India is well known for its rich biological diversity. It is endowed with the rich wealth of flor... more India is well known for its rich biological diversity. It is endowed with the rich wealth of flora and fauna. Many plants contain medicinal properties, which helps to cure the diseases which have a severe impact on mankind. Nearly, 3000 medicinal plants were recognized commercially in India. In the present study, the phytochemical properties and the medicinal values of important 2 plants and 3 trees available in India are compared. The significant presence of compounds such as flavonoids, tannins, saponins, alkaloids, glycosides etc present in various sources of aqueous and methanolic extracts like Boerhaavia diffusa, Emblica officinalis, Terminalia chebula, Terminalia bellirica and, Withania sominifera were found by phytochemical screening, which accounts for its various medicinal properties.
— Many multidrug resistant organisms emerged as a notorious problem in contemporary medicine heal... more — Many multidrug resistant organisms emerged as a notorious problem in contemporary medicine healthcare. Most of the Methicillin Resistant Staphylococcus aureus (MRSA) strains are ubiquitous worldwide and are found to be Multi-Drug Resistant (MDR) due to acquisition of mecA gene encoding Penicillin Binding Protein 2A (PBP2A). The inhibitory activity or interaction of flavonoids like Galangin, Catechin, Luteolin, Quercetin and alkaloid Mimosine from Mimosa pudica against PBP2A receptors of MRSA using molecular docking were studied. Physical properties, amino acid (AA) interactions and drug likeliness of plant molecules were also analyzed. Docking results of all the selected phytoligands were compared with standard antibiotic Linezolid to identify the best leads inhibiting PBP2A of MRSA. Among those selected ligands Mimosine exhibit maximum Docking Score of 115.26 followed by Quercetin (62.95), Luteolin (58.54), Catechin (57.6) and Galangin (51.33) when compared to Linezolid (38.74). Further invitro experimental analysis will be carried out to elucidate the antimicrobial activity of selected lead molecules against MRSA strains.
Liver diseases are the second most predominant diseases next to diabetes. Treatment of most of th... more Liver diseases are the second most predominant diseases next to diabetes. Treatment of most of the liver diseases involves liver transplantation, which is not feasible due to lack of sufficient donors. Most of the drugs prescribed are random and doesn't involve proper action in the patient. Hence targeted delivery of the drug is the most viable and affordable solution for treating liver diseases. Despite many available carriers like viral vectors, metallic vectors etc, liposomes are the most suitable drug carrier molecules and they are more advantageous than other materials. Targeted delivery experiments involve much tedious experimental procedures and complex biological pathway study. Developing and analysing these nano scale compounds are also much difficult and hence for the ease of work, tools of Bioinformatics are used to analyse and interpret the results. Hence in-Silico analysis are commonly used for analysing their pathway before carrying out the in-Vitro and in-Vivo experiments. This review article emphasis on the various procedures and software used in targeted delivery experiments.
The Present Study is to analyse the targeted delivery of Ursodiol, a drug used in the treatment o... more The Present Study is to analyse the targeted delivery of Ursodiol, a drug used in the treatment of Primary Sclerosing Cholangitis (PSC) using Lipid Bilayer Nano-Particles or Solid Lipid Nano Particles(SLNPs) which is taken for insilico binding studies with various 4 different liver receptors. 3 Solid Lipid Nano Particles are chosen and Binding Efficiency of the Drug with Solid Lipid Nano Particles are Studied Using DFT Analysis and FEM Analysis. It was seen that the complete drug binding is seen in Small Unilamellar Vessicle (SUV). Chosen Liver Receptors with Ursodiol of the SUV are checked for binding studies using AutoDock 4. The Study revealed that Liver X Receptor α has a greater efficiency in binding with the Solid Lipid Nano Particles.
