Papers by Michelle Van Noy
Toxoplasma gondii infection causes severe and lethal disease on fetus, AIDS patients and recipien... more Toxoplasma gondii infection causes severe and lethal disease on fetus, AIDS patients and recipients of organ transplants. There are few reports of the use of ionizing radiation to attenuate0 or abolish parasite growth, without any detailed study on induced alterations or effective doses. We reported that the lower dose of 60 Co radiation that abolishes parasite growth was 200 Gy, and in this report, we described the viability, cell invasion, metabolism and immunogenicity of this parasite after irradiation. We analyze the viability (vital staining), metabolism, by MTT oxidative conversion, protein metabolism, by 3 H-proline and nucleic acid synthesis, by 3 H-hypoxanthine incorporation in short terms cultures. Parasite invasion was tested in LLC-MK2 cell in culture. The irradiated parasites show the same viability and invasiveness of viable parasites, without any interference of the radiation in the oxidative, proteic or nucleic acid metabolism. When mice injected with 10 7 irradiated parasites by intraperitoneal route, were challenged 6 weeks later with viable parasites, they showed partial resistance to infection, with higher survival time. Antibody specificity was tested in sera from those animals, before challenging, and presented the same epitopic specificity of infected and treated animals, but diverse from mice injected with formaldehyde killed parasite, by Western-blot analysis against SDS-PAGE isolated T.gondii antigens. Those data suggests that irradiated parasites maintain its invasiveness, inducing a partial immunity and similar humoral immunogenicity than viable parasites, but without any evidence of reproductive capacity.
Community College Research Center Columbia University, Oct 1, 2012
Using data obtained from interviews and program websites at Washington community and technical co... more Using data obtained from interviews and program websites at Washington community and technical colleges, the authors of this study examine the structure of community college career-technical programs in allied health, business and marketing, computer and information studies, and mechanics and repair. A framework for structure with four dimensions-program alignment, program prescription, information quality, and active program advising and support-is used to evaluate the practices of relatively high-and low-performing colleges within each field of study. The authors reviewed the websites of all programs at high-and low-performing colleges in each of these fields of study and conducted case studies on individual programs from these fields, interviewing faculty, administrators, and counselors to learn more about the dimensions of structure in the programs. The allied health, computer and information science, and mechanics and repair programs were all found to be highly structured; the business and marketing programs were found to have a moderate level of structure. Overall, given that all of the programs were at least moderately structured, there was limited evidence of a connection between program structure and program performance.
Community College Research Center Columbia University, Sep 1, 2010
of MPR Associates for helpful comments and suggestions on earlier drafts. We thank Amy Mazzariell... more of MPR Associates for helpful comments and suggestions on earlier drafts. We thank Amy Mazzariello of CCRC for her excellent work in editing the document. All errors are our own.
Uploads
Papers by Michelle Van Noy