Papers by Annahita Rezaie
Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences, 2016
The aim of this study was to develop and optimize deformable liposome for topical delivery of tre... more The aim of this study was to develop and optimize deformable liposome for topical delivery of tretinoin. Liposomal formulations were designed based on the full factorial design and prepared by fusion method. The influence of different ratio of soy phosphatidylcholine and transcutol (independent variables) on incorporation efficiency and drug release in 15 min and 24 h (responses) from liposomal formulations was evaluated. Liposomes were characterized for their vesicle size and Differential Scanning Calorimetry (DSC) was used to investigate changes in their thermal behavior. The penetration and retention of drug was determined using mouse skin. Also skin histology study was performed. Particle size of all formulations was smaller than 20 nm. Incorporation efficiency of liposomes was 79-93 %. Formulation F7 (25:5) showed maximum drug release. Optimum formulations were selected based on the contour plots resulted by statistical equations of drug release in 15 min and 24 h. Solubility p...
Veterinary Research Forum an International Quarterly Journal, 2012
An 11 year -old mixed female Labrador was presented with two masses in trunk and neck. The tumora... more An 11 year -old mixed female Labrador was presented with two masses in trunk and neck. The tumoral masses were excised and sent for histopathological and immunohistochemical analyses. Histopathological examination of masses revealed diffuse infiltration of small sized lymphoid cells in subcutaneous tissue which were intense around the blood vessels. More than 10% lymphoid cells were CD3 positive in the immunohistochemical staining and most of them were accumulated around vessels. Protein 53 (p53) expression was detected by brown nuclei in immunohistochemical staining. Subcutaneous lymphoma was diagnosed according to histopathological results. After 6 months the case was referred with multicentric lymphoma and based on the owner request euthanasia was performed. These findings emphasize on poor prognosis for tumors with p53 mutation.
Jundishapur Journal of Natural Pharmaceutical Products, Feb 1, 2014
In the recent years nanostructured materials have been the focus of researches due to their wide-... more In the recent years nanostructured materials have been the focus of researches due to their wide-spread possibilities to provide new shapes and structures for some materials. Microemulsions can provide uniform nano-sized droplets for templating. Microemulsions are isotropic, thermodynamically-stable systems of oil, water and surfactant with a 20-200 nm droplet size. They can be prepared as oil-in-water (o/w), water-in-oil (w/o) or bicontinuous systems, depending on the equilibrium spontaneous curvature of the surfactant layer at the oil-water interface. Objectives: The aim of this study was to introduce a system designed to improve and enhance the bioavailability of bioflavonoids in the Prunus cerasus (sour cherry) seed kernel extract by developing a novel delivery system, i.e. microemulsion (nanosized particles). Materials and Methods: Microemulsion formulations were prepared by mixing appropriate amounts of surfactants (Tween 80 and Span 20), cosurfactant (propylene glycol) (3:1 ratio), and oil phase (olive oil). The prepared microemulsions were evaluated regarding their mean droplet size, transparency, viscosity, and pH. Sour cherry kernel extract microemulsion was orally administered to mice at doses of 2.5%, 5%, and 10% for 10 days. On the last day, their blood as well as their liver and kidney were used for biochemical and histopathological analyses, respectively. Results: Biochemical factors levels and the pathological study indicated that there were not significant differences in microemulsion extracts compared with the control group (P > 0.05). Conclusions: Not only no toxicity evidence of this product was observed in the dose range used in foods or healthcare, but also it improved the cardiac function recovery.
Iranian Journal of Endocrinology and Metabolism
Introduction: Impaired wound healing in diabetic patients is a major clinical problem, which is a... more Introduction: Impaired wound healing in diabetic patients is a major clinical problem, which is associated with
Indian Journal of Pharmacology, 2015
Objectives: Ellagic acid (EA) has shown antinociceptive and anti-infl ammatory effects. Inducible... more Objectives: Ellagic acid (EA) has shown antinociceptive and anti-infl ammatory effects. Inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2) enzymes and also cytokines play a key role in many infl ammatory conditions. This study was aimed to investigate the mechanisms involved in the anti-infl ammatory effect of EA. Materials and Methods: Carrageenan-induced mouse paw edema model was used for induction of infl ammation.