The study is to analyse computationally, the Lipid Bilayer Nano-Particles or liposomes mediated, ... more The study is to analyse computationally, the Lipid Bilayer Nano-Particles or liposomes mediated, targeted delivery of Ribavirin, a drug used primarily in the treatment of Cholangiocarcinoma, a Hepatomal disease mainly caused due to infection by liver flukes or by mutation in epithelial cells. For In-Silico binding studies, 5 different liposomes are selected and their properties are studied. Based on the feasibility of the drug-liposome binding that is examined by using MD Simulations, those that meet the criteria for targeted drug delivery are chosen and the binding efficiency with the drug and the lipid nanoparticles are determined. It was seen that the best drug binding affinity is shown for 1, 2 -Distearoyl-sn -glycero-3-phosphocholine (DSPC) liposome. Then Liver Receptors, specific to cholesterol and sterol metabolism are chosen for Binding studies with the above mentioned liposome (capsulated with the drug) docked using LigandFit and screened primarily through dock scores using Ligscore module in Discovery Studio. The study revealed that SREBP-1 Liver Receptor has a greater efficiency in binding with the drugliposome complex and hence can be adjudged to be most suited for targeted drug delivery experiments. W WO OR RL LD D J JO OU UR RN NA AL L O OF F P PH HA AR RM MA AC CY Y A AN ND D P PH HA AR RM MA AC CE EU UT TI IC CA AL L S SC CI IE EN NC CE ES S V Vo ol lu um me e 2 2, , I Is ss su ue e 6 6, , X XX XX X--X XX XX X. . R Re es se ea ar rc ch h A Ar rt ti ic cl le e I
The objective of this project is to apply data analysis techniques to analyze data on periodontal... more The objective of this project is to apply data analysis techniques to analyze data on periodontal disease and discover the factors that were most related to periodontal disease. In This Project a12-month, double-blind, randomized, placebocontrolled clinical study was conducted to determine the efficacy of an active drug on treating periodontal disease.
Derivatives of Indole-type compounds constitute an important class of compounds for new drug deve... more Derivatives of Indole-type compounds constitute an important class of compounds for new drug development in order to discover an effective compound against multi-drug-resistant microbial infections. Experimental studies have shown the antimicrobial analysis of oxindole derivative compounds with clinical bacterial pathogens of humans like Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Proteus mirabilis, Salmonella typhimurium, Salmonella typhi, Salmonella paratyphi 'A', Shigella sonnei and Vibrio cholera. The current study investigates the MICs of six Oxindole derivatives namely 3and 1-N-allyl-3,3-dinaphthyl-oxindole compounds with the eleven bacterial pathogens. The MIC's of six different oxindole compounds are compared with standard antibiotic like Ampicillin dissolved in various concentrations of 100, 150 & 200 µg/ml in Dimethyl Sulfoxide (DMSO). The Oxindole derivative compounds are found to show potent activity against most of the pathogens tested. Most of the derivatives were especially effective against P.aeruginosa, S.typhimurium, E.feacalis and Klebsiella pneumonia. Hence, further research on indole compounds and their derivatives could be studied out for better understanding of their antimicrobial activity using laboratory and computational tools.
Accumulation of excess fat on the body is called as "obesity". A certain amount of body fat is ne... more Accumulation of excess fat on the body is called as "obesity". A certain amount of body fat is necessary for strong energy, heat insulation, shock absorption and other functions. The normal amount of body fat is between 25-30 % in women and 18-23 % in men. Women with over 30% body fat and men with over 25% fat are considered obese. The calculation of body mass index (BMI) has also been used in the definition of obesity. The BMI equals a person's weight in kilograms (Kg) divided by their height in meters (m) squared. Since BMI describes body weight relative to height, it is strongly correlated with total body fat content in adults. Obesity is defined as having a body mass index of greater than 30.Lipoprotein lipase is an enzyme that hydrolyses lipids into two free fatty acids and one mono acyl glycerol molecule. The accumulation of this fat leads to obesity. So lipoprotein lipase plays an important role in obesity. So lipoprotein lipase used as a target protein for obesity. In our project to inhibit lipoprotein lipase activity we used drugs from plant and chemical sources. One drug is derived from drug bank (chemical source) and the other two drugs are derived from pubchem (herbal drugs). We compared and analyzed the activity of these drugs on lipoprotein lipase activity and we found the drug which best inhibits the lipoprotein lipase activity.The procedure includes following steps. They are as follows: Identification of target protein, Prediction of target structure, identification of ligands, docking of target with the ligands,finding the best drug which inhibits the target protein.
