Papers by Karan Pachchigar
![Research paper thumbnail of In Vitro Regeneration of Pigeonpea [Cajanus cajan (L.) Millsp.] Genotype GT 101 using Cotyledonary Node](https://a.academia-assets.com/images/blank-paper.jpg)
Indian Journal of Science and Technology, 2016
Objectives: A protocol was developed for plant regeneration using cotyledonary node of high-yield... more Objectives: A protocol was developed for plant regeneration using cotyledonary node of high-yielding indigenous pigeonpea [Cajanus cajan (L.) Millsp.] genotype GT 101. Methods: Induction of multiple shoots directly was completed from cotyledonary node as an explants on Murashige and Skoog’s (MS) medium supplemented with various concentrations and combination of 6-benzylaminopurine (BAP), kinetin and α-naphthalene acetic acid (NAA). Elongation of multiplied shoots was performed on MS medium supplemented with different combination of BAP and GA3. These well elongated plantlets were further transferred on MS medium supplemented with various concentrations of indole-3-butyric acid (IBA) for root induction. Regenerated plants were transferred to cocopeat:soil:vermiculite (2:1:2) for acclimation. Findings: The frequency of multiplication and number of multiple shoots from cotyledonary node explant were influenced on various types and concentrations of cytokinin. For multiple shoots induction, 3.0 mg/L BAP with 0.5 mg/L NAA was superior as compared to other combinations. The elongation of multiplied shoots was carried out on MS medium supplemented with 0.5 mg/L BAP and 0.5 mg/L GA3. The developed shoots were advanced to rooting on the medium supplemented with 0.5 mg/L IBA. They were subsequently grown in pots with 80% survival rate and these plants produced viable seeds. Improvement: The protocol for the production of in vitro multiple shoots with high frequency and their successive conversion to whole plants agreements potential for use in the improvement of protocol for development of transgenic in pigeonpea.
RAPD assessment on genetic variation of total 58 accessions comprising 15 parental genotypes and ... more RAPD assessment on genetic variation of total 58 accessions comprising 15 parental genotypes and their 43 F 1 hybrids of grain Amaranthus were analyzed. Among the 40 tested primers, four primers viz. OPA-06, OPA-10, OPA-14 and OPA-15 were able to discriminate all the genotypes sufficiently. In RAPD analysis, at the intraspecific level, the percentages of RAPD polymorphism were found to be 71.A. tricolor)] and hybrids showed varied degree of polymorphism ranging from 42.86 to 71.43 per cent. The population matrix showed that allele at SKGPA-144 (Amaranthus tricolor) and F 1 hybrids have maximum genetic distance of 0.742 to 0.962 in all populations while population IC-1733 (Amaranthus edulis) has minimum genetic distance among all the germplasm. The lowest and the maximum genetic distance of IC-1733(Amaranthus edulis) was 0.663 and 0.720 respectively to all populations. It further matches the pattern of allelic frequency distribution where SKGPA-144(Amaranthus tricolor) and F 1 have r...
AFRICAN JOURNAL OF BIOTECHNOLOGY
The reliability of the quantification of genetic diversity using only one type of marker has been... more The reliability of the quantification of genetic diversity using only one type of marker has been questioned as compared to the combined use of different markers. To compare the efficiency of the use of single versus multiple markers, the genetic diversity was quantified among 12 diverse pigeonpea germplasm comprised of eight wild and four cultivated using both random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers, and how well these two types of markers discriminated the diverse pigeonpea germplasm was evaluated. The pigeonpea germplasm including eight wild species and four cultivated varieties was subjected to 40 RAPD and 40 microsatellite primers. The level of polymorphism as revealed by RAPD primers produced a total of 517 DNA fragments and all were found to be polymorphic that is, 100% and in SSR analysis 101 fragments were produced that too showed 100% polymorphism. The high similarity index value revealed by RAPD was 0.931 between GT-100 and ICPL-87...
Biotechnology & genetic engineering reviews, 2014
Long-term research on RNA interference led to an unfathomed understanding of the mechanism of siR... more Long-term research on RNA interference led to an unfathomed understanding of the mechanism of siRNA-mediated silencing and finally siRNA has emerged as a powerful therapeutic tool. With siRNAs virtually every gene in the human genome contributing to a disease becomes amenable to regulation, thus opening unprecedented opportunities for drug discovery. siRNA has a well-established role as a tool for in vitro target screening and validation, besides these recent progresses of siRNA delivery in vivo, this has raised more expectations for siRNA-based drugs as the up-and-coming 'magic bullet'. Although a plethora of articles have been published with siRNA, the fundamentals of siRNA-mediated gene silencing and transforming the functional genomics to novel therapeutics are reviewed in this article with consideration to present hurdles as a new generation challenge.
