested by Whole Plant Bioassay. Both in vitro Detached Leaf Bioassay and Whole Plant Bioassay exhibited a similar trend in disease development showing 50% damage at 96 hours post treatment. However, 100% mortality was observed in the Whole Plant Bioassay on the 7th day. This is the very first report on the bioweedicidal potential of A. alternata ITCC 4896 (LC#508) for use as a mycoherbicide for P. hysterophorus.

In vitro Anti-staphylococcal Potential of Endophytic Fungi from Aegle marmelos Vinit Meshram, Sanjai Saxena* and Neha Kapoor

Journal of pure and applied microbiology

ABSTRACT Staphylococcus aureus is the third most dreaded human pathogen which is getting refracto... more ABSTRACT Staphylococcus aureus is the third most dreaded human pathogen which is getting refractory to current armamentarium of antimicrobial drugs. Endophytic fungi have been recognized as a fountainhead of novel bioactives for human therapeutic intervention and drug development. In the present investigation we report isolation and screening of endophytic fungi isolated from Aegle marmelos inhabiting conserved forest area in Western Ghats, India. A total of 25 endophytic fungi representing 10 genera were isolated by adopting a standardized protocol. Fusarium species exhibited maximum colonization (28%), followed by Aureobasidium (12%) and Lasiodiplodia species (8%). In vitro screening of culture broth of these fungi using a standardized anti-microbial assay exhibited activity only in one isolate #1005AMLBRT. Subsequently ethyl acetate extracts of the culture broths revealed that 15 isolates did not possess any anti- staphylococcal potential. However the best anti-staphylococcal potential was found in ethyl acetate extract of #1005AMLBRT. #1005AMLBRT was found to be a new species of Alternaria named as Alternaria marmelos based on phylogenetic and morphological studies. Further studies on Alternaria marmelos are warranted for production, isolation and characterization of the anti-staphylococcal principle

Xanthine oxidase inhibitors from an endophytic fungus Lasiodiplodia pseudotheobromae

Bioorganic Chemistry

Two new compounds, lasdiplactone (1) and lasdiploic acid (2) and one known compound 3 were isolat... more Two new compounds, lasdiplactone (1) and lasdiploic acid (2) and one known compound 3 were isolated from the chloroform extract of cell free filtrate of the endophytic fungus Lasiosdiplodia pseudotheobromae. The structures of new compounds were determined by interplay of spectral techniques (IR, mass, H NMR, C NMR, DEPT, and 2D NMR). The absolute configuration at C-4 position of 1 was established as S using a process similar to modified Mosher&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s method. The absolute configuration of 2 was established by comparing its ECD spectrum with the calculated ECD spectra of all possible isomers. In the in vitro XO inhibition assay, the highest inhibition was exhibited by 3 with an IC of 0.38 ± 0.13 μg/ml, followed by 2 with an IC of 0.41 ± 0.1 μg/ml and the least in 1. The oxidized form of 1 also showed high XO inhibition with IC of 0.35 ± 0.13 μg/ml.

Endophytic Fungi: A Source of Potential Antifungal Compounds

Journal of fungi (Basel, Switzerland), Jan 25, 2018

The emerging and reemerging forms of fungal infections encountered in the course of allogeneic bo... more The emerging and reemerging forms of fungal infections encountered in the course of allogeneic bone marrow transplantations, cancer therapy, and organ transplants have necessitated the discovery of antifungal compounds with enhanced efficacy and better compatibility. A very limited number of antifungal compounds are in practice against the various forms of topical and systemic fungal infections. The trends of new antifungals being introduced into the market have remained insignificant while resistance towards the introduced drug has apparently increased, specifically in patients undergoing long-term treatment. Considering the immense potential of natural microbial products for the isolation and screening of novel antibiotics for different pharmaceutical applications as an alternative source has remained largely unexplored. Endophytes are one such microbial community that resides inside all plants without showing any symptoms with the promise of producing diverse bioactive molecules ...

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Process for producing herbicides from a fungus alternaria alteranata f.sp. lantanae

Abstract not available for EP1553834.

Glycrrhiza glabra: Medicine over the millennium

Natural Product Radiance, Mar 24, 2005

A Process for Producing Herbicides from a Fungus Alternaria Alteranata F.SP. Lantanae

Abstract not available for EP1553834.