The fres h water field crab, Spiralothelphusa hydrodroma is an important human food source in par... more The fres h water field crab, Spiralothelphusa hydrodroma is an important human food source in parts of South In d ia and the crab is cons tantly exposed to pes ticides , which are us ed extens ively to control ag ricu ltu ral pes ts . Evalu atio n o f th e to xic effect o f cy p ermeth rin o n th e exp erimen tal crab fo r th e LC50 v alu e was carried o u t. Effect o f cy p ermeth rin o n th e b io ch emical ch an g es in th e n eu ro s ecreto ry cells s u ch as b rain , thoracic ganglia and eyestalk was observed. Quantitative study of biochemical changes of lactate hydrogenase (LDH), succinate dehydrogenase (SDH), acid phosphatase (ACP) and alkaline phosphatase (ALP) in th e neuros ecretory cells was undertaken.
This paper presents the details of analytical studies carried out by using advanced bioinformatic... more This paper presents the details of analytical studies carried out by using advanced bioinformatics tools on classifying all proteins which are involved in causing dormancy in human & Spermophilus tridecemlineatus a selected animal (Squirrel) and analyzing their structures, keeping their active domains in major focus. The study primarily focused on (i) Identifying genes which are related to dormancy (ii) modeling PDK4 from different organisms (iii) structural analysis to find out the structural similarity and domain details and (iv) PDK4 pathway studies to give a detailed insight into mechanism of action of PDK4. From the studies, it is observed from the studies that squirrel PDK4 shows dormancy as natural adaptation compared to human PDK4. Typical case study of Ramachandran plot of human and squirrel PDK4 has been explained in detail. RJPBCS Volume 4 Issue 1 Page No. 826
The Present Study is to analyse the targeted delivery of Ursodiol, a drug used in the treatment o... more The Present Study is to analyse the targeted delivery of Ursodiol, a drug used in the treatment of Primary Sclerosing Cholangitis (PSC) using Lipid Bilayer Nano-Particles or Solid Lipid Nano Particles(SLNPs) which is taken for insilico binding studies with various 4 different liver receptors. 3 Solid Lipid Nano Particles are chosen and Binding Efficiency of the Drug with Solid Lipid Nano Particles are Studied Using DFT Analysis and FEM Analysis. It was seen that the complete drug binding is seen in Small Unilamellar Vessicle (SUV). Chosen Liver Receptors with Ursodiol of the SUV are checked for binding studies using AutoDock 4. The Study revealed that Liver X Receptor α has a greater efficiency in binding with the Solid Lipid Nano Particles.
The fres h water field crab, Spiralothelphusa hydrodroma is an important human food source in par... more The fres h water field crab, Spiralothelphusa hydrodroma is an important human food source in parts of South India and the crab is cons tantly expos ed to pes ticides , which are us ed extens ively to control ag ricu ltu ral p es ts . Ev alu atio n o f th e to xic effect o f cy p ermeth rin o n th e exp erimen tal crab fo r th e LC50 v alu e was carried out. Effect of cypermethrin on the quantitative study of nutritive value viz. protein, carbohydrate and lipid in ovary, spermatheca, hepatopancreas, muscle, g ills, haemolymph, brain, thoracic ganglia and eyestalk was observed. 50 Ke y wor ds : Protein, carbohydrate, lipid, LC , Cypermethrin, Spiralothelphusa hydrodroma
Boerhavia diffusa is a medicinal plant used in treatment of autoimmune diseases like Rheumatic ar... more Boerhavia diffusa is a medicinal plant used in treatment of autoimmune diseases like Rheumatic arthritis, Alzheimer's disease and Colon cancer. The present study investigates the application of bioinformatics tools showing binding affinity of BoeravinoneE with the human immunomodulatory receptor molecules like GMCSF and TNFα responsible for maturation and activation of dendritic cells. The binding pocket sites, the internal energy, the hydrogen bond interactions and the interacting amino acid residues of the human immunomodulatory protein molecules with BoeravinoneE were analyzed through molecular docking. Among the human immunomodulatory molecules docked, which are responsible for DCs maturation and activation, BoeravinoneE was binding with receptors of GMCSF, and TNFα exhibiting a maximum Dock score of 47.43, 59.31 and internal binding energy of 5.5 J/mol, 0.10 J/mol. BoeravinoneE was docked with binding site aminoacids, LEU246 of GMCSFR and SER74, ASN110 of TNF-αR as predicted from UNIPROT and PDBSum. This present study could also be performed further for checking immunomodulation activity studies in invitro and invivo in mice.
