ObjectiveEvaluate efficacy of platelet-derived lyophilized growth factors (L-GF) injection versus... more ObjectiveEvaluate efficacy of platelet-derived lyophilized growth factors (L-GF) injection versus placebo in treatment of subacromial impingement (SIS).MethodsSixty patients with SIS (clinically and sonographically) were included in this randomized double blind placebo controlled study. Patients were randomly divided to receive either saline (group 1) or L-GF (group 2) injection, under US-guidance. Clinical examination, Visual analogue scale of pain, Shoulder Pain and Disability Index (SPADI), and shoulder ultrasound were done before and at 8th week after injection. ResultsFollow up assessment showed statistically significant improvement in L-GF group as regards active flexion & active and passive internal rotation and extension, SPADI-Disability scale, VAS of pain as well as thickness of the supraspinatus tendon by US. Concerning painful arc, regression analysis has shown that placebo group is about 30 times more than the L-GF group to get painful arc at follow up. On the other han...
Background: The antineoplastic agent Cyclophosphamide (CP) induces reproductive toxicity. New str... more Background: The antineoplastic agent Cyclophosphamide (CP) induces reproductive toxicity. New strategies for protecting ovarian tissue damage in women with chemotherapy-induced reproductive toxicity are essential. This study was designed to evaluate the possible protective effect of combined treatment with L-GFequina on CP-induced reproductive toxicity in the mature female rat. Methodology: Forty mature female rats were assigned into four groups: First group, control: rats were intraperitoneally injected (IP) with 200 μl sterile saline solution on days 1 and 10; Group 2 (CP): were IP injected with 75 mg/kg on days 1 and 10 to induce POI); Group 3 (CP + L-GFequina): as in group 2 + IP injected with 200 μl rehydrated L-GFequina half-hour after CP injection on day 1 and 10); Group 4 (L-GFequina): rats were IP injected with 200 μl L-GFequina on day 1 and 10). Blood samples were collected for complete blood picture and determinations of nitric oxide and malondialdehyde. Animals were sacr...
The purpose of this study was to determine the impact of intraovarian injections of a reconstitut... more The purpose of this study was to determine the impact of intraovarian injections of a reconstituted lyophilized growth-promoting factor extracted from horse blood platelets (L-GFequina) on the number of ovarian follicles, the recovery of cumulus–oocyte complexes (COCs), and embryo development to the blastocyst stage in Holstein cows. Thus, 12 Holstein cows were assigned to three protocols. According to the number of punctured follicles in protocol 1, ovum pick-up (OPU) was conducted on days 6 and 14 of the cycle (day 0 = estrus). In protocol 2, every large follicle (more than 7 mm) was removed, and 1 mL of L-GFequina was intraovarian injected (day 0). Two days later, equine chorionic gonadotropin (eCG) was administered, and OPU sessions were conducted on days 6, 10, and 14. The same ovarian stimulation procedure as that in protocol 2 was performed in protocol 3, except that equine L-GFequina was not supplied. OPU was carried out on days 6 and 10 of the cycle. The results indicate th...
Most cancer were diagnosed by using a few simple stains and looking at the appearance of cells un... more Most cancer were diagnosed by using a few simple stains and looking at the appearance of cells under the microscope. But there were only a few types of lymphoma cancer that could easily be distinguished in this way, and it often appeared that tumors of the same cell type behaved very differently in different individuals. It was obvious that something else was going on at the molecular level to account for these differences. As medical diagnosis progressed to the study of molecules, new techniques such as flow cytometry were devised to identify specific molecules, located on the surface of white blood cells (CD markers). In this study was carried out to assess the relation between lymphoma stem cells and 3 from specific CD markers which are CD 19, CD23 and CD 24by using flowcytometry technique to help diagnose lymphoma cancer. Conclusively, in this study, CD 24 marker showed high positivity in Hodgkin lymphoma disease, and can be used as a diagnostic marker in this type of haematolog...
Different studies had evaluated the efficacy of fractional CO2 laser and platelet‐rich plasma (PR... more Different studies had evaluated the efficacy of fractional CO2 laser and platelet‐rich plasma (PRP) in treating striae distensae (SD). Lyophilized growth factors (LGF) represent another form of delivering growth factors similar to PRP with a more standardized method. To the best of our knowledge, no previous trials have been reported using LGF in SD.
