Diabetes mellitus is one of the major lives threatening metabolic disorder all over the world. To... more Diabetes mellitus is one of the major lives threatening metabolic disorder all over the world. To combat this disease, natural small molecules derived from plant plays a key role in diabetes therapy.Cyclin dependent Kinase 5 is a serine-threonine kinase expressed in pancreatic beta cells plays an important role in the pathogenesis of diabetes mellitus .Cdk5 prevents the secretion of insulin by phosphorylating voltage-dependent calcium channels leading tothe decreased concentration of cytosolic Ca2+. The present study involves in silico docking of 2 Tropylpropanaltosylhydrazone a compound isolated from Lannea coromendalica (Houtt.) Merr as inhibitor of Cdk5. While comparing the results from the docking experiments and their interactions with Cdk5, 2 Tropylpropanaltosylhydrazone and Roscovitine the known Cdk5 inhibitor have the binding energy of −9.15 kcal and −6.27 kcal respectively. Moreover the compound satisfies the Lipinski rule and hence it maybe a potential lead compound in the...
Endophytes are microorganisms that reside asymptomatically in the tissues of higher plants and ar... more Endophytes are microorganisms that reside asymptomatically in the tissues of higher plants and are relatively unstudied and a promising source of novel organic natural metabolites exhibiting a variety of biological activities. The intent of this review is to provide insights into the presence of endophytes in nature : their relationship with the host plants, isolation and particularly on the role of endophytes in the production of anticancer, antimicrobial, and antifungal compounds and includes examples that illustrate their potential for human use.
Reverse micelles are discrete nanoscale particles composed of a water core surrounded by surfacta... more Reverse micelles are discrete nanoscale particles composed of a water core surrounded by surfactant. In this current study the the self assembling properties of the dipeptide Boc-Ile-Ile-NHMe in chloroform to form a stable micelle at various temperatures ranging from 200 to 350 Kelvin has been analysed using insilico methods. The computational analysis was carried out using the steepest descent algorithm, a minimization tool used to study the protein energy level in insilco and it was compared with the thermodynamic parameters determined experimentally. Such reverse micelles finds a vast area of application one of which is drug delivery in nanotechnology. The present dipeptide is shown to carry drugs by insilico methods.
Background: Reactive oxygen species (ROS)are produced by host phagocytes and can attack a diverse... more Background: Reactive oxygen species (ROS)are produced by host phagocytes and can attack a diverse range of targets to exert antimicrobial activity, against a broad range of pathogens. Four major ROS are recognized comprising superoxide, hydrogen peroxide, hydroxyl radical (• OH), and singlet oxygen. Antioxidant enzymes such as glutathione peroxidase (GPX), superoxide dismutase (SOD) and glutathione reductase (GSH) plays a crucial role by protecting the organism from endogenous ROS. This work is designed to find out the dominant secondary metabolites derived from the fungus Aspergillus sp. isolated from Lannea coramendalica and to elucidate antimicrobial activity through interaction with specifically intracellular targets. Materials and Methods: Aspergillus sp. was isolated and inoculated in a production media to extract secondary metabolite by using ethyl alcohol as solvent. The filtrate was analysed for secondary metabolites by using gas chromatography/mass spectrometry (GC-MS) ana...
In recent years endophytic fungi from plants have gained tremendous importance with emerging evid... more In recent years endophytic fungi from plants have gained tremendous importance with emerging evidence of their high biotechnological potential for their valuable source of useful metabolites. In the present study an attempt has been made to isolate endophytes associated with leaves and bark of Lannea coromandelica(Houtt.) Merr (Anacardiaceae).Five hundred bark and leaf segments were analysed and a total of 586 isolates representing 20 taxa, with a colonization rate of 59.6% were isolated. A total of 586 isolates belonging to 20 taxa comprising 5.70% Ascomycetes, 5.20% Zygomycetes, 18.45% Coelomycetes, 67.78% Hypomycetes, 2.85% sterile mycelia were obtained. Species of, Aspergillus, Alternaria,and Colletotrichum were isolated as dominant endophytes. Colonization frequency of Aspergillus flavus(21.64 %) was highest which was isolated from all the tissues.