The aim of the paper is to provide a detailed description of the M. tuberculosis proteome by usin... more The aim of the paper is to provide a detailed description of the M. tuberculosis proteome by using advanced bioinformatic procedures to glean structural and functional information about some of the components whose biological roles were unknown or obscure. Novel bioinformatics routines have been used to provide a more detailed definition of the proteome of Mycobacterium tuberculosis. About 10 genes which are mainly responsible in causing the disease were taken from Gene card database. From the computational studies like multiple sequence alignment using clustal W tool the protein sequence of TRIM gene family is related to each other. These genes were then studied separately further the domain analysis was carried out from which the common domains in the family were selected for motif studies. The conserved patterns present in gene family were obtained from clustal W tool. These conserved patterns with similar motifs showed mutation at certain places which were mainly involved in causing the disease. Protein similarity analysis, coupled with inspection of the genetic neighbourhood, was used to explore possible functional relatedness. For the faster analysis of domains and motifs the data of domains were taken in Bioperl programming. Bioperl provides access to data stores such as Gen Bank and Swiss Prot via a flexible series of sequence input/output modules, and to the emerging common sequence data storage format of the Open Bioinformatics Database Access project.
Devanga Community is one of the most significant among the profession oriented communities of Ind... more Devanga Community is one of the most significant among the profession oriented communities of India. In the preindependence days, the community was mainly confined to rural areas and was catering only to the needs of the wealthy individuals. Along with another sister sect of people constituting the Padmasalis, Devangas were involved in the procurement of cotton from agriculturists, and yarn spinning and cloth weaving from times immemorial. They give immense respect to the consanguineous marriages. This gave sample opportunity to work on blood groups of Devanga people. The human blood groups occupy a special place in Medical Genetics because of their contributions to the development of genetic principles, clinical importance in blood transfusion, and obstetrics. Blood groups have also useful applications in family studies, linkage analysis and population genetics. In the present study 220 individuals consisting of 50 families from Devanga caste were tested for Blood grouping and inheritance to understand the nature and distribution of these simple genetic characters in a selected population.
The study is to analyse computationally, the Lipid Bilayer Nano-Particles or liposomes mediated, ... more The study is to analyse computationally, the Lipid Bilayer Nano-Particles or liposomes mediated, targeted delivery of Ribavirin, a drug used primarily in the treatment of Cholangiocarcinoma, a Hepatomal disease mainly caused due to infection by liver flukes or mutation in epithelial cells. For In-Silico binding studies, 5 different liposomes are selected and their properties are studied. Based on the feasibility of the drug-liposome binding that is examined by using FEM Analysis and also those that meet the criteria for targeted drug delivery are chosen and the binding efficiency with the drug and the lipid nanoparticles are determined. It was seen that the best drug binding affinity is shown for 1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC) liposome capsulated drug with liver receptors. Then Liver Receptors, specific to cholesterol metabolism chosen for Binding studies with the above mentioned liposome were docked using LigandFit and screened primarily through dock scores using Ligscore module in Discovery Studio. The study revealed that SREBP-1 Liver Receptor has a greater efficiency in binding with the drug-liposome complex and hence can be adjudged to be most suited for targeted drug delivery experiments.
The production of alkaline protease by Bacillus licheniformis (MTCC 1483) and Bacillus subtilis o... more The production of alkaline protease by Bacillus licheniformis (MTCC 1483) and Bacillus subtilis of indigenous isolates using cheapest sources-groundnut cake (N 2 Source) and wheat bran (C source) was studied. The proteolytic activity was found maximum at 72 hr of the culture for both the bacterial types but the pH of the medium for maximal enzyme production varied as 9 for Bacillus licheniformis and as 8 for Bacillus subtilis. Thus investigations of this study reveal the possibility of employing cheapest nutrient source for microbial proteases that can be used on a commercial scale.