This study was designed to examine the effect of the intrauterine infusion of platelet-rich plasm... more This study was designed to examine the effect of the intrauterine infusion of platelet-rich plasma (PRP) or equine lyophilized growth factor (L-GFequina) on the follicular growth, endometrial thickness, estrus cycle length, and pregnancy rate in purebred Arabian mares. A total of 73 purebred Arabian mares who experienced repeat breeding for three successive cycles were randomly divided into the following three groups: control group, without treatment; second Group (PRP group), in which mares were intrauterine infused with 20 mL of fresh PRP on the second day after the end of physic estrus phase; and the third group (L-GFequina Group), consisting of mares that were intrauterine infused with 20 mL of reconstituted lyophilized horse platelets growth factors (L-GFequina) on the second day after the estrus phase. The results showed no significant difference between control and treated groups in the diameter of the preovulatory follicles during the post treatment cycle. The endometrium th...
Background: Using intra-articular injections of autologous platelet-rich plasma (PRP) has showen ... more Background: Using intra-articular injections of autologous platelet-rich plasma (PRP) has showen promising results in treatment of knee osteoarthritis (KOA). Its regenerative properties depends on the amount of growth factors (GF) released after platelets activation. Using large-volume of blood for obtaining allogenic PRP can provide higher concentrations of platelet and GF. However once prepared, PRP is stable for only 8-hours. Freeze drying (lyophilization) can be used to stabilize the biological materials for prolonged storage without causing their damage (1). Objectives: Investigating the effectiveness of intra-articular allogenic lyophilized growth factors (L-GF) injection in patients with symptomatic KOA using clinical and knee ultrasonography assessment. Methods: 30-patients with symtomatic primary KOA, diagnosed according to revised criteria of the ACR (2), were enrolled. Local ethical approval was obtained. All patients had given a written informed consent. The study group was equally randomized into intervention and control groups. Both was subjected to baseline functional assessment using WOMAC score; evaluating pain, stiffness, and physical function (3), and ultrasonography (US) assessment of knee effusion. The patients in control group were kept on their medications without intervention. The intervention group was recieved two doses of L-GF; at baseline and after 2-months. L-GF was prepared from platelets derived from individual whole blood donations. Each unit of platelets was tested in and found to be non-reactive for HBsAg, HIV I & II antibodies, HIV p-24 antigen, HCV antibodies and antibodies to Treponema Palidum, by licensed assay methods. Seronegative plasma were further examined by Nuclear Acid Testing. Further viral inactivation by UV-radiation and Riboflavin by the Mirasol system was done. The platelets in the buffy coat layer were activated using CaCl 2 to release their GF. Excess water, cellular elements and fibrinogen were removed and the remaining GF were "ultraconcentrated." Lyophilization of the obtained GF was then processed. The L-GF was supplied as powder in tightly sealed container and was stored at 2°to 8°C. Prior to use, reconstitution of the product was done using 1-ml saline and 1-ml lignocaine then the mixture was kept at ambient temperature for 5-min. to ensure complete protein re-hydration. After 6-months the WOMAC score and knee US were repeated for all particepients. Then percent of improvement of WOMAC and US detected effusion were calculated for all patients. Results: The patients in the intervention group was significantly older (56 ±9 years) and had heigher BMI (38±5.3 kg/m 2) in comparison to control (44±11 years, 33±5.6 kg/m 2). Both groups showed statistically insignificant difference regarding the baseline total WOMAC score and radiographic assessment. Post injection pain was reported by all patients in intervention group, it lasted only for 2.4±0.83 hours and was graded as mild. The mean of total WOMAC score and its 3-compenents in addition to knee effusion were decreased significantly in the intervention group after 2 months and 6 months of follow-up (figuer-A). After 6-months, the intervention group showed statistically significant persentage of improvement regarding WOMAC scores and effusion in comparison to the control (figuer-B). Conclusion: Allogenic L-GF was well tolerated and showed encouraging results in patients with symptomatic KOA, regarding improvement of pain, stiffness and function in addition to decreasing knee effsuion.
