Papers by Basma Badreldin
Medicine Updates (Online), Oct 1, 2022
Background: Venous leg ulcers (VLU) consider the commonest type of leg ulceration; venous insuffi... more Background: Venous leg ulcers (VLU) consider the commonest type of leg ulceration; venous insufficiency is the main cause of it. Intra lesion injection of Platelet-rich plasma (PRP) is a potential factor for promoting ulcer healing by the effect of growth factors (GF). The aim was to evaluate the efficacy and safety of (PRP) injection in chronic non-healing (VLU) versus conventional compression therapy. Methods: a randomized prospective comparative study, in 42 patients were managed by dividing into two equal groups; Group (1) managed by PRP injection inside the VLU after preparing the ulcer for healing, and relieving the causes of non-healing ulcer (infection and necrotic tissues or venous insufficiency). Group (2) managed the ulcers by conventional compression therapy. All patients were assisted for 3, 6,9 and 12 months or until wound healing. The injection was performed weekly and assessed granulation tissue healing. Results: The mean percentage of wound closed of PRF injection group was discovered significantly higher than conventional group. With statistically difference in rate of healing ulcer between the PRF group and control group. The mean of reduction in ulcer area in group (1) within 12 months was 1.2 + 0.5, and in group (2) was 3.9 + 1.6. the rate of improvement was 92.7% and 77.1% respectively (P value <0.05). Conclusions: Injection of PRP inside chronic VLU showed better clinical outcomes in comparing with conventional compression therapy alone.
Egyptian Journal of Bronchology, 2015
Journal of Blood Transfusion, Aug 2, 2015
Platelet rich plasma (PRP) is hemoconcentration with platelets concentration above baseline value... more Platelet rich plasma (PRP) is hemoconcentration with platelets concentration above baseline values and high concentration of many growth factors. The aim of this study was to assess freezing effect on vascular endothelial growth factor (VEGF) release from PRP using two different activation methods to simplify its use in different clinical applications. PRP was prepared using two-centrifugation steps method from 12 qualified blood donors. VEGF concentrations were measured in fresh PRP and after freezing/thawing for one and three weeks with two methods of activation using (i) calcium gluconate and (ii) calcium gluconate and thrombin. Platelets count was significantly increased compared to baseline whole blood values in all fresh and frozen PRP samples (p value was <0.05). No significant difference was found between VEGF concentrations after activating fresh and frozenthawed PRP samples for one and three weeks by calcium alone or calcium with thrombin, and also no significant difference was found when freezing period was extended from one to three weeks. Our results showed that platelets count does not correlate with variable levels of VEGF. PRP could be prepared once and preserved frozen for at least three weeks for the next treatment sessions and activation with thrombin addition to calcium will not augment the growth factor release.
Current Cancer Therapy Reviews, Jan 17, 2023
Hepatocellular carcinoma is a common morbid condition in our country, due to various reason, howe... more Hepatocellular carcinoma is a common morbid condition in our country, due to various reason, however the current surveillance protocols which depend on Alfa-fetoprotein and ultrasound could not predict early cases in many situations. Aim: To evaluate the pattern of serum interleukin-10 in patients with cirrhotic liver versus patients with hepatocellular carcinoma. Methods: We compared the level of Interleukin-10 in 44 patients with liver cirrhosis versus 36 patients with HCC. Results: patients with HCC have significantly higher IL-10 than cirrhotic group (10.86 ± 4.48, 7.005 ± 6.1) respectively (p = 0.002*). Our results also shows that the best cut off value of IL-10 for detection of HCC among HCV patients is > 7.5 with sensitivity of 86% and specificity of 75% (p = 0.0001*); IL-10 was significantly negatively correlated with albumin (p = 0.003*), and positively correlated to WBC count (p = 0.001*). Conclusion: Serum IL-10 levels may serve as complementary tumor markers and contr...