India is well known for its rich biological diversity. It is endowed with the rich wealth of flor... more India is well known for its rich biological diversity. It is endowed with the rich wealth of flora and fauna. Many plants contain medicinal properties, which helps to cure the diseases which have a severe impact on mankind. Nearly, 3000 medicinal plants were recognized commercially in India. In the present study, the phytochemical properties and the medicinal values of important 2 plants and 3 trees available in India are compared. The significant presence of compounds such as flavonoids, tannins, saponins, alkaloids, glycosides etc present in various sources of aqueous and methanolic extracts like Boerhaavia diffusa, Emblica officinalis, Terminalia chebula, Terminalia bellirica and, Withania sominifera were found by phytochemical screening, which accounts for its various medicinal properties.
— Many multidrug resistant organisms emerged as a notorious problem in contemporary medicine heal... more — Many multidrug resistant organisms emerged as a notorious problem in contemporary medicine healthcare. Most of the Methicillin Resistant Staphylococcus aureus (MRSA) strains are ubiquitous worldwide and are found to be Multi-Drug Resistant (MDR) due to acquisition of mecA gene encoding Penicillin Binding Protein 2A (PBP2A). The inhibitory activity or interaction of flavonoids like Galangin, Catechin, Luteolin, Quercetin and alkaloid Mimosine from Mimosa pudica against PBP2A receptors of MRSA using molecular docking were studied. Physical properties, amino acid (AA) interactions and drug likeliness of plant molecules were also analyzed. Docking results of all the selected phytoligands were compared with standard antibiotic Linezolid to identify the best leads inhibiting PBP2A of MRSA. Among those selected ligands Mimosine exhibit maximum Docking Score of 115.26 followed by Quercetin (62.95), Luteolin (58.54), Catechin (57.6) and Galangin (51.33) when compared to Linezolid (38.74). Further invitro experimental analysis will be carried out to elucidate the antimicrobial activity of selected lead molecules against MRSA strains.
Liver diseases are the second most predominant diseases next to diabetes. Treatment of most of th... more Liver diseases are the second most predominant diseases next to diabetes. Treatment of most of the liver diseases involves liver transplantation, which is not feasible due to lack of sufficient donors. Most of the drugs prescribed are random and doesn't involve proper action in the patient. Hence targeted delivery of the drug is the most viable and affordable solution for treating liver diseases. Despite many available carriers like viral vectors, metallic vectors etc, liposomes are the most suitable drug carrier molecules and they are more advantageous than other materials. Targeted delivery experiments involve much tedious experimental procedures and complex biological pathway study. Developing and analysing these nano scale compounds are also much difficult and hence for the ease of work, tools of Bioinformatics are used to analyse and interpret the results. Hence in-Silico analysis are commonly used for analysing their pathway before carrying out the in-Vitro and in-Vivo experiments. This review article emphasis on the various procedures and software used in targeted delivery experiments.
The Present Study is to analyse the targeted delivery of Ursodiol, a drug used in the treatment o... more The Present Study is to analyse the targeted delivery of Ursodiol, a drug used in the treatment of Primary Sclerosing Cholangitis (PSC) using Lipid Bilayer Nano-Particles or Solid Lipid Nano Particles(SLNPs) which is taken for insilico binding studies with various 4 different liver receptors. 3 Solid Lipid Nano Particles are chosen and Binding Efficiency of the Drug with Solid Lipid Nano Particles are Studied Using DFT Analysis and FEM Analysis. It was seen that the complete drug binding is seen in Small Unilamellar Vessicle (SUV). Chosen Liver Receptors with Ursodiol of the SUV are checked for binding studies using AutoDock 4. The Study revealed that Liver X Receptor α has a greater efficiency in binding with the Solid Lipid Nano Particles.