Jundishapur journal of natural pharmaceutical products, 2015
The development of drug delivery systems has improved the therapeutic and toxic properties of exi... more The development of drug delivery systems has improved the therapeutic and toxic properties of existing drugs in therapy. Microemulsion systems are novel vehicles for drug delivery, which have been developed in recent years. These systems are currently of interest to the pharmaceutical scientist because of their considerable potential to act as drug delivery vehicles by incorporating into a wide range of drug molecules. Although these systems improved solubility and bioavailability of drugs, they may have potential toxic effects on the body organs. The purpose of this study was to examine a possible hepatotoxic and nephrotoxic effect of lithium carbonate microemulsion (LCME) in a mice model. Eighty male Swiss albino mice were randomly allocated to eight experimental groups, as follows: Group 1, as negative control group were treated orally with normal saline (0.9% NaCl); Group 2, received microemulsion base without drug as control group; Groups 3 to 5, received lithium carbonate (LC)...
Veterinary research forum : an international quarterly journal, 2012
An 11 year - old mixed female Labrador was presented with two masses in trunk and neck. The tumor... more An 11 year - old mixed female Labrador was presented with two masses in trunk and neck. The tumoral masses were excised and sent for histopathological and immunohistochemical analyses. Histopathological examination of masses revealed diffuse infiltration of small sized lymphoid cells in subcutaneous tissue which were intense around the blood vessels. More than 10% lymphoid cells were CD3 positive in the immunohistochemical staining and most of them were accumulated around vessels. Protein 53 (p53) expression was detected by brown nuclei in immunohistochemical staining. Subcutaneous lymphoma was diagnosed according to histopathological results. After 6 months the case was referred with multicentric lymphoma and based on the owner request euthanasia was performed. These findings emphasize on poor prognosis for tumors with p53 mutation.
Jundishapur journal of natural pharmaceutical products, 2014
In the recent years nanostructured materials have been the focus of researches due to their wide-... more In the recent years nanostructured materials have been the focus of researches due to their wide-spread possibilities to provide new shapes and structures for some materials. Microemulsions can provide uniform nano-sized droplets for templating. Microemulsions are isotropic, thermodynamically-stable systems of oil, water and surfactant with a 20-200 nm droplet size. They can be prepared as oil-in-water (o/w), water-in-oil (w/o) or bicontinuous systems, depending on the equilibrium spontaneous curvature of the surfactant layer at the oil-water interface. The aim of this study was to introduce a system designed to improve and enhance the bioavailability of bioflavonoids in the Prunus cerasus (sour cherry) seed kernel extract by developing a novel delivery system, i.e. microemulsion (nanosized particles). Microemulsion formulations were prepared by mixing appropriate amounts of surfactants (Tween 80 and Span 20), cosurfactant (propylene glycol) (3:1 ratio), and oil phase (olive oil). T...
Veterinary and comparative oncology, Jan 22, 2014
Mouse Double Minute-2 (MDM2) is an ubiquitin ligase which is overexpressed or its promoter polymo... more Mouse Double Minute-2 (MDM2) is an ubiquitin ligase which is overexpressed or its promoter polymorphism has been reported in different tumours. The objective of this study was to examine the MDM2 protein expression and its promoter polymorphism in some canine tumours. Twenty specimens were collected from 20 dogs with 15 mammary gland carcinomas, 3 lymphomas, 1 transmissible venereal tumour and 1 trichoblastoma. Samples were analysed immunohistochemically using human antibody against MDM2 protein. PCR and DNA sequencing were carried out to identify MDM2 promoter polymorphism. MDM2 gene was expressed in 13 of 20 samples including 11 mammary carcinomas, 1 lymphoma and 1 trichoblastoma. We found 94% homology between canine and human sequences. Four mutations including G169C, A177G, G291T and A177G were identified in different types of breast carcinomas. An extra p53 response element was found in a mixed mammary carcinoma.