Medicine Updates
Background: Venous leg ulcers (VLU) consider the commonest type of leg ulceration; venous insuffi... more Background: Venous leg ulcers (VLU) consider the commonest type of leg ulceration; venous insufficiency is the main cause of it. Intra lesion injection of Platelet-rich plasma (PRP) is a potential factor for promoting ulcer healing by the effect of growth factors (GF). The aim was to evaluate the efficacy and safety of (PRP) injection in chronic non-healing (VLU) versus conventional compression therapy. Methods: a randomized prospective comparative study, in 42 patients were managed by dividing into two equal groups; Group (1) managed by PRP injection inside the VLU after preparing the ulcer for healing, and relieving the causes of non-healing ulcer (infection and necrotic tissues or venous insufficiency). Group (2) managed the ulcers by conventional compression therapy. All patients were assisted for 3, 6,9 and 12 months or until wound healing. The injection was performed weekly and assessed granulation tissue healing. Results: The mean percentage of wound closed of PRF injection group was discovered significantly higher than conventional group. With statistically difference in rate of healing ulcer between the PRF group and control group. The mean of reduction in ulcer area in group (1) within 12 months was 1.2 + 0.5, and in group (2) was 3.9 + 1.6. the rate of improvement was 92.7% and 77.1% respectively (P value <0.05). Conclusions: Injection of PRP inside chronic VLU showed better clinical outcomes in comparing with conventional compression therapy alone.
Egyptian Journal of Bronchology, 2015
Context The first step in the diagnostic work up of pleural effusion is the distinction between t... more Context The first step in the diagnostic work up of pleural effusion is the distinction between transudative and exudative pleural effusions (TPEs and EPEs). This discrimination is based on some biochemical tests that are relatively costly and time consuming. Lymphocyte-predominant EPE is the result of many diseases with malignancy, tuberculosis being the most common among them. Aims The aim of this study was to assess the role of pleural fluid viscosity in the differentiation between exudates and transudates and to identify the cause of pleural effusion. Patients and methods The study comprised 10 patients with TPE and 48 patients with EPE: 18 of them had tuberculous (TB) effusion, 25 patients had malignant pleural effusion (MPE) (patients with MPE included 10 with lung cancer and 15 with other known or unknown cancers) and five patients had connective tissue disease (CTD)-associated effusion. Pleural fluid protein, albumin, lactic dehydrogenase, and viscosity were measured in all ...
Suez Canal University Medical Journal
Background: Helicobacter pylori (H. pylori) is one of the most common chronic bacterial infection... more Background: Helicobacter pylori (H. pylori) is one of the most common chronic bacterial infections in humans and has a major etiologic role for several gastroduodenal diseases. Interleukin-6 (IL-6) is a multifunctional cytokine and H. Pylori may stimulate its production. Aim: to assess the serum level of IL-6 in H. pylori infected patients and to study its relation to gastric endoscopy findings. Subjects and Patients and Methods: Eighty-four participants were enrolled; 42 patients with positive H. pylori (patient group "B"), and 42 age and gender-matched subjects with negative H. pylori (control group "A"). Out of 42 patients in the group B, 21 patients were subjected to upper gastrointestinal endoscopy due to persistent gastric upset. Serum concentration of IL-6 was measured in all subjects by ELISA. Results: serum IL-6 was significantly higher in patients' group compared to control group (4.3 ± 0.7 vs 1.5 ± 0.3 pg/ml, p <0.05). The prevalence of IL-6 in infected patient with H. Pylori was 92%, with highest levels in those with gastric ulcer. Conclusion: H. Pylori infection is associated with high serum level of IL-6 especially in those with gastric ulcer.
Afro-Egyptian Journal of Infectious and Endemic Diseases
Background and study aim: Glypican-3 (GPC3) is common kind and new type of the Glypicans group. T... more Background and study aim: Glypican-3 (GPC3) is common kind and new type of the Glypicans group. These groups were connected to the epithelial cell membrane by a glycosyl-phosphatidylinositol bond. These proteins control the signaling action of various growth factors, especially Wnts. This reaction is predicated on the power of glypican to initiate, promote or suppress the reaction of these growth agents with their interactive signaling receptors. It is obviously proven and documented that GPC3 is secreted and released by most malignant liver cells, this glypican is not isolated from healthy hepatocyte, cirrhotic liver cells , or in even in benign liver masses. GPC3 accelerates the development of malignant liver lesions especially HCC by stimulating canonical Wnt impulses. The study aimed to characterize and assess the diagnostic accuracy of serum glypican-3 (GPC3) in early detection of HCC in cirrhotic patients and could be used as good screening marker for HCC in cirrhotic patients instead of AFP. Patients and Methods: We enrolled 60 patients which divided into 2 groups, group1 which included 30 patients diagnosed to have HCC and group 2 which included 30 patients diagnosed to have liver cirrhosis. Results: Our results revealed increased levels of Glypican-3 in hepatoma group and liver cirrhosis group with no significant difference (p=0.3). Conclusion: Serum GPC3 is not an efficient immune-marker for HCC that can be used alone to differentiate HCC from benign hepatic focal lesions, particularly hepatocellular adenoma.
