The use of 5-fluorouracil (5-FU) is associated with multifaceted challenges and poor pharmacokine... more The use of 5-fluorouracil (5-FU) is associated with multifaceted challenges and poor pharmacokinetics. Accordingly, our study was designed to prepare 5-FU nanogel as a new form of the colon cancer chemotherapeutic drug 5-FU using polyacrylic acid and gelatin hybrid nanogel as efficient drug carriers. Alongside the in vivo chemotherapeutic evaluation, the anti-proliferative and anti-apoptotic efficacy were carried out for 5-FU nanogel against 1,2-dimethylhydrazine (DMH, 20 mg/kg) and γ-radiation (4 Gy)-prompted colon dysplasia in rats compared to 5-FU. The morphology and size of 5-FU nanogel were characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS) in addition to cytotoxicity assay. The expression of phosphoinositide-3-kinase (PI3K)/Akt, mammalian target of rapamycin (mTOR); Toll-like receptor2 (TLR2)/nuclear factor kappa B), adenosine monophosphate (AMP)-activated protein kinase (AMPK) and its downstream autophagy-related genes in addition to ap...
www.alexjvs.com Retrospective study of teat surgical affections in dairy farms of armed forces an... more www.alexjvs.com Retrospective study of teat surgical affections in dairy farms of armed forces and their treatment
Objective: In the present study, the effect of selenium nanoparticles (SeNPs) and ferulic acid (F... more Objective: In the present study, the effect of selenium nanoparticles (SeNPs) and ferulic acid (FA) on acrylamide (AC) induced neurotoxicity in rats was investigated. Methods: Sixty adult male rats were divided equally into six groups and orally treated as follows: Group I (control): 1ml per day physiological saline; group II (SeNPs): 0.5 mg/kg body weight per day SeNPs for 21 days; group III (FA): 100 mg/kg body weight per day FA for 21 days; group IV (AC): 50 mg/kg body weight per day acrylamide for 21 days; group V (AC+SeNPs): AC 50 mg/kg body weight for 21 days then SeNPs as group II. Group VI (AC+ FA): AC 50 mg/kg body weight per day then FA as group III. Results: AC significantly (P<0.05) increased the level of β-amyloid (Aβ) in brain tissue and interleukin 6 (IL-6) in serum and significantly (P<0.05) decreased the level of Brain-Derived Neurotrophic factor (BDNF) in the brain tissue compared to control group. Treatment with FA or SeNPs significantly (P<0.05) decreased the level of Aβ in brain tissue and IL-6 in serum and significantly (P<0.05) increased the level of BNDF in brain tissue compared to acrylamide group. AC induced histopathological changes in brain hippocampus and striatum which were improved with SeNPs and FA treatment. Conclusion: It is concluded from the present study that ferulic acid and SeNPs had a therapeutic effect against neurotoxicity induced by AC in experimental rats.
The use of 5-fluorouracil (5-FU) is associated with multifaceted challenges and poor pharmacokine... more The use of 5-fluorouracil (5-FU) is associated with multifaceted challenges and poor pharmacokinetics. Accordingly, our study was designed to prepare 5-FU nanogel as a new form of the colon cancer chemotherapeutic drug 5-FU using polyacrylic acid and gelatin hybrid nanogel as efficient drug carriers. Alongside the in vivo chemotherapeutic evaluation, the anti-proliferative and anti-apoptotic efficacy were carried out for 5-FU nanogel against 1,2-dimethylhydrazine (DMH, 20 mg/kg) and γ-radiation (4 Gy)-prompted colon dysplasia in rats compared to 5-FU. The morphology and size of 5-FU nanogel were characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS) in addition to cytotoxicity assay. The expression of phosphoinositide-3-kinase (PI3K)/Akt, mammalian target of rapamycin (mTOR); Toll-like receptor2 (TLR2)/nuclear factor kappa B), adenosine monophosphate (AMP)-activated protein kinase (AMPK) and its downstream autophagy-related genes in addition to ap...
www.alexjvs.com Retrospective study of teat surgical affections in dairy farms of armed forces an... more www.alexjvs.com Retrospective study of teat surgical affections in dairy farms of armed forces and their treatment
Objective: In the present study, the effect of selenium nanoparticles (SeNPs) and ferulic acid (F... more Objective: In the present study, the effect of selenium nanoparticles (SeNPs) and ferulic acid (FA) on acrylamide (AC) induced neurotoxicity in rats was investigated. Methods: Sixty adult male rats were divided equally into six groups and orally treated as follows: Group I (control): 1ml per day physiological saline; group II (SeNPs): 0.5 mg/kg body weight per day SeNPs for 21 days; group III (FA): 100 mg/kg body weight per day FA for 21 days; group IV (AC): 50 mg/kg body weight per day acrylamide for 21 days; group V (AC+SeNPs): AC 50 mg/kg body weight for 21 days then SeNPs as group II. Group VI (AC+ FA): AC 50 mg/kg body weight per day then FA as group III. Results: AC significantly (P<0.05) increased the level of β-amyloid (Aβ) in brain tissue and interleukin 6 (IL-6) in serum and significantly (P<0.05) decreased the level of Brain-Derived Neurotrophic factor (BDNF) in the brain tissue compared to control group. Treatment with FA or SeNPs significantly (P<0.05) decreased the level of Aβ in brain tissue and IL-6 in serum and significantly (P<0.05) increased the level of BNDF in brain tissue compared to acrylamide group. AC induced histopathological changes in brain hippocampus and striatum which were improved with SeNPs and FA treatment. Conclusion: It is concluded from the present study that ferulic acid and SeNPs had a therapeutic effect against neurotoxicity induced by AC in experimental rats.
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Papers by Wael Barakat