AFRICAN JOURNAL OF BIOTECHNOLOGY, 2011
Trianthema decandra L. root and root callus extracts of different solvents viz., petroleum ether,... more Trianthema decandra L. root and root callus extracts of different solvents viz., petroleum ether, chloroform, ethyl acetate and ethanol were tested against both Gram positive and Gram negative bacteria and also against Fusarium spp. Root callus extract of chloroform and ethanol showed significant activity against Bacillus subtilis, B. cereus. Staphylococcus aureus, Staph. epidermis and also against the other spp. of Gram negative bacteria viz., Pseudomonas aeruginosa, Klebseilla pneumonia, Alcaligens faecalis, Proteus vulgaris Enterobacter aerogenes, Salmonella typhi, Salmonella tyhimurium, Salmonella paratyphi A and Salmonella enterica subsp. enterica with a MIC of 3.12 to 12.50 µg/ml when compared to root extract of chloroform, ethyl acetate and ethanol of Trianthema decandra. Root callus extract of chloroform, ethyl acetate and ethanol showed activity against Fusarium verticilliodes, F. anthophilum, F. oxysporum and F. proliferatum with a lowest MIC of 3.12 µg/ml when compared to root extract of Trianthema decandra. The result shows that antibacterial and antifungal activity was more in root callus extract than root extract.
Journal of Experimental Biology and Agricultural Sciences, 2017
Stable performance of grain amaranths genotypes at specific environment is critical for obtaining... more Stable performance of grain amaranths genotypes at specific environment is critical for obtaining high and consistent yield. This study was conducted to assess grain yield stability of thirteen genotypes in varied environments in three successive growing seasons and effects of genotype (G) and genotype×environment (GE) interaction were illustrate graphically based on "what-won-where" to advocate genotypes for a specific environment, using GGE biplot. Result of study revealed that GGE biplot was effective to recognize stable and highest yield (1648.92 kg/ha) genotype as G5 followed by G6 (1457.24 kg/ha) and G3 (1268.62 kg/ha) genotypes while the genotype G7 had the lowest grain yield and was the least stable across varying environments. This study could be aid tool for endorsement of amaranths genotypes for specific environment, taking into elucidation the peculiarities of genotypes and growing stipulation.
Stable performance of grain amaranths genotypes at specific environment is critical for obtaining... more Stable performance of grain amaranths genotypes at specific environment is critical for obtaining high and consistent yield. This study was conducted to assess grain yield stability of thirteen genotypes in varied environments in three successive growing seasons and effects of genotype (G) and genotype×environment (GE) interaction were illustrate graphically based on " what-won-where " to advocate genotypes for a specific environment, using GGE biplot. Result of study revealed that GGE biplot was effective to recognize stable and highest yield (1648.92 kg/ha) genotype as G5 followed by G6 (1457.24 kg/ha) and G3 (1268.62 kg/ha) genotypes while the genotype G7 had the lowest grain yield and was the least stable across varying environments. This study could be aid tool for endorsement of amaranths genotypes for specific environment, taking into elucidation the peculiarities of genotypes and growing stipulation.
Objective: The present investigation was performed to evaluate the antimicrobial potential of Tri... more Objective: The present investigation was performed to evaluate the antimicrobial potential of Trigonella foenum-graecum leaves extracts and to elucidate the presence of phytochemicals responsible for its biological activity. Methods: The plant extracts were prepared by sequential cold maceration method by using four solvents viz., hexane, ethyl acetate, methanol and distilled water. Antimicrobial activity of extracts was carried out by agar well diffusion method against four bacteria and five fungi. Minimum inhibitory concentration (MIC) of different extracts was determined using the broth dilution method. Thin layer chromatography (TLC), TLC bioautography and phytochemicals analysis were also performed. Results: The antibacterial activity of T. foenum-graecum leaves extracts was found maximum on Serratia marcescens with a zone of inhibition (ZOI) of 12.33±0.57 mm by aqueous extract followed by inhibition of Bacillus cereus (ZOI = 11.50±0.50 mm) by the methanol extract. The range of MIC of different extracts recorded was 6.25 to 25 mg/ml. Results of the antifungal activity showed that methanol extract showed a maximum zone of inhibition against Trichoderma viridae (ZOI = 14.5±0.5 mm) followed by ethyl acetate extract (ZOI = 12.0±1.0 mm). The MIC value for methanol extract against T. viridae was 6.25 mg/ml. The result of TLC bioautography revealed that compounds eluted at Rf 0.58 and Rf 0.67 exhibited strong while compounds eluted at Rf 0.31 and Rf 0.37 showed moderate antibacterial activity against S. marcescens. Phytochemicals analysis indicated that methanol and aqueous extracts contain more phytochemicals as compared to hexane and ethyl acetate extracts. Conclusion: The results obtained in this study clearly indicate that T. foenum-graecum leaves extracts to have a significant potential to be used as an antimicrobial agent due to the presence of different phytochemicals.