Suez Canal University Medical Journal
Background: Low density lipoprotein receptor (LDLR) has been proposed as a candidate receptor for... more Background: Low density lipoprotein receptor (LDLR) has been proposed as a candidate receptor for hepatitis C virus (HCV). Free beta-lipoproteins in a human serum may regulate the rate of hepatocyte infection by competing with the virus. Therefore, serum HCV levels should be regulated by rise and fall of serum beta-lipoproteins since the infection rate of virions influences HCV replication in hepatocytes and release of virions by hepatocytes. Aim: To evaluate effect of lowdensity lipoprotein levels on the measurement of hepatitis C viral load in chronic hepatitis C patients and estimate the levels of viral load of hepatitis C in relation to variation of the corresponding low-density lipoprotein levels in chronic hepatitis C patients and their relations to each other. Subjects and Methods: 30 HCV hyperlipidemic patients were subjected to clinical evaluation and laboratory investigations included follow up by repeated measurements of LDL level, AST, ALT, and Albumin for patient selection. Results: There was an inverse statistically significant correlation (r =-0.388, p=0.034) between the HCV RNA absolute viral load differences and LDL level absolute differences (the lower the LDL differences, the higher the HCV RNA differences). Also, there was an inverse statistically significant correlation (r=-0.42, p=0.021) between HCV RNA absolute differences and LDL level percent differences (the higher the LDL percent differences, the lower the HCV RNA absolute differences).The predictive variables for HCV RNA viral load level differences were LDL level differences and LDL percent differences whereas AST, ALT, and Albumin differences were not. Conclusion: Low-density lipoprotein levels have an effect on the measurement of hepatitis C viral load in chronic hepatitis C patients. Therefore, this must be taken in consideration when chronic hepatitis C patients have a history of dyslipidemia and perform a HCV RNA viral load by PCR.
Background: The risk for diabetic nephropathy in type 2 diabetes is about 30-40%, and it is consi... more Background: The risk for diabetic nephropathy in type 2 diabetes is about 30-40%, and it is considered the leading cause of end-stage renal disease. Small dense low-density lipoprotein (sdLDL) particles are believed to be atherogenic, and its predominance has been accepted as an emerging cardiovascular risk factor. This study aimed to assess small dense LDL as a potential risk factor and a possible predictor for diabetic nephropathy in type 2 diabetic patients. Patients and Methods: According to microalbuminuria test, 40 diabetic patients were categorized into two groups: Diabetic patients without nephropathy (microalbuminuria negative group) and diabetic patients with nephropathy (microalbuminuria positive group), each group consists of 20 patients and all were non-obese and normotensive. The patients were re-classifi ed into three subgroups depending on the glomerular fi ltration rate (GFR). Results: The mean of small dense LDL level in the microalbuminuria positive group was higher than that in the microalbuminuria negative group, but without statistical signifi cance. It was signifi cantly higher in patients with either mild or moderate decrease in estimated GFR than in patients with normal estimated GFR. There was statistically signifi cant correlation between small dense LDL and albuminuria and signifi cant inverse correlation between small dense LDL and estimated GFR in all patients in the study. Based on microalbuminuria, the sensitivity and specifi city of small dense LDL in the diagnosis of diabetic nephropathy was 40% and 80%, respectively, with cutoff values of small dense LDL >55.14 mg/dl. On the other hand, based on GFR, the sensitivity and specifi city were 88.24% and 73.91% respectively, with cutoff values of small dense LDL >41.89 mg/dl. Conclusion: Small dense LDL is correlated with the incidence and severity of diabetic nephropathy in type 2 diabetic patients. It should be considered as a potential risk factor and as a diagnostic biomarker to be used in conjunction with other biochemical markers for early diagnosis, assessment, and follow-up of diabetic nephropathy.
European Journal of Gastroenterology & Hepatology
Afro-Egyptian Journal of Infectious and Endemic Diseases
Results: The maximum cutoff levels for GP73 was 534.5 ng/L and in comparison the cutoff AFP level... more Results: The maximum cutoff levels for GP73 was 534.5 ng/L and in comparison the cutoff AFP level was 32 ng/mL. For GP73, the sensitivity was 88% and specificity 88% while the sensitivity of AFP was 72% and the specificity of AFP was 80%. The +v predictive value of GP73 and the-ve predictive value of GP73 were 84.6% and 87.5% while the same values of AFP were 75% and 73.1%, respectively. Conclusion: It was found that serum GP73 was up-regulated in HCC and we can use it as a good biomarker for early detection and for screening of malignant liver lesions in cirrhotic patients due chronic HCV infection and it would be more accurate and effective tool than serum AFP in discriminating the malignant liver lesion from the chronic hepatitis.
