Papers by Srinivasan M Rajaram
The Pharma Innovation Journal, 2021
Andrographis paniculata (Burm.f.) Nees. is a medicinal herb widely used in various traditional me... more Andrographis paniculata (Burm.f.) Nees. is a medicinal herb widely used in various traditional medicines for the treatment of various ailments particularly related to viral fever, respiratory tract infections, and other pharmacological properties. Andrographolide is a diterpene present as a major active ingredient responsible for various pharmacological activities. This study was conducted to assess the mutagenicity and clastogenicity potential of the andrographolide by Ames mutagenicity and in vitro chromosomal aberration studies, respectively. Materials and Methods: Ames Mutagenicity assay was performed using Salmonella typhimurium strains, TA100, TA1535, TA102 and TA98 and TA1537 and in vitro chromosomal aberration study was performed in CHO-K1 cell line. Both these studies were conducted following the procedure mentioned in the OECD guidelines no. 471 and 473 for Ames and in vitro chromosomal aberration studies, respectively. Results: Andrographolide was found to be non-mutagenic in Ames mutagenicity assay and nonclastogenic in the in vitro chromosomal aberration study in the CHO-K1 cell line.
The colossal rise in antimicrobial resistance has led to treatment failures and so mastitis has b... more The colossal rise in antimicrobial resistance has led to treatment failures and so mastitis has become cumbersome to treat. The objective of this study was to evaluate the antibacterial effect of non-antibiotic drug, atorvastatin in combination with antimicrobial, ampicillin against two commonly isolated bacterial species Staphylococcus spp and E. coli from bovine mastitis. Milk samples were collected from mastitis cows, visiting Veterinary Clinical Complex. Bacterial isolation was performed using Eosin Methylene Blue (EMB) agar and Mannitol Salt Agar (MSA), followed by characterization and identification by biochemical tests and gram staining. Genotypic confirmation was done by Polymerase Chain Reaction (PCR) with subsequent screening for resistant genes-mec A, blaTEM. Antibiotic Sensitivity Test (ABST) of the isolates against 12 different antimicrobials, atorvastatin only, and combination of atorvastatin with ampicillin were performed using Kirby-Bauer disc diffusion method. Minim...
The Pharma Innovation Journal, 2021
Andrographis paniculata (Burm.f.) Nees. is a medicinal herb widely used in various traditional me... more Andrographis paniculata (Burm.f.) Nees. is a medicinal herb widely used in various traditional medicines for the treatment of various ailments particularly related to viral fever, respiratory tract infections, and other pharmacological properties. Andrographolide is a diterpene present as a major active ingredient responsible for various pharmacological activities. This study was conducted to assess the mutagenicity and clastogenicity potential of the andrographolide by Ames mutagenicity and in vitro chromosomal aberration studies, respectively. Materials and Methods: Ames Mutagenicity assay was performed using Salmonella typhimurium strains, TA100, TA1535, TA102 and TA98 and TA1537 and in vitro chromosomal aberration study was performed in CHO-K1 cell line. Both these studies were conducted following the procedure mentioned in the OECD guidelines no. 471 and 473 for Ames and in vitro chromosomal aberration studies, respectively. Results: Andrographolide was found to be non-mutagenic in Ames mutagenicity assay and nonclastogenic in the in vitro chromosomal aberration study in the CHO-K1 cell line.
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Papers by Srinivasan M Rajaram