Papers by YUKITAKA ISHIMOTO
The Proceedings of Conference of Tohoku Branch
The Proceedings of Conference of Tohoku Branch
The Proceedings of Conference of Tohoku Branch
The Proceedings of Conference of Tohoku Branch
The Proceedings of Conference of Tohoku Branch
The Proceedings of Conference of Tohoku Branch
The Proceedings of Conference of Tohoku Branch
The Proceedings of Conference of Tohoku Branch
The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME
The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME
SummaryAt the level of organ formation, tissue morphogenesis drives developmental processes in an... more SummaryAt the level of organ formation, tissue morphogenesis drives developmental processes in animals, often involving the rearrangement of two-dimensional (2D) structures into more complex three-dimensional (3D) tissues. These processes can be directed by growth factor signaling pathways. However, little is known about how such morphological changes affect the spatiotemporal distribution of growth factor signaling. Here, using the Drosophila pupal wing, we address how Decapentaplegic (Dpp) / Bone Morphogenetic Protein (BMP) signaling and 3D wing morphogenesis are coupled. Dpp, expressed in the longitudinal veins (LVs) of the pupal wing, initially diffuses laterally during the inflation stage to regulate cell proliferation. Dpp localization is then refined to the LVs within each epithelial plane, but with active interplanar signaling for vein patterning, as the two epithelia appose. Our data further suggest that the 3D architecture of the wing epithelia directs the spatial distribu...
The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME
The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME
Proceedings of the National Academy of Sciences
At the level of organ formation, tissue morphogenesis drives developmental processes in animals, ... more At the level of organ formation, tissue morphogenesis drives developmental processes in animals, often involving the rearrangement of two-dimensional (2D) structures into more complex three-dimensional (3D) tissues. These processes can be directed by growth factor signaling pathways. However, little is known about how such morphological changes affect the spatiotemporal distribution of growth factor signaling. Here, using the Drosophila pupal wing, we address how decapentaplegic (Dpp)/bone morphogenetic protein (BMP) signaling and 3D wing morphogenesis are coordinated. Dpp, expressed in the longitudinal veins (LVs) of the pupal wing, initially diffuses laterally within both dorsal and ventral wing epithelia during the inflation stage to regulate cell proliferation. Dpp localization is then refined to the LVs within each epithelial plane, but with active interplanar signaling for vein patterning/differentiation, as the two epithelia appose. Our data further suggest that the 3D archit...
Biophysical Journal
For isolated single cells on a substrate, the intracellular stiffness, which is often measured as... more For isolated single cells on a substrate, the intracellular stiffness, which is often measured as the Young's modulus, E, by atomic force microscopy (AFM), depends on the substrate rigidity. However, little is known about how the E of cells is influenced by the surrounding cells in a cell population system in which cells physically and tightly contact adjacent cells. In this study, we investigated the spatial heterogeneities of E in a jammed epithelial monolayer in which cell migration was highly inhibited, allowing us to precisely measure the spatial distribution of E in large-scale regions by AFM. The AFM measurements showed that E can be characterized using two spatial correlation lengths: the shorter correlation length, l S , is within the single cell size, whereas the longer correlation length, l L , is longer than the distance between adjacent cells and corresponds to the intercellular correlation of E. We found that l L decreased significantly when the actin filaments were disrupted or calcium ions were chelated using chemical treatments, and the decreased l L recovered to the value in the control condition after the treatments were washed out. Moreover, we found that l L decreased significantly when E-cadherin was knocked down. These results indicate that the observed long-range correlation of E is not fixed within the jammed state but inherently arises from the formation of a large-scale actin filament structure via E-cadherin-dependent cell-cell junctions.
The Proceedings of Conference of Tohoku Branch
The Proceedings of Conference of Tohoku Branch
The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME
Seibutsu Butsuri
Bubbly vertex dynamics: 曲率をもつ細胞形状を含む上皮組織 のための幾何学的動力学モデル Bubbly vertex dynamics: a dynamical and g... more Bubbly vertex dynamics: 曲率をもつ細胞形状を含む上皮組織 のための幾何学的動力学モデル Bubbly vertex dynamics: a dynamical and geometrical model for epithelial tissues with curved cell shapes Yukitaka Ishimoto, Yoshihiro Morishita (RIKEN CDB
Journal of theoretical biology, Jan 24, 2017
The wings in different insect species are morphologically distinct with regards to their size, ou... more The wings in different insect species are morphologically distinct with regards to their size, outer contour (margin) shape, venation, and pigmentation. The basis of the diversity of wing margin shapes remains unknown, despite the fact that gene networks governing the Drosophila wing development have been well characterised. Among the different types of wing margin shapes, smoothly curved contour is the most frequently found and implies the existence of a highly organised, multicellular mechanical structure. Here, we developed a mechanical model for diversified insect wing margin shapes, in which non-uniform bending stiffness of the wing margin is considered. We showed that a variety of spatial distribution of the bending stiffness could reproduce diverse wing margin shapes. Moreover, the inference of the distribution of the bending stiffness from experimental images indicates a common spatial profile among insects tested. We further studied the effect of the intrinsic tension of th...
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Papers by YUKITAKA ISHIMOTO