ObjectiveEvaluate efficacy of platelet-derived lyophilized growth factors (L-GF) injection versus... more ObjectiveEvaluate efficacy of platelet-derived lyophilized growth factors (L-GF) injection versus placebo in treatment of subacromial impingement (SIS).MethodsSixty patients with SIS (clinically and sonographically) were included in this randomized double blind placebo controlled study. Patients were randomly divided to receive either saline (group 1) or L-GF (group 2) injection, under US-guidance. Clinical examination, Visual analogue scale of pain, Shoulder Pain and Disability Index (SPADI), and shoulder ultrasound were done before and at 8th week after injection. ResultsFollow up assessment showed statistically significant improvement in L-GF group as regards active flexion & active and passive internal rotation and extension, SPADI-Disability scale, VAS of pain as well as thickness of the supraspinatus tendon by US. Concerning painful arc, regression analysis has shown that placebo group is about 30 times more than the L-GF group to get painful arc at follow up. On the other han...
Background: The antineoplastic agent Cyclophosphamide (CP) induces reproductive toxicity. New str... more Background: The antineoplastic agent Cyclophosphamide (CP) induces reproductive toxicity. New strategies for protecting ovarian tissue damage in women with chemotherapy-induced reproductive toxicity are essential. This study was designed to evaluate the possible protective effect of combined treatment with L-GFequina on CP-induced reproductive toxicity in the mature female rat. Methodology: Forty mature female rats were assigned into four groups: First group, control: rats were intraperitoneally injected (IP) with 200 μl sterile saline solution on days 1 and 10; Group 2 (CP): were IP injected with 75 mg/kg on days 1 and 10 to induce POI); Group 3 (CP + L-GFequina): as in group 2 + IP injected with 200 μl rehydrated L-GFequina half-hour after CP injection on day 1 and 10); Group 4 (L-GFequina): rats were IP injected with 200 μl L-GFequina on day 1 and 10). Blood samples were collected for complete blood picture and determinations of nitric oxide and malondialdehyde. Animals were sacr...
The purpose of this study was to determine the impact of intraovarian injections of a reconstitut... more The purpose of this study was to determine the impact of intraovarian injections of a reconstituted lyophilized growth-promoting factor extracted from horse blood platelets (L-GFequina) on the number of ovarian follicles, the recovery of cumulus–oocyte complexes (COCs), and embryo development to the blastocyst stage in Holstein cows. Thus, 12 Holstein cows were assigned to three protocols. According to the number of punctured follicles in protocol 1, ovum pick-up (OPU) was conducted on days 6 and 14 of the cycle (day 0 = estrus). In protocol 2, every large follicle (more than 7 mm) was removed, and 1 mL of L-GFequina was intraovarian injected (day 0). Two days later, equine chorionic gonadotropin (eCG) was administered, and OPU sessions were conducted on days 6, 10, and 14. The same ovarian stimulation procedure as that in protocol 2 was performed in protocol 3, except that equine L-GFequina was not supplied. OPU was carried out on days 6 and 10 of the cycle. The results indicate th...
Most cancer were diagnosed by using a few simple stains and looking at the appearance of cells un... more Most cancer were diagnosed by using a few simple stains and looking at the appearance of cells under the microscope. But there were only a few types of lymphoma cancer that could easily be distinguished in this way, and it often appeared that tumors of the same cell type behaved very differently in different individuals. It was obvious that something else was going on at the molecular level to account for these differences. As medical diagnosis progressed to the study of molecules, new techniques such as flow cytometry were devised to identify specific molecules, located on the surface of white blood cells (CD markers). In this study was carried out to assess the relation between lymphoma stem cells and 3 from specific CD markers which are CD 19, CD23 and CD 24by using flowcytometry technique to help diagnose lymphoma cancer. Conclusively, in this study, CD 24 marker showed high positivity in Hodgkin lymphoma disease, and can be used as a diagnostic marker in this type of haematolog...
Different studies had evaluated the efficacy of fractional CO2 laser and platelet‐rich plasma (PR... more Different studies had evaluated the efficacy of fractional CO2 laser and platelet‐rich plasma (PRP) in treating striae distensae (SD). Lyophilized growth factors (LGF) represent another form of delivering growth factors similar to PRP with a more standardized method. To the best of our knowledge, no previous trials have been reported using LGF in SD.