Journal of Biological Sciences, 2009
Comparative Clinical Pathology, 2011
Canine mammary gland carcinoma is ranked second after skin tumours in dogs in terms of frequency.... more Canine mammary gland carcinoma is ranked second after skin tumours in dogs in terms of frequency. Due to certain microscopic and biological features, canine tumours are very similar to human carcinomas, which may make the dog a good animal model. The objective of this study was to describe Ki67 protein expression properties using immuno-histochemical (IHC) staining using human antibody. In this study, 30 mammary gland tumour samples were studied. The histological diagnosis was made on the basis of the current World Health Organization classification for canine mammary tumours. Tumours were then graded histologically in accordance with the Elston and Ellis method for human breast tumours. IHC staining using MIB-1 antibody for detecting expression of Ki67 proteins (pKi67), was performed on canine mammary gland carcinoma tissue. Results from the semi-quantitative IHC assessment of pKi67 showed that 20% were negative, 20% were one plus, 36.5% were two plus, 23.3% were three plus. There was a significant difference between histological grade and pKi67 expression. This method would help the pathologist and clinician in determining a more accurate prognosis and treatment in canine mammary carcinoma and facilitate comparative studies of canine and human research.
Comparative Clinical Pathology, 2012
Laboratory animals, Jan 28, 2016
It has been shown that embryonated chicken eggs can be used as animal models for experimental inf... more It has been shown that embryonated chicken eggs can be used as animal models for experimental infections. The aim of the present study was to investigate pigeon embryonated eggs as animal models for experimental neosporosis. An infection with Neospora caninum Nc1 isolate was conducted in chicken and pigeon embryonated eggs to evaluate LD50. After calculation of LD50, 2LD50 of tachyzoites were injected into the eggs. Macroscopic changes of each embryo were observed, and immunohistochemistry (IHC) and molecular methods were used to investigate the parasitic distribution in the tissues. In the present study, histopathological changes were considered, and sections of those used for histopathological examination including the heart, liver, brain and chorioallantoic (CA) membrane were also subjected to IHC. Pigeon embryos showed more macroscopic changes than chicken embryos. A hemorrhage of the CA membrane was the main gross lesion. Microscopic examination of tissues revealed acute neospo...
Journal of aquatic animal health, 2016
Rhabdomyosarcoma is a primitive neoplasm that originates from skeletal muscle progenitor cells. I... more Rhabdomyosarcoma is a primitive neoplasm that originates from skeletal muscle progenitor cells. In a routine inspection of a cyprinid farm in southwestern Iran, an approximately 2-year-old female Silver Carp was observed to have a raised mass located on the dorsolateral surface just caudal to the head. Macroscopic examination revealed a firm irregular fleshy pink mass (5 × 4 cm, depth = 3 cm) that appeared to arise from the subcutaneous musculature. Histologic sections were prepared using routine methods and separate sections were stained with hematoxylin-eosin and Massons' trichrome. Microscopically, the tumor mass was composed of spindle cells that were densely packed and arranged in long interwoven bundles. The nuclei were vesicular and oval to elongated or cigar-shaped. Nuclear pleomorphism and multinucleate tumor giant cells were clearly evident. The neoplastic cell cytoplasm was eosinophilic with indistinct cell margins, and clear cross striations were observed in fibrils....
Jundishapur Journal of Natural Pharmaceutical Products, 2016
Zahedan Journal of Research in Medical Sciences, Nov 1, 2013
Background: Evaluation of healing effects of Echinacea extract in Arsenic induced dermal necrosis... more Background: Evaluation of healing effects of Echinacea extract in Arsenic induced dermal necrosis in rat is the objective of this study.
Background: Evaluation of healing effects of Echinacea extract in Arsenic induced dermal necrosis... more Background: Evaluation of healing effects of Echinacea extract in Arsenic induced dermal necrosis in rat is the objective of this study. Materials and Methods: In this experimental study 20 male Wistar rats were divided to 2 groups. Dermal necrosis was induced by subcutaneously arsenic injection (4mg/kg) for 10 days. In group 2, after arsenic receiving, Echinacea were injected intraperitoneally (400mg/kg). After last day of injection, rats were euthanizes and pathologic samples were collected from dermal ulcers5 T . Results: Histopathologic results revealed necrosis of different dermal layers in arsenic group. There were inflammatory exudates instead of impaired structures. In group 2, there were granulation tissue with high cellularity and new vessels5 T . Conclusion: According to this research findings arsenic can induce dermal necrosis which is a good animal model for dermatologic researches and also Echinacea has healing effects and can protect and limit the Arsenic effects5 T .5 T
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Papers by Annahita Rezaie