PIYUSH JOSHI
Supervisors: Dr. Laxi Kant, Dr. G.Selvakumar, and Dr. G.K. Joshi
Address: Senior Scientific Officer,
Uttarakhand State Council for Science and Technology (UCOST),
VIGYAN DHAM, Jhjara, Premnagar
Dehradun - 248007, Uttarakhand, INDIA
Address: Senior Scientific Officer,
Uttarakhand State Council for Science and Technology (UCOST),
VIGYAN DHAM, Jhjara, Premnagar
Dehradun - 248007, Uttarakhand, INDIA
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Papers by PIYUSH JOSHI
unavoidable waste generated as a resource and achieve higher levels of recycling and safe disposal. Post harvest
losses contribute to a substantial proportion of the loss that the agricultural biomass undergoes
in India. It is therefore important to make an assessment of this loss and assign a certain cost to it. In this
study, we have carried out an assessment of the residues that are generated in the field or on the farm at
the time of harvest (for example wheat and rice straw), wastes generated as a result of post-harvest losses.
In addition, the by-products from the processing of agricultural produce (for example sugarcane bagasse
produced during the production of sugar from sugar cane, or cereal husks produced during milling) are
also considered. Finally, certain aspects of the environmental impact and sustainability of the utilization
of agricultural residues and by-products are addressed.
Pantoea dispersa strain 1A is a Gram-negative rod-shaped, yellow-pigmented bacterium isolated on nutrient agar plates incubated at 4°C. The identity of the bacterium was confirmed by sequencing of the 16 S rRNA gene. It was capable of growing at temperatures ranging from 4 to 42°C, but maximum growth was observed at 30°C. It is endowed with multiple plant growth promotion attributes such as phosphate solubilization, IAA production, siderophore production and HCN production, which are expressed differentially at sub-optimal temperatures (15 and 4°C). It was able to solubilize phosphate (17.6 μg of P2O5 ml−1 day−1), and produce IAA (3.7 μg ml−1 day−1), at 15°C. Qualitative detection of siderophore production and HCN were also observed at 15°C. At 4°C it was found to express all the plant growth promotion attributes. This bacterial isolate was able to positively influence and promote the growth and nutrient uptake parameters of wheat (cv. VL.802) under glasshouse conditions. Hence in the context, of cold wheat-growing environments, it is proposed that Pantoea dispersa 1A (MTCC 8706), could be deployed as an inoculant to attain the desired results of bacterization.
Keywords Cold tolerance - Nutrient uptake - Pantoea dispersa - Plant growth promotion
Phosphate solubilization and growth promotion by Pseudomonas fragi CS11RH1 (MTCC 8984), a psychrotolerant bacterium isolated from a high altitude garlic rhizosphere from the Indian Himalayas, are reported here. The identity of the isolate was arrived on the basis of its biochemical features and sequencing of the 16S rRNA gene. The isolate grew and solubilized phosphate at temperatures ranging from 4 to 30°C. Besides solubilizing P it produced indole acetic acid (IAA) and hydrogen cyanide (HCN). Seed bacterization with the isolate significantly increased the percent germination, rate of germination, plant biomass and nutrient uptake of wheat seedlings. While Pseudomonas fragi is normally associated with the spoilage of dairy products stored at cold temperatures, this is an early report on the plant growth promoting ability of the bacterium.
Key words psychrotolerant - Pseudomonas fragi - phosphate solubilization - plant growth promotion
unavoidable waste generated as a resource and achieve higher levels of recycling and safe disposal. Post harvest
losses contribute to a substantial proportion of the loss that the agricultural biomass undergoes
in India. It is therefore important to make an assessment of this loss and assign a certain cost to it. In this
study, we have carried out an assessment of the residues that are generated in the field or on the farm at
the time of harvest (for example wheat and rice straw), wastes generated as a result of post-harvest losses.
In addition, the by-products from the processing of agricultural produce (for example sugarcane bagasse
produced during the production of sugar from sugar cane, or cereal husks produced during milling) are
also considered. Finally, certain aspects of the environmental impact and sustainability of the utilization
of agricultural residues and by-products are addressed.
Pantoea dispersa strain 1A is a Gram-negative rod-shaped, yellow-pigmented bacterium isolated on nutrient agar plates incubated at 4°C. The identity of the bacterium was confirmed by sequencing of the 16 S rRNA gene. It was capable of growing at temperatures ranging from 4 to 42°C, but maximum growth was observed at 30°C. It is endowed with multiple plant growth promotion attributes such as phosphate solubilization, IAA production, siderophore production and HCN production, which are expressed differentially at sub-optimal temperatures (15 and 4°C). It was able to solubilize phosphate (17.6 μg of P2O5 ml−1 day−1), and produce IAA (3.7 μg ml−1 day−1), at 15°C. Qualitative detection of siderophore production and HCN were also observed at 15°C. At 4°C it was found to express all the plant growth promotion attributes. This bacterial isolate was able to positively influence and promote the growth and nutrient uptake parameters of wheat (cv. VL.802) under glasshouse conditions. Hence in the context, of cold wheat-growing environments, it is proposed that Pantoea dispersa 1A (MTCC 8706), could be deployed as an inoculant to attain the desired results of bacterization.
Keywords Cold tolerance - Nutrient uptake - Pantoea dispersa - Plant growth promotion
Phosphate solubilization and growth promotion by Pseudomonas fragi CS11RH1 (MTCC 8984), a psychrotolerant bacterium isolated from a high altitude garlic rhizosphere from the Indian Himalayas, are reported here. The identity of the isolate was arrived on the basis of its biochemical features and sequencing of the 16S rRNA gene. The isolate grew and solubilized phosphate at temperatures ranging from 4 to 30°C. Besides solubilizing P it produced indole acetic acid (IAA) and hydrogen cyanide (HCN). Seed bacterization with the isolate significantly increased the percent germination, rate of germination, plant biomass and nutrient uptake of wheat seedlings. While Pseudomonas fragi is normally associated with the spoilage of dairy products stored at cold temperatures, this is an early report on the plant growth promoting ability of the bacterium.
Key words psychrotolerant - Pseudomonas fragi - phosphate solubilization - plant growth promotion