Microbial Biotransformation of Cannabinoids

... He has always been an inspiration to me for his hard work and efforts to excel. I am thankful... more

Fungal biotransformation of cannabinoids: potential for new effective drugs

Current opinion in drug discovery & development, 2009

Phytocannabinoids from the plant Cannabis sativa induce a variety of physiological and pharmacolo... more Phytocannabinoids from the plant Cannabis sativa induce a variety of physiological and pharmacological responses in living systems, including anti-inflammatory, antinociceptive, anti-ulcer and antitumor activities. The discovery of the cannabinoid receptors CB1 and CB2 led to the development of agonists and antagonists of these receptors for the treatment of a variety of diseases. Nabilone, a synthetic derivative of Delta9-tetrahydrocannabinol (Delta9-THC), which is the main natural psychotropic constituent of C sativa, was approved by the US FDA for the treatment of nausea and as an anti-emetic for patients undergoing chemotherapy. Delta9-THC and related cannabinoids are involved in a variety of signal transduction pathways; thus, reducing or removing the psychotropic effects of these compounds would enhance their therapeutic spectra. Compound synthesis and qualitative SAR studies are time-consuming activities; however, microbes are effectively the most inventive synthetic chemists...

Microbes in Production of Fine Chemicals (Antibiotics, Drugs, Vitamins, and Amino Acids)

Applied Microbiology, 2015

Potential Xanthine Oxidase Inhibitory Activity of Endophytic Lasiodiplodia pseudotheobromae

Applied Biochemistry and Biotechnology, 2014

Xanthine oxidase is considered as a potential target for treatment of hyperuricemia. Hyperuricemi... more Xanthine oxidase is considered as a potential target for treatment of hyperuricemia. Hyperuricemia is predisposing factor for gout, chronic heart failure, atherosclerosis, tissue injury, and ischemia. To date, only two inhibitors of xanthine oxidase viz. allopurinol and febuxostat have been clinically approved for used as drugs. In the process of searching for new xanthine oxidase inhibitors, we screened culture filtrates of 42 endophytic fungi using in vitro qualitative and quantitative XO inhibitory assays. The qualitative assay exhibited potential XO inhibition by culture filtrates of four isolates viz. #1048 AMSTITYEL, #2CCSTITD, #6AMLWLS, and #96 CMSTITNEY. The XO inhibitory activity was present only in the chloroform extract of the culture filtrates. Chloroform extract of culture filtrate #1048 AMSTITYEL exhibited the highest inhibition of XO with an IC50 value of 0.61 μg ml(-1) which was better than allopurinol exhibiting an IC50 of 0.937 μg ml(-1) while febuxostat exhibited a much lower IC50 of 0.076 μg ml(-1). Further, molecular phylogenetic tools and morphological studies were used to identify #1048 AMSTITYEL as Lasiodiplodia pseudotheobromae. This is the first report of an endophytic Lasiodiplodia pseudotheobromae from Aegle marmelos exhibiting potential XO Inhibitory activity.

In vitro Anti-staphylococcal Potential of Endophytic Fungi from Aegle marmelos

Journal of Pure and Applied Microbiology

Staphylococcus aureus is the third most dreaded human pathogen which is getting refractory to cur... more Staphylococcus aureus is the third most dreaded human pathogen which is getting refractory to current armamentarium of antimicrobial drugs. Endophytic fungi have been recognized as a fountainhead of novel bioactives for human therapeutic intervention and drug development. In the present investigation we report isolation and screening of endophytic fungi isolated from Aegle marmelos inhabiting conserved forest area in Western Ghats, India. A total of 25 endophytic fungi representing 10 genera were isolated by adopting a standardized protocol. Fusarium species exhibited maximum colonization (28%), followed by Aureobasidium (12%) and Lasiodiplodia species (8%). In vitro screening of culture broth of these fungi using a standardized anti-microbial assay exhibited activity only in one isolate #1005AMLBRT. Subsequently ethyl acetate extracts of the culture broths revealed that 15 isolates did not possess any anti- staphylococcal potential. However the best anti-staphylococcal potential wa...

Microbial Clot Busters: An Overview of Source, Production, Properties and Fibrinolytic Activity