Diabetes mellitus is one of the major lives threatening metabolic disorder all over the world. To... more Diabetes mellitus is one of the major lives threatening metabolic disorder all over the world. To combat this disease, natural small molecules derived from plant plays a key role in diabetes therapy.Cyclin dependent Kinase 5 is a serine-threonine kinase expressed in pancreatic beta cells plays an important role in the pathogenesis of diabetes mellitus .Cdk5 prevents the secretion of insulin by phosphorylating voltage-dependent calcium channels leading tothe decreased concentration of cytosolic Ca2+. The present study involves in silico docking of 2 Tropylpropanaltosylhydrazone a compound isolated from Lannea coromendalica (Houtt.) Merr as inhibitor of Cdk5. While comparing the results from the docking experiments and their interactions with Cdk5, 2 Tropylpropanaltosylhydrazone and Roscovitine the known Cdk5 inhibitor have the binding energy of −9.15 kcal and −6.27 kcal respectively. Moreover the compound satisfies the Lipinski rule and hence it maybe a potential lead compound in the...
Endophytes are microorganisms that reside asymptomatically in the tissues of higher plants and ar... more Endophytes are microorganisms that reside asymptomatically in the tissues of higher plants and are relatively unstudied and a promising source of novel organic natural metabolites exhibiting a variety of biological activities. The intent of this review is to provide insights into the presence of endophytes in nature : their relationship with the host plants, isolation and particularly on the role of endophytes in the production of anticancer, antimicrobial, and antifungal compounds and includes examples that illustrate their potential for human use.
Reverse micelles are discrete nanoscale particles composed of a water core surrounded by surfacta... more Reverse micelles are discrete nanoscale particles composed of a water core surrounded by surfactant. In this current study the the self assembling properties of the dipeptide Boc-Ile-Ile-NHMe in chloroform to form a stable micelle at various temperatures ranging from 200 to 350 Kelvin has been analysed using insilico methods. The computational analysis was carried out using the steepest descent algorithm, a minimization tool used to study the protein energy level in insilco and it was compared with the thermodynamic parameters determined experimentally. Such reverse micelles finds a vast area of application one of which is drug delivery in nanotechnology. The present dipeptide is shown to carry drugs by insilico methods.
Background: Reactive oxygen species (ROS)are produced by host phagocytes and can attack a diverse... more Background: Reactive oxygen species (ROS)are produced by host phagocytes and can attack a diverse range of targets to exert antimicrobial activity, against a broad range of pathogens. Four major ROS are recognized comprising superoxide, hydrogen peroxide, hydroxyl radical (• OH), and singlet oxygen. Antioxidant enzymes such as glutathione peroxidase (GPX), superoxide dismutase (SOD) and glutathione reductase (GSH) plays a crucial role by protecting the organism from endogenous ROS. This work is designed to find out the dominant secondary metabolites derived from the fungus Aspergillus sp. isolated from Lannea coramendalica and to elucidate antimicrobial activity through interaction with specifically intracellular targets. Materials and Methods: Aspergillus sp. was isolated and inoculated in a production media to extract secondary metabolite by using ethyl alcohol as solvent. The filtrate was analysed for secondary metabolites by using gas chromatography/mass spectrometry (GC-MS) ana...
In recent years endophytic fungi from plants have gained tremendous importance with emerging evid... more In recent years endophytic fungi from plants have gained tremendous importance with emerging evidence of their high biotechnological potential for their valuable source of useful metabolites. In the present study an attempt has been made to isolate endophytes associated with leaves and bark of Lannea coromandelica(Houtt.) Merr (Anacardiaceae).Five hundred bark and leaf segments were analysed and a total of 586 isolates representing 20 taxa, with a colonization rate of 59.6% were isolated. A total of 586 isolates belonging to 20 taxa comprising 5.70% Ascomycetes, 5.20% Zygomycetes, 18.45% Coelomycetes, 67.78% Hypomycetes, 2.85% sterile mycelia were obtained. Species of, Aspergillus, Alternaria,and Colletotrichum were isolated as dominant endophytes. Colonization frequency of Aspergillus flavus(21.64 %) was highest which was isolated from all the tissues.
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