Long-term research on RNA interference led to an unfathomed understanding of the mechanism of siR... more Long-term research on RNA interference led to an unfathomed understanding of the mechanism of siRNA-mediated silencing and finally siRNA has emerged as a powerful therapeutic tool. With siRNAs virtually every gene in the human genome contributing to a disease becomes amenable to regulation, thus opening unprecedented opportunities for drug discovery. siRNA has a well-established role as a tool for in vitro target screening and validation, besides these recent progresses of siRNA delivery in vivo, this has raised more expectations for siRNA-based drugs as the up-and-coming 'magic bullet'. Although a plethora of articles have been published with siRNA, the fundamentals of siRNA-mediated gene silencing and transforming the functional genomics to novel therapeutics are reviewed in this article with consideration to present hurdles as a new generation challenge.
RAPD assessment on genetic variation of total 58 accessions comprising 15 parental genotypes and ... more RAPD assessment on genetic variation of total 58 accessions comprising 15 parental genotypes and their 43 F 1 hybrids of grain Amaranthus were analyzed. Among the 40 tested primers, four primers viz. OPA-06, OPA-10, OPA-14 and OPA-15 were able to discriminate all the genotypes sufficiently. In RAPD analysis, at the intraspecific level, the percentages of RAPD polymorphism were found to be 71.43 (A. hypochondriacus), 100 (A. cruentus), 100 (A. caudatus), 75.76 [IC-1733 (A. edulis)] and 84.85 [SKGPA-144 (A. tricolor)] and hybrids showed varied degree of polymorphism ranging from 42.86 to 71.43 per cent. The population matrix showed that allele at SKGPA-144 (Amaranthus tricolor) and F 1 hybrids have maximum genetic distance of 0.742 to 0.962 in all populations while population IC-1733 (Amaranthus edulis) has minimum genetic distance among all the germplasm. The lowest and the maximum genetic distance of IC-1733(Amaranthus edulis) was 0.663 and 0.720 respectively to all populations. It further matches the pattern of allelic frequency distribution where SKGPA-144(Amaranthus tricolor) and F 1 have remarkable high polymorphism.
The reliability of the quantification of genetic diversity using only one type of marker has been... more The reliability of the quantification of genetic diversity using only one type of marker has been questioned as compared to the combined use of different markers. To compare the efficiency of the use of single versus multiple markers, the genetic diversity was quantified among 12 diverse pigeonpea germplasm comprised of eight wild and four cultivated using both random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers, and how well these two types of markers discriminated the diverse pigeonpea germplasm was evaluated. The pigeonpea germplasm including eight wild species and four cultivated varieties was subjected to 40 RAPD and 40 microsatellite primers. The level of polymorphism as revealed by RAPD primers produced a total of 517 DNA fragments and all were found to be polymorphic that is, 100% and in SSR analysis 101 fragments were produced that too showed 100% polymorphism. The high similarity index value revealed by RAPD was 0.931 between GT-100 and ICPL-87 whereas through SSR, it was 1.00 between GTH-1 and GT-100 as well as Rhyncosia rothi and Rhyncosia minima. The least similarity index value revealed by RAPD (R. rothi and GTH-1) and SSR (Rhyncosia bracteata and ICPL-87) were 0.07 and 0.133, respectively. Using RAPD marker, the calculated arithmetic mean heterozygosity and the marker index were 0.90 and 22.47, respectively. The R. bracteata and ICPL-87 were found distinct from rest of other cultivars by showing only 13% similarity. Average PIC value shown by RAPD and SSR primers were found to be 0.90 and 0.18, respectively.
Content Abstract Preamble Objective Methodology Result Discussion conclusion Abstract As... more Content Abstract Preamble Objective Methodology Result Discussion conclusion Abstract As natural antioxidants are better cure for inflammation, in the present study we have explored the antioxidant potential of four different herbal extracts from local Maharashtra region. Study done for NO scavenging of extract A showed 84.33 % while for anti lipid peroxidation potential showed 509 % reduction for extract D in dose dependent manner. Cytotoxicity analysis of extract A was done in MG 63 cell line and showed 100% viability suggesting it safer to cells. Analysis of NO release after dosing with extract A on cells done for different concentration showed 3.83 fold ( 73.88 %) decrease in NO release at 80 µg/ml. Molecular analysis to check expression of NO was carried out on control (untreated) cells.
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Papers by Karan Pachchigar