This study was designed to examine the effect of the intrauterine infusion of platelet-rich plasm... more This study was designed to examine the effect of the intrauterine infusion of platelet-rich plasma (PRP) or equine lyophilized growth factor (L-GFequina) on the follicular growth, endometrial thickness, estrus cycle length, and pregnancy rate in purebred Arabian mares. A total of 73 purebred Arabian mares who experienced repeat breeding for three successive cycles were randomly divided into the following three groups: control group, without treatment; second Group (PRP group), in which mares were intrauterine infused with 20 mL of fresh PRP on the second day after the end of physic estrus phase; and the third group (L-GFequina Group), consisting of mares that were intrauterine infused with 20 mL of reconstituted lyophilized horse platelets growth factors (L-GFequina) on the second day after the estrus phase. The results showed no significant difference between control and treated groups in the diameter of the preovulatory follicles during the post treatment cycle. The endometrium th...
Background: Using intra-articular injections of autologous platelet-rich plasma (PRP) has showen ... more Background: Using intra-articular injections of autologous platelet-rich plasma (PRP) has showen promising results in treatment of knee osteoarthritis (KOA). Its regenerative properties depends on the amount of growth factors (GF) released after platelets activation. Using large-volume of blood for obtaining allogenic PRP can provide higher concentrations of platelet and GF. However once prepared, PRP is stable for only 8-hours. Freeze drying (lyophilization) can be used to stabilize the biological materials for prolonged storage without causing their damage (1). Objectives: Investigating the effectiveness of intra-articular allogenic lyophilized growth factors (L-GF) injection in patients with symptomatic KOA using clinical and knee ultrasonography assessment. Methods: 30-patients with symtomatic primary KOA, diagnosed according to revised criteria of the ACR (2), were enrolled. Local ethical approval was obtained. All patients had given a written informed consent. The study group was equally randomized into intervention and control groups. Both was subjected to baseline functional assessment using WOMAC score; evaluating pain, stiffness, and physical function (3), and ultrasonography (US) assessment of knee effusion. The patients in control group were kept on their medications without intervention. The intervention group was recieved two doses of L-GF; at baseline and after 2-months. L-GF was prepared from platelets derived from individual whole blood donations. Each unit of platelets was tested in and found to be non-reactive for HBsAg, HIV I & II antibodies, HIV p-24 antigen, HCV antibodies and antibodies to Treponema Palidum, by licensed assay methods. Seronegative plasma were further examined by Nuclear Acid Testing. Further viral inactivation by UV-radiation and Riboflavin by the Mirasol system was done. The platelets in the buffy coat layer were activated using CaCl 2 to release their GF. Excess water, cellular elements and fibrinogen were removed and the remaining GF were "ultraconcentrated." Lyophilization of the obtained GF was then processed. The L-GF was supplied as powder in tightly sealed container and was stored at 2°to 8°C. Prior to use, reconstitution of the product was done using 1-ml saline and 1-ml lignocaine then the mixture was kept at ambient temperature for 5-min. to ensure complete protein re-hydration. After 6-months the WOMAC score and knee US were repeated for all particepients. Then percent of improvement of WOMAC and US detected effusion were calculated for all patients. Results: The patients in the intervention group was significantly older (56 ±9 years) and had heigher BMI (38±5.3 kg/m 2) in comparison to control (44±11 years, 33±5.6 kg/m 2). Both groups showed statistically insignificant difference regarding the baseline total WOMAC score and radiographic assessment. Post injection pain was reported by all patients in intervention group, it lasted only for 2.4±0.83 hours and was graded as mild. The mean of total WOMAC score and its 3-compenents in addition to knee effusion were decreased significantly in the intervention group after 2 months and 6 months of follow-up (figuer-A). After 6-months, the intervention group showed statistically significant persentage of improvement regarding WOMAC scores and effusion in comparison to the control (figuer-B). Conclusion: Allogenic L-GF was well tolerated and showed encouraging results in patients with symptomatic KOA, regarding improvement of pain, stiffness and function in addition to decreasing knee effsuion.
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Papers by Hossam Fahmy