The study is to analyse computationally, the Lipid Bilayer Nano-Particles or liposomes mediated, ... more The study is to analyse computationally, the Lipid Bilayer Nano-Particles or liposomes mediated, targeted delivery of Ribavirin, a drug used primarily in the treatment of Cholangiocarcinoma, a Hepatomal disease mainly caused due to infection by liver flukes or by mutation in epithelial cells. For In-Silico binding studies, 5 different liposomes are selected and their properties are studied. Based on the feasibility of the drug-liposome binding that is examined by using MD Simulations, those that meet the criteria for targeted drug delivery are chosen and the binding efficiency with the drug and the lipid nanoparticles are determined. It was seen that the best drug binding affinity is shown for 1, 2 -Distearoyl-sn -glycero-3-phosphocholine (DSPC) liposome. Then Liver Receptors, specific to cholesterol and sterol metabolism are chosen for Binding studies with the above mentioned liposome (capsulated with the drug) docked using LigandFit and screened primarily through dock scores using Ligscore module in Discovery Studio. The study revealed that SREBP-1 Liver Receptor has a greater efficiency in binding with the drugliposome complex and hence can be adjudged to be most suited for targeted drug delivery experiments. W WO OR RL LD D J JO OU UR RN NA AL L O OF F P PH HA AR RM MA AC CY Y A AN ND D P PH HA AR RM MA AC CE EU UT TI IC CA AL L S SC CI IE EN NC CE ES S V Vo ol lu um me e 2 2, , I Is ss su ue e 6 6, , X XX XX X--X XX XX X. . R Re es se ea ar rc ch h A Ar rt ti ic cl le e I
The objective of this project is to apply data analysis techniques to analyze data on periodontal... more The objective of this project is to apply data analysis techniques to analyze data on periodontal disease and discover the factors that were most related to periodontal disease. In This Project a12-month, double-blind, randomized, placebocontrolled clinical study was conducted to determine the efficacy of an active drug on treating periodontal disease.
Derivatives of Indole-type compounds constitute an important class of compounds for new drug deve... more Derivatives of Indole-type compounds constitute an important class of compounds for new drug development in order to discover an effective compound against multi-drug-resistant microbial infections. Experimental studies have shown the antimicrobial analysis of oxindole derivative compounds with clinical bacterial pathogens of humans like Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Proteus mirabilis, Salmonella typhimurium, Salmonella typhi, Salmonella paratyphi 'A', Shigella sonnei and Vibrio cholera. The current study investigates the MICs of six Oxindole derivatives namely 3and 1-N-allyl-3,3-dinaphthyl-oxindole compounds with the eleven bacterial pathogens. The MIC's of six different oxindole compounds are compared with standard antibiotic like Ampicillin dissolved in various concentrations of 100, 150 & 200 µg/ml in Dimethyl Sulfoxide (DMSO). The Oxindole derivative compounds are found to show potent activity against most of the pathogens tested. Most of the derivatives were especially effective against P.aeruginosa, S.typhimurium, E.feacalis and Klebsiella pneumonia. Hence, further research on indole compounds and their derivatives could be studied out for better understanding of their antimicrobial activity using laboratory and computational tools.
Accumulation of excess fat on the body is called as "obesity". A certain amount of body fat is ne... more Accumulation of excess fat on the body is called as "obesity". A certain amount of body fat is necessary for strong energy, heat insulation, shock absorption and other functions. The normal amount of body fat is between 25-30 % in women and 18-23 % in men. Women with over 30% body fat and men with over 25% fat are considered obese. The calculation of body mass index (BMI) has also been used in the definition of obesity. The BMI equals a person's weight in kilograms (Kg) divided by their height in meters (m) squared. Since BMI describes body weight relative to height, it is strongly correlated with total body fat content in adults. Obesity is defined as having a body mass index of greater than 30.Lipoprotein lipase is an enzyme that hydrolyses lipids into two free fatty acids and one mono acyl glycerol molecule. The accumulation of this fat leads to obesity. So lipoprotein lipase plays an important role in obesity. So lipoprotein lipase used as a target protein for obesity. In our project to inhibit lipoprotein lipase activity we used drugs from plant and chemical sources. One drug is derived from drug bank (chemical source) and the other two drugs are derived from pubchem (herbal drugs). We compared and analyzed the activity of these drugs on lipoprotein lipase activity and we found the drug which best inhibits the lipoprotein lipase activity.The procedure includes following steps. They are as follows: Identification of target protein, Prediction of target structure, identification of ligands, docking of target with the ligands,finding the best drug which inhibits the target protein.
The fres h water field crab, Spiralothelphusa hydrodroma is an important human food source in par... more The fres h water field crab, Spiralothelphusa hydrodroma is an important human food source in parts of South In d ia and the crab is cons tantly exposed to pes ticides , which are us ed extens ively to control ag ricu ltu ral pes ts . Evalu atio n o f th e to xic effect o f cy p ermeth rin o n th e exp erimen tal crab fo r th e LC50 v alu e was carried o u t. Effect o f cy p ermeth rin o n th e b io ch emical ch an g es in th e n eu ro s ecreto ry cells s u ch as b rain , thoracic ganglia and eyestalk was observed. Quantitative study of biochemical changes of lactate hydrogenase (LDH), succinate dehydrogenase (SDH), acid phosphatase (ACP) and alkaline phosphatase (ALP) in th e neuros ecretory cells was undertaken.
This paper presents the details of analytical studies carried out by using advanced bioinformatic... more This paper presents the details of analytical studies carried out by using advanced bioinformatics tools on classifying all proteins which are involved in causing dormancy in human & Spermophilus tridecemlineatus a selected animal (Squirrel) and analyzing their structures, keeping their active domains in major focus. The study primarily focused on (i) Identifying genes which are related to dormancy (ii) modeling PDK4 from different organisms (iii) structural analysis to find out the structural similarity and domain details and (iv) PDK4 pathway studies to give a detailed insight into mechanism of action of PDK4. From the studies, it is observed from the studies that squirrel PDK4 shows dormancy as natural adaptation compared to human PDK4. Typical case study of Ramachandran plot of human and squirrel PDK4 has been explained in detail. RJPBCS Volume 4 Issue 1 Page No. 826
The Present Study is to analyse the targeted delivery of Ursodiol, a drug used in the treatment o... more The Present Study is to analyse the targeted delivery of Ursodiol, a drug used in the treatment of Primary Sclerosing Cholangitis (PSC) using Lipid Bilayer Nano-Particles or Solid Lipid Nano Particles(SLNPs) which is taken for insilico binding studies with various 4 different liver receptors. 3 Solid Lipid Nano Particles are chosen and Binding Efficiency of the Drug with Solid Lipid Nano Particles are Studied Using DFT Analysis and FEM Analysis. It was seen that the complete drug binding is seen in Small Unilamellar Vessicle (SUV). Chosen Liver Receptors with Ursodiol of the SUV are checked for binding studies using AutoDock 4. The Study revealed that Liver X Receptor α has a greater efficiency in binding with the Solid Lipid Nano Particles.
The fres h water field crab, Spiralothelphusa hydrodroma is an important human food source in par... more The fres h water field crab, Spiralothelphusa hydrodroma is an important human food source in parts of South India and the crab is cons tantly expos ed to pes ticides , which are us ed extens ively to control ag ricu ltu ral p es ts . Ev alu atio n o f th e to xic effect o f cy p ermeth rin o n th e exp erimen tal crab fo r th e LC50 v alu e was carried out. Effect of cypermethrin on the quantitative study of nutritive value viz. protein, carbohydrate and lipid in ovary, spermatheca, hepatopancreas, muscle, g ills, haemolymph, brain, thoracic ganglia and eyestalk was observed. 50 Ke y wor ds : Protein, carbohydrate, lipid, LC , Cypermethrin, Spiralothelphusa hydrodroma
Boerhavia diffusa is a medicinal plant used in treatment of autoimmune diseases like Rheumatic ar... more Boerhavia diffusa is a medicinal plant used in treatment of autoimmune diseases like Rheumatic arthritis, Alzheimer's disease and Colon cancer. The present study investigates the application of bioinformatics tools showing binding affinity of BoeravinoneE with the human immunomodulatory receptor molecules like GMCSF and TNFα responsible for maturation and activation of dendritic cells. The binding pocket sites, the internal energy, the hydrogen bond interactions and the interacting amino acid residues of the human immunomodulatory protein molecules with BoeravinoneE were analyzed through molecular docking. Among the human immunomodulatory molecules docked, which are responsible for DCs maturation and activation, BoeravinoneE was binding with receptors of GMCSF, and TNFα exhibiting a maximum Dock score of 47.43, 59.31 and internal binding energy of 5.5 J/mol, 0.10 J/mol. BoeravinoneE was docked with binding site aminoacids, LEU246 of GMCSFR and SER74, ASN110 of TNF-αR as predicted from UNIPROT and PDBSum. This present study could also be performed further for checking immunomodulation activity studies in invitro and invivo in mice.
The aim of the paper is to provide a detailed description of the M. tuberculosis proteome by usin... more The aim of the paper is to provide a detailed description of the M. tuberculosis proteome by using advanced bioinformatic procedures to glean structural and functional information about some of the components whose biological roles were unknown or obscure. Novel bioinformatics routines have been used to provide a more detailed definition of the proteome of Mycobacterium tuberculosis. About 10 genes which are mainly responsible in causing the disease were taken from Gene card database. From the computational studies like multiple sequence alignment using clustal W tool the protein sequence of TRIM gene family is related to each other. These genes were then studied separately further the domain analysis was carried out from which the common domains in the family were selected for motif studies. The conserved patterns present in gene family were obtained from clustal W tool. These conserved patterns with similar motifs showed mutation at certain places which were mainly involved in causing the disease. Protein similarity analysis, coupled with inspection of the genetic neighbourhood, was used to explore possible functional relatedness. For the faster analysis of domains and motifs the data of domains were taken in Bioperl programming. Bioperl provides access to data stores such as Gen Bank and Swiss Prot via a flexible series of sequence input/output modules, and to the emerging common sequence data storage format of the Open Bioinformatics Database Access project.
Devanga Community is one of the most significant among the profession oriented communities of Ind... more Devanga Community is one of the most significant among the profession oriented communities of India. In the preindependence days, the community was mainly confined to rural areas and was catering only to the needs of the wealthy individuals. Along with another sister sect of people constituting the Padmasalis, Devangas were involved in the procurement of cotton from agriculturists, and yarn spinning and cloth weaving from times immemorial. They give immense respect to the consanguineous marriages. This gave sample opportunity to work on blood groups of Devanga people. The human blood groups occupy a special place in Medical Genetics because of their contributions to the development of genetic principles, clinical importance in blood transfusion, and obstetrics. Blood groups have also useful applications in family studies, linkage analysis and population genetics. In the present study 220 individuals consisting of 50 families from Devanga caste were tested for Blood grouping and inheritance to understand the nature and distribution of these simple genetic characters in a selected population.
The study is to analyse computationally, the Lipid Bilayer Nano-Particles or liposomes mediated, ... more The study is to analyse computationally, the Lipid Bilayer Nano-Particles or liposomes mediated, targeted delivery of Ribavirin, a drug used primarily in the treatment of Cholangiocarcinoma, a Hepatomal disease mainly caused due to infection by liver flukes or mutation in epithelial cells. For In-Silico binding studies, 5 different liposomes are selected and their properties are studied. Based on the feasibility of the drug-liposome binding that is examined by using FEM Analysis and also those that meet the criteria for targeted drug delivery are chosen and the binding efficiency with the drug and the lipid nanoparticles are determined. It was seen that the best drug binding affinity is shown for 1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC) liposome capsulated drug with liver receptors. Then Liver Receptors, specific to cholesterol metabolism chosen for Binding studies with the above mentioned liposome were docked using LigandFit and screened primarily through dock scores using Ligscore module in Discovery Studio. The study revealed that SREBP-1 Liver Receptor has a greater efficiency in binding with the drug-liposome complex and hence can be adjudged to be most suited for targeted drug delivery experiments.
The production of alkaline protease by Bacillus licheniformis (MTCC 1483) and Bacillus subtilis o... more The production of alkaline protease by Bacillus licheniformis (MTCC 1483) and Bacillus subtilis of indigenous isolates using cheapest sources-groundnut cake (N 2 Source) and wheat bran (C source) was studied. The proteolytic activity was found maximum at 72 hr of the culture for both the bacterial types but the pH of the medium for maximal enzyme production varied as 9 for Bacillus licheniformis and as 8 for Bacillus subtilis. Thus investigations of this study reveal the possibility of employing cheapest nutrient source for microbial proteases that can be used on a commercial scale.
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