Afro-Egyptian Journal of Infectious and Endemic Diseases
Results: The maximum cutoff levels for GP73 was 534.5 ng/L and in comparison the cutoff AFP level... more Results: The maximum cutoff levels for GP73 was 534.5 ng/L and in comparison the cutoff AFP level was 32 ng/mL. For GP73, the sensitivity was 88% and specificity 88% while the sensitivity of AFP was 72% and the specificity of AFP was 80%. The +v predictive value of GP73 and the-ve predictive value of GP73 were 84.6% and 87.5% while the same values of AFP were 75% and 73.1%, respectively. Conclusion: It was found that serum GP73 was up-regulated in HCC and we can use it as a good biomarker for early detection and for screening of malignant liver lesions in cirrhotic patients due chronic HCV infection and it would be more accurate and effective tool than serum AFP in discriminating the malignant liver lesion from the chronic hepatitis.
Indian Journal of Endocrinology and Metabolism, 2014
The risk for diabetic nephropathy in type 2 diabetes is about 30-40%, and it is considered the le... more The risk for diabetic nephropathy in type 2 diabetes is about 30-40%, and it is considered the leading cause of end-stage renal disease. Small dense low-density lipoprotein (sdLDL) particles are believed to be atherogenic, and its predominance has been accepted as an emerging cardiovascular risk factor. This study aimed to assess small dense LDL as a potential risk factor and a possible predictor for diabetic nephropathy in type 2 diabetic patients. According to microalbuminuria test, 40 diabetic patients were categorized into two groups: Diabetic patients without nephropathy (microalbuminuria negative group) and diabetic patients with nephropathy (microalbuminuria positive group), each group consists of 20 patients and all were non-obese and normotensive. The patients were re-classified into three sub-groups depending on the glomerular filtration rate (GFR). The mean of small dense LDL level in the microalbuminuria positive group was higher than that in the microalbuminuria negative group, but without statistical significance. It was significantly higher in patients with either mild or moderate decrease in estimated GFR than in patients with normal estimated GFR. There was statistically significant correlation between small dense LDL and albuminuria and significant inverse correlation between small dense LDL and estimated GFR in all patients in the study. Based on microalbuminuria, the sensitivity and specificity of small dense LDL in the diagnosis of diabetic nephropathy was 40% and 80%, respectively, with cutoff values of small dense LDL &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;55.14 mg/dl. On the other hand, based on GFR, the sensitivity and specificity were 88.24% and 73.91% respectively, with cutoff values of small dense LDL &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;41.89 mg/dl. Small dense LDL is correlated with the incidence and severity of diabetic nephropathy in type 2 diabetic patients. It should be considered as a potential risk factor and as a diagnostic biomarker to be used in conjunction with other biochemical markers for early diagnosis, assessment, and follow-up of diabetic nephropathy.
Fertility and sterility, 2008
To evaluate the effects of gonadotropin on angiogenesis by assessing vascular endothelial growth ... more To evaluate the effects of gonadotropin on angiogenesis by assessing vascular endothelial growth factor (VEGF) expression in rat ovaries transplanted after freezing and thawing. In vitro laboratory experiments. Academic research institute. Sixty immature female rats. Frozen-thawed ovaries were autotransplanted into the SC tissue of 60 rats (ages between 21 and 28 days). After transplantation, either pregnant mare&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s serum gonadotropin (PMSG) or saline was administered. The grafted ovaries were collected 2, 7, and 30 days after transplantation for evaluation. Assessment of the morphology and number of follicles, evaluation of apoptosis, and analysis of VEGF expression in the grafted ovaries. Most follicles in the grafts were apoptotic on day 2 but recovered by day 7. The proportion of antral follicles and corpora lutea in the graft correlated with the duration after transplantation. A significant increase in the expression of VEGF188 mRNA was noticed in the grafted ovaries on day 2. Moreover, the mRNA expression in the PMSG group was higher than that in the control group. The increased VEGF protein production in the graft was confirmed by Western blot analysis. In ovariectomized animals, gonadotropin treatment may not provide any added benefits for folliculogenesis and angiogenesis. Nevertheless, a significant increase in the VEGF188 isoform in the gonadotropin-treated group may suggest the positive effect of exogenous gonadotropin therapy in the early stages of angiogenesis.
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Papers by